ViewGene
Locus Name:
GeneID:
Location:

GeneID:4978
TrivialName: PA14_10970
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_10970
PAO1 Ortholog Locus:
Sequence Length:966
Protein Length:321
Start: 949493
Stop: 948528
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAA26020.1| (M22077) ferredoxin [Pseudomonas sp.]
Identities = 144/321 (44%), Positives = 180/321 (56%), Gaps = 16/321 (4%)
Sequence:
ATGAGCGCATCGCCTTCCTCCCGCCTGCACCTGCAGGTCACCGATTTCCGCGCTGTTGCGCGCGACGTGGTG
CTGGTCGAGCTGGCCGCCGTGGACGACACCGAACTGCCGGCCTTCACCCCCGGCGCGCATCTGGAGCTTCAC
CTGGGCAACGGCCTGATCCGCCACTACTCCCTGCTCAACGACTGCCGCGAACGCCAGCGCTACCAACTGGCG
ATAGGCCTTGGCGACGACAGCCGTGGCGGCGCCCGCTTCATCCACCGCCAACTGCGCACGGGCGACCGCCTG
CACGCGGCGGCACCGCGCAACCACTTCATGCTGCGCGAAGACGCCGCGCGCTACTGTTTCGTCGCTGGCGGC
ATCGGCATCACCCCGATTCTCTCGATGGTTCGCTGGTGCCAGGCGCAGGGCAAGGACTGGCGCCTACTGTAT
GCGGTCCGCGAACGCCAGCGCGCAGCCTTCCTCGCCGAACTGATGACGCTCGGTGGCGAACGGACGCGGCTG
CATTGCAGCGACGAAGGCGACCCACTCGACAGTACCGCGCTGATCGCCGGCCTAGGTGCCGACGAAGAACTC
TATTGCTGCGGACCCGACGGGTTGATGCAGGCCATCCGCGACGCCGGGAGCGGCTGCGCGGAACGCCTGCAC
TTCGAACGCTTCGACGCTCCAGTAGCGCCGGCGTCCTCGTCCGGCGGCACCGACGCCTTCCGCCTGGAGTTG
CGCCGCAGCGCCCTGAGCCTGCGCGTCGAAAGCCACCAGAGCATCCTCGAAGTCCTCGAGGAACAGGGCCTG
GAGCCTCCCTACGCCTGCCGCGCCGGGATATGCAGGACCTGCGAGACACGCGTCTGCGCCGGCGAGCCGGAG
CACTTCGACCATGTGCTTTCCGACACCGAGCGGGCCAGCGGGGAAACCTTGCTGATCTGCGTATCACGCTGC
CGCGGCAACTACCTGGAACTCGATCTCTGA
Translation:
MSASPSSRLHLQVTDFRAVARDVVLVELAAVDDTELPAFTPGAHLELHLGNGLIRHYSLLNDCRERQRYQLA
IGLGDDSRGGARFIHRQLRTGDRLHAAAPRNHFMLREDAARYCFVAGGIGITPILSMVRWCQAQGKDWRLLY
AVRERQRAAFLAELMTLGGERTRLHCSDEGDPLDSTALIAGLGADEELYCCGPDGLMQAIRDAGSGCAERLH
FERFDAPVAPASSSGGTDAFRLELRRSALSLRVESHQSILEVLEEQGLEPPYACRAGICRTCETRVCAGEPE
HFDHVLSDTERASGETLLICVSRCRGNYLELDL*
AnnotationID:4983GeneID:4978
AnnotatorUID: danlee
Modification Date/Time: 2005-10-19 15:03:17
Gene Name:
Confidence Code:3
GeneProduct:putative flavodoxin reductase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1018, cd00207
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
gi|67909931|ref|ZP_00508325.1|  Ferredoxin:Oxidoreductase FAD/NAD(P)-binding:Oxidoreductase FAD-binding 
region [Polaromonas sp. JS666]
     Length=319
     Score =  247 bits (631),  Expect = 3e-64
     Identities = 142/320 (44%), Positives = 191/320 (59%), Gaps = 6/320 (1%)

gi|73537414|ref|YP_297781.1|  Ferredoxin:Oxidoreductase FAD/NAD(P)-binding:Oxidoreductase FAD-binding 
region [Ralstonia eutropha JMP134]
gi|53761861|ref|ZP_00167575.2|  COG1018: Flavodoxin reductases (ferredoxin-NADPH reductases) 
family 1 [Ralstonia eutropha JMP134]
     Length=318
     Score =  245 bits (626),  Expect = 1e-63
     Identities = 137/320 (42%), Positives = 190/320 (59%), Gaps = 5/320 (1%)

gi|137443|sp|P12580|VANB_PSES9  Vanillate O-demethylase oxidoreductase (Vanillate degradation 
ferredoxin-like protein)
gi|279988|pir||B43652  ferredoxin [2Fe-2S] homolog vanB - Pseudomonas sp
gi|151637|gb|AAA26020.1|  ferredoxin
     Length=314
     Score =  234 bits (598),  Expect = 2e-60
     Identities = 144/321 (44%), Positives = 180/321 (56%), Gaps = 16/321 (4%)







 
Structural Features:
COG1018, Hmp, Flavodoxin reductases (ferredoxin-NADPH reductases) family 1 [Energy production 
and conversion].
             CD-Length = 266 residues,  88.3% aligned
             Score =  130 bits (328), Expect = 3e-31

COG0543, UbiB, 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases [Coenzyme 
metabolism / Energy production and conversion].
             CD-Length = 252 residues,  71.4% aligned
             Score = 67.4 bits (164), Expect = 2e-12

C-Terminus:
cd00207, fer2, 2Fe-2S iron-sulfur cluster binding domain. 
             CD-Length = 84 residues,  76.2% aligned
             Score = 61.2 bits (148), Expect = 1e-10






   
Genomic Context:






   
Comment:
Identities = 144/321 (44%), Positives = 180/321 (56%), Gaps = 16/321 (4%)
when compared to PA3498 (probable oxidoreductase) of PAO1.
No better match to PA3498 found in PA14 genome.



















    ReferenceID:9956




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17070




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4978_CDD.html


    		




 
URL:

















    ReferenceID:27097




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:4978
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
191261
                           gb|AAA26020.1|
                           (M22077) ferredoxin [Pseudomonas sp.]
                           330
                           43.93
                           54.54
                           25
                           615.0
     

     
     
7384
                           PA3498_tr
                           translation of PA3498
                           328
                           42.07
                           56.40
                           17
                           600.0
     

     
     
191262
                           gb|AAG06886.1|AE004770_11
                           (AE004770) probable oxidoreductase [Pseudomonas aeruginosa PAO1]
                           328
                           42.07
                           56.40
                           17
                           600.0
     

     
     
191266
                           emb|CAA19957.1|
                           (AL939128) iron-sulfur oxidoreductase beta subunit [Streptomyces coelicolor A3(2)]
                           326
                           41.71
                           55.82
                           18
                           583.0
     

     
     
191263
                           gb|AAK37997.1|
                           (AF305549) reductase TsaB2 [Comamonas testosteroni]
                           330
                           40.90
                           57.27
                           22
                           595.0
     

     






      

          
GC ORFID: 47980How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3498Glimmer Score: 

          
Start: 4004444Stop: 4005409

          
Length: 966


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3498 translation of PA3498Homolog Bit Score 229.0

          
Other Homologs:  t_PA4905 (ORF: BLASTX 211.0), t_PA3498 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCGCATCGCCTTCCTCCCGCCTGCACCTGCAGGTCACCGATTTCCGCGCTGTTGCGCGCGACGTGGTG 
CTGGTCGAGCTGGCCGCCGTGGACGACACCGAACTGCCGGCCTTCACCCCCGGCGCGCATCTGGAGCTTCAC 
CTGGGCAACGGCCTGATCCGCCACTACTCCCTGCTCAACGACTGCCGCGAACGCCAGCGCTACCAACTGGCG 
ATAGGCCTTGGCGACGACAGCCGTGGCGGCGCCCGCTTCATCCACCGCCAACTGCGCACGGGCGACCGCCTG 
CACGCGGCGGCACCGCGCAACCACTTCATGCTGCGCGAAGACGCCGCGCGCTACTGTTTCGTCGCTGGCGGC 
ATCGGCATCACCCCGATTCTCTCGATGGTTCGCTGGTGCCAGGCGCAGGGCAAGGACTGGCGCCTACTGTAT 
GCGGTCCGCGAACGCCAGCGCGCAGCCTTCCTCGCCGAACTGATGACGCTCGGTGGCGAACGGACGCGGCTG 
CATTGCAGCGACGAAGGCGACCCACTCGACAGTACCGCGCTGATCGCCGGCCTAGGTGCCGACGAAGAACTC 
TATTGCTGCGGACCCGACGGGTTGATGCAGGCCATCCGCGACGCCGGGAGCGGCTGCGCGGAACGCCTGCAC 
TTCGAACGCTTCGACGCTCCAGTAGCGCCGGCGTCCTCGTCCGGCGGCACCGACGCCTTCCGCCTGGAGTTG 
CGCCGCAGCGCCCTGAGCCTGCGCGTCGAAAGCCACCAGAGCATCCTCGAAGTCCTCGAGGAACAGGGCCTG 
GAGCCTCCCTACGCCTGCCGCGCCGGGATATGCAGGACCTGCGAGACACGCGTCTGCGCCGGCGAGCCGGAG 
CACTTCGACCATGTGCTTTCCGACACCGAGCGGGCCAGCGGGGAAACCTTGCTGATCTGCGTATCACGCTGC 
CGCGGCAACTACCTGGAACTCGATCTCTGA