ViewGene
Locus Name:
GeneID:
Location:

GeneID:496
TrivialName: PA14_15030
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_15030
PAO1 Ortholog Locus:PA3792
Sequence Length:1779
Protein Length:592
Start: 1275652
Stop: 1273874
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07179.1|AE004798_2 (AE004798) 2-isopropylmalate synthase [Pseudomonas aeruginosa PAO1]
Identities = 590/592 (99%), Positives = 591/592 (99%)
Sequence:
GTGCCTGGTCAGCCCGCGACTGCGTGGCTGAGCGACGCTTCTCTTCATCTCGTCACTGCGTTCGCCTGGCCC
CGCCGGCCCAGGAACCGACTCCAAAGGATTAGCCCCATGAGCATGTTGAAAGACCCTTCGCAGAAATACCGC
CCATTCCCGGCCATCAATCTGCCCGACCGCACCTGGCCGTCGAAGACCATCACCGAGGTGCCGATCTGGTGC
AGTTCCGACCTGCGCGACGGCAACCAGTCGCTGATCGAACCGATGGACGCGGCGAAGAAGATGCGGTTCTTC
AAGACCCTGGTGCAGGTCGGCCTGAAGCAGATCGAAGTGGCCTTCCCGTCGGCTTCCGATACCGACTTCAAC
TTCGTCCGCGAGTTGATCGAAGGCAACCACATCCCGGACGACGTCACCATCCAGGTGCTGACCCAGGCCCGC
GAAGACCTGATCACCCGCACCTTCGAATCCCTGCGCGGGGCGAAGAAGGCCATCGTCCACGTCTACAACGCC
ACCGCCCCGTCGTTCCGCCGCATCGTCTTCAACCAGGACAAGCAGGGCGTCGTGGACATCGCCACCAACGCC
GCGAAACTGATCAAGAAGCTGGCCGCCGAGCAGCCGGATACCCAGTGGTCGTTCCAGTACTCGCCGGAAATC
TTCAGCTCTACCGAGCTGGAGTTCTCGGTGGAGGTCTGCAACGCGGTGATCGACGTCTGGCAACCGACCCCG
GACAACAAGATCATCCTCAACCTGCCGGCCACCGTGGAGTGCGCCACGCCGAACGTCTACGCCGACCAGATC
GAATGGTTCGGCCGCCACGTCGACAAGCGTGACAGCGTGATCATCAGCCTGCACACCCACAACGACCGAGGC
ACCGGCGTGGCCGCCACCGAGCTGGGCCTGATGGCCGGCGCCGACCGCGTCGAAGGTTGCCTGTTCGGCAAC
GGCGAGCGCACCGGCAACGTCGACCTGGTGACCCTGGCCTTGAACATGTACACCCAGGGCCTGCATCCGCAA
CTGGACTTCTCCGACATCGACGCGGTGCGCAAGGTGGTCGAAGAGTGCAACCAGTTGCCGGTCCACCCGCGC
CATCCATATGTCGGCGACCTGGTCCATACCGCTTTCTCCGGCTCGCACCAGGATGCCATTCGCAAGGGCTTC
GCCCAGCAGAAGGAAGACGCGATCTGGGAAGTGCCGTACCTGCCGATCGATCCGGCCGACATCGGCCGCGAC
TACGAAGCGGTGATCCGGGTCAACAGCCAGTCCGGCAAGGGCGGCATCACCTTCCTGCTCGAACAGGAGTAC
GGCATCAGCCTGCCGCGGCGCATGCAGATCGAGTTCAGCCAGGTAGTGCAAGGCGAGACCGACCGTCTCGGC
CTGGAAATGACCGCACAACAGATCTACTCGCTGCTGGAAAACGAATACCTCAAGGCGACCTCGCCCTACGTC
CTCGCCAGCCATCGCCTGCAGGAAGAGAACGGCACCAGCGCCGTCGACCTGGAAGTCTCCTTCGACGGCGAG
AAGCAGCACTGGCGCGGGATCGGCAAGGGACCGCTGGAAGCCTTGGTGGCCGCCCTGCCGGTCAAGGCGGAA
ATCATGGACTACCATGAGCACGCCATCGGCGCCGGCGCCAATGCCAAGGCCGCGGCCTATATCGAGATCCGC
CTGGAGGGCCAGCGCCCGCTGCACGGCATCGGCATCGACGAGAACATCACCACCGCCAGCTTCCGCGCCCTG
TTCAGCGCGCTGAACCGCGCGGTGACCCAGGCCGAGGCCAAGGCGGCCTGA
Translation:
MPGQPATAWLSDASLHLVTAFAWPRRPRNRLQRISPMSMLKDPSQKYRPFPAINLPDRTWPSKTITEVPIWC
SSDLRDGNQSLIEPMDAAKKMRFFKTLVQVGLKQIEVAFPSASDTDFNFVRELIEGNHIPDDVTIQVLTQAR
EDLITRTFESLRGAKKAIVHVYNATAPSFRRIVFNQDKQGVVDIATNAAKLIKKLAAEQPDTQWSFQYSPEI
FSSTELEFSVEVCNAVIDVWQPTPDNKIILNLPATVECATPNVYADQIEWFGRHVDKRDSVIISLHTHNDRG
TGVAATELGLMAGADRVEGCLFGNGERTGNVDLVTLALNMYTQGLHPQLDFSDIDAVRKVVEECNQLPVHPR
HPYVGDLVHTAFSGSHQDAIRKGFAQQKEDAIWEVPYLPIDPADIGRDYEAVIRVNSQSGKGGITFLLEQEY
GISLPRRMQIEFSQVVQGETDRLGLEMTAQQIYSLLENEYLKATSPYVLASHRLQEENGTSAVDLEVSFDGE
KQHWRGIGKGPLEALVAALPVKAEIMDYHEHAIGAGANAKAAAYIEIRLEGQRPLHGIGIDENITTASFRAL
FSALNRAVTQAEAKAA*
AnnotationID:505GeneID:496
AnnotatorUID: diggins
Modification Date/Time: 2005-04-24 10:10:39
Gene Name:leuA
Confidence Code:2
GeneProduct: 2-isopropylmalate synthase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:KOG2367,COG0119
Secondary Functional Category(ies):
EC Number:2.3.3.13
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66650.1|AE016292_10 (AE016777) 2-isopropylmalate synthase [Pseudomonas putida KT2440]
          Length = 557

 Score =  933 bits (2412), Expect = 0.0
 Identities = 456/557 (81%), Positives = 507/557 (91%), Gaps = 1/557 (0%)

>emb|CAD17473.1| (AL646078) PROBABLE 2-ISOPROPYLMALATE SYNTHASE PROTEIN [Ralstonia
           solanacearum]
          Length = 576

 Score =  725 bits (1871), Expect = 0.0
 Identities = 355/559 (63%), Positives = 434/559 (77%), Gaps = 7/559 (1%)






 
Structural Features:
KOG2367, Alpha-isopropylmalate synthase/homocitrate synthase 
[Amino acid transport and metabolism]
CD-Length = 560 residues,  94.5% aligned
Score =  484 bits (1248), Expect = 2e-137
Identities = 185/555 (33%), Positives = 277/555 (49%), Gaps = 34/555 (6%)

COG0119, LeuA, Isopropylmalate/homocitrate/citramalate synthases 
[Amino acid transport and metabolism]
CD-Length = 409 residues,  99.5% aligned
Score =  340 bits (874), Expect = 6e-94
Identities = 132/437 (30%), Positives = 190/437 (43%), Gaps = 36/437 (8%)







   
Genomic Context:






   
Comment:


















    ReferenceID:14147




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18137




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31766




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:496_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:496
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
861
                           PA3792_tr
                           translation of PA3792
                           592
                           99.66
                           99.83
                           0
                           3058.0
     

     
     
35984
                           gb|AAG07179.1|AE004798_2
                           (AE004798) 2-isopropylmalate synthase [Pseudomonas aeruginosa PAO1]
                           592
                           99.49
                           99.83
                           0
                           3055.0
     

     
     
35986
                           gb|AAN66650.1|AE016292_10
                           (AE016777) 2-isopropylmalate synthase [Pseudomonas putida KT2440]
                           593
                           76.89
                           85.49
                           37
                           2414.0
     

     
     
35985
                           gb|AAO54965.1|
                           (AE016861) 2-isopropylmalate synthase [Pseudomonas syringae pv. tomato str. DC3000]
                           607
                           75.45
                           83.85
                           37
                           2423.0
     

     
     
35987
                           emb|CAD17473.1|
                           (AL646078) PROBABLE 2-ISOPROPYLMALATE SYNTHASE PROTEIN [Ralstonia solanacearum]
                           616
                           57.95
                           70.77
                           64
                           1880.0
     

     






      

          
GC ORFID: 43498How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3792Glimmer Score: 

          
Start: 3678285Stop: 3680063

          
Length: 1779


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3792 translation of PA3792Homolog Bit Score 1182.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4856 (ORF: BLASTX 939.0), t_PA3792 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGCCTGGTCAGCCCGCGACTGCGTGGCTGAGCGACGCTTCTCTTCATCTCGTCACTGCGTTCGCCTGGCCC 
CGCCGGCCCAGGAACCGACTCCAAAGGATTAGCCCCATGAGCATGTTGAAAGACCCTTCGCAGAAATACCGC 
CCATTCCCGGCCATCAATCTGCCCGACCGCACCTGGCCGTCGAAGACCATCACCGAGGTGCCGATCTGGTGC 
AGTTCCGACCTGCGCGACGGCAACCAGTCGCTGATCGAACCGATGGACGCGGCGAAGAAGATGCGGTTCTTC 
AAGACCCTGGTGCAGGTCGGCCTGAAGCAGATCGAAGTGGCCTTCCCGTCGGCTTCCGATACCGACTTCAAC 
TTCGTCCGCGAGTTGATCGAAGGCAACCACATCCCGGACGACGTCACCATCCAGGTGCTGACCCAGGCCCGC 
GAAGACCTGATCACCCGCACCTTCGAATCCCTGCGCGGGGCGAAGAAGGCCATCGTCCACGTCTACAACGCC 
ACCGCCCCGTCGTTCCGCCGCATCGTCTTCAACCAGGACAAGCAGGGCGTCGTGGACATCGCCACCAACGCC 
GCGAAACTGATCAAGAAGCTGGCCGCCGAGCAGCCGGATACCCAGTGGTCGTTCCAGTACTCGCCGGAAATC 
TTCAGCTCTACCGAGCTGGAGTTCTCGGTGGAGGTCTGCAACGCGGTGATCGACGTCTGGCAACCGACCCCG 
GACAACAAGATCATCCTCAACCTGCCGGCCACCGTGGAGTGCGCCACGCCGAACGTCTACGCCGACCAGATC 
GAATGGTTCGGCCGCCACGTCGACAAGCGTGACAGCGTGATCATCAGCCTGCACACCCACAACGACCGAGGC 
ACCGGCGTGGCCGCCACCGAGCTGGGCCTGATGGCCGGCGCCGACCGCGTCGAAGGTTGCCTGTTCGGCAAC 
GGCGAGCGCACCGGCAACGTCGACCTGGTGACCCTGGCCTTGAACATGTACACCCAGGGCCTGCATCCGCAA 
CTGGACTTCTCCGACATCGACGCGGTGCGCAAGGTGGTCGAAGAGTGCAACCAGTTGCCGGTCCACCCGCGC 
CATCCATATGTCGGCGACCTGGTCCATACCGCTTTCTCCGGCTCGCACCAGGATGCCATTCGCAAGGGCTTC 
GCCCAGCAGAAGGAAGACGCGATCTGGGAAGTGCCGTACCTGCCGATCGATCCGGCCGACATCGGCCGCGAC 
TACGAAGCGGTGATCCGGGTCAACAGCCAGTCCGGCAAGGGCGGCATCACCTTCCTGCTCGAACAGGAGTAC 
GGCATCAGCCTGCCGCGGCGCATGCAGATCGAGTTCAGCCAGGTAGTGCAAGGCGAGACCGACCGTCTCGGC 
CTGGAAATGACCGCACAACAGATCTACTCGCTGCTGGAAAACGAATACCTCAAGGCGACCTCGCCCTACGTC 
CTCGCCAGCCATCGCCTGCAGGAAGAGAACGGCACCAGCGCCGTCGACCTGGAAGTCTCCTTCGACGGCGAG 
AAGCAGCACTGGCGCGGGATCGGCAAGGGACCGCTGGAAGCCTTGGTGGCCGCCCTGCCGGTCAAGGCGGAA 
ATCATGGACTACCATGAGCACGCCATCGGCGCCGGCGCCAATGCCAAGGCCGCGGCCTATATCGAGATCCGC 
CTGGAGGGCCAGCGCCCGCTGCACGGCATCGGCATCGACGAGAACATCACCACCGCCAGCTTCCGCGCCCTG 
TTCAGCGCGCTGAACCGCGCGGTGACCCAGGCCGAGGCCAAGGCGGCCTGA