ViewGene
Locus Name:
GeneID:
Location:

GeneID:4955
TrivialName: PA14_05860
AnnotatorUID: danlee
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_05860
PAO1 Ortholog Locus:
Sequence Length:405
Protein Length:134
Start: 518460
Stop: 518864
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: Matches last 134 amino acids (last 75%) of PA0449: gb|AAG03838.1|AE004482_5 (AE004482) hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 134/134 (100%), Positives = 134/134 (100%)
Sequence:
ATGAATTTCCACACCCGCAAGTGGGTCAAGCCAGAAGATCTCAACCCCAACGGCACCCTGTTCGGCGGCAGC
CTGCTGCGCTGGATCGACGAAGAAGCGGCGATCTACGCGATCATCCAGCTGGGCAACCAGCGCGTGGTCACC
AAGTACATTTCCGAGATCAACTTCGTCAGCTCGGCGCGCCAGGGCGACATCATCGAGCTGGGCATCACCGCC
ACCGACTTCGGTCGTACCTCGCTGACCATGACCTGCCACGTGCGCAACAAGATCACCCGCAAGAGCATCCTC
ACCATCGACAAGATGGTCTTCGTCAACCTCGGCCCGGACGGCCTGCCGGCGCCCCACGGCAAGACCGAGATC
ACCTACATCAAGGACCAGTTCAAGGAAGACGAAGTCCTGCCCTGA
Translation:
MNFHTRKWVKPEDLNPNGTLFGGSLLRWIDEEAAIYAIIQLGNQRVVTKYISEINFVSSARQGDIIELGITA
TDFGRTSLTMTCHVRNKITRKSILTIDKMVFVNLGPDGLPAPHGKTEITYIKDQFKEDEVLP*
AnnotationID:4960GeneID:4955
AnnotatorUID: danlee
Modification Date/Time: 2006-02-08 16:04:14
Gene Name:
Confidence Code:3
GeneProduct:putative long-chain acyl-CoA thioester hydrolase family protein
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1607
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gi|9946308|gb|AAG03838.1|  hypothetical protein PA0449 [Pseudomonas aeruginosa PAO1]
          Length=179

 Score =  273 bits (699),  Expect = 9e-73
 Identities = 134/134 (100%), Positives = 134/134 (100%), Gaps = 0/134 (0%)

>gb|AAN70542.1|AE016696_7 (AE016792) long-chain acyl-CoA thioester hydrolase family protein
           [Pseudomonas putida KT2440]
          Length = 133

 Score =  235 bits (599), Expect = 4e-62
 Identities = 112/132 (84%), Positives = 123/132 (93%)

>gb|AAO58494.1| (AE016874) 4-hydroxybenzoyl-CoA thioesterase domain protein
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 131

 Score =  233 bits (595), Expect = 1e-61
 Identities = 111/131 (84%), Positives = 123/131 (93%)

>gb|AAN54547.1|AE015593_6 (AE015593) cytosolic long-chain acyl-CoA thioester hydrolase family
           protein [Shewanella oneidensis MR-1]
          Length = 122

 Score =  141 bits (355), Expect = 7e-34
 Identities = 65/116 (56%), Positives = 86/116 (74%)






 
Structural Features:
COG1607, Acyl-CoA_hydrolase_[Lipid_metabolism], Acyl-CoA hydrolase 
[Lipid metabolism]
CD-Length = 157 residues,  77.7% aligned
Score =  114 bits (288), Expect = 3e-27
Identities = 43/129 (33%), Positives = 56/129 (43%), Gaps = 8/129 (6%)







   
Genomic Context:






   
Comment:
Confirmed frame-shift results in truncation of first 45 aa of PAO1 PA0449.


















    ReferenceID:9910




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:27051




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33768




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4955_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:4955
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
191100
                           gb|AAN70542.1|AE016696_7
                           (AE016792) long-chain acyl-CoA thioester hydrolase family protein [Pseudomonas putida KT2440]
                           134
                           83.58
                           91.79
                           1
                           596.0
     

     
     
191101
                           gb|AAO58494.1|
                           (AE016874) 4-hydroxybenzoyl-CoA thioesterase domain protein [Pseudomonas syringae pv. tomato str. DC3000]
                           134
                           82.83
                           91.79
                           3
                           595.0
     

     
     
7361
                           PA0449_tr
                           translation of PA0449
                           179
                           74.86
                           74.86
                           45
                           699.0
     

     
     
191099
                           gb|AAG03838.1|AE004482_5
                           (AE004482) hypothetical protein PA0449 [Pseudomonas aeruginosa PAO1]
                           179
                           74.86
                           74.86
                           45
                           699.0
     

     
     
191102
                           gb|AAN54547.1|AE015593_6
                           (AE015593) cytosolic long-chain acyl-CoA thioester hydrolase family protein [Shewanella oneidensis MR-1]
                           134
                           48.50
                           64.17
                           12
                           345.0
     

     






      

          
GC ORFID: 47957How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: psyr_15may02_Scaffold2_revised_gene3336Glimmer Score: 

          
Start: 6319802Stop: 6320206

          
Length: 405


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: psyr_15may02_Scaffold2_revised_gene3336 related to: gi|15595646|ref|NP_249140.1| (NC_002516) hypothetical protein [Pseudomonas aeruginosa] pir||F83589 hypothetical protein PA0449 [imported] - Pseudomonas aeruginosa (strain PAO1) gb|AAG03838.1|AE004482_5 (Homolog Bit Score 233.0

          
Other Homologs:  t_PA0449 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAATTTCCACACCCGCAAGTGGGTCAAGCCAGAAGATCTCAACCCCAACGGCACCCTGTTCGGCGGCAGC 
CTGCTGCGCTGGATCGACGAAGAAGCGGCGATCTACGCGATCATCCAGCTGGGCAACCAGCGCGTGGTCACC 
AAGTACATTTCCGAGATCAACTTCGTCAGCTCGGCGCGCCAGGGCGACATCATCGAGCTGGGCATCACCGCC 
ACCGACTTCGGTCGTACCTCGCTGACCATGACCTGCCACGTGCGCAACAAGATCACCCGCAAGAGCATCCTC 
ACCATCGACAAGATGGTCTTCGTCAACCTCGGCCCGGACGGCCTGCCGGCGCCCCACGGCAAGACCGAGATC 
ACCTACATCAAGGACCAGTTCAAGGAAGACGAAGTCCTGCCCTGA