ViewGene
Locus Name:
GeneID:
Location:

GeneID:4904
TrivialName: PA14_35130
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_35130
PAO1 Ortholog Locus:PA2277
Sequence Length:351
Protein Length:116
Start: 3126334
Stop: 3125984
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05665.1|AE004653_8 (AE004653) ArsR protein [Pseudomonas aeruginosa PAO1]
Identities = 116/116 (100%), Positives = 116/116 (100%)
Sequence:
ATGCCCAGCCCCGCCGAAGTGTTCAAGTGCCTGGCCGACGAGACGCGCGTTCGCGCCACCCTGCTGATCGTC
GACCAGGGCGAACTGTGCGTCTGCGAACTGATGTGCGCCCTCGCCGACAGCCAGCCGAAGATCAGCCGCCAC
CTGGCCCAGTTGCGCAGTGCCGGGCTGCTCCTCGACCGTCGCCAGGGCCAGTGGGTGTATTACCGCCTGAAC
CCTGCGCTGCCGGCCTGGATCCACGAAGTCCTGCAAGTGACCCTGCGGGCCAACGGCGACTGGCTGCAAGCC
GACGCCGCGCGCCTGCGCGACATGGACGGCCGCCCGCAGCGCGCCTCCGCCTGCTGCCAATAG
Translation:
MPSPAEVFKCLADETRVRATLLIVDQGELCVCELMCALADSQPKISRHLAQLRSAGLLLDRRQGQWVYYRLN
PALPAWIHEVLQVTLRANGDWLQADAARLRDMDGRPQRASACCQ*
AnnotationID:4909GeneID:4904
AnnotatorUID: diggins
Modification Date/Time: 2005-03-04 09:09:57
Gene Name:arsR
Confidence Code:1
GeneProduct:arsenic resistance transcriptional regulator
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(25) Transcriptional regulators
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:smart00418,pfam01022
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN68326.1|AE016466_11 (AE016784) arsenic resistance transcriptional regulator
           [Pseudomonas putida KT2440]
          Length = 115

 Score =  183 bits (464), Expect = 2e-46
 Identities = 86/115 (74%), Positives = 97/115 (84%)

>gb|AAN53613.1|AE015500_12 (AE015500) arsenical resistence operon repressor [Shewanella
           oneidensis MR-1]
          Length = 114

 Score =  136 bits (343), Expect = 2e-32
 Identities = 66/112 (58%), Positives = 80/112 (71%)

>gb|AAP19194.1| (AE016992) transcriptional repressor of chromosomal ars operon
          [Shigella flexneri 2a str. 2457T]
          Length = 114








 
Structural Features:
smart00418, HTH_ARSR, helix_turn_helix, Arsenical Resistance Operon Repressor
CD-Length = 79 residues, 100.0% aligned
Score = 77.6 bits (191), Expect = 4e-16
Identities = 34/80 (42%), Positives = 49/80 (61%), Gaps = 2/80 (2%)

pfam01022, HTH_5, Bacterial regulatory protein, arsR family.
CD-Length = 80 residues, 100.0% aligned
Score = 76.9 bits (189), Expect = 5e-16
Identities = 33/80 (41%), Positives = 47/80 (58%), Gaps = 2/80 (2%)







   
Genomic Context:






   
Comment:


















    ReferenceID:9808




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:26949




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32504




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4904_cdd.html


    		




 
URL:

















    ReferenceID:34453




  
Author/Investigator(s):
        Cai J, Salmon K, DuBow MS.





  
Title:
        A chromosomal ars operon homologue of Pseudomonas aeruginosa confers increased resistance to arsenic and antimony in Escherichia coli.




  
PubMed:
        9802012


MedLine:
        




    
Source:
Microbiology. 1998 Oct;144 ( Pt 10):2705-13.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9802012

















    ReferenceID:34454




  
Author/Investigator(s):
        Diorio C, Cai J, Marmor J, Shinder R, DuBow MS.





  
Title:
        An Escherichia coli chromosomal ars operon homolog is functional in arsenic detoxification and is conserved in gram-negative bacteria.




  
PubMed:
        7721697


MedLine:
        




    
Source:
J Bacteriol. 1995 Apr;177(8):2050-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7721697









           
Homologs By Global Alignment 

           

       



     
Gene ID:4904
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
7309
                           PA2277_tr
                           translation of PA2277
                           116
                           99.99
                           99.99
                           0
                           613.0
     

     
     
190288
                           gb|AAG05665.1|AE004653_8
                           (AE004653) ArsR protein [Pseudomonas aeruginosa PAO1]
                           116
                           99.99
                           99.99
                           0
                           613.0
     

     
     
190289
                           gb|AAC69642.1|
                           (AF010234) ArsR [Pseudomonas aeruginosa]
                           126
                           79.36
                           80.15
                           18
                           479.0
     

     
     
190290
                           gb|AAN68326.1|AE016466_11
                           (AE016784) arsenic resistance transcriptional regulator [Pseudomonas putida KT2440]
                           116
                           74.13
                           83.62
                           1
                           464.0
     

     
     
190291
                           emb|CAC18652.1|
                           (AJ271973) arsenic resistance operon regulator [Pseudomonas putida]
                           119
                           68.06
                           76.47
                           4
                           427.0
     

     






      

          
GC ORFID: 47906How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2277Glimmer Score: 

          
Start: 1827582Stop: 1827932

          
Length: 351


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA2277 translation of PA2277Homolog Bit Score 240.0

          
Other Homologs:  psyr_15may02_Scaffold10_revised_gene1594 (ORF: BLASTX 163.0), t_PA2277 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCCAGCCCCGCCGAAGTGTTCAAGTGCCTGGCCGACGAGACGCGCGTTCGCGCCACCCTGCTGATCGTC 
GACCAGGGCGAACTGTGCGTCTGCGAACTGATGTGCGCCCTCGCCGACAGCCAGCCGAAGATCAGCCGCCAC 
CTGGCCCAGTTGCGCAGTGCCGGGCTGCTCCTCGACCGTCGCCAGGGCCAGTGGGTGTATTACCGCCTGAAC 
CCTGCGCTGCCGGCCTGGATCCACGAAGTCCTGCAAGTGACCCTGCGGGCCAACGGCGACTGGCTGCAAGCC 
GACGCCGCGCGCCTGCGCGACATGGACGGCCGCCCGCAGCGCGCCTCCGCCTGCTGCCAATAG