ViewGene
Locus Name:
GeneID:
Location:

GeneID:489
TrivialName: PA14_21050
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_21050
PAO1 Ortholog Locus:PA3324
Sequence Length:1779
Protein Length:592
Start: 1820782
Stop: 1819004
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06712.1|AE004754_8 (AE004754) probable short-chain dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 585/592 (98%), Positives = 586/592 (98%)
Sequence:
ATGAACGCGCTCCTCGCCCTCCCCGACCTGCAGCCGGAACGGCTGTTCGTGCAGTCCGGCGACGTGCGCCTG
GCGGTTCATTGCTGGGGCGCGCCGGACAACGACAAGCCGACGCTGCTGATGGTGCATGGCTACCCGGACAAC
CACGAGACCTGGCTGCCGCTGATCCGCCAACTGGCTGGGCGCTACCGCATCGTCGCCTACGACGTGCGCGGC
GCCGGCGCTTCGGACAAGCCACGCTGGAGCCGCGACTACCACCTGCAACGGCTCAGCGAAGACCTGCAGGCG
GTGATTCGCGCCACCAGCCCGGATCGCCCGGTGCACCTGCTGGCCCATGACTGGGGCTCGATCCAGACCTGG
GAAAGCGTCACCGATCCGCAGTGCCGCCCGCTGATCGCGTCCTATACCTCGATCTCCGGGCCCTGCCTGGAC
CATGTCGGCTTCTGGATGCGCGAACACCTCAGGCAGCGCAGTCCGAAGGCCCTGAAAGCGGTATTCGGCCAG
TTGCTCCACTCCTGGTACATCGCCTTCTTCCATACCCCCGTCGTACCCGAGCTGCTCTGGAGCGTCGGCCTG
GCCCGGCTCTGGCCGCAGTTCTTGAAGCGCGCCGAAGGCGTCCGCCATCCACAGGTCAACCCGACCCAGGCC
AGCGACGGCCGGCACGGGGTCAAGCTGTACCGCGGCAACTTCATCCGCAGCCTGTTCCGTCCGCGCAAGCGC
CACACCGAAGTGCCGGTGCAACTGATCGTACCGACCCGCGACCGTTACGTCGGCGCCCAGCTGTTCCAGCAC
CTCAGCCTGTGGGCGCCACGCCTGTGGCGGCGCGAGGCGAGCGTCGGCCATTGGCAACTGCTGGCCGAGCCG
GAGCAACTGGCCGGCTGGCTTGGCGAGTTCATCGACGCCCAGGAAACCGGCGAGTCGCCGCCGGCCCTGCAA
CGCGCACAGGTCCGCCCCGACGCGCGCTCGATGAGCGGCAAGCTGGTGGTGGTCACCGGCGCCGGCGGCGGC
ATCGGCCGTTCGACCCTGCTCAGCTTCGCCGAGCGCGGCGCCAGCCTGCTCGCCGCCGATCTCGACCTGGAA
GCCGCCGAACGCAGCGCCGAACTGGCCCGCGCCCTTGGCGCCACGGCCCATGCCTACCAGGTCGACGTCGGC
GATACCCAGGCCATGGAGCGCTTCGCCGAGTGGGTCCGCGACACCCTCGGAGTCCCGGATGTGGTGGTCAGC
AATGCCGGCATCGGCATGGCCGGACCGATGCTCGACACCTCGCCCGCGGAATGGGAGCGCCTGCTGCGGGTC
AACCTGTGGAGCGTGATCGACGGCTGCCGCCTGTTCGGCCGGCAGATGATCGCGGCGAACAAGCCGGGGCAC
CTGGTCAATGTCGCCTCCGGCGTGGCCTTCGCGCCGTCGCGCAACTACCCCGCCTACGCCACCAGCAAGGCC
GCGGTACTGATGCTCAGCGAATGCCTGCGGGCGGAACTGGCCGGACGTTCGATCGGAGTCACCGCGGTGTGC
CCGGGCTTCGTCGATACCGGCATCGTCCAGGCCACCCGCTTCGTCGGCATGGACGCCGAACGCCAGGCGCGG
CGCCAGGCGAAGATCCAGCGCTTCTACAAGCGACGCCGGCTCAGCCCGGACACCGTTGGGGAAAAGCTGGTG
CGCGCCGTGGAGCGCAACAAGGCGGTGGTCTCGGTGGGCAGCGAGGTGCACCTCGGCGCCCTGCAATGGCGC
TTCGCGCCCTGGGCCACGCGGTTTCTCGCGCGCTTCGACCTGACTTCCTGA
Translation:
MNALLALPDLQPERLFVQSGDVRLAVHCWGAPDNDKPTLLMVHGYPDNHETWLPLIRQLAGRYRIVAYDVRG
AGASDKPRWSRDYHLQRLSEDLQAVIRATSPDRPVHLLAHDWGSIQTWESVTDPQCRPLIASYTSISGPCLD
HVGFWMREHLRQRSPKALKAVFGQLLHSWYIAFFHTPVVPELLWSVGLARLWPQFLKRAEGVRHPQVNPTQA
SDGRHGVKLYRGNFIRSLFRPRKRHTEVPVQLIVPTRDRYVGAQLFQHLSLWAPRLWRREASVGHWQLLAEP
EQLAGWLGEFIDAQETGESPPALQRAQVRPDARSMSGKLVVVTGAGGGIGRSTLLSFAERGASLLAADLDLE
AAERSAELARALGATAHAYQVDVGDTQAMERFAEWVRDTLGVPDVVVSNAGIGMAGPMLDTSPAEWERLLRV
NLWSVIDGCRLFGRQMIAANKPGHLVNVASGVAFAPSRNYPAYATSKAAVLMLSECLRAELAGRSIGVTAVC
PGFVDTGIVQATRFVGMDAERQARRQAKIQRFYKRRRLSPDTVGEKLVRAVERNKAVVSVGSEVHLGALQWR
FAPWATRFLARFDLTS*
AnnotationID:498GeneID:489
AnnotatorUID: diggins
Modification Date/Time: 2005-04-15 08:08:41
Gene Name:
Confidence Code:3
GeneProduct:putative short-chain dehydrogenase
Cell Localization:()
Synonyms:PA3324
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(20) Putative enzymes
Alternate Gene Product Name:putative oxidoreductase
Functional Category Confidence Code:5
COGs:pfam00106,COG0300,COG1028,COG4221
Secondary Functional Category(ies):biosynthesiis of cofactors, prosthetic groups and carriers
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAA22226.1| (AL939118) putative oxidoreductase [Streptomyces coelicolor A3(2)]
          Length = 585

 Score =  521 bits (1342), Expect = e-147
 Identities = 285/576 (49%), Positives = 360/576 (62%), Gaps = 12/576 (2%)

>emb|CAB11381.1| (Z98741) oxidoreductase [Mycobacterium leprae]
          Length = 596

 Score =  404 bits (1038), Expect = e-112
 Identities = 237/586 (40%), Positives = 334/586 (56%), Gaps = 21/586 (3%)






 
Structural Features:
pfam00106, adh_short, short chain dehydrogenase.
CD-Length = 247 residues,  98.4% aligned
Score =  182 bits (464), Expect = 7e-47
Identities = 78/244 (31%), Positives = 115/244 (47%), Gaps = 2/244 (0%)

COG0300, DltE, Short-chain dehydrogenases of various substrate specificities
CD-Length = 265 residues,  92.5% aligned
Score =  167 bits (425), Expect = 3e-42
Identities = 80/258 (31%), Positives = 118/258 (45%), Gaps = 14/258 (5%)

COG1028, FabG, Dehydrogenases with different specificities
CD-Length = 251 residues,  86.1% aligned
Score =  148 bits (375), Expect = 1e-36
Identities = 68/220 (30%), Positives = 108/220 (49%), Gaps = 9/220 (4%)

COG4221, Short-chain alcohol dehydrogenase of unknown 
specificity
CD-Length = 246 residues,  93.9% aligned
Score =  138 bits (350), Expect = 1e-33
Identities = 76/243 (31%), Positives = 121/243 (49%), Gaps = 14/243 (5%)






   
Genomic Context:






   
Comment:


















    ReferenceID:981




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18123




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32239




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:489_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:489
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
854
                           PA3324_tr
                           translation of PA3324
                           592
                           98.81
                           98.98
                           0
                           3074.0
     

     
     
35584
                           gb|AAG06712.1|AE004754_8
                           (AE004754) probable short-chain dehydrogenase [Pseudomonas aeruginosa PAO1]
                           592
                           98.81
                           98.98
                           0
                           3074.0
     

     
     
35585
                           emb|CAA22226.1|
                           (AL939118) putative oxidoreductase [Streptomyces coelicolor A3(2)]
                           597
                           48.07
                           60.97
                           17
                           1347.0
     

     
     
35586
                           dbj|BAC71964.1|
                           (AP005038) putative oxidoreductase [Streptomyces avermitilis MA-4680]
                           598
                           46.48
                           62.20
                           20
                           1336.0
     

     
     
35587
                           emb|CAB11381.1|
                           (Z98741) oxidoreductase [Mycobacterium leprae]
                           613
                           39.47
                           55.79
                           38
                           1059.0
     

     






      

          
GC ORFID: 43491How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3324Glimmer Score: 

          
Start: 3133150Stop: 3134928

          
Length: 1779


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3324 translation of PA3324Homolog Bit Score 1188.0

          
Other Homologs:  t_PA3324 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACGCGCTCCTCGCCCTCCCCGACCTGCAGCCGGAACGGCTGTTCGTGCAGTCCGGCGACGTGCGCCTG 
GCGGTTCATTGCTGGGGCGCGCCGGACAACGACAAGCCGACGCTGCTGATGGTGCATGGCTACCCGGACAAC 
CACGAGACCTGGCTGCCGCTGATCCGCCAACTGGCTGGGCGCTACCGCATCGTCGCCTACGACGTGCGCGGC 
GCCGGCGCTTCGGACAAGCCACGCTGGAGCCGCGACTACCACCTGCAACGGCTCAGCGAAGACCTGCAGGCG 
GTGATTCGCGCCACCAGCCCGGATCGCCCGGTGCACCTGCTGGCCCATGACTGGGGCTCGATCCAGACCTGG 
GAAAGCGTCACCGATCCGCAGTGCCGCCCGCTGATCGCGTCCTATACCTCGATCTCCGGGCCCTGCCTGGAC 
CATGTCGGCTTCTGGATGCGCGAACACCTCAGGCAGCGCAGTCCGAAGGCCCTGAAAGCGGTATTCGGCCAG 
TTGCTCCACTCCTGGTACATCGCCTTCTTCCATACCCCCGTCGTACCCGAGCTGCTCTGGAGCGTCGGCCTG 
GCCCGGCTCTGGCCGCAGTTCTTGAAGCGCGCCGAAGGCGTCCGCCATCCACAGGTCAACCCGACCCAGGCC 
AGCGACGGCCGGCACGGGGTCAAGCTGTACCGCGGCAACTTCATCCGCAGCCTGTTCCGTCCGCGCAAGCGC 
CACACCGAAGTGCCGGTGCAACTGATCGTACCGACCCGCGACCGTTACGTCGGCGCCCAGCTGTTCCAGCAC 
CTCAGCCTGTGGGCGCCACGCCTGTGGCGGCGCGAGGCGAGCGTCGGCCATTGGCAACTGCTGGCCGAGCCG 
GAGCAACTGGCCGGCTGGCTTGGCGAGTTCATCGACGCCCAGGAAACCGGCGAGTCGCCGCCGGCCCTGCAA 
CGCGCACAGGTCCGCCCCGACGCGCGCTCGATGAGCGGCAAGCTGGTGGTGGTCACCGGCGCCGGCGGCGGC 
ATCGGCCGTTCGACCCTGCTCAGCTTCGCCGAGCGCGGCGCCAGCCTGCTCGCCGCCGATCTCGACCTGGAA 
GCCGCCGAACGCAGCGCCGAACTGGCCCGCGCCCTTGGCGCCACGGCCCATGCCTACCAGGTCGACGTCGGC 
GATACCCAGGCCATGGAGCGCTTCGCCGAGTGGGTCCGCGACACCCTCGGAGTCCCGGATGTGGTGGTCAGC 
AATGCCGGCATCGGCATGGCCGGACCGATGCTCGACACCTCGCCCGCGGAATGGGAGCGCCTGCTGCGGGTC 
AACCTGTGGAGCGTGATCGACGGCTGCCGCCTGTTCGGCCGGCAGATGATCGCGGCGAACAAGCCGGGGCAC 
CTGGTCAATGTCGCCTCCGGCGTGGCCTTCGCGCCGTCGCGCAACTACCCCGCCTACGCCACCAGCAAGGCC 
GCGGTACTGATGCTCAGCGAATGCCTGCGGGCGGAACTGGCCGGACGTTCGATCGGAGTCACCGCGGTGTGC 
CCGGGCTTCGTCGATACCGGCATCGTCCAGGCCACCCGCTTCGTCGGCATGGACGCCGAACGCCAGGCGCGG 
CGCCAGGCGAAGATCCAGCGCTTCTACAAGCGACGCCGGCTCAGCCCGGACACCGTTGGGGAAAAGCTGGTG 
CGCGCCGTGGAGCGCAACAAGGCGGTGGTCTCGGTGGGCAGCGAGGTGCACCTCGGCGCCCTGCAATGGCGC 
TTCGCGCCCTGGGCCACGCGGTTTCTCGCGCGCTTCGACCTGACTTCCTGA