ViewGene
Locus Name:
GeneID:
Location:

GeneID:488
TrivialName: PA14_73170
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_73170
PAO1 Ortholog Locus:PA5549
Sequence Length:1836
Protein Length:611
Start: 6518202
Stop: 6516367
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08934.1|AE004967_5 (AE004967) glucosamine--fructose-6-phosphate aminotransferase [Pseudomonas aeruginosa PAO1]
Identities = 609/611 (99%), Positives = 611/611 (100%)
Sequence:
ATGTGTGGAATCGTAGGTGCGATCGCCGAGCGCAACATCACCCCCATCCTCATCGAAGGCCTCAAGCGCCTC
GAGTATCGCGGCTACGACAGCGCCGGCGTGGCGGTCTTCGATAACGAAGGCAGGCTGCAGCGCTGCCGCCGG
GTCGGCAAGGTCGCCAGCCTGGAGGAAGGCCTGGCCGGTACGCCGCTGCTCGGCCGCCTGGGCATCGCCCAT
ACCCGCTGGGCGACCCACGGCGCGCCAACCGAAGGCAACGCCCACCCGCATTTCTCCAGCGACGAACTGGCG
GTGGTGCACAACGGCATCATCGAGAACCACGAACCTCTCCGTGAGCGGCTCAAGGGCCTGGGCTACGTGTTC
ACCTCGCAGACCGACACCGAGGTCATCGTCCACCTGCTGCATCACAAGCTGCAGTCCATCGGCGACCTGACC
CTGGCGCTGAAGGACGCGGTCAAGGAATTGCACGGGGCCTATGGCCTGGCAGTGATCAGCGCGGCGCAGCCG
GACCGCATCGTCGCCGCCCGCAGCGGCAGCCCGCTGGTGATCGGCCTCGGGCTTGGCGAGAACTTCCTCGCT
TCCGACCAACTGGCGCTGCGCCAGGTGACCGACCGCTTCATCTACCTGGAAGAAGGCGATATCGCCGAGATC
CGTCGCGACAGCGTGCGCCTGTGGGATGTCCAGGGCAACGACGTGCAGCGCGAGACCGTGCAGTACCACGAA
GGCGCCGAAGCCGCCGACAAGGGCGAGTACCGGCACTTCATGCTCAAGGAAATCCACGAGCAGCCCAGCGTC
GTGCAGCGCACCCTGGAAGGCCGCCTCGGGCAGAACCAGGTAATGGTGGAAAGCTTCGGCCCGCAGGCGGCG
GAGCTGTTCGCCAAGGTTCGCAACGTGCAGATCGTCGCCTGCGGTACCAGCTACCACGCCGGCATGGTCGCC
CGTTACTGGCTGGAAAGCCTGACCGGGATTCCCTGCCAGGTGGAGGTCGCCAGCGAGTTCCGCTACCGCAAG
GTCGCGGTGCAGCCGGATTGCCTGTTCGTCACCATCTCCCAGTCCGGTGAGACCGCCGACACCCTGGCTGCC
CTGCGCAACGCCAAGGAGCTGGGCTTCCTCTCCAGCGTGGCGATCTGCAACGTTGCCACCAGTTCGCTGGTG
CGCGAATCCGACCTGACCCTGCTGACCCAGGCCGGCCCGGAAATCGGCGTGGCCTCGACCAAGGCCTTCACC
ACCCAGTTGGTCGCCCTGCTGCTGCTGACCCTGGGCATCGGCCAGGTGCAGAAGCGCCTGGCCGATGGCGTC
GAAGCCGAGCTGGTGGACGAACTGCGTCGCCTGCCGACCCGGCTGGGCGAGGCGCTGGCGATGAACCGGACG
GTGGAGAAGGTTTCCGAGCTGTTCGCCGAGAAGCACCACACCCTGTTCCTCGGCCGTGGCGCGCAGTTCCCG
GTGGCCCTGGAAGGCGCGCTGAAGCTCAAGGAAATTTCCTATATCCATGCCGAAGCCTACCCGGCCGGCGAA
CTCAAGCATGGCCCGCTGGCGCTGGTGGACAGCGACATGCCGGTGGTTACCGTGGCGCCGAACAACGAGCTG
GTGGAGAAGCTCAAGTCGAACCTGCAGGAAGTCCGCGCCCGCGGCGGCGAACTGGTGGTGTTCGCCGACGAA
GGCGCGGGCATCGAGGCCGGCGAGGGCACCCATGTGGTGGGCATGCCGCACATCGGCGACGTGCTCTCGCCG
ATCCTCTACACCATCCCGTTGCAATTGCTCTCGTACCATGTCGCCGTGCTCAAGGGCACCGACGTCGACCAG
CCGCGCAACCTGGCCAAGTCGGTCACCGTCGAGTAA
Translation:
MCGIVGAIAERNITPILIEGLKRLEYRGYDSAGVAVFDNEGRLQRCRRVGKVASLEEGLAGTPLLGRLGIAH
TRWATHGAPTEGNAHPHFSSDELAVVHNGIIENHEPLRERLKGLGYVFTSQTDTEVIVHLLHHKLQSIGDLT
LALKDAVKELHGAYGLAVISAAQPDRIVAARSGSPLVIGLGLGENFLASDQLALRQVTDRFIYLEEGDIAEI
RRDSVRLWDVQGNDVQRETVQYHEGAEAADKGEYRHFMLKEIHEQPSVVQRTLEGRLGQNQVMVESFGPQAA
ELFAKVRNVQIVACGTSYHAGMVARYWLESLTGIPCQVEVASEFRYRKVAVQPDCLFVTISQSGETADTLAA
LRNAKELGFLSSVAICNVATSSLVRESDLTLLTQAGPEIGVASTKAFTTQLVALLLLTLGIGQVQKRLADGV
EAELVDELRRLPTRLGEALAMNRTVEKVSELFAEKHHTLFLGRGAQFPVALEGALKLKEISYIHAEAYPAGE
LKHGPLALVDSDMPVVTVAPNNELVEKLKSNLQEVRARGGELVVFADEGAGIEAGEGTHVVGMPHIGDVLSP
ILYTIPLQLLSYHVAVLKGTDVDQPRNLAKSVTVE*
AnnotationID:497GeneID:488
AnnotatorUID: diggins
Modification Date/Time: 2005-03-22 07:07:20
Gene Name:glmS
Confidence Code:2
GeneProduct:glucosamine--fructose-6-phosphate aminotransferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0449
Secondary Functional Category(ies):
EC Number:2.6.1.16
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO59008.1| (AE016876) glucosamine--fructose-6-phosphate aminotransferase,
           isomerizing [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 611

 Score = 1042 bits (2694), Expect = 0.0
 Identities = 527/611 (86%), Positives = 568/611 (92%)

>gb|AAN70973.1|AE016742_2 (AE016794) glucosamine--fructose-6-phosphate aminotransferase,
           isomerizing [Pseudomonas putida KT2440]
          Length = 611

 Score = 1033 bits (2671), Expect = 0.0
 Identities = 523/611 (85%), Positives = 564/611 (92%)

>gb|AAM87674.1|AE014014_8 (AE014014) L-glutamine:D-fructose-6-phosphate aminotransferase
           [Yersinia pestis KIM]
          Length = 609

 Score =  776 bits (2003), Expect = 0.0
 Identities = 384/611 (62%), Positives = 484/611 (79%), Gaps = 2/611 (0%)






 
Structural Features:
COG0449, GlmS, Glucosamine 6-phosphate synthetase, contains amidotransferase 
and phosphosugar isomerase domains 
[Cell envelope biogenesis, outer membrane]
CD-Length = 597 residues, 100.0% aligned
Score =  817 bits (2112), Expect = 0.0
Identities = 336/612 (54%), Positives = 428/612 (69%), Gaps = 16/612 (2%)








   
Genomic Context:






   
Comment:


















    ReferenceID:979




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18121




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33336




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:488_cdd.html


    		




 
URL:

















    ReferenceID:35590




  
Author/Investigator(s):
        Walker JE, Gay NJ, Saraste M, Eberle AN.





  
Title:
        DNA sequence around the Escherichia coli unc operon. Completion of the sequence of a 17 kilobase segment containing asnA, oriC, unc, glmS and phoS.




  
PubMed:
        6395859


MedLine:
        




    
Source:
Biochem J. 1984 Dec 15;224(3):799-815.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6395859

















    ReferenceID:35591




  
Author/Investigator(s):
        Zelder O, Beatrix B, Buckel W.





  
Title:
        Cloning, sequencing and expression in Escherichia coli of the gene encoding component S of the coenzyme B12-dependent glutamate mutase from Clostridium cochlearium.




  
PubMed:
        8013871


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1994 May 1;118(1-2):15-21.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8013871









           
Homologs By Global Alignment 

           

       



     
Gene ID:488
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
853
                           PA5549_tr
                           translation of PA5549
                           611
                           99.67
                           99.99
                           0
                           3081.0
     

     
     
35449
                           gb|AAG08934.1|AE004967_5
                           (AE004967) glucosamine--fructose-6-phosphate aminotransferase [Pseudomonas aeruginosa PAO1]
                           611
                           99.67
                           99.99
                           0
                           3081.0
     

     
     
35450
                           gb|AAO59008.1|
                           (AE016876) glucosamine--fructose-6-phosphate aminotransferase, isomerizing [Pseudomonas syringae pv. tomato str. DC3000]
                           611
                           86.25
                           92.96
                           0
                           2694.0
     

     
     
35451
                           gb|AAN70973.1|AE016742_2
                           (AE016794) glucosamine--fructose-6-phosphate aminotransferase, isomerizing [Pseudomonas putida KT2440]
                           611
                           85.59
                           92.30
                           0
                           2671.0
     

     
     
35460
                           gb|AAF93660.1|
                           (AE004135) glucosamine--fructose-6-phosphate aminotransferase (isomerizing) [Vibrio cholerae O1 biovar eltor str. N16961]
                           611
                           63.01
                           78.39
                           1
                           1992.0
     

     






      

          
GC ORFID: 43490How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5549Glimmer Score: 

          
Start: 5781891Stop: 5780056

          
Length: 1836


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA5549 translation of PA5549Homolog Bit Score 1191.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene4136 (ORF: BLASTX 1046.0), t_PA5549 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTGTGGAATCGTAGGTGCGATCGCCGAGCGCAACATCACCCCCATCCTCATCGAAGGCCTCAAGCGCCTC 
GAGTATCGCGGCTACGACAGCGCCGGCGTGGCGGTCTTCGATAACGAAGGCAGGCTGCAGCGCTGCCGCCGG 
GTCGGCAAGGTCGCCAGCCTGGAGGAAGGCCTGGCCGGTACGCCGCTGCTCGGCCGCCTGGGCATCGCCCAT 
ACCCGCTGGGCGACCCACGGCGCGCCAACCGAAGGCAACGCCCACCCGCATTTCTCCAGCGACGAACTGGCG 
GTGGTGCACAACGGCATCATCGAGAACCACGAACCTCTCCGTGAGCGGCTCAAGGGCCTGGGCTACGTGTTC 
ACCTCGCAGACCGACACCGAGGTCATCGTCCACCTGCTGCATCACAAGCTGCAGTCCATCGGCGACCTGACC 
CTGGCGCTGAAGGACGCGGTCAAGGAATTGCACGGGGCCTATGGCCTGGCAGTGATCAGCGCGGCGCAGCCG 
GACCGCATCGTCGCCGCCCGCAGCGGCAGCCCGCTGGTGATCGGCCTCGGGCTTGGCGAGAACTTCCTCGCT 
TCCGACCAACTGGCGCTGCGCCAGGTGACCGACCGCTTCATCTACCTGGAAGAAGGCGATATCGCCGAGATC 
CGTCGCGACAGCGTGCGCCTGTGGGATGTCCAGGGCAACGACGTGCAGCGCGAGACCGTGCAGTACCACGAA 
GGCGCCGAAGCCGCCGACAAGGGCGAGTACCGGCACTTCATGCTCAAGGAAATCCACGAGCAGCCCAGCGTC 
GTGCAGCGCACCCTGGAAGGCCGCCTCGGGCAGAACCAGGTAATGGTGGAAAGCTTCGGCCCGCAGGCGGCG 
GAGCTGTTCGCCAAGGTTCGCAACGTGCAGATCGTCGCCTGCGGTACCAGCTACCACGCCGGCATGGTCGCC 
CGTTACTGGCTGGAAAGCCTGACCGGGATTCCCTGCCAGGTGGAGGTCGCCAGCGAGTTCCGCTACCGCAAG 
GTCGCGGTGCAGCCGGATTGCCTGTTCGTCACCATCTCCCAGTCCGGTGAGACCGCCGACACCCTGGCTGCC 
CTGCGCAACGCCAAGGAGCTGGGCTTCCTCTCCAGCGTGGCGATCTGCAACGTTGCCACCAGTTCGCTGGTG 
CGCGAATCCGACCTGACCCTGCTGACCCAGGCCGGCCCGGAAATCGGCGTGGCCTCGACCAAGGCCTTCACC 
ACCCAGTTGGTCGCCCTGCTGCTGCTGACCCTGGGCATCGGCCAGGTGCAGAAGCGCCTGGCCGATGGCGTC 
GAAGCCGAGCTGGTGGACGAACTGCGTCGCCTGCCGACCCGGCTGGGCGAGGCGCTGGCGATGAACCGGACG 
GTGGAGAAGGTTTCCGAGCTGTTCGCCGAGAAGCACCACACCCTGTTCCTCGGCCGTGGCGCGCAGTTCCCG 
GTGGCCCTGGAAGGCGCGCTGAAGCTCAAGGAAATTTCCTATATCCATGCCGAAGCCTACCCGGCCGGCGAA 
CTCAAGCATGGCCCGCTGGCGCTGGTGGACAGCGACATGCCGGTGGTTACCGTGGCGCCGAACAACGAGCTG 
GTGGAGAAGCTCAAGTCGAACCTGCAGGAAGTCCGCGCCCGCGGCGGCGAACTGGTGGTGTTCGCCGACGAA 
GGCGCGGGCATCGAGGCCGGCGAGGGCACCCATGTGGTGGGCATGCCGCACATCGGCGACGTGCTCTCGCCG 
ATCCTCTACACCATCCCGTTGCAATTGCTCTCGTACCATGTCGCCGTGCTCAAGGGCACCGACGTCGACCAG 
CCGCGCAACCTGGCCAAGTCGGTCACCGTCGAGTAA