ViewGene
Locus Name:
GeneID:
Location:

GeneID:4803
TrivialName: PA14_14740
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_14740
PAO1 Ortholog Locus:PA3813
Sequence Length:387
Protein Length:128
Start: 1255607
Stop: 1255993
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07200.1|AE004799_6 (AE004799) probable iron-binding protein IscU [Pseudomonas aeruginosa PAO1]
Identities = 128/128 (100%), Positives = 128/128 (100%)
Sequence:
ATGGCATATAGCGAAAAGGTCATCGACCACTACGAAAACCCGCGCAACGTCGGCAAGCTCGACGCTGCCGAT
CCCAACGTCGGCACCGGCATGGTCGGCGCGCCGGCCTGCGGCGACGTGATGCGCCTGCAGATCAAGGTCAAC
GAGCAGGGCGTGATCGAAGACGCCAAGTTCAAGACCTACGGTTGCGGTTCCGCGATCGCCTCCAGCTCCCTC
GCCACCGAGTGGATGAAGGGCAAGACCCTGGACGAGGCGGAAACCATCAAGAACACCACCATCGCCGAAGAG
CTGGCCCTGCCGCCGGTGAAGATCCACTGCTCCGTTCTCGCCGAAGACGCCATCAAGGCCGCCGTTCGCGAC
TACAAGCAGAAGAAGGGTTTGCTCTAA
Translation:
MAYSEKVIDHYENPRNVGKLDAADPNVGTGMVGAPACGDVMRLQIKVNEQGVIEDAKFKTYGCGSAIASSSL
ATEWMKGKTLDEAETIKNTTIAEELALPPVKIHCSVLAEDAIKAAVRDYKQKKGLL*
AnnotationID:4808GeneID:4803
AnnotatorUID: diggins
Modification Date/Time: 2005-06-14 21:09:35
Gene Name:
Confidence Code:3
GeneProduct:putative iron-binding protein IscU
Cell Localization:()
Synonyms:iscU
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:KOG3361,pfam01592,COG0822
Secondary Functional Category(ies):energy metabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66468.1|AE016276_3 (AE016777) iron-binding protein IscU [Pseudomonas putida KT2440]
          Length = 128

 Score =  247 bits (630), Expect = 1e-65
 Identities = 121/128 (94%), Positives = 126/128 (98%)

>gb|AAN81505.1|AE016764_187 (AE016764) NifU-like protein [Escherichia coli CFT073]
          Length = 128

 Score =  216 bits (550), Expect = 2e-56
 Identities = 102/125 (81%), Positives = 114/125 (91%)






 
Structural Features:
KOG3361, Iron binding protein involved in Fe-S cluster formation 
[Energy production and conversion]
CD-Length = 157 residues,  78.3% aligned
Score =  225 bits (575), Expect = 2e-60
Identities = 100/123 (81%), Positives = 114/123 (92%)

pfam01592, NifU_N, NifU-like N terminal domain.
CD-Length = 127 residues,  98.4% aligned
Score =  181 bits (461), Expect = 4e-47
Identities = 87/127 (68%), Positives = 100/127 (78%), Gaps = 4/127 (3%)

COG0822, IscU, NifU homolog involved in Fe-S cluster formation 
[Energy production and conversion]
CD-Length = 150 residues,  95.3% aligned
Score =  149 bits (378), Expect = 2e-37
Identities = 80/146 (54%), Positives = 98/146 (67%), Gaps = 22/146 (15%)






   
Genomic Context:






   
Comment:
other possible gene name: nifU


















    ReferenceID:9606




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:26747




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31743




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4803_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:4803
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
7180
                           PA3813_tr
                           translation of PA3813
                           128
                           99.99
                           99.99
                           0
                           657.0
     

     
     
187991
                           gb|AAG07200.1|AE004799_6
                           (AE004799) probable iron-binding protein IscU [Pseudomonas aeruginosa PAO1]
                           128
                           99.99
                           99.99
                           0
                           657.0
     

     
     
187992
                           gb|AAN66468.1|AE016276_3
                           (AE016777) iron-binding protein IscU [Pseudomonas putida KT2440]
                           128
                           94.53
                           98.43
                           0
                           630.0
     

     
     
187993
                           gb|AAO54945.1|
                           (AE016860) iron-binding protein IscU [Pseudomonas syringae pv. tomato str. DC3000]
                           128
                           94.53
                           97.65
                           0
                           627.0
     

     
     
187994
                           gb|AAC24473.1|
                           (AF010139) IscU [Azotobacter vinelandii]
                           128
                           92.96
                           97.65
                           0
                           622.0
     

     






      

          
GC ORFID: 47805How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3813Glimmer Score: 

          
Start: 3698331Stop: 3697945

          
Length: 387


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA3813 translation of PA3813Homolog Bit Score 257.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4518 (ORF: BLASTX 248.0), t_PA3813 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCATATAGCGAAAAGGTCATCGACCACTACGAAAACCCGCGCAACGTCGGCAAGCTCGACGCTGCCGAT 
CCCAACGTCGGCACCGGCATGGTCGGCGCGCCGGCCTGCGGCGACGTGATGCGCCTGCAGATCAAGGTCAAC 
GAGCAGGGCGTGATCGAAGACGCCAAGTTCAAGACCTACGGTTGCGGTTCCGCGATCGCCTCCAGCTCCCTC 
GCCACCGAGTGGATGAAGGGCAAGACCCTGGACGAGGCGGAAACCATCAAGAACACCACCATCGCCGAAGAG 
CTGGCCCTGCCGCCGGTGAAGATCCACTGCTCCGTTCTCGCCGAAGACGCCATCAAGGCCGCCGTTCGCGAC 
TACAAGCAGAAGAAGGGTTTGCTCTAA