ViewGene
Locus Name:
GeneID:
Location:

GeneID:4787
TrivialName: PA14_61050
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_61050
PAO1 Ortholog Locus:PA4614
Sequence Length:414
Protein Length:137
Start: 5444712
Stop: 5445125
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08002.1|AE004875_8 (AE004875) conductance mechanosensitive channel [Pseudomonas aeruginosa PAO1]
Identities = 136/137 (99%), Positives = 137/137 (100%)
Sequence:
ATGGGTCTTCTGAGTGAATTCAAAGCCTTTGCCGTCAAGGGCAATGTCGTGGATATGGCTGTCGGTATCATC
ATCGGCGCGGCCTTCGGCAAGATCGTCTCGTCCTTCGTGGGCGATGTGATCATGCCGCCGATCGGACTGCTG
ATCGGCGGAGTCGACTTCTCCGACCTGGCGATAACATTGAAGGCCGCCGAAGGCGACGTGCCGGCGGTGGTG
CTGGCCTATGGCAAGTTCATTCAGACCATACTCGACTTCGTCATCGTGGCCTTCGCCATCTTCATGGGGGTC
AAGGCGATCAACCGCCTCAAGCGCGAAGAAGCCGTCGCGCCGTCCGAACCGCCAGTGCCGAGTGCCGAGGAA
ACCCTGCTGACCGAGATCCGCGACCTGCTGAAAGCCCAGCAGAACAAGTCATGA
Translation:
MGLLSEFKAFAVKGNVVDMAVGIIIGAAFGKIVSSFVGDVIMPPIGLLIGGVDFSDLAITLKAAEGDVPAVV
LAYGKFIQTILDFVIVAFAIFMGVKAINRLKREEAVAPSEPPVPSAEETLLTEIRDLLKAQQNKS*
AnnotationID:4792GeneID:4787
AnnotatorUID: diggins
Modification Date/Time: 2005-05-06 07:07:45
Gene Name:mscL
Confidence Code:2
GeneProduct:large conductance mechanosensitive channel
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(15) Membrane proteins
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam01741,COG1970
Secondary Functional Category(ies):cell envelope biogenesis
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAC38561.1| (AF029732) large conductance mechanosensitive channel [Pseudomonas
           fluorescens]
          Length = 136

 Score =  249 bits (635), Expect = 2e-66
 Identities = 133/137 (97%), Positives = 135/137 (98%), Gaps = 1/137 (0%)


>gb|AAO58087.1| (AE016872) large conductance mechanosensitive channel protein
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 148

 Score =  221 bits (563), Expect = 5e-58
 Identities = 111/133 (83%), Positives = 124/133 (93%)


>gb|AAN82489.1|AE016767_249 (AE016767) Large-conductance mechanosensitive channel [Escherichia
           coli CFT073]
          Length = 137

 Score =  182 bits (463), Expect = 2e-46
 Identities = 86/137 (62%), Positives = 110/137 (80%)






 
Structural Features:
pfam01741, MscL, Large-conductance mechanosensitive channel, MscL.
CD-Length = 127 residues, 100.0% aligned
Score =  163 bits (415), Expect = 1e-41
Identities = 83/131 (63%), Positives = 106/131 (80%), Gaps = 4/131 (3%)

COG1970, MscL, Large-conductance mechanosensitive channel 
[Cell envelope biogenesis, outer membrane]
CD-Length = 130 residues, 100.0% aligned
Score =  154 bits (392), Expect = 5e-39
Identities = 85/135 (62%), Positives = 106/135 (78%), Gaps = 7/135 (5%)






   
Genomic Context:






   
Comment:


















    ReferenceID:9574




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:26715




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31610




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4787_cdd.html


    		




 
URL:

















    ReferenceID:33358




  
Author/Investigator(s):
        Ma Q, Zhai Y, Schneider JC, Ramseier TM, Saier MH Jr.





  
Title:
        Protein secretion systems of Pseudomonas aeruginosa and P fluorescens.




  
PubMed:
        12659964


MedLine:
        




    
Source:
Biochim Biophys Acta. 2003 Apr 1;1611(1-2):223-33.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12659964

















    ReferenceID:33359




  
Author/Investigator(s):
        Kloda A, Martinac B.





  
Title:
        Mechanosensitive channels of bacteria and archaea share a common ancestral origin.




  
PubMed:
        12046893


MedLine:
        




    
Source:
Eur Biophys J. 2002 Mar;31(1):14-25.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12046893

















    ReferenceID:33360




  
Author/Investigator(s):
        Blount P, Sukharev S, Kung C.





  
Title:
        A mechanosensitive channel protein and its gene in E. coli.




  
PubMed:
        11540118


MedLine:
        




    
Source:
Gravit Space Biol Bull. 1997 Jun;10(2):43-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11540118

















    ReferenceID:33361




  
Author/Investigator(s):
        Sukharev SI, Blount P, Martinac B, Kung C.





  
Title:
        Mechanosensitive channels of Escherichia coli: the MscL gene, protein, and activities.




  
PubMed:
        9074781


MedLine:
        




    
Source:
Annu Rev Physiol. 1997;59:633-57.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9074781

















    ReferenceID:33362




  
Author/Investigator(s):
        Blount P, Sukharev SI, Moe PC, Nagle SK, Kung C.





  
Title:
        Towards an understanding of the structural and functional properties of MscL, a mechanosensitive channel in bacteria.




  
PubMed:
        9004483


MedLine:
        




    
Source:
Biol Cell. 1996;87(1-2):1-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9004483

















    ReferenceID:33363




  
Author/Investigator(s):
        Matsushita O, Jung CM, Okabe A.





  
Title:
        Identification of the gene encoding a mechanosensitive channel MscL homologue in Clostridium perfringens.




  
PubMed:
        7489908


MedLine:
        




    
Source:
Gene. 1995 Nov 7;165(1):147-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7489908

















    ReferenceID:33364




  
Author/Investigator(s):
        Sukharev SI, Blount P, Martinac B, Blattner FR, Kung C.





  
Title:
        A large-conductance mechanosensitive channel in E. coli encoded by mscL alone.




  
PubMed:
        7511799


MedLine:
        




    
Source:
Nature. 1994 Mar 17;368(6468):265-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7511799









           
Homologs By Global Alignment 

           

       



     
Gene ID:4787
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
7163
                           PA4614_tr
                           translation of PA4614
                           137
                           99.27
                           99.99
                           0
                           664.0
     

     
     
187768
                           gb|AAG08002.1|AE004875_8
                           (AE004875) conductance mechanosensitive channel [Pseudomonas aeruginosa PAO1]
                           137
                           99.27
                           99.99
                           0
                           664.0
     

     
     
187769
                           gb|AAC38561.1|
                           (AF029732) large conductance mechanosensitive channel [Pseudomonas fluorescens]
                           137
                           97.08
                           98.54
                           1
                           637.0
     

     
     
187770
                           gb|AAN70218.1|AE016661_4
                           (AE016791) large conductance mechanosensitive channel protein [Pseudomonas putida KT2440]
                           139
                           90.64
                           96.40
                           2
                           620.0
     

     
     
187771
                           gb|AAO58087.1|
                           (AE016872) large conductance mechanosensitive channel protein [Pseudomonas syringae pv. tomato str. DC3000]
                           148
                           75.00
                           84.45
                           11
                           563.0
     

     






      

          
GC ORFID: 47789How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4614Glimmer Score: 

          
Start: 4708392Stop: 4708805

          
Length: 414


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4614 translation of PA4614Homolog Bit Score 260.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5649 (ORF: BLASTX 217.0), t_PA4614 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGGTCTTCTGAGTGAATTCAAAGCCTTTGCCGTCAAGGGCAATGTCGTGGATATGGCTGTCGGTATCATC 
ATCGGCGCGGCCTTCGGCAAGATCGTCTCGTCCTTCGTGGGCGATGTGATCATGCCGCCGATCGGACTGCTG 
ATCGGCGGAGTCGACTTCTCCGACCTGGCGATAACATTGAAGGCCGCCGAAGGCGACGTGCCGGCGGTGGTG 
CTGGCCTATGGCAAGTTCATTCAGACCATACTCGACTTCGTCATCGTGGCCTTCGCCATCTTCATGGGGGTC 
AAGGCGATCAACCGCCTCAAGCGCGAAGAAGCCGTCGCGCCGTCCGAACCGCCAGTGCCGAGTGCCGAGGAA 
ACCCTGCTGACCGAGATCCGCGACCTGCTGAAAGCCCAGCAGAACAAGTCATGA