ViewGene
Locus Name:
GeneID:
Location:

GeneID:4774
TrivialName: PA14_18750
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_18750
PAO1 Ortholog Locus:PA3524
Sequence Length:387
Protein Length:128
Start: 1613952
Stop: 1613566
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06912.1|AE004773_1 (AE004773) lactoylglutathione lyase [Pseudomonas aeruginosa PAO1]
Identities = 127/128 (99%), Positives = 127/128 (99%)
Sequence:
ATGCGCATTCTCCATACCATGATCCGGGTCGGAAACATCGATCGCTCGATCGATTTCTATACCCGGGTGCTA
GGCATGACCCTGCTCAGGAAGAACGACTATCCCGACGGCCAGTTCACCCTGGCCTTCGTCGGCTATGGCAAC
GAGGCGGATTCCGCCGTGATCGAGCTTACCCACAACTGGGGCGTGGATGCCTACGAGATCGGCACCGGCTAC
GGCCATATCGCCATCGAGGTCGACGACGCCTACCAGGCCTGCGACGACATCCGCAACAACGGCGGCCAGGTC
ACCCGCGAAGCCGGGCCGATGAAGCACGGTACCACCGTGATCGCCTTCGTGACCGACCCGGACGGCTACAAG
ATCGAACTGATCCAGAAGTCTTCCTGA
Translation:
MRILHTMIRVGNIDRSIDFYTRVLGMTLLRKNDYPDGQFTLAFVGYGNEADSAVIELTHNWGVDAYEIGTGY
GHIAIEVDDAYQACDDIRNNGGQVTREAGPMKHGTTVIAFVTDPDGYKIELIQKSS*
AnnotationID:4779GeneID:4774
AnnotatorUID: diggins
Modification Date/Time: 2005-07-17 11:11:32
Gene Name:gloA1
Confidence Code:2
GeneProduct:lactoylglutathione lyase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00903,COG0346
Secondary Functional Category(ies):central intermediary metabolism
EC Number:4.4.1.5
Status:ACTIVE
Pathway:
Pyruvate metabolism; 
Glyoxal pathway
Homology:
>emb|CAE40417.1| (BX640411) lactoylglutathione lyase [Bordetella pertussis]
          Length = 131

 Score =  209 bits (531), Expect = 3e-54
 Identities = 94/126 (74%), Positives = 112/126 (88%)

>emb|CAD85338.1| (BX321861) possible gloA; lactoylglutathione lyase [Nitrosomonas
           europaea ATCC 19718]
          Length = 129

 Score =  202 bits (515), Expect = 2e-52
 Identities = 91/128 (71%), Positives = 111/128 (86%)

>gb|AAC74723.1| (AE000260) lactoylglutathione lyase [Escherichia coli K12]
          Length = 135

 Score =  190 bits (483), Expect = 1e-48
 Identities = 88/126 (69%), Positives = 109/126 (86%)
Structural Features:
pfam00903, Glyoxalase, Glyoxalase / Bleomycin resistance protein / 
Dioxygenase superfamily.
CD-Length = 120 residues, 100.0% aligned
Score = 94.8 bits (235), Expect = 3e-21
Identities = 42/125 (33%), Positives = 56/125 (44%), Gaps = 8/125 (6%)

COG0346, GloA, Lactoylglutathione lyase and related lyases 
[Amino acid transport and metabolism]
CD-Length = 138 residues, 100.0% aligned
Score = 67.5 bits (163), Expect = 5e-13
Identities = 34/141 (24%), Positives = 50/141 (35%), Gaps = 19/141 (13%)
Genomic Context:






   
Comment:


















    ReferenceID:9548




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:26689




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32042




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4774_cdd.html


    		




 
URL:

















    ReferenceID:34112




  
Author/Investigator(s):
        Kizil G, Wilks K, Wells D, Ala'Aldeen DA.





  
Title:
        Detection and characterisation of the genes encoding glyoxalase I and II from Neisseria meningitidis.




  
PubMed:
        10882093


MedLine:
        




    
Source:
J Med Microbiol. 2000 Jul;49(7):669-73.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10882093

















    ReferenceID:34113




  
Author/Investigator(s):
        Clugston SL, Daub E, Kinach R, Miedema D, Barnard JF, Honek JF.





  
Title:
        solation and sequencing of a gene coding for glyoxalase I activity from Salmonella typhimurium and comparison with other glyoxalase I sequences.




  
PubMed:
        9047352


MedLine:
        




    
Source:
Gene. 1997 Feb 20;186(1):103-11.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9047352









           
Homologs By Global Alignment 

           

       



     
Gene ID:4774
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
7148
                           PA3524_tr
                           translation of PA3524
                           128
                           99.21
                           99.21
                           0
                           669.0
     

     
     
187509
                           gb|AAG06912.1|AE004773_1
                           (AE004773) lactoylglutathione lyase [Pseudomonas aeruginosa PAO1]
                           128
                           99.21
                           99.21
                           0
                           669.0
     

     
     
187513
                           gb|AAQ59336.1|
                           (AE016915) lactoylglutathione lyase [Chromobacterium violaceum ATCC 12472]
                           129
                           73.64
                           86.82
                           1
                           530.0
     

     
     
187510
                           emb|CAE40417.1|
                           (BX640411) lactoylglutathione lyase [Bordetella pertussis] [Bordetella pertussis Tohama I]
                           131
                           71.75
                           85.49
                           3
                           531.0
     

     
     
187511
                           emb|CAE34457.1|
                           (BX640449) lactoylglutathione lyase [Bordetella bronchiseptica] [Bordetella bronchiseptica RB50]
                           131
                           71.75
                           85.49
                           3
                           531.0
     

     






      

          
GC ORFID: 47776How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3524Glimmer Score: 

          
Start: 3339982Stop: 3340368

          
Length: 387


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3524 translation of PA3524Homolog Bit Score 262.0

          
Other Homologs:  t_PA0710 (ORF: BLASTX 164.0), t_PA3524 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGCATTCTCCATACCATGATCCGGGTCGGAAACATCGATCGCTCGATCGATTTCTATACCCGGGTGCTA 
GGCATGACCCTGCTCAGGAAGAACGACTATCCCGACGGCCAGTTCACCCTGGCCTTCGTCGGCTATGGCAAC 
GAGGCGGATTCCGCCGTGATCGAGCTTACCCACAACTGGGGCGTGGATGCCTACGAGATCGGCACCGGCTAC 
GGCCATATCGCCATCGAGGTCGACGACGCCTACCAGGCCTGCGACGACATCCGCAACAACGGCGGCCAGGTC 
ACCCGCGAAGCCGGGCCGATGAAGCACGGTACCACCGTGATCGCCTTCGTGACCGACCCGGACGGCTACAAG 
ATCGAACTGATCCAGAAGTCTTCCTGA