ViewGene
Locus Name:
GeneID:
Location:

GeneID:4757
TrivialName: PA14_05320
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_05320
PAO1 Ortholog Locus:PA0408
Sequence Length:408
Protein Length:135
Start: 464377
Stop: 464784
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03797.1|AE004478_2 (AE004478) twitching motility protein PilG [Pseudomonas aeruginosa PAO1]
Identities = 135/135 (100%), Positives = 135/135 (100%)
Sequence:
ATGGAACAGCAATCCGACGGTTTGAAAGTGATGGTGATCGACGATTCGAAAACGATTCGTCGCACCGCCGAA
ACACTGCTGAAAAAGGTCGGTTGCGACGTGATCACGGCAATCGACGGCTTCGATGCCCTGGCGAAGATCGCC
GATACCCATCCGAACATCATTTTCGTCGACATCATGATGCCGCGCCTGGATGGCTATCAGACCTGCGCCCTG
ATCAAGAACAACAGCGCGTTCAAGTCCACCCCGGTGATCATGCTGTCCTCCAAGGACGGCCTGTTCGACAAG
GCCAAGGGGCGCATCGTCGGCTCCGACCAGTACCTCACCAAGCCGTTCAGCAAGGAAGAGCTGCTGGGCGCG
ATCAAGGCACACGTACCCAGCTTCACCCCGGTGGACGCCGTTTCCTGA
Translation:
MEQQSDGLKVMVIDDSKTIRRTAETLLKKVGCDVITAIDGFDALAKIADTHPNIIFVDIMMPRLDGYQTCAL
IKNNSAFKSTPVIMLSSKDGLFDKAKGRIVGSDQYLTKPFSKEELLGAIKAHVPSFTPVDAVS*
AnnotationID:4762GeneID:4757
AnnotatorUID: diggins
Modification Date/Time: 2005-03-25 08:08:20
Gene Name:pilG
Confidence Code:1
GeneProduct:type IV pili response regulator PilG
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(28) Two-component regulatory systems
Alternate Gene Product Name:CheY homologue
Functional Category Confidence Code:5
COGs:cd00156
Secondary Functional Category(ies):motility and attachment
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58462.1| (AE016874) type IV pilus response regulator PilG [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 136

 Score =  229 bits (585), Expect = 2e-60
 Identities = 115/132 (87%), Positives = 119/132 (90%)

>gb|AAM37947.1| (AE011954) pilus protein [Xanthomonas axonopodis pv. citri str.
           306]
          Length = 133

 Score =  201 bits (510), Expect = 8e-52
 Identities = 99/119 (83%), Positives = 109/119 (91%)

>gb|AAF84757.1|AE004014_11 (AE004014) pilus protein [Xylella fastidiosa 9a5c]
          Length = 145

 Score =  200 bits (509), Expect = 1e-51
 Identities = 98/119 (82%), Positives = 111/119 (93%)






 
Structural Features:
cd00156, REC, Signal receiver domain
CD-Length = 113 residues, 100.0% aligned
Score =  100 bits (251), Expect = 5e-23
Identities = 45/115 (39%), Positives = 67/115 (58%), Gaps = 2/115 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:9515




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:26655




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33735




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4757_cdd.html


    		




 
URL:

















    ReferenceID:35376




  
Author/Investigator(s):
        Darzins A, Russell MA.





  
Title:
        Molecular genetic analysis of type-4 pilus biogenesis and twitching motility using Pseudomonas aeruginosa as a model system--a review.




  
PubMed:
        9224880


MedLine:
        




    
Source:
Gene. 1997 Jun 11;192(1):109-15.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9224880

















    ReferenceID:35377




  
Author/Investigator(s):
        Mattick JS, Whitchurch CB, Alm RA.





  
Title:
        The molecular genetics of type-4 fimbriae in Pseudomonas aeruginosa--a review.




  
PubMed:
        8955641


MedLine:
        




    
Source:
Gene. 1996 Nov 7;179(1):147-55.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8955641

















    ReferenceID:35378




  
Author/Investigator(s):
        Darzins A.





  
Title:
        The pilG gene product, required for Pseudomonas aeruginosa pilus production and twitching motility, is homologous to the enteric, single-domain response regulator CheY.




  
PubMed:
        8104179


MedLine:
        




    
Source:
J Bacteriol. 1993 Sep;175(18):5934-44.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8104179









           
Homologs By Global Alignment 

           

       



     
Gene ID:4757
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
7128
                           PA0408_tr
                           translation of PA0408
                           135
                           99.99
                           99.99
                           0
                           681.0
     

     
     
187297
                           gb|AAG03797.1|AE004478_2
                           (AE004478) twitching motility protein PilG [Pseudomonas aeruginosa PAO1]
                           135
                           99.99
                           99.99
                           0
                           681.0
     

     
     
187298
                           gb|AAA03067.1|
                           (L10831) CheY homologue [Pseudomonas aeruginosa]
                           135
                           99.99
                           99.99
                           0
                           681.0
     

     
     
187299
                           gb|AAO58462.1|
                           (AE016874) type IV pilus response regulator PilG [Pseudomonas syringae pv. tomato str. DC3000]
                           136
                           85.29
                           88.97
                           1
                           581.0
     

     
     
187304
                           gb|AAN70558.1|AE016698_6
                           (AE016792) type IV pili response regulator PilG [Pseudomonas putida KT2440]
                           141
                           70.92
                           81.56
                           14
                           503.0
     

     






      

          
GC ORFID: 47759How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0408Glimmer Score: 

          
Start: 6265719Stop: 6266126

          
Length: 408


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0408 translation of PA0408Homolog Bit Score 266.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3350 (ORF: BLASTX 229.0), t_PA0408 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGAACAGCAATCCGACGGTTTGAAAGTGATGGTGATCGACGATTCGAAAACGATTCGTCGCACCGCCGAA 
ACACTGCTGAAAAAGGTCGGTTGCGACGTGATCACGGCAATCGACGGCTTCGATGCCCTGGCGAAGATCGCC 
GATACCCATCCGAACATCATTTTCGTCGACATCATGATGCCGCGCCTGGATGGCTATCAGACCTGCGCCCTG 
ATCAAGAACAACAGCGCGTTCAAGTCCACCCCGGTGATCATGCTGTCCTCCAAGGACGGCCTGTTCGACAAG 
GCCAAGGGGCGCATCGTCGGCTCCGACCAGTACCTCACCAAGCCGTTCAGCAAGGAAGAGCTGCTGGGCGCG 
ATCAAGGCACACGTACCCAGCTTCACCCCGGTGGACGCCGTTTCCTGA