ViewGene
Locus Name:
GeneID:
Location:

GeneID:475
TrivialName: PA14_56010
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_56010
PAO1 Ortholog Locus:PA4310
Sequence Length:1890
Protein Length:629
Start: 5000826
Stop: 5002715
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07698.1|AE004847_5 (AE004847) chemotactic transducer PctB [Pseudomonas aeruginosa PAO1]
Identities = 627/629 (99%), Positives = 627/629 (99%)
Sequence:
ATGATCAAAAGTCTCAAGTTCAGTCACAAGATCCTGCTCGCCGCCGCGCTGGTGGTGATCGCGACCTTCTCC
CTCTTCACCCTCTACAACGACTCCCTGCAGCGCACCTCGATCCGCGAGGACCTGGAGGACTACCTGCACGAA
ATGGGCGAGATCACCGCCAGCAACGTGCAGAACTGGCTGAGCGGGCGCATCCTGCTGATCGAGAACCTGGCC
CAGACCCTGGCCCGCGACCACAGCCCGGAGACCACCCAGGCCCTGCTGGAACAGCCGCTGCTGGGCTCGACC
TTCCTCTTCACCTACCTGGGGCAGACCGACGGCACCTACACCGCGCGCCCCACCAGCGACCTGCCGGCCGAC
TACGACCCGCGCCGCCGCCCCTGGTACAACGCCGCCACCAGCGCCGGCCAGACCACCCTCACCGAACCCTAC
ATGGAGCCGGCCATCCACGAGCTGGTACTGACCATCGCCAGCCCGGCGCGCCAGGGCGGCCAGCCGTTCGGC
GTGGTCGGCGGCGACCTCAGCCTGCAGACGGTGGTGAAGATCATCAACTCGCTGGACTTCGGCGGCATGGGC
TACGCCTTCCTGGTCAGCGGCGACGGCAAGATCCTCGTCCATCCGGACAAGGACCAGGTCATGAAGAGCCTC
AGCGACGTCTACCCACGCAACACGCCGAAGATCGGCAGCGGCTTCAGCGAGGCCGAGCTGCACGGCAACACC
CGGATCCTCAGTTTCTCTCCGGTCAAGGGCCTGTCCGGCCTGGACTGGTACATCGGCATCTCGGTGGACAAG
GACAAGGCCTATGCCATGCTCACCAAGCTGCGTACCTCGGCGATCGTCGCCGCGCTGATCGCGGTGGTCGCC
ATCGTCCTCCTCCTCGGCATGCTGATCCGCGTGCTGATGCAACCGCTCACCGACATGGGCCGCGCCATGCAG
GACATCGCCCAGGGCGAAGGCGACCTGACCAAGCGCCTGAAGGTCACCAGCAACGATGAATTCGGGGTCCTG
GCCATTTCCTTCAACCGCTTCGTCGAGCGTATCCACGAATCGATCCGCGAGGTCGCCGGCACCGCCCGTCAA
CTGCACGACGTGGCGCAACTGGTGGTGAACGCCTCGAACTCTTCGATGGCCAACTCCGACGAGCAGTCCAAC
CGCACCAACAGCGTCGCCGCGGCGATCAACGAACTCGGCGCCGCCGCCCAGGAAATCGCCCGCAACGCCGCC
GATGCCTCGCACCACGCATCCGACGCCAACCACCAGGCCGAGGACGGCAAGCAGGTGGTCGAGCAGACCATC
CGCGCGATGAACGAGCTTTCCGAGAAGATCAGCGCCTCCTGCGCCAACATCGAGGCCCTCAACAGCCGCACG
GTGAACATCGGCCAGATCCTCGAAGTGATCAAGGGCATCTCCGAGCAGACCAACCTGCTCGCCCTCAACGCC
GCCATCGAAGCCGCCCGCGCCGGCGAGGCCGGACGCGGCTTCGCGGTGGTCGCCGACGAGGTGCGCAACCTG
GCCCACCGCGCCCAGGAGTCGGCCCAGCAGATCCAGAAGATGATCGAGGAACTGCAGATCGGCGCCCAGGAA
GCGGTCAGCACCATGACCGAAAGCCAGCGCTACAGCCTGGAGAGCGTGGAGATCGCCAACCGTGCCGGCGAA
CGCCTGAGCAGTGTCACCGGACGGATCGCCGAGATCGACGGAATGAACCAGTCGGTGGCCACTGCCACCGAG
GAGCAGACCGCGGTGGTCGATTCGCTGAACATGGACATCACCGAGATCAACACCCTCAACCAGGAAGGCGTG
GAGAACCTCCAGGCCACCCTGCGCGCCTGCGGCGAGCTGGAAACCCAGGCCGGGCGCCTGCGCCAGCTGGTG
GACAGCTTCAAGATCTAA
Translation:
MIKSLKFSHKILLAAALVVIATFSLFTLYNDSLQRTSIREDLEDYLHEMGEITASNVQNWLSGRILLIENLA
QTLARDHSPETTQALLEQPLLGSTFLFTYLGQTDGTYTARPTSDLPADYDPRRRPWYNAATSAGQTTLTEPY
MEPAIHELVLTIASPARQGGQPFGVVGGDLSLQTVVKIINSLDFGGMGYAFLVSGDGKILVHPDKDQVMKSL
SDVYPRNTPKIGSGFSEAELHGNTRILSFSPVKGLSGLDWYIGISVDKDKAYAMLTKLRTSAIVAALIAVVA
IVLLLGMLIRVLMQPLTDMGRAMQDIAQGEGDLTKRLKVTSNDEFGVLAISFNRFVERIHESIREVAGTARQ
LHDVAQLVVNASNSSMANSDEQSNRTNSVAAAINELGAAAQEIARNAADASHHASDANHQAEDGKQVVEQTI
RAMNELSEKISASCANIEALNSRTVNIGQILEVIKGISEQTNLLALNAAIEAARAGEAGRGFAVVADEVRNL
AHRAQESAQQIQKMIEELQIGAQEAVSTMTESQRYSLESVEIANRAGERLSSVTGRIAEIDGMNQSVATATE
EQTAVVDSLNMDITEINTLNQEGVENLQATLRACGELETQAGRLRQLVDSFKI*
AnnotationID:484GeneID:475
AnnotatorUID: diggins
Modification Date/Time: 2005-04-04 08:08:24
Gene Name:pctB
Confidence Code:1
GeneProduct:chemotactic transducer PctB
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(10) Chemotaxis
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:smart00283,pfam00015
Secondary Functional Category(ies):adaptation, protection
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66994.1|AE016327_9 (AE016779) methyl-accepting chemotaxis transducer [Pseudomonas
           putida KT2440]
          Length = 624

 Score =  801 bits (2069), Expect = 0.0
 Identities = 420/629 (66%), Positives = 507/629 (80%), Gaps = 5/629 (0%)

>gb|AAO54592.1| (AE016859) methyl-accepting chemotaxis protein [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 626

 Score =  777 bits (2006), Expect = 0.0
 Identities = 408/626 (65%), Positives = 486/626 (77%), Gaps = 1/626 (0%)








 
Structural Features:
smart00283, MA, Methyl-accepting chemotaxis-like domains 
(chemotaxis sensory transducer). 
CD-Length = 262 residues,  99.6% aligned
Score =  220 bits (562), Expect = 4e-58
Identities = 109/261 (41%), Positives = 153/261 (58%)

pfam00015, MCPsignal, Methyl-accepting chemotaxis protein (MCP) signaling domain.
CD-Length = 251 residues,  99.6% aligned
Score =  180 bits (457), Expect = 5e-46
Identities = 96/250 (38%), Positives = 154/250 (61%)






   
Genomic Context:






   
Comment:


















    ReferenceID:953




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18095




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36673




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:475_cdd.html


    		




 
URL:

















    ReferenceID:36799




  
Author/Investigator(s):
        Taguchi K, Fukutomi H, Kuroda A, Kato J, Ohtake H.





  
Title:
        Genetic identification of chemotactic transducers for amino acids in Pseudomonas aeruginosa.




  
PubMed:
        9353923


MedLine:
        




    
Source:
Microbiology. 1997 Oct;143 ( Pt 10):3223-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9353923









           
Homologs By Global Alignment 

           

       



     
Gene ID:475
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
822
                           PA4310_tr
                           translation of PA4310
                           629
                           99.68
                           99.68
                           0
                           3097.0
     

     
     
34350
                           gb|AAG07698.1|AE004847_5
                           (AE004847) chemotactic transducer PctB [Pseudomonas aeruginosa PAO1]
                           629
                           99.68
                           99.68
                           0
                           3097.0
     

     
     
34351
                           dbj|BAA23413.1|
                           (D86947) chemotactic transducer [Pseudomonas aeruginosa]
                           629
                           99.36
                           99.36
                           0
                           3085.0
     

     
     
34353
                           dbj|BAA09307.1|
                           (D50642) transducer [Pseudomonas aeruginosa]
                           629
                           83.46
                           91.09
                           0
                           2642.0
     

     
     
34352
                           gb|AAG07697.1|AE004847_4
                           (AE004847) chemotactic transducer PctA [Pseudomonas aeruginosa PAO1]
                           629
                           83.46
                           91.09
                           0
                           2642.0
     

     






      

          
GC ORFID: 43477How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4310Glimmer Score: 

          
Start: 4264502Stop: 4266391

          
Length: 1890


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA4310 translation of PA4310Homolog Bit Score 1197.0

          
Other Homologs:  t_PA4309 (ORF: BLASTX 1022.0), t_PA4307 (ORF: BLASTX 899.0), psyr_15may02_Scaffold3_revised_gene4268 (ORF: BLASTX 774.0), psyr_15may02_Scaffold8_revised_gene7599 (ORF: BLASTX 753.0), psyr_15may02_Scaffold1_revised_gene1215 (ORF: BLASTX 738.0), t_PA4310 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGATCAAAAGTCTCAAGTTCAGTCACAAGATCCTGCTCGCCGCCGCGCTGGTGGTGATCGCGACCTTCTCC 
CTCTTCACCCTCTACAACGACTCCCTGCAGCGCACCTCGATCCGCGAGGACCTGGAGGACTACCTGCACGAA 
ATGGGCGAGATCACCGCCAGCAACGTGCAGAACTGGCTGAGCGGGCGCATCCTGCTGATCGAGAACCTGGCC 
CAGACCCTGGCCCGCGACCACAGCCCGGAGACCACCCAGGCCCTGCTGGAACAGCCGCTGCTGGGCTCGACC 
TTCCTCTTCACCTACCTGGGGCAGACCGACGGCACCTACACCGCGCGCCCCACCAGCGACCTGCCGGCCGAC 
TACGACCCGCGCCGCCGCCCCTGGTACAACGCCGCCACCAGCGCCGGCCAGACCACCCTCACCGAACCCTAC 
ATGGAGCCGGCCATCCACGAGCTGGTACTGACCATCGCCAGCCCGGCGCGCCAGGGCGGCCAGCCGTTCGGC 
GTGGTCGGCGGCGACCTCAGCCTGCAGACGGTGGTGAAGATCATCAACTCGCTGGACTTCGGCGGCATGGGC 
TACGCCTTCCTGGTCAGCGGCGACGGCAAGATCCTCGTCCATCCGGACAAGGACCAGGTCATGAAGAGCCTC 
AGCGACGTCTACCCACGCAACACGCCGAAGATCGGCAGCGGCTTCAGCGAGGCCGAGCTGCACGGCAACACC 
CGGATCCTCAGTTTCTCTCCGGTCAAGGGCCTGTCCGGCCTGGACTGGTACATCGGCATCTCGGTGGACAAG 
GACAAGGCCTATGCCATGCTCACCAAGCTGCGTACCTCGGCGATCGTCGCCGCGCTGATCGCGGTGGTCGCC 
ATCGTCCTCCTCCTCGGCATGCTGATCCGCGTGCTGATGCAACCGCTCACCGACATGGGCCGCGCCATGCAG 
GACATCGCCCAGGGCGAAGGCGACCTGACCAAGCGCCTGAAGGTCACCAGCAACGATGAATTCGGGGTCCTG 
GCCATTTCCTTCAACCGCTTCGTCGAGCGTATCCACGAATCGATCCGCGAGGTCGCCGGCACCGCCCGTCAA 
CTGCACGACGTGGCGCAACTGGTGGTGAACGCCTCGAACTCTTCGATGGCCAACTCCGACGAGCAGTCCAAC 
CGCACCAACAGCGTCGCCGCGGCGATCAACGAACTCGGCGCCGCCGCCCAGGAAATCGCCCGCAACGCCGCC 
GATGCCTCGCACCACGCATCCGACGCCAACCACCAGGCCGAGGACGGCAAGCAGGTGGTCGAGCAGACCATC 
CGCGCGATGAACGAGCTTTCCGAGAAGATCAGCGCCTCCTGCGCCAACATCGAGGCCCTCAACAGCCGCACG 
GTGAACATCGGCCAGATCCTCGAAGTGATCAAGGGCATCTCCGAGCAGACCAACCTGCTCGCCCTCAACGCC 
GCCATCGAAGCCGCCCGCGCCGGCGAGGCCGGACGCGGCTTCGCGGTGGTCGCCGACGAGGTGCGCAACCTG 
GCCCACCGCGCCCAGGAGTCGGCCCAGCAGATCCAGAAGATGATCGAGGAACTGCAGATCGGCGCCCAGGAA 
GCGGTCAGCACCATGACCGAAAGCCAGCGCTACAGCCTGGAGAGCGTGGAGATCGCCAACCGTGCCGGCGAA 
CGCCTGAGCAGTGTCACCGGACGGATCGCCGAGATCGACGGAATGAACCAGTCGGTGGCCACTGCCACCGAG 
GAGCAGACCGCGGTGGTCGATTCGCTGAACATGGACATCACCGAGATCAACACCCTCAACCAGGAAGGCGTG 
GAGAACCTCCAGGCCACCCTGCGCGCCTGCGGCGAGCTGGAAACCCAGGCCGGGCGCCTGCGCCAGCTGGTG 
GACAGCTTCAAGATCTAA