ViewGene
Locus Name:
GeneID:
Location:

GeneID:472
TrivialName: PA14_19370
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_19370
PAO1 Ortholog Locus:PA3459
Sequence Length:1770
Protein Length:589
Start: 1675049
Stop: 1673280
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06847.1|AE004766_8 (AE004766) probable glutamine amidotransferase [Pseudomonas aeruginosa PAO1]
Identities = 589/589 (100%), Positives = 589/589 (100%)
Sequence:
ATGTGCGGCATATCAGGAGAGTTTCGTTTCGACAACCAGGCAGCCGACCTCGCAGCGGTCGAACGCATCACC
CACCACCTGGCCCCTCGAGGCCCCGACGCCTGGGGTTTCCATGCCCAGGGCCCGATCGCCCTCGGACATCGA
CGGCTGAAGATCATGGACCTGGCGGAAGCTTCCGGCCAACCGATGATCGATCCCGATCTCGGCCTGGCCATG
GTGTTCAATGGCGCCATCTACAACTACCCCGAACTGCGTGGCGAACTGGAAAGCCTTGGCTATCGGTTCTTC
TCCGGCGGCGACACCGAAGTTCTCTTGAAGGGGTACCATGCCTGGGGCGCCGATTTATTGCCCCGGCTCAAT
GGGATGTTCGCCTTCGCCGTCTGGGAGCGCGATCGCCAGCGGCTGTTCCTGGCCCGTGACCGCCTCGGCATC
AAGCCGCTCTACTACAGCCTCGACCGCTCGCGCCTGCGCTTCGCCTCGAGCCTGCCGGCCCTGCTCAAGGGC
GGCGACATCGCCAGCGACCTCGATCCGCAGGCGCTGAATTTCTACCTCAACTTCCACGCCGTGGTTCCCGCC
CCGCACACCTTGCTGGAGGGCGTGAAGAAGCTGCCGCCGGCGACCTGGATGAGCGTCGACCTCGACGGCAGC
TGCGAGCAGCGCACCTGGTGGACGCTGGACTACGGCCCGCGCCCGGACGAGCGCGAGCTGACGCTCGACGAC
TGGCAGGAGCGCGTCCTCGACGGCCTCCGCGAAGCGGTGGCGATCCGCCAGCGCGCCGCCCGCGAGGTCGGC
GTGCTGCTCTCCGGCGGGGTCGATTCGAGCCTGCTGGTGGGCCTGCTGCACGAAGCCGGGGTGGACAACCTG
CTGACCTTCTCCATCGGCTTCGAGGATGCCGGCGGCGAGCGCGGCGACGAGTTCCAGTATTCCGACCTGATT
GCCGAGCGCTATCACACCCGCCACCATCGCCTGCGCATCGGCGAGCACGAAGTGATCGAGCAGTTGCCGGCG
GCCTTCCGCGCCATGAGCGAGCCGATGGTCAGCCACGACTGCATCGCCTTCTACCTGCTCGCGCGGGAAGTC
TCGAAGCACTGCAAGGTGGTGCAGAGCGGCCAGGGCGCCGACGAGCTGTTCGCCGGCTACCACTGGTATCCG
AAGGTGGATGGCGCCGACGACGCCTTCGCCGCGTACCGCGCGGCGTTCTTCGACCGCGACCACGAGGAATAC
CTGGCCACCGTCGGCGAGCGCTTCCGCGTCGAGGACGTCGCCGGGCGTTTCGTCCGCGACCACTTCGCCAGC
CCCGGCGCCGAGGCCGCGGTGGACAAGGCGCTGCGCCTGGACAGCACGATCATGCTGGTCGACGACCCGGTC
AAGCGGGTCGACAACATGACCATGGCCTGGGGCCTGGAAGCCCGCGTGCCGTTCCTCGACTACCGTCTGGCC
GAGCTGTCGGCGCGCATTCCCGCCCGCTTCAAGCTCGGCGATGGCGGCAAGCAGGTGCTCAAGGGTGCCGCG
CGCAAGGTGATTCCCAGCGAAGTCATCGACCGGCCCAAGGGCTACTTCCCGGTGCCCGGCCTCAAGCACCTG
CAGGGCCGCACCCGCGAATGGGTACGCGAGCTGCTGCTCGACCCGAGCCAGGACCGCGGCCTGTTCCAGCCG
GCGATATTCGATCGCCTGCTGAGCGATCCGGACGGCGACCTCACACCGCTGCGCGGCTCCAAGCTGTGGCAG
CTGGCCGCCCTCAACCTCTGGCTCAGCGAACAGGGACTTTGA
Translation:
MCGISGEFRFDNQAADLAAVERITHHLAPRGPDAWGFHAQGPIALGHRRLKIMDLAEASGQPMIDPDLGLAM
VFNGAIYNYPELRGELESLGYRFFSGGDTEVLLKGYHAWGADLLPRLNGMFAFAVWERDRQRLFLARDRLGI
KPLYYSLDRSRLRFASSLPALLKGGDIASDLDPQALNFYLNFHAVVPAPHTLLEGVKKLPPATWMSVDLDGS
CEQRTWWTLDYGPRPDERELTLDDWQERVLDGLREAVAIRQRAAREVGVLLSGGVDSSLLVGLLHEAGVDNL
LTFSIGFEDAGGERGDEFQYSDLIAERYHTRHHRLRIGEHEVIEQLPAAFRAMSEPMVSHDCIAFYLLAREV
SKHCKVVQSGQGADELFAGYHWYPKVDGADDAFAAYRAAFFDRDHEEYLATVGERFRVEDVAGRFVRDHFAS
PGAEAAVDKALRLDSTIMLVDDPVKRVDNMTMAWGLEARVPFLDYRLAELSARIPARFKLGDGGKQVLKGAA
RKVIPSEVIDRPKGYFPVPGLKHLQGRTREWVRELLLDPSQDRGLFQPAIFDRLLSDPDGDLTPLRGSKLWQ
LAALNLWLSEQGL*
AnnotationID:481GeneID:472
AnnotatorUID: diggins
Modification Date/Time: 2005-04-18 21:09:01
Gene Name:
Confidence Code:3
GeneProduct:putative asparagine synthetase, glutamine-hydrolyzing
Cell Localization:()
Synonyms:asnB
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:cd00712,cd00352,COG0367
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO55153.1| (AE016861) asparagine synthetase, glutamine-hydrolyzing
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 590

 Score =  962 bits (2486), Expect = 0.0
 Identities = 468/590 (79%), Positives = 516/590 (87%), Gaps = 1/590 (0%)

>gb|AAN67370.1|AE016363_2 (AE016780) asparagine synthetase [Pseudomonas putida KT2440]
          Length = 595

 Score =  944 bits (2440), Expect = 0.0
 Identities = 468/595 (78%), Positives = 511/595 (85%), Gaps = 6/595 (1%)

>emb|CAC48863.1| (AL603643) putative asparagine synthetase (glutamine
           amidotransferase) protein [Sinorhizobium meliloti]
          Length = 591

 Score =  650 bits (1677), Expect = 0.0
 Identities = 328/591 (55%), Positives = 419/591 (70%), Gaps = 2/591 (0%)








 
Structural Features:
cd00712, Asn_B, Glutamine amidotransferases class-II (GATase) 
asparagine synthase_B type.
CD-Length = 225 residues,  99.6% aligned
Score =  259 bits (662), Expect = 1e-69
Identities = 115/227 (50%), Positives = 140/227 (61%), Gaps = 4/227 (1%)

cd00352, Gn_AT_II, Glutamine amidotransferases class-II (GATase). 
CD-Length = 219 residues,  94.5% aligned
Score =  133 bits (335), Expect = 6e-32
Identities = 77/209 (36%), Positives = 91/209 (43%), Gaps = 40/209 (19%)

COG0367, AsnB, Asparagine synthase (glutamine-hydrolyzing)
[Amino acid transport and metabolism]
CD-Length = 542 residues, 100.0% aligned
Score =  420 bits (1080), Expect = 3e-118
Identities = 190/594 (31%), Positives = 258/594 (43%), Gaps = 57/594 (9%)






   
Genomic Context:






   
Comment:


















    ReferenceID:947




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18089




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32111




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:472_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:472
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
819
                           PA3459_tr
                           translation of PA3459
                           589
                           99.99
                           99.99
                           0
                           3103.0
     

     
     
34341
                           gb|AAG06847.1|AE004766_8
                           (AE004766) probable glutamine amidotransferase [Pseudomonas aeruginosa PAO1]
                           589
                           99.99
                           99.99
                           0
                           3103.0
     

     
     
34342
                           gb|AAO67512.1|
                           (AY228179) asparagine synthase [Pseudomonas syringae pv. tagetis]
                           590
                           79.49
                           87.28
                           1
                           2489.0
     

     
     
34343
                           gb|AAO55153.1|
                           (AE016861) asparagine synthetase, glutamine-hydrolyzing [Pseudomonas syringae pv. tomato str. DC3000]
                           590
                           79.32
                           87.45
                           1
                           2488.0
     

     
     
34344
                           gb|AAN67370.1|AE016363_2
                           (AE016780) asparagine synthetase [Pseudomonas putida KT2440]
                           595
                           78.65
                           85.88
                           6
                           2447.0
     

     






      

          
GC ORFID: 43474How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3459Glimmer Score: 

          
Start: 3278883Stop: 3280652

          
Length: 1770


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3459 translation of PA3459Homolog Bit Score 1199.0

          
Other Homologs:  psyr_15may02_Scaffold5_revised_gene5988 (ORF: BLASTX 962.0), t_PA3459 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTGCGGCATATCAGGAGAGTTTCGTTTCGACAACCAGGCAGCCGACCTCGCAGCGGTCGAACGCATCACC 
CACCACCTGGCCCCTCGAGGCCCCGACGCCTGGGGTTTCCATGCCCAGGGCCCGATCGCCCTCGGACATCGA 
CGGCTGAAGATCATGGACCTGGCGGAAGCTTCCGGCCAACCGATGATCGATCCCGATCTCGGCCTGGCCATG 
GTGTTCAATGGCGCCATCTACAACTACCCCGAACTGCGTGGCGAACTGGAAAGCCTTGGCTATCGGTTCTTC 
TCCGGCGGCGACACCGAAGTTCTCTTGAAGGGGTACCATGCCTGGGGCGCCGATTTATTGCCCCGGCTCAAT 
GGGATGTTCGCCTTCGCCGTCTGGGAGCGCGATCGCCAGCGGCTGTTCCTGGCCCGTGACCGCCTCGGCATC 
AAGCCGCTCTACTACAGCCTCGACCGCTCGCGCCTGCGCTTCGCCTCGAGCCTGCCGGCCCTGCTCAAGGGC 
GGCGACATCGCCAGCGACCTCGATCCGCAGGCGCTGAATTTCTACCTCAACTTCCACGCCGTGGTTCCCGCC 
CCGCACACCTTGCTGGAGGGCGTGAAGAAGCTGCCGCCGGCGACCTGGATGAGCGTCGACCTCGACGGCAGC 
TGCGAGCAGCGCACCTGGTGGACGCTGGACTACGGCCCGCGCCCGGACGAGCGCGAGCTGACGCTCGACGAC 
TGGCAGGAGCGCGTCCTCGACGGCCTCCGCGAAGCGGTGGCGATCCGCCAGCGCGCCGCCCGCGAGGTCGGC 
GTGCTGCTCTCCGGCGGGGTCGATTCGAGCCTGCTGGTGGGCCTGCTGCACGAAGCCGGGGTGGACAACCTG 
CTGACCTTCTCCATCGGCTTCGAGGATGCCGGCGGCGAGCGCGGCGACGAGTTCCAGTATTCCGACCTGATT 
GCCGAGCGCTATCACACCCGCCACCATCGCCTGCGCATCGGCGAGCACGAAGTGATCGAGCAGTTGCCGGCG 
GCCTTCCGCGCCATGAGCGAGCCGATGGTCAGCCACGACTGCATCGCCTTCTACCTGCTCGCGCGGGAAGTC 
TCGAAGCACTGCAAGGTGGTGCAGAGCGGCCAGGGCGCCGACGAGCTGTTCGCCGGCTACCACTGGTATCCG 
AAGGTGGATGGCGCCGACGACGCCTTCGCCGCGTACCGCGCGGCGTTCTTCGACCGCGACCACGAGGAATAC 
CTGGCCACCGTCGGCGAGCGCTTCCGCGTCGAGGACGTCGCCGGGCGTTTCGTCCGCGACCACTTCGCCAGC 
CCCGGCGCCGAGGCCGCGGTGGACAAGGCGCTGCGCCTGGACAGCACGATCATGCTGGTCGACGACCCGGTC 
AAGCGGGTCGACAACATGACCATGGCCTGGGGCCTGGAAGCCCGCGTGCCGTTCCTCGACTACCGTCTGGCC 
GAGCTGTCGGCGCGCATTCCCGCCCGCTTCAAGCTCGGCGATGGCGGCAAGCAGGTGCTCAAGGGTGCCGCG 
CGCAAGGTGATTCCCAGCGAAGTCATCGACCGGCCCAAGGGCTACTTCCCGGTGCCCGGCCTCAAGCACCTG 
CAGGGCCGCACCCGCGAATGGGTACGCGAGCTGCTGCTCGACCCGAGCCAGGACCGCGGCCTGTTCCAGCCG 
GCGATATTCGATCGCCTGCTGAGCGATCCGGACGGCGACCTCACACCGCTGCGCGGCTCCAAGCTGTGGCAG 
CTGGCCGCCCTCAACCTCTGGCTCAGCGAACAGGGACTTTGA