ViewGene
Locus Name:
GeneID:
Location:

GeneID:4691
TrivialName: PA14_69970
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_69970
PAO1 Ortholog Locus:PA5300
Sequence Length:411
Protein Length:136
Start: 6243014
Stop: 6242604
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08685.1|AE004943_1 (AE004943) cytochrome c5 [Pseudomonas aeruginosa PAO1]
Identities = 136/136 (100%), Positives = 136/136 (100%)
Sequence:
ATGCTGGCGGCCCAAGCTGCCGTGTTGGCCCTGTGGGCTGTAAGCGCTCAGGCTACGACCACTGACGATGCG
ATCGCCAAGCGCCTCGAGCCGGTAGGCAAGGTCTGCGTACAAGGCAAGGAATGCGAAGGCGTCGGCGCCGTC
GCGGCAGCCGCCGGTGGCGGCGGTGCGCGTTCCGGCGAAGACATCGTCGGCAAGACCTGCAACACCTGCCAC
GGCACTGGCCTGCTGGGCGCGCCGAAGGTTGGCGACAAGGCCGAGTGGGGCAAGCGCGCCAAGGAGCAGGGC
GGCCTCGATGGCCTGCTGGCCAAGGCGATTTCCGGTATCAACGCCATGCCGCCGAAAGGCACCTGCGCCGAT
TGCTCGGACGACGAGCTGAAAGCCGCGATCAAGCATATGTCCGGCCTGTAA
Translation:
MLAAQAAVLALWAVSAQATTTDDAIAKRLEPVGKVCVQGKECEGVGAVAAAAGGGGARSGEDIVGKTCNTCH
GTGLLGAPKVGDKAEWGKRAKEQGGLDGLLAKAISGINAMPPKGTCADCSDDELKAAIKHMSGL*
AnnotationID:4697GeneID:4691
AnnotatorUID: diggins
Modification Date/Time: 2005-03-16 12:12:14
Gene Name:cycB
Confidence Code:2
GeneProduct:cytochrome c5
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG3245
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAC45922.1| (U94420) cytochrome c5 [Azotobacter vinelandii]
          Length = 134

 Score =  194 bits (493), Expect = 7e-50
 Identities = 98/136 (72%), Positives = 109/136 (80%), Gaps = 2/136 (1%)

>gb|AAN70832.1|AE016728_1 (AE016793) cytochrome c5 [Pseudomonas putida KT2440]
          Length = 121

 Score =  184 bits (467), Expect = 7e-47
 Identities = 89/123 (72%), Positives = 106/123 (86%), Gaps = 2/123 (1%)

>gb|AAO53660.1| (AE016856) cytochrome c5 [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 107

 Score =  110 bits (274), Expect = 2e-24
 Identities = 50/86 (58%), Positives = 61/86 (70%)








 
Structural Features:
COG3245, CycB, Cytochrome c5 [Energy production and conversion]
CD-Length = 126 residues,  96.0% aligned
Score =  106 bits (266), Expect = 8e-25
Identities = 60/131 (45%), Positives = 68/131 (51%), Gaps = 10/131 (7%)






   
Genomic Context:






   
Comment:


















    ReferenceID:9383




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:26523




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34798




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4691_cdd.html


    		




 
URL:

















    ReferenceID:35711




  
Author/Investigator(s):
        Rossbach S, Loferer H, Acuna G, Appleby CA, Hennecke H.





  
Title:
        Cloning, sequencing and mutational analysis of the cytochrome c552 gene (cycB) from Bradyrhizobium japonicum strain 110.




  
PubMed:
        1663888


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1991 Oct 1;67(2):145-52.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1663888

















    ReferenceID:35712




  
Author/Investigator(s):
        Ras J, Reijnders WN, Van Spanning RJ, Harms N, Oltmann LF, Stouthamer AH.





  
Title:
        Isolation, sequencing, and mutagenesis of the gene encoding cytochrome c553i of Paracoccus denitrificans and characterization of the mutant strain.




  
PubMed:
        1657873


MedLine:
        




    
Source:
J Bacteriol. 1991 Nov;173(21):6971-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1657873

















    ReferenceID:35713




  
Author/Investigator(s):
        Keightley JA, Sanders D, Todaro TR, Pastuszyn A, Fee JA.





  
Title:
        Cloning and expression in Escherichia coli of the cytochrome c552 gene from Thermus thermophilus HB8. Evidence for genetic linkage to an ATP-binding cassette protein and initial characterization of the cycA gene products.




  
PubMed:
        9575141


MedLine:
        




    
Source:
J Biol Chem. 1998 May 15;273(20):12006-16.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9575141









           
Homologs By Global Alignment 

           

       



     
Gene ID:4691
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
7060
                           PA5300_tr
                           translation of PA5300
                           136
                           99.99
                           99.99
                           0
                           706.0
     

     
     
186507
                           gb|AAG08685.1|AE004943_1
                           (AE004943) cytochrome c5 [Pseudomonas aeruginosa PAO1]
                           136
                           99.99
                           99.99
                           0
                           706.0
     

     
     
186508
                           gb|AAC45922.1|
                           (U94420) cytochrome c5 [Azotobacter vinelandii]
                           136
                           72.05
                           80.14
                           2
                           497.0
     

     
     
186509
                           gb|AAN70832.1|AE016728_1
                           (AE016793) cytochrome c5 [Pseudomonas putida KT2440]
                           136
                           65.44
                           77.94
                           15
                           471.0
     

     
     
186511
                           gb|AAF93344.1|
                           (AE004107) cytochrome c5 [Vibrio cholerae O1 biovar eltor str. N16961]
                           144
                           41.66
                           54.16
                           17
                           271.0
     

     






      

          
GC ORFID: 47693How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5300Glimmer Score: 

          
Start: 5506703Stop: 5506293

          
Length: 411


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA5300 translation of PA5300Homolog Bit Score 276.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3161 (ORF: BLASTX 111.0), t_PA5300 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCTGGCGGCCCAAGCTGCCGTGTTGGCCCTGTGGGCTGTAAGCGCTCAGGCTACGACCACTGACGATGCG 
ATCGCCAAGCGCCTCGAGCCGGTAGGCAAGGTCTGCGTACAAGGCAAGGAATGCGAAGGCGTCGGCGCCGTC 
GCGGCAGCCGCCGGTGGCGGCGGTGCGCGTTCCGGCGAAGACATCGTCGGCAAGACCTGCAACACCTGCCAC 
GGCACTGGCCTGCTGGGCGCGCCGAAGGTTGGCGACAAGGCCGAGTGGGGCAAGCGCGCCAAGGAGCAGGGC 
GGCCTCGATGGCCTGCTGGCCAAGGCGATTTCCGGTATCAACGCCATGCCGCCGAAAGGCACCTGCGCCGAT 
TGCTCGGACGACGAGCTGAAAGCCGCGATCAAGCATATGTCCGGCCTGTAA