ViewGene
Locus Name:
GeneID:
Location:

GeneID:467
TrivialName: PA14_36580
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_36580
PAO1 Ortholog Locus:PA2164
Sequence Length:1752
Protein Length:583
Start: 3255048
Stop: 3256799
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05552.1|AE004643_5 (AE004643) probable glycosyl hydrolase [Pseudomonas aeruginosa PAO1]
Identities = 577/583 (98%), Positives = 578/583 (99%)
Sequence:
ATGGCGATCCGGCGCCGCTTCGGCGCCCAGTTCCAAGGCAACGGCCGAACCTGCTTCGGCCTCTGGGCTCCG
GACGCCCGCGAGGTCCGGGTCGAGACCGCCGACGGCCGCGACTGGCCGCTGGAAGGCAGCGACAGCGGCTGG
TTCGAGGCGACCCTGCCCTGTCCGCCCGGCACCCGCTACCGCTACCGCATCGATGGCCGCCCCGGGGTGCCG
GACCCGGCCTCGCAGTTCCAGCCCGACGGCGTGCATGGCCACAGCCAGGTGCTGGACCATGACGCCTACGCC
TGGCGCGTCGACGAGTGGCGCGGGCGGCCTTGGCACGAAGCGGTGATCTACGAGCTGCACGTCGGCCTGTTC
GGCTCCTACGCCGAGGTCGAGGGCTTCCTTCCGCGCCTGGTGGAACTGGGCGTGACCGCCGTGGAGCTGATG
CCGCTGGGCGAGTTTCCCGGTCGCCGCAACTGGGGCTACGACGGCGTGCTGCCGTTCGCCCCGGCGTCGGCC
TACGGCACGCCCGAGCAACTCAAGCATCTGATCGACAGCGCTCACGGCATGGGCCTGATGGTCTTCGTCGAC
GTGATCTACAACCACTTCGGCCCGGACGGCAACTACCTCGCCCAGTACGCCGCCGCGTTCTTCCGCGACGAC
CGGCAGACGCCCTGGGGCCAGGCCATCGATTTCCGTCGCGGCGAAGTCCGCGAGTTCTTCTATGAGAACGCC
CTGATGTGGCTGCTCGACTATCGCGTCGACGGCCTGCGCTTCGATGCCGTGCACGCGATTCCCGATAGCGCG
TTCCTCGTCGAGATGGCCCGCCGCCTGCGCGGCGCCGCCGGCCCCGAGCGCCATGTGCACCTGGTCCTGGAG
AACGACGACAACCGCGCCAGCCTGCTGCGCCAGGGCTACGACGCACAGTGGAACGACGATGGCCACCATGCG
CTGCACGTCCTGCTCACCGGCGAGAGCGACGGCTACTACCAGGACTACCCCGAGCCGCTGCGGTGCCTGGCG
CGCTGCCTGGCCGAAGGCTTCGTCTACCAGGGCGAAGCCAACCGCCACGGCCGGCCGCGCGGCGAACCGAGT
GCCGACCTGGCGCCGGACGCCTTCGTGCTGTTCCTGCAAAACCACGACCAGGTCGGCAACCGCGCCTTCGGC
GAGCGTCTCAGCGTTCTCGCCGAACCCCAGGCGTTGCGCCTGGCGATAGCGCTGCAACTGCTCGCGCCGATG
ATCCCGCTGCTGTTCATGGGCGAGGAATGCGCCGCGCGCGAACCGTTCCTGTATTTCACCGATCACCAGGGC
GAACTGGCGGACGCGGTCCGCGAGGGACGACGCAAGGAGTTCGGCGAGTTCGGCCGCTTCGGCGAGGGCGCC
ACCCTGGCTTCGCTCCCCGATCCCAACGCGGTGGAGGCCTTCGAGCGCTCGCGTCCCGGCCTGGCCGATTGC
GACCCGGCCTGGCGCGGCTTCTACCGGCAACTGCTGGAAATCCGTCACGAACACCTGATCCCGCGCCTGCGC
GGCGCCCGTTCGCTGGGCGTGACCACCATCGCCGGCGCCGCGCTGTCGGCGCGCTGGCGACTCGGTGACGGC
AGTGACTGGCGAATCGACTTCAACCCTTCTCCGCTCGAAGCGGAAGTCGAGCCGCCGGCGGCCGCCACCCAG
GTGCTGTTCCTCGACAACCTCGCCTGTGCCGAGTATCGCCGCGGCCGCCTAAGTCCCCGCAGCATCGCGGTC
AGCCTGGAGCCCGCCCATGAGTGA
Translation:
MAIRRRFGAQFQGNGRTCFGLWAPDAREVRVETADGRDWPLEGSDSGWFEATLPCPPGTRYRYRIDGRPGVP
DPASQFQPDGVHGHSQVLDHDAYAWRVDEWRGRPWHEAVIYELHVGLFGSYAEVEGFLPRLVELGVTAVELM
PLGEFPGRRNWGYDGVLPFAPASAYGTPEQLKHLIDSAHGMGLMVFVDVIYNHFGPDGNYLAQYAAAFFRDD
RQTPWGQAIDFRRGEVREFFYENALMWLLDYRVDGLRFDAVHAIPDSAFLVEMARRLRGAAGPERHVHLVLE
NDDNRASLLRQGYDAQWNDDGHHALHVLLTGESDGYYQDYPEPLRCLARCLAEGFVYQGEANRHGRPRGEPS
ADLAPDAFVLFLQNHDQVGNRAFGERLSVLAEPQALRLAIALQLLAPMIPLLFMGEECAAREPFLYFTDHQG
ELADAVREGRRKEFGEFGRFGEGATLASLPDPNAVEAFERSRPGLADCDPAWRGFYRQLLEIRHEHLIPRLR
GARSLGVTTIAGAALSARWRLGDGSDWRIDFNPSPLEAEVEPPAAATQVLFLDNLACAEYRRGRLSPRSIAV
SLEPAHE*
AnnotationID:476GeneID:467
AnnotatorUID: danlee
Modification Date/Time: 2006-03-13 12:12:13
Gene Name:
Confidence Code:3
GeneProduct:putative glycosyl hydrolase
Cell Localization:()
Synonyms:PA2164, glgB
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(20) Putative enzymes
Alternate Gene Product Name:putative 1,4-alpha-glucan branching enzyme
Functional Category Confidence Code:5
COGs:COG0296
Secondary Functional Category(ies):carbohydrate transport and metabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69641.1|AE016598_2 (AE016789) alpha-amylase family protein [Pseudomonas putida KT2440]
          Length = 580

 Score =  686 bits (1771), Expect = 0.0
 Identities = 336/549 (61%), Positives = 394/549 (71%), Gaps = 2/549 (0%)

>gb|AAM35318.1| (AE011669) maltooligosyltrehalose trehalohydrolase [Xanthomonas
           axonopodis pv. citri str. 306]
          Length = 585

 Score =  593 bits (1530), Expect = e-169
 Identities = 303/571 (53%), Positives = 379/571 (66%), Gaps = 16/571 (2%)

>emb|CAE33358.1| (BX640445) probable alpha amylase [Bordetella bronchiseptica]
          Length = 584

 Score =  538 bits (1385), Expect = e-152
 Identities = 296/552 (53%), Positives = 351/552 (63%), Gaps = 18/552 (3%)






 
Structural Features:
COG0296, GlgB, 1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism]
CD-Length = 628 residues,  97.0% aligned
Score =  502 bits (1294), Expect = 5e-143
Identities = 203/626 (32%), Positives = 273/626 (43%), Gaps = 63/626 (10%)







   
Genomic Context:






   
Comment:


















    ReferenceID:937




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18079




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32565




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:467_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:467
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
803
                           PA2164_tr
                           translation of PA2164
                           583
                           98.97
                           99.14
                           0
                           3114.0
     

     
     
34242
                           gb|AAG05552.1|AE004643_5
                           (AE004643) probable glycosyl hydrolase [Pseudomonas aeruginosa PAO1]
                           583
                           98.97
                           99.14
                           0
                           3114.0
     

     
     
34243
                           gb|AAN69641.1|AE016598_2
                           (AE016789) alpha-amylase family protein [Pseudomonas putida KT2440]
                           587
                           58.77
                           69.16
                           11
                           1778.0
     

     
     
34244
                           gb|AAO56613.1|
                           (AE016867) alpha-amylase family protein [Pseudomonas syringae pv. tomato str. DC3000]
                           613
                           55.62
                           65.41
                           39
                           1729.0
     

     
     
34246
                           emb|CAD17388.1|
                           (AL646077) PUTATIVE MALTOOLIGOSYL TREHALOSE TREHALOHYDROLASE PROTEIN [Ralstonia solanacearum]
                           625
                           51.52
                           59.84
                           45
                           1541.0
     

     






      

          
GC ORFID: 43469How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2164Glimmer Score: 

          
Start: 1698867Stop: 1697116

          
Length: 1752


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2164 translation of PA2164Homolog Bit Score 1204.0

          
Other Homologs:  psyr_15may02_Scaffold7_revised_gene7219 (ORF: BLASTX 677.0), t_PA2164 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCGATCCGGCGCCGCTTCGGCGCCCAGTTCCAAGGCAACGGCCGAACCTGCTTCGGCCTCTGGGCTCCG 
GACGCCCGCGAGGTCCGGGTCGAGACCGCCGACGGCCGCGACTGGCCGCTGGAAGGCAGCGACAGCGGCTGG 
TTCGAGGCGACCCTGCCCTGTCCGCCCGGCACCCGCTACCGCTACCGCATCGATGGCCGCCCCGGGGTGCCG 
GACCCGGCCTCGCAGTTCCAGCCCGACGGCGTGCATGGCCACAGCCAGGTGCTGGACCATGACGCCTACGCC 
TGGCGCGTCGACGAGTGGCGCGGGCGGCCTTGGCACGAAGCGGTGATCTACGAGCTGCACGTCGGCCTGTTC 
GGCTCCTACGCCGAGGTCGAGGGCTTCCTTCCGCGCCTGGTGGAACTGGGCGTGACCGCCGTGGAGCTGATG 
CCGCTGGGCGAGTTTCCCGGTCGCCGCAACTGGGGCTACGACGGCGTGCTGCCGTTCGCCCCGGCGTCGGCC 
TACGGCACGCCCGAGCAACTCAAGCATCTGATCGACAGCGCTCACGGCATGGGCCTGATGGTCTTCGTCGAC 
GTGATCTACAACCACTTCGGCCCGGACGGCAACTACCTCGCCCAGTACGCCGCCGCGTTCTTCCGCGACGAC 
CGGCAGACGCCCTGGGGCCAGGCCATCGATTTCCGTCGCGGCGAAGTCCGCGAGTTCTTCTATGAGAACGCC 
CTGATGTGGCTGCTCGACTATCGCGTCGACGGCCTGCGCTTCGATGCCGTGCACGCGATTCCCGATAGCGCG 
TTCCTCGTCGAGATGGCCCGCCGCCTGCGCGGCGCCGCCGGCCCCGAGCGCCATGTGCACCTGGTCCTGGAG 
AACGACGACAACCGCGCCAGCCTGCTGCGCCAGGGCTACGACGCACAGTGGAACGACGATGGCCACCATGCG 
CTGCACGTCCTGCTCACCGGCGAGAGCGACGGCTACTACCAGGACTACCCCGAGCCGCTGCGGTGCCTGGCG 
CGCTGCCTGGCCGAAGGCTTCGTCTACCAGGGCGAAGCCAACCGCCACGGCCGGCCGCGCGGCGAACCGAGT 
GCCGACCTGGCGCCGGACGCCTTCGTGCTGTTCCTGCAAAACCACGACCAGGTCGGCAACCGCGCCTTCGGC 
GAGCGTCTCAGCGTTCTCGCCGAACCCCAGGCGTTGCGCCTGGCGATAGCGCTGCAACTGCTCGCGCCGATG 
ATCCCGCTGCTGTTCATGGGCGAGGAATGCGCCGCGCGCGAACCGTTCCTGTATTTCACCGATCACCAGGGC 
GAACTGGCGGACGCGGTCCGCGAGGGACGACGCAAGGAGTTCGGCGAGTTCGGCCGCTTCGGCGAGGGCGCC 
ACCCTGGCTTCGCTCCCCGATCCCAACGCGGTGGAGGCCTTCGAGCGCTCGCGTCCCGGCCTGGCCGATTGC 
GACCCGGCCTGGCGCGGCTTCTACCGGCAACTGCTGGAAATCCGTCACGAACACCTGATCCCGCGCCTGCGC 
GGCGCCCGTTCGCTGGGCGTGACCACCATCGCCGGCGCCGCGCTGTCGGCGCGCTGGCGACTCGGTGACGGC 
AGTGACTGGCGAATCGACTTCAACCCTTCTCCGCTCGAAGCGGAAGTCGAGCCGCCGGCGGCCGCCACCCAG 
GTGCTGTTCCTCGACAACCTCGCCTGTGCCGAGTATCGCCGCGGCCGCCTAAGTCCCCGCAGCATCGCGGTC 
AGCCTGGAGCCCGCCCATGAGTGA