ViewGene
Locus Name:
GeneID:
Location:

GeneID:4615
TrivialName: PA14_66940
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_66940
PAO1 Ortholog Locus:PA5066
Sequence Length:405
Protein Length:134
Start: 5977178
Stop: 5977582
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08451.1|AE004920_1 (AE004920) phosphoribosyl-AMP cyclohydrolase [Pseudomonas aeruginosa PAO1]
Identities = 133/134 (99%), Positives = 134/134 (100%)
Sequence:
ATGAAAGACTGGCTGGATGAGATTCACTGGAACGCCGACGGCCTGGTCCCGGCGATCGCCCAGGATCACGAG
ACCGGGCGCGTGCTGATGATGGCCTGGATGAACCGCGAGGCGCTGGCCCTGACCGCCAGCGAGAACCGTGCA
ATCTATTGGTCGCGTTCGCGTGGCAAGCTGTGGCGCAAGGGCGAGGAGTCCGGGCATGTGCAGAAGCTGCAC
GAACTGCGCCTGGACTGCGACGCCGATGTGGTCATCCTGATGGTCGAGCAGGTCGGCGGGATCGCCTGCCAT
ACCGGCCGGGAAAGCTGTTTCTACCGCGTCTTCGAGAACGGCGCCTGGAAGACCATCGATCCGGTGCTGAAG
GACCCGGACGCCATCTACGAACACGCAGGACACCACCATGAGTGA
Translation:
MKDWLDEIHWNADGLVPAIAQDHETGRVLMMAWMNREALALTASENRAIYWSRSRGKLWRKGEESGHVQKLH
ELRLDCDADVVILMVEQVGGIACHTGRESCFYRVFENGAWKTIDPVLKDPDAIYEHAGHHHE*
AnnotationID:4621GeneID:4615
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 12:12:37
Gene Name:hisI
Confidence Code:2
GeneProduct:phosphoribosyl-AMP cyclohydrolase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam01502
Secondary Functional Category(ies):amino acid transport and metabolism
EC Number:3.5.4.19
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58579.1| (AE016874) phosphoribosyl-AMP cyclohydrolase [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 130

 Score =  255 bits (651), Expect = 3e-68
 Identities = 118/131 (90%), Positives = 125/131 (95%), Gaps = 1/131 (0%)

>gb|AAN70579.1|AE016700_7 (AE016792) phosphoribosyl-AMP cyclohydrolase [Pseudomonas putida
           KT2440]
          Length = 130

 Score =  248 bits (632), Expect = 5e-66
 Identities = 115/131 (87%), Positives = 124/131 (94%), Gaps = 1/131 (0%)

>emb|CAE35223.1| (BX640451) phosphoribosyl-AMP cyclohydrolase [Bordetella
           bronchiseptica]
          Length = 134

 Score =  184 bits (467), Expect = 7e-47
 Identities = 86/128 (67%), Positives = 103/128 (80%), Gaps = 4/128 (3%)








 
Structural Features:
pfam01502, PRA-CH, Phosphoribosyl-AMP cyclohydrolase. This enzyme catalyses the 
third step in the histidine biosynthetic pathway.
CD-Length = 75 residues, 100.0% aligned
Score =  121 bits (306), Expect = 2e-29
Identities = 46/76 (60%), Positives = 56/76 (73%), Gaps = 1/76 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:9231




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:26372




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34999




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4615_cdd.html


    		




 
URL:

















    ReferenceID:35825




  
Author/Investigator(s):
        Oriol E, Mendez-Alvarez S, Barbe J, Gibert I.





  
Title:
        Cloning of the Rhodobacter sphaeroides hisL gene: unifunctionality of the encoded protein and lack of linkage to other his genes.




  
PubMed:
        8760919


MedLine:
        




    
Source:
Microbiology. 1996 Aug;142 ( Pt 8):2071-8.

Erratum in:
    * Microbiology 1997 May;143(Pt 5):1779.








    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8760919









           
Homologs By Global Alignment 

           

       



     
Gene ID:4615
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6983
                           PA5066_tr
                           translation of PA5066
                           134
                           99.25
                           99.99
                           0
                           737.0
     

     
     
185405
                           gb|AAG08451.1|AE004920_1
                           (AE004920) phosphoribosyl-AMP cyclohydrolase [Pseudomonas aeruginosa PAO1]
                           134
                           99.25
                           99.99
                           0
                           737.0
     

     
     
185407
                           gb|AAO58579.1|
                           (AE016874) phosphoribosyl-AMP cyclohydrolase [Pseudomonas syringae pv. tomato str. DC3000]
                           134
                           88.05
                           93.28
                           4
                           653.0
     

     
     
185406
                           gb|AAA92106.1|
                           (U48404) ORF2; similar to the N-terminal of phosphoribosyl c-AMP hydrolase [Azotobacter chroococcum]
                           134
                           85.82
                           95.52
                           1
                           663.0
     

     
     
185408
                           gb|AAN70579.1|AE016700_7
                           (AE016792) phosphoribosyl-AMP cyclohydrolase [Pseudomonas putida KT2440]
                           134
                           85.82
                           92.53
                           4
                           634.0
     

     






      

          
GC ORFID: 47617How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5066Glimmer Score: 

          
Start: 5240862Stop: 5241266

          
Length: 405


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5066 translation of PA5066Homolog Bit Score 288.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3797 (ORF: BLASTX 257.0), t_PA5066 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAAGACTGGCTGGATGAGATTCACTGGAACGCCGACGGCCTGGTCCCGGCGATCGCCCAGGATCACGAG 
ACCGGGCGCGTGCTGATGATGGCCTGGATGAACCGCGAGGCGCTGGCCCTGACCGCCAGCGAGAACCGTGCA 
ATCTATTGGTCGCGTTCGCGTGGCAAGCTGTGGCGCAAGGGCGAGGAGTCCGGGCATGTGCAGAAGCTGCAC 
GAACTGCGCCTGGACTGCGACGCCGATGTGGTCATCCTGATGGTCGAGCAGGTCGGCGGGATCGCCTGCCAT 
ACCGGCCGGGAAAGCTGTTTCTACCGCGTCTTCGAGAACGGCGCCTGGAAGACCATCGATCCGGTGCTGAAG 
GACCCGGACGCCATCTACGAACACGCAGGACACCACCATGAGTGA