ViewGene
Locus Name:
GeneID:
Location:

GeneID:459
TrivialName: PA14_04630
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_04630
PAO1 Ortholog Locus:PA0353
Sequence Length:1839
Protein Length:612
Start: 412056
Stop: 410218
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03742.1|AE004473_4 (AE004473) dihydroxy-acid dehydratase [Pseudomonas aeruginosa PAO1]
Identities = 612/612 (100%), Positives = 612/612 (100%)
Sequence:
ATGCCCGATTACCGCTCGAAAACCTCCACCCACGGCCGCAACATGGCCGGCGCCCGCGCCCTCTGGCGCGCC
ACCGGGATGAAGGACGAAGACTTCAAGAAGCCGATCATCGCCATCGCCAACTCCTTCACCCAGTTCGTGCCC
GGCCACGTGCACCTGAAGGACCTCGGCCAACTGGTCGCCCGCGAGATCGAGAAGGCCGGCGGCGTGGCCAAG
GAATTCAACACCATCGCCGTCGACGACGGCATCGCCATGGGCCACGACGGCATGCTCTATTCGCTGCCGAGC
CGGGAGATCATCGCCGACTCCGTGGAATACATGGTCAACGCCCACTGCGCCGACGCCATCGTCTGCATCTCC
AACTGCGACAAGATCACCCCCGGCATGCTGATGGCGGCGCTGCGCCTGAACATCCCGGTAGTGTTCGTCTCC
GGCGGGCCGATGGAAGCCGGCAAGACCAAGCTGGCCAGCCACGGCCTGGACCTGGTCGATGCCATGGTGGTG
GCCGCCGACGACTCCTGCTCCGACGAGAAGGTCGCCGAGTACGAGCGCAGCGCCTGCCCGACCTGCGGCTCC
TGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAGGCGCTCGGCCTGTCGCTGCCGGGCAAC
GGCTCGACCCTGGCCACCCACGCCGACCGCGAGCAACTGTTCCTGCGCGCCGGGCGCCTGGCCGTGGAACTC
TGCCAGCGCTACTACGGCGAAGGCGACGACAGCGTGCTGCCGCGCAACATCGCCAACTTCAAGGCCTTCGAG
AACGCCATGACCCTGGACATCGCCATGGGCGGCTCGACCAATACCATCCTGCACCTGCTGGCCGCCGCCCAG
GAGGCCGAGGTGCCGTTCGACCTGCGCGACATCGACCGCCTGTCACGCAAGGTGCCACAACTGTGCAAGGTG
GCGCCGAACATCCAGAAGTACCACATGGAAGACGTGCATCGCGCCGGCGGCATCTTCTCCATCCTCGGCGAA
CTGGCCCGCGGTGGCCTGCTGCACACCGACGTCCCCACCGTGCACAGCCCGAGCATGGCCGACGCCATCGCC
CAGTGGGACATCACCCAGACCCGCGACGAAGCCGTGCACACCTTCTTCAAGGCCGGTCCGGCGGGCATCCCG
ACCCAGACCGCGTTCAGCCAGAACACCCGCTGGCCGAGCCTGGACGACGACCGCGCCGAGGGCTGCATCCGC
AGCGTCGAGCATGCCTATTCCAAGGAAGGCGGGCTGGCCGTGCTCTACGGCAACATCGCTCTCGACGGCTGC
GTGGTGAAGACCGCCGGGGTCGACGAGTCGATCCACGTGTTCGAAGGCAGCGCGAAGATCTTCGAAAGCCAG
GACGCCGCGGTGAAAGGCATCCTCGGCGACGAGGTGAAGGCCGGCGACATCGTGATCATCCGCTACGAAGGA
CCGAAGGGCGGGCCGGGCATGCAGGAAATGCTCTATCCGACCAGCTACCTGAAGTCCAAGGGCCTGGGCAAG
CAGTGCGCCCTGCTCACCGACGGACGCTTCTCCGGCGGCACCTCGGGCCTGTCCATCGGTCACGCCTCCCCG
GAAGCCGCCGCCGGCGGCGCCATCGGCCTGGTGCAGGACGGCGACAAGGTGCTGATCGACATTCCCAATCGC
TCGATCAACCTGCTGGTCAGCGACGAAGAACTCGCCGCCCGCCGCGCCGAACAGGACAAGAAGGGCTGGAAG
CCGGCCGCGCCGCGTGCCCGCCGCGTCAGCACCGCGCTCAAGGCCTACGCCCTGCTCGCCACCAGCGCCGAC
AAGGGCGCGGTACGCAACAAGGCGCTGCTGGACGGCTGA
Translation:
MPDYRSKTSTHGRNMAGARALWRATGMKDEDFKKPIIAIANSFTQFVPGHVHLKDLGQLVAREIEKAGGVAK
EFNTIAVDDGIAMGHDGMLYSLPSREIIADSVEYMVNAHCADAIVCISNCDKITPGMLMAALRLNIPVVFVS
GGPMEAGKTKLASHGLDLVDAMVVAADDSCSDEKVAEYERSACPTCGSCSGMFTANSMNCLTEALGLSLPGN
GSTLATHADREQLFLRAGRLAVELCQRYYGEGDDSVLPRNIANFKAFENAMTLDIAMGGSTNTILHLLAAAQ
EAEVPFDLRDIDRLSRKVPQLCKVAPNIQKYHMEDVHRAGGIFSILGELARGGLLHTDVPTVHSPSMADAIA
QWDITQTRDEAVHTFFKAGPAGIPTQTAFSQNTRWPSLDDDRAEGCIRSVEHAYSKEGGLAVLYGNIALDGC
VVKTAGVDESIHVFEGSAKIFESQDAAVKGILGDEVKAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLGK
QCALLTDGRFSGGTSGLSIGHASPEAAAGGAIGLVQDGDKVLIDIPNRSINLLVSDEELAARRAEQDKKGWK
PAAPRARRVSTALKAYALLATSADKGAVRNKALLDG*
AnnotationID:468GeneID:459
AnnotatorUID: diggins
Modification Date/Time: 2005-03-24 18:06:20
Gene Name:ilvD
Confidence Code:2
GeneProduct:dihydroxy-acid dehydratase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00920
Secondary Functional Category(ies):amino acid biosynthesis and metabolims
EC Number:4.2.1.9
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70693.1|AE016712_11 (AE016793) dihydroxy-acid dehydratase [Pseudomonas putida KT2440]
          Length = 613

 Score = 1131 bits (2926), Expect = 0.0
 Identities = 563/612 (91%), Positives = 587/612 (95%)

>gb|AAM35237.1| (AE011660) dihydroxy-acid dehydratase [Xanthomonas axonopodis pv.
           citri str. 306]
          Length = 612

 Score = 1073 bits (2776), Expect = 0.0
 Identities = 528/612 (86%), Positives = 571/612 (93%)

>gb|AAN83125.1|AE016769_240 (AE016769) Dihydroxy-acid dehydratase [Escherichia coli CFT073]
          Length = 616

 Score =  917 bits (2370), Expect = 0.0
 Identities = 461/616 (74%), Positives = 521/616 (84%), Gaps = 4/616 (0%)






 
Structural Features:
pfam00920, ILVD_EDD, Dehydratase family.
CD-Length = 531 residues, 100.0% aligned
Score =  750 bits (1937), Expect = 0.0
Identities = 315/577 (54%), Positives = 379/577 (65%), Gaps = 50/577 (8%)








   
Genomic Context:






   
Comment:


















    ReferenceID:921




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18063




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33693




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:459_cdd.html


    		




 
URL:

















    ReferenceID:35408




  
Author/Investigator(s):
        Cox JL, Cox BJ, Fidanza V, Calhoun DH.





  
Title:
        The complete nucleotide sequence of the ilvGMEDA cluster of Escherichia coli K-12.




  
PubMed:
        3315862


MedLine:
        




    
Source:
Gene. 1987;56(2-3):185-98.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3315862

















    ReferenceID:35409




  
Author/Investigator(s):
        Godon JJ, Chopin MC, Ehrlich SD.





  
Title:
        Branched-chain amino acid biosynthesis genes in Lactococcus lactis subsp. lactis.




  
PubMed:
        1400210


MedLine:
        




    
Source:
J Bacteriol. 1992 Oct;174(20):6580-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1400210









           
Homologs By Global Alignment 

           

       



     
Gene ID:459
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
785
                           PA0353_tr
                           translation of PA0353
                           612
                           99.99
                           99.99
                           0
                           3156.0
     

     
     
33964
                           gb|AAG03742.1|AE004473_4
                           (AE004473) dihydroxy-acid dehydratase [Pseudomonas aeruginosa PAO1]
                           612
                           99.99
                           99.99
                           0
                           3156.0
     

     
     
33965
                           gb|AAN70693.1|AE016712_11
                           (AE016793) dihydroxy-acid dehydratase [Pseudomonas putida KT2440]
                           613
                           91.84
                           95.75
                           1
                           2926.0
     

     
     
33966
                           gb|AAO58484.1|
                           (AE016874) dihydroxy-acid dehydratase [Pseudomonas syringae pv. tomato str. DC3000]
                           615
                           90.56
                           95.12
                           3
                           2906.0
     

     
     
33967
                           gb|AAM35237.1|
                           (AE011660) dihydroxy-acid dehydratase [Xanthomonas axonopodis pv. citri str. 306]
                           612
                           86.27
                           93.30
                           0
                           2776.0
     

     






      

          
GC ORFID: 43461How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0353Glimmer Score: 

          
Start: 6213398Stop: 6211560

          
Length: 1839


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0353 translation of PA0353Homolog Bit Score 1220.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3341 (ORF: BLASTX 1124.0), t_PA0353 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCCGATTACCGCTCGAAAACCTCCACCCACGGCCGCAACATGGCCGGCGCCCGCGCCCTCTGGCGCGCC 
ACCGGGATGAAGGACGAAGACTTCAAGAAGCCGATCATCGCCATCGCCAACTCCTTCACCCAGTTCGTGCCC 
GGCCACGTGCACCTGAAGGACCTCGGCCAACTGGTCGCCCGCGAGATCGAGAAGGCCGGCGGCGTGGCCAAG 
GAATTCAACACCATCGCCGTCGACGACGGCATCGCCATGGGCCACGACGGCATGCTCTATTCGCTGCCGAGC 
CGGGAGATCATCGCCGACTCCGTGGAATACATGGTCAACGCCCACTGCGCCGACGCCATCGTCTGCATCTCC 
AACTGCGACAAGATCACCCCCGGCATGCTGATGGCGGCGCTGCGCCTGAACATCCCGGTAGTGTTCGTCTCC 
GGCGGGCCGATGGAAGCCGGCAAGACCAAGCTGGCCAGCCACGGCCTGGACCTGGTCGATGCCATGGTGGTG 
GCCGCCGACGACTCCTGCTCCGACGAGAAGGTCGCCGAGTACGAGCGCAGCGCCTGCCCGACCTGCGGCTCC 
TGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAGGCGCTCGGCCTGTCGCTGCCGGGCAAC 
GGCTCGACCCTGGCCACCCACGCCGACCGCGAGCAACTGTTCCTGCGCGCCGGGCGCCTGGCCGTGGAACTC 
TGCCAGCGCTACTACGGCGAAGGCGACGACAGCGTGCTGCCGCGCAACATCGCCAACTTCAAGGCCTTCGAG 
AACGCCATGACCCTGGACATCGCCATGGGCGGCTCGACCAATACCATCCTGCACCTGCTGGCCGCCGCCCAG 
GAGGCCGAGGTGCCGTTCGACCTGCGCGACATCGACCGCCTGTCACGCAAGGTGCCACAACTGTGCAAGGTG 
GCGCCGAACATCCAGAAGTACCACATGGAAGACGTGCATCGCGCCGGCGGCATCTTCTCCATCCTCGGCGAA 
CTGGCCCGCGGTGGCCTGCTGCACACCGACGTCCCCACCGTGCACAGCCCGAGCATGGCCGACGCCATCGCC 
CAGTGGGACATCACCCAGACCCGCGACGAAGCCGTGCACACCTTCTTCAAGGCCGGTCCGGCGGGCATCCCG 
ACCCAGACCGCGTTCAGCCAGAACACCCGCTGGCCGAGCCTGGACGACGACCGCGCCGAGGGCTGCATCCGC 
AGCGTCGAGCATGCCTATTCCAAGGAAGGCGGGCTGGCCGTGCTCTACGGCAACATCGCTCTCGACGGCTGC 
GTGGTGAAGACCGCCGGGGTCGACGAGTCGATCCACGTGTTCGAAGGCAGCGCGAAGATCTTCGAAAGCCAG 
GACGCCGCGGTGAAAGGCATCCTCGGCGACGAGGTGAAGGCCGGCGACATCGTGATCATCCGCTACGAAGGA 
CCGAAGGGCGGGCCGGGCATGCAGGAAATGCTCTATCCGACCAGCTACCTGAAGTCCAAGGGCCTGGGCAAG 
CAGTGCGCCCTGCTCACCGACGGACGCTTCTCCGGCGGCACCTCGGGCCTGTCCATCGGTCACGCCTCCCCG 
GAAGCCGCCGCCGGCGGCGCCATCGGCCTGGTGCAGGACGGCGACAAGGTGCTGATCGACATTCCCAATCGC 
TCGATCAACCTGCTGGTCAGCGACGAAGAACTCGCCGCCCGCCGCGCCGAACAGGACAAGAAGGGCTGGAAG 
CCGGCCGCGCCGCGTGCCCGCCGCGTCAGCACCGCGCTCAAGGCCTACGCCCTGCTCGCCACCAGCGCCGAC 
AAGGGCGCGGTACGCAACAAGGCGCTGCTGGACGGCTGA