ViewGene

Locus Name:
GeneID:
Location:

GeneID:4561
TrivialName: PA14_45780
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_45780
PAO1 Ortholog Locus:PA1445
Sequence Length:453
Protein Length:150
Start: 4070203
Stop: 4069751
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04834.1|AE004574_5 (AE004574) flagellar protein FliO [Pseudomonas aeruginosa PAO1]
Identities = 150/150 (100%), Positives = 150/150 (100%)
Sequence:
ATGCGTCGCTACCTGTTCGCCGGCTTCCTGCCGGCACTCGCCTCGCTGAGCGCGCCGCTGTGCGCCGCCGAG
GGGACGACGGGTGCCGCCGCGCCGACGGTGGGCGCCGCTTCCGGCGCCGCCGCGCAACTGGCCCAACTGGTG
CTCGGCCTGGGCCTGGTGATCGGCCTGATCTTCCTGCTCGCCTGGCTGGTGCGCCGGGTGCAACAGGCCGGT
CCGCGCGGCAACCGCTTGATCCGCACCCTTGCCAGTCAGCCGCTGGGTCCGCGCGACCGGCTGGTGCTGGTG
CAGGTCGGCGAGGAGCAGATCCTGCTCGGCCTGACGCCCGGCCGGATCACGCCGCTGCACGTGCTCAAGGAG
CCGGTACACCTGCCGGACGGCGAGCCGGCCACGCCGGAATTCGCCCAGCGCCTGCTGGAGCTGCTGAACAAG
GACCCCAAGGGCAAGCCATGA
Translation:
MRRYLFAGFLPALASLSAPLCAAEGTTGAAAPTVGAASGAAAQLAQLVLGLGLVIGLIFLLAWLVRRVQQAG
PRGNRLIRTLASQPLGPRDRLVLVQVGEEQILLGLTPGRITPLHVLKEPVHLPDGEPATPEFAQRLLELLNK
DPKGKP*
AnnotationID:4567GeneID:4561
AnnotatorUID: diggins
Modification Date/Time: 2005-02-24 09:09:13
Gene Name:fliO
Confidence Code:1
GeneProduct:flagellar protein FliO
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(16) Motility & Attachment
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG3190,pfam04347
Secondary Functional Category(ies):chemotaxis
EC Number:
Status:ACTIVE
Pathway:
Homology:
>gb|AAO55489.1| (AE016862) flagellar protein FliO [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 146

 Score =  151 bits (381), Expect = 1e-36
 Identities = 81/150 (54%), Positives = 107/150 (71%), Gaps = 5/150 (3%)

>gb|AAN69934.1|AE016632_5 (AE016790) flagellar assembly protein FliO [Pseudomonas putida
           KT2440]
          Length = 144

 Score =  137 bits (346), Expect = 1e-32
 Identities = 76/139 (54%), Positives = 93/139 (66%), Gaps = 2/139 (1%)

Structural Features:
COG3190, FliO, Flagellar biogenesis protein 
[Cell motility and secretion]
CD-Length = 137 residues,  99.3% aligned
Score =  119 bits (300), Expect = 1e-28
Identities = 49/136 (36%), Positives = 76/136 (55%), Gaps = 6/136 (4%)

pfam04347, FliO, Flagellar biosynthesis protein, FliO.
CD-Length = 86 residues,  96.5% aligned
Score = 96.5 bits (240), Expect = 1e-21
Identities = 50/86 (58%), Positives = 64/86 (74%), Gaps = 4/86 (4%)
Genomic Context:
Comment:
ReferenceID:9123
Author/Investigator(s): GenNotator
Title:
PubMed:
MedLine:
Source:
Reference Type:BLASTP
Data:
URL:
ReferenceID:26265
Author/Investigator(s): GenNotator
Title: CDD Search
PubMed:
MedLine:
Source:
Reference Type:RPSBLAST
Data:
URL:
ReferenceID:37385
Author/Investigator(s):
Title: CDD Search
PubMed:
MedLine:
Source:
Reference Type:Misc (text/plain)
Data:4561_cdd.html
URL:
ReferenceID:37976
Author/Investigator(s): Malakooti J, Ely B, Matsumura P.
Title: Molecular characterization, nucleotide sequence, and expression of the fliO, fliP, fliQ, and fliR genes of Escherichia coli.
PubMed: 8282695
MedLine:
Source:
J Bacteriol. 1994 Jan;176(1):189-97.
Reference Type:Journal
Data:
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8282695
ReferenceID:37977
Author/Investigator(s): Ohnishi K, Fan F, Schoenhals GJ, Kihara M, Macnab RM.
Title: The FliO, FliP, FliQ, and FliR proteins of Salmonella typhimurium: putative components for flagellar assembly.
PubMed: 9324257
MedLine:
Source:
J Bacteriol. 1997 Oct;179(19):6092-9.
Reference Type:Journal
Data:
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9324257
ReferenceID:37983
Author/Investigator(s): Simpson DA, Ramphal R, Lory S.
Title: Characterization of Pseudomonas aeruginosa fliO, a gene involved in flagellar biosynthesis and adherence.
PubMed: 7622217
MedLine:
Source:
nfect Immun. 1995 Aug;63(8):2950-7.
Reference Type:Journal
Data:
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7622217
Homologs By Global Alignment
Gene ID:4561

Identity:

0% 10% 20% 30% 40% 50% 60% 70% 80% 90% 100%
HomologID Accession Description Length PctIdentity PctSimilarity Gaps Score
6926 PA1445_tr translation of PA1445 150 99.99 99.99 0 753.0
184630 gb|AAG04834.1|AE004574_5 (AE004574) flagellar protein FliO [Pseudomonas aeruginosa PAO1] 150 99.99 99.99 0 753.0
184631 gb|AAA79755.1| (L39832) flagellar protein [Pseudomonas aeruginosa] 156 82.69 83.97 21 628.0
184632 gb|AAO55489.1| (AE016862) flagellar protein FliO [Pseudomonas syringae pv. tomato str. DC3000] 151 53.64 70.86 6 388.0
184633 gb|AAN69934.1|AE016632_5 (AE016790) flagellar assembly protein FliO [Pseudomonas putida KT2440] 154 50.64 64.93 14 349.0
GC ORFID: 47563How Found: BLASTX
GC_TrimmedSeqID: 69Blast Result ID
Subject Sequence Name: t_PA1445Glimmer Score:
Start: 883706Stop: 884158
Length: 453
Start Codon: ATGTruncated Start:
Stop Codon: TGATruncated Stop:
Homolog: t_PA1445 translation of PA1445Homolog Bit Score 294.0
Other Homologs: psyr_15may02_Scaffold8_revised_gene7930 (ORF: BLASTX 158.0), t_PA1445 (ORF: GLIMMER)
GC ORF Sequence
ATGCGTCGCTACCTGTTCGCCGGCTTCCTGCCGGCACTCGCCTCGCTGAGCGCGCCGCTGTGCGCCGCCGAG
GGGACGACGGGTGCCGCCGCGCCGACGGTGGGCGCCGCTTCCGGCGCCGCCGCGCAACTGGCCCAACTGGTG
CTCGGCCTGGGCCTGGTGATCGGCCTGATCTTCCTGCTCGCCTGGCTGGTGCGCCGGGTGCAACAGGCCGGT
CCGCGCGGCAACCGCTTGATCCGCACCCTTGCCAGTCAGCCGCTGGGTCCGCGCGACCGGCTGGTGCTGGTG
CAGGTCGGCGAGGAGCAGATCCTGCTCGGCCTGACGCCCGGCCGGATCACGCCGCTGCACGTGCTCAAGGAG
CCGGTACACCTGCCGGACGGCGAGCCGGCCACGCCGGAATTCGCCCAGCGCCTGCTGGAGCTGCTGAACAAG
GACCCCAAGGGCAAGCCATGA