ViewGene
Locus Name:
GeneID:
Location:

GeneID:456
TrivialName: PA14_37560
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_37560
PAO1 Ortholog Locus:PA2084
Sequence Length:1833
Protein Length:610
Start: 3343233
Stop: 3341401
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05472.1|AE004636_3 (AE004636) probable asparagine synthetase [Pseudomonas aeruginosa PAO1]
Identities = 608/610 (99%), Positives = 608/610 (99%)
Sequence:
ATGTGTGGAATCACCGGCTGGACCGACTGGAGCCGCGACCTCGGCGAGCAGCGCAGCATCCTCGAACGCATG
ACCCGTACCCTGGCCCCGCGCGGGCCGGACGCCGAAGGCCTGTGGCTGTCGCGGCACGCGCTGCTCGGCCAT
CGGCGCCTGGCGATCATCGACCTGGAGAACGGCGCCCAACCGATGCTTGCCGAGGCGGGCGGGCGGGTGGCG
CTGACCTACAGCGGCGAGGTCTACAACTTCCGCGAGCTGCGCGCCGAGCTGGAGACGCTGGGCCAGGTCTTC
CGTACCCGCTCGGACACCGAGGTGGTGCTGCGCGCCTATCTGCAATGGGGCGAGGCTTCCTTCGCCCGGCTC
CGCGGCATCTACGGCTTCGCCCTGTGGGACGAGCGCGAGCAGAGCCTGTGGCTGGTGCGCGATCGCTTCGGG
GTCAAGCCGCTGTACTACTACCCGACCGCCGGAGGCGTGCTGTTCGCCTCGGAACCCAAGGCGATCTTCGCC
AACCCCGAGGCCAACCCGGTGCTCGACGCCAGCGGCATCGCCGAGCTGTTCGCCCTGACCACCGCGCCGACT
CCCGGCCACGGCCTGTACAAGGGCCTGCGCCAGGTCCGGCCGGGCCAGGCGCTGCGCTTCGACCGCAATGGT
GTGCGCGAGCTGGTCTACTGGGCGCTGCGCGCGGAACGTCACGAAGAGGGCGCCGAGGACAGCGCCGAACGC
GCCGGCCAGTTGCTCCGCGAGGCAGTGGCCGAGCAGCTGGTGGCCGACGTCCCGCTGGGCAGCCTGCTTTCC
GGCGGGCTCGACTCCAGCGCCATCAGCGCCTTCGCGGTGGCCGCGCTGGATGGCGACGCGCGGCGCCTGCCG
ACCTTCTCCGTGGACTTCCCCGGCGAAGGCCGCGGCTTCGTCCCGACGCCCTGGCAAAGCAGCTGGGACGAA
CCCTATGCGCAGCTGGCGGCGAACTTCCTCGGTACGCCGCACCGTACCGTGCTGGTCGCCCCGGAAGAGGTG
CTGGAACAGGACGAGGCGGTGCTCCAGGCCCGCGACCTGCCCGGCTGGGGCGAGCTGGACGCCTCGCTCTAC
CTGCTGTTCCGCCAGGTCCGCGAGCACACCACGGTGGCGCTGTCGGGCGAGTCGGCCGACGAGGTGTTCGGC
GGCTACCCGTTCTTCCACGACCCGAGCGCCCTCGCACACGACGGCTTCCCCTGGCTGGCCGGCAAGAGCGGG
CCCTGGCAACTGCTGCGGCGGGAGGTCGCCGAACGGGTGGCGGCGCCGGAATACATCCGGGCGCGCTACCGC
GAGGCCCTCGCCGAAGTGCCGCGACTGGATGGCGAGGATGCCGCCCAGCGGCGCCAGCGCGAGGTCGCCTAC
CTGGCGCTGAGCCGCTGGTTGCCGGCGATGCTCGAACGCAAGGACCGGATGAGCATGGCGGTCGGCCTGGAA
GTGCGCGTGCCGTTCTGCGACCACCGCCTGGTGGAGTACGTATGGAACCTGCCCTGGGCGCTGAAGAGCGTC
GCCGGGGAAAGCAAGAGCCTGCTGCGCCGGGCCCTGCGCGGGCACCTGCCGCAGGCGATCCTGGAGCGGCGC
AAGAGCGGTTTCCCGGCCAACCCCGACCCACGTTACCTGGCGCTGCTGCGCGAGCGGGTCGGCCGCCTGCTC
GCCGACGGCCGCTCGCCGCTGTTCGAACTGGTGGACGCCGGGCGGGTCCGCCAGGCTCTCGAACAGGGCACG
CCGCTGCCCAGCCCGCGTGCCTCGGCGAGCCCGACCGCCGGCCTGGCCTACCTGCTGAACCTGGACCGCTGG
CTGACCCGCCATGGGGTCGACATCAGCCTCTGA
Translation:
MCGITGWTDWSRDLGEQRSILERMTRTLAPRGPDAEGLWLSRHALLGHRRLAIIDLENGAQPMLAEAGGRVA
LTYSGEVYNFRELRAELETLGQVFRTRSDTEVVLRAYLQWGEASFARLRGIYGFALWDEREQSLWLVRDRFG
VKPLYYYPTAGGVLFASEPKAIFANPEANPVLDASGIAELFALTTAPTPGHGLYKGLRQVRPGQALRFDRNG
VRELVYWALRAERHEEGAEDSAERAGQLLREAVAEQLVADVPLGSLLSGGLDSSAISAFAVAALDGDARRLP
TFSVDFPGEGRGFVPTPWQSSWDEPYAQLAANFLGTPHRTVLVAPEEVLEQDEAVLQARDLPGWGELDASLY
LLFRQVREHTTVALSGESADEVFGGYPFFHDPSALAHDGFPWLAGKSGPWQLLRREVAERVAAPEYIRARYR
EALAEVPRLDGEDAAQRRQREVAYLALSRWLPAMLERKDRMSMAVGLEVRVPFCDHRLVEYVWNLPWALKSV
AGESKSLLRRALRGHLPQAILERRKSGFPANPDPRYLALLRERVGRLLADGRSPLFELVDAGRVRQALEQGT
PLPSPRASASPTAGLAYLLNLDRWLTRHGVDISL*
AnnotationID:465GeneID:456
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 17:05:50
Gene Name:
Confidence Code:3
GeneProduct:asparagine synthetase, glutamine-hydrolysing
Cell Localization:()
Synonyms:asnB
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0367,cd00712
Secondary Functional Category(ies):
EC Number:6.3.5.4
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAM24074.1| (AE013048) Asparagine synthase (glutamine-hydrolyzing)
           [Thermoanaerobacter tengcongensis]
          Length = 613

 Score =  535 bits (1377), Expect = e-151
 Identities = 263/613 (42%), Positives = 393/613 (64%), Gaps = 6/613 (0%)

>dbj|BAB12569.1| (AB039379) asparagine synthase homolog [Streptomyces aureofaciens]
          Length = 609

 Score =  528 bits (1361), Expect = e-149
 Identities = 301/619 (48%), Positives = 384/619 (62%), Gaps = 19/619 (3%)

>dbj|BAB05227.1| (AP001512) asparagine synthetase [Bacillus halodurans]
          Length = 615

 Score =  508 bits (1308), Expect = e-143
 Identities = 242/613 (39%), Positives = 378/613 (61%), Gaps = 5/613 (0%)








 
Structural Features:
COG0367, AsnB, Asparagine synthase (glutamine-hydrolyzing) 
[Amino acid transport and metabolism]
CD-Length = 542 residues,  95.9% aligned
Score =  431 bits (1110), Expect = 1e-121
Identities = 222/577 (38%), Positives = 289/577 (50%), Gaps = 59/577 (10%)

cd00712, Asn_B, Glutamine amidotransferases class-II (GATase) 
asparagine synthase_B type.
CD-Length = 225 residues,  99.6% aligned
Score =  254 bits (649), Expect = 4e-68
Identities = 112/228 (49%), Positives = 143/228 (62%), Gaps = 6/228 (2%)







   
Genomic Context:






   
Comment:


















    ReferenceID:915




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18057




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32623




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:456_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:456
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
779
                           PA2084_tr
                           translation of PA2084
                           610
                           99.67
                           99.67
                           0
                           3161.0
     

     
     
33931
                           gb|AAG05472.1|AE004636_3
                           (AE004636) probable asparagine synthetase [Pseudomonas aeruginosa PAO1]
                           610
                           99.67
                           99.67
                           0
                           3161.0
     

     
     
33932
                           gb|AAQ59388.1|
                           (AE016916) asparagine synthetase, glutamine-hydrolysing [Chromobacterium violaceum ATCC 12472]
                           625
                           51.20
                           64.32
                           24
                           1532.0
     

     
     
33934
                           dbj|BAB12569.1|
                           (AB039379) asparagine synthase homolog [Streptomyces aureofaciens]
                           622
                           48.71
                           62.05
                           25
                           1387.0
     

     
     
33936
                           gb|AAP25668.1|
                           (AE017029) asparagine synthetase, glutamine-hydrolyzing [Bacillus anthracis str. Ames]
                           611
                           44.02
                           61.53
                           5
                           1351.0
     

     






      

          
GC ORFID: 43458How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2084Glimmer Score: 

          
Start: 1610681Stop: 1612513

          
Length: 1833


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2084 translation of PA2084Homolog Bit Score 1222.0

          
Other Homologs:  t_PA0051 (ORF: BLASTX 474.0), t_PA2084 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTGTGGAATCACCGGCTGGACCGACTGGAGCCGCGACCTCGGCGAGCAGCGCAGCATCCTCGAACGCATG 
ACCCGTACCCTGGCCCCGCGCGGGCCGGACGCCGAAGGCCTGTGGCTGTCGCGGCACGCGCTGCTCGGCCAT 
CGGCGCCTGGCGATCATCGACCTGGAGAACGGCGCCCAACCGATGCTTGCCGAGGCGGGCGGGCGGGTGGCG 
CTGACCTACAGCGGCGAGGTCTACAACTTCCGCGAGCTGCGCGCCGAGCTGGAGACGCTGGGCCAGGTCTTC 
CGTACCCGCTCGGACACCGAGGTGGTGCTGCGCGCCTATCTGCAATGGGGCGAGGCTTCCTTCGCCCGGCTC 
CGCGGCATCTACGGCTTCGCCCTGTGGGACGAGCGCGAGCAGAGCCTGTGGCTGGTGCGCGATCGCTTCGGG 
GTCAAGCCGCTGTACTACTACCCGACCGCCGGAGGCGTGCTGTTCGCCTCGGAACCCAAGGCGATCTTCGCC 
AACCCCGAGGCCAACCCGGTGCTCGACGCCAGCGGCATCGCCGAGCTGTTCGCCCTGACCACCGCGCCGACT 
CCCGGCCACGGCCTGTACAAGGGCCTGCGCCAGGTCCGGCCGGGCCAGGCGCTGCGCTTCGACCGCAATGGT 
GTGCGCGAGCTGGTCTACTGGGCGCTGCGCGCGGAACGTCACGAAGAGGGCGCCGAGGACAGCGCCGAACGC 
GCCGGCCAGTTGCTCCGCGAGGCAGTGGCCGAGCAGCTGGTGGCCGACGTCCCGCTGGGCAGCCTGCTTTCC 
GGCGGGCTCGACTCCAGCGCCATCAGCGCCTTCGCGGTGGCCGCGCTGGATGGCGACGCGCGGCGCCTGCCG 
ACCTTCTCCGTGGACTTCCCCGGCGAAGGCCGCGGCTTCGTCCCGACGCCCTGGCAAAGCAGCTGGGACGAA 
CCCTATGCGCAGCTGGCGGCGAACTTCCTCGGTACGCCGCACCGTACCGTGCTGGTCGCCCCGGAAGAGGTG 
CTGGAACAGGACGAGGCGGTGCTCCAGGCCCGCGACCTGCCCGGCTGGGGCGAGCTGGACGCCTCGCTCTAC 
CTGCTGTTCCGCCAGGTCCGCGAGCACACCACGGTGGCGCTGTCGGGCGAGTCGGCCGACGAGGTGTTCGGC 
GGCTACCCGTTCTTCCACGACCCGAGCGCCCTCGCACACGACGGCTTCCCCTGGCTGGCCGGCAAGAGCGGG 
CCCTGGCAACTGCTGCGGCGGGAGGTCGCCGAACGGGTGGCGGCGCCGGAATACATCCGGGCGCGCTACCGC 
GAGGCCCTCGCCGAAGTGCCGCGACTGGATGGCGAGGATGCCGCCCAGCGGCGCCAGCGCGAGGTCGCCTAC 
CTGGCGCTGAGCCGCTGGTTGCCGGCGATGCTCGAACGCAAGGACCGGATGAGCATGGCGGTCGGCCTGGAA 
GTGCGCGTGCCGTTCTGCGACCACCGCCTGGTGGAGTACGTATGGAACCTGCCCTGGGCGCTGAAGAGCGTC 
GCCGGGGAAAGCAAGAGCCTGCTGCGCCGGGCCCTGCGCGGGCACCTGCCGCAGGCGATCCTGGAGCGGCGC 
AAGAGCGGTTTCCCGGCCAACCCCGACCCACGTTACCTGGCGCTGCTGCGCGAGCGGGTCGGCCGCCTGCTC 
GCCGACGGCCGCTCGCCGCTGTTCGAACTGGTGGACGCCGGGCGGGTCCGCCAGGCTCTCGAACAGGGCACG 
CCGCTGCCCAGCCCGCGTGCCTCGGCGAGCCCGACCGCCGGCCTGGCCTACCTGCTGAACCTGGACCGCTGG 
CTGACCCGCCATGGGGTCGACATCAGCCTCTGA