ViewGene
Locus Name:
GeneID:
Location:

GeneID:4557
TrivialName: PA14_64090
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_64090
PAO1 Ortholog Locus:PA4846
Sequence Length:444
Protein Length:147
Start: 5712489
Stop: 5712932
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08231.1|AE004898_2 (AE004898) 3-dehydroquinate dehydratase [Pseudomonas aeruginosa PAO1]
Identities = 147/147 (100%), Positives = 147/147 (100%)
Sequence:
ATGGCGACCCTCCTCGTATTGCACGGGCCGAATCTGAACCTGCTGGGCACCCGCGAGCCCGGCACCTACGGT
TCGACCACCCTCGGGCAGATCAACCAGGACCTCGAGCGCCGCGCCCGCGAAGCCGGCCACCACCTGCTGCAT
CTGCAAAGCAACGCCGAATACGAACTGATCGACCGGATCCATGCCGCGCGCGACGAAGGCGTGGACTTCATC
ATCATCAATCCGGCGGCATTCACCCATACCAGCGTCGCGTTACGTGACGCGCTGCTTGCGGTGAGCATCCCA
TTCATCGAAGTGCACCTGTCGAACGTGCACAAACGTGAACCTTTCCGGCATCACTCCTACTTCTCCGACGTG
GCGGTAGGGGTGATCTGCGGTCTCGGCGCCACAGGCTACCGCCTGGCCCTGGAATCCGCCCTTGAACAACTT
CAACGCCCCTGA
Translation:
MATLLVLHGPNLNLLGTREPGTYGSTTLGQINQDLERRAREAGHHLLHLQSNAEYELIDRIHAARDEGVDFI
IINPAAFTHTSVALRDALLAVSIPFIEVHLSNVHKREPFRHHSYFSDVAVGVICGLGATGYRLALESALEQL
QRP*
AnnotationID:4563GeneID:4557
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 06:06:39
Gene Name:aroQ1
Confidence Code:2
GeneProduct:3-dehydroquinate dehydratase
Cell Localization:()
Synonyms:aroQ
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:catabolic dehydroquinase
Functional Category Confidence Code:5
COGs:COG0757,pfam01220
Secondary Functional Category(ies):
EC Number:4.2.1.10
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66187.1|AE016246_4 (AE016776) 3-dehydroquinate dehydratase, type II [Pseudomonas
           putida KT2440]
          Length = 151

 Score =  270 bits (690), Expect = 2e-72
 Identities = 134/144 (93%), Positives = 140/144 (97%)

>gb|AAO58288.1| (AE016873) 3-dehydroquinate dehydratase, type II [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 150

 Score =  264 bits (675), Expect = 1e-70
 Identities = 128/144 (88%), Positives = 139/144 (96%)

>gb|AAM39834.1| (AE012149) catabolic dehydroquinase [Xanthomonas campestris pv.
           campestris str. ATCC 33913]
          Length = 148

 Score =  206 bits (523), Expect = 4e-53
 Identities = 101/146 (69%), Positives = 118/146 (80%)








 
Structural Features:
COG0757, AroQ, 3-dehydroquinate dehydratase II 
[Amino acid transport and metabolism]
CD-Length = 146 residues,  98.6% aligned
Score =  223 bits (569), Expect = 9e-60
Identities = 92/145 (63%), Positives = 105/145 (72%), Gaps = 1/145 (0%)

pfam01220, Dehydroquinase class II
CD-Length = 144 residues,  97.9% aligned
Score =  192 bits (489), Expect = 2e-50
Identities = 90/142 (63%), Positives = 104/142 (73%), Gaps = 1/142 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:9115




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:26257




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35188




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4557_cdd.html


    		




 
URL:

















    ReferenceID:35979




  
Author/Investigator(s):
        Bottomley JR, Clayton CL, Chalk PA, Kleanthous C.





  
Title:
        Cloning, sequencing, expression, purification and preliminary characterization of a type II dehydroquinase from Helicobacter pylori.




  
PubMed:
        8912695


MedLine:
        




    
Source:
Biochem J. 1996 Oct 15;319 ( Pt 2):559-65.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8912695

















    ReferenceID:35980




  
Author/Investigator(s):
        Garbe T, Servos S, Hawkins A, Dimitriadis G, Young D, Dougan G, Charles I.





  
Title:
        The Mycobacterium tuberculosis shikimate pathway genes: evolutionary relationship between biosynthetic and catabolic 3-dehydroquinases.




  
PubMed:
        1910148


MedLine:
        




    
Source:
Mol Gen Genet. 1991 Sep;228(3):385-92.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1910148

















    ReferenceID:35981




  
Author/Investigator(s):
        Calhoun DH, Bonner CA, Gu W, Xie G, Jensen RA.





  
Title:
        The emerging periplasm-localized subclass of AroQ chorismate mutases, exemplified by those from Salmonella typhimurium and Pseudomonas aeruginosa.




  
PubMed:
        11532214


MedLine:
        




    
Source:
Genome Biol. 2001;2(8):RESEARCH0030. Epub 2001 Jul 27.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=11532214









           
Homologs By Global Alignment 

           

       



     
Gene ID:4557
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6921
                           PA4846_tr
                           translation of PA4846
                           147
                           99.99
                           99.99
                           0
                           755.0
     

     
     
184506
                           gb|AAG08231.1|AE004898_2
                           (AE004898) 3-dehydroquinate dehydratase [Pseudomonas aeruginosa PAO1]
                           147
                           99.99
                           99.99
                           0
                           755.0
     

     
     
184507
                           gb|AAB84085.1|
                           (AF010322) catabolic dehydroquinase [Pseudomonas aeruginosa]
                           147
                           99.99
                           99.99
                           0
                           755.0
     

     
     
184508
                           gb|AAN66187.1|AE016246_4
                           (AE016776) 3-dehydroquinate dehydratase, type II [Pseudomonas putida KT2440]
                           151
                           89.40
                           94.03
                           4
                           686.0
     

     
     
184509
                           gb|AAO58288.1|
                           (AE016873) 3-dehydroquinate dehydratase, type II [Pseudomonas syringae pv. tomato str. DC3000]
                           150
                           86.00
                           94.00
                           3
                           672.0
     

     






      

          
GC ORFID: 47559How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4846Glimmer Score: 

          
Start: 4976169Stop: 4976612

          
Length: 444


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4846 translation of PA4846Homolog Bit Score 295.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5768 (ORF: BLASTX 264.0), t_PA0245 (ORF: BLASTX 167.0), t_PA4846 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCGACCCTCCTCGTATTGCACGGGCCGAATCTGAACCTGCTGGGCACCCGCGAGCCCGGCACCTACGGT 
TCGACCACCCTCGGGCAGATCAACCAGGACCTCGAGCGCCGCGCCCGCGAAGCCGGCCACCACCTGCTGCAT 
CTGCAAAGCAACGCCGAATACGAACTGATCGACCGGATCCATGCCGCGCGCGACGAAGGCGTGGACTTCATC 
ATCATCAATCCGGCGGCATTCACCCATACCAGCGTCGCGTTACGTGACGCGCTGCTTGCGGTGAGCATCCCA 
TTCATCGAAGTGCACCTGTCGAACGTGCACAAACGTGAACCTTTCCGGCATCACTCCTACTTCTCCGACGTG 
GCGGTAGGGGTGATCTGCGGTCTCGGCGCCACAGGCTACCGCCTGGCCCTGGAATCCGCCCTTGAACAACTT 
CAACGCCCCTGA