ViewGene
Locus Name:
GeneID:
Location:

GeneID:4554
TrivialName: PA14_23680
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_23680
PAO1 Ortholog Locus:PA3126
Sequence Length:450
Protein Length:149
Start: 2059122
Stop: 2059571
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06514.1|AE004736_11 (AE004736) heat-shock protein IbpA [Pseudomonas aeruginosa PAO1]
Identities = 149/149 (100%), Positives = 149/149 (100%)
Sequence:
ATGAGCAACGCTTTTTCCCTCGCCCCGCTGTTCCGTCATTCCGTAGGCTTCGATCGCTTCAACGATCTGTTC
GAGTCGGCCCTGCGCAATGAGGCCGGGAGTACCTACCCGCCCTACAACGTCGAAAAGCACGGTGACGACGAG
TATCGCATCGTCATCGCCGCCGCCGGCTTCCAGGAAGAAGACCTGGACCTGCAGGTCGAGCGCGGCGTGCTG
ACCGTCAGTGGCGGCAAGCGCGAGAAGTCCACGGACAACGTGACCTACCTGCACCAGGGCATCGCCCAGCGC
GCCTTCAAGCTGTCGTTCCGCCTCGCCGACCATATCGAGGTCAAGGCGGCTTCGCTAGCCAACGGCCTGCTG
AACATCGACCTGGTCCGGCTGGTTCCGGAAGAAGCCAAGCCGAAGCGTATCGCCATCAATGGCCAGCGCCCG
GCACTGGACAACCAGTAA
Translation:
MSNAFSLAPLFRHSVGFDRFNDLFESALRNEAGSTYPPYNVEKHGDDEYRIVIAAAGFQEEDLDLQVERGVL
TVSGGKREKSTDNVTYLHQGIAQRAFKLSFRLADHIEVKAASLANGLLNIDLVRLVPEEAKPKRIAINGQRP
ALDNQ*
AnnotationID:4560GeneID:4554
AnnotatorUID: diggins
Modification Date/Time: 2005-04-13 08:08:07
Gene Name:ibpA
Confidence Code:2
GeneProduct:putative small heat shock protein
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(9) Chaperones & heat shock proteins
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0071,pfam00011
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67597.1|AE016389_5 (AE016781) heat-shock protein IbpA [Pseudomonas putida KT2440]
          Length = 149

 Score =  254 bits (650), Expect = 8e-68
 Identities = 125/147 (85%), Positives = 137/147 (93%)

>gb|AAN55317.1|AE015669_5 (AE015669) 16 kDa heat shock protein A [Shewanella oneidensis MR-1]
          Length = 147

 Score =  137 bits (346), Expect = 1e-32
 Identities = 72/145 (49%), Positives = 95/145 (65%), Gaps = 2/145 (1%)

>gb|AAM87645.1|AE014012_2 (AE014012) heat shock protein [Yersinia pestis KIM]
          Length = 137

 Score =  130 bits (328), Expect = 2e-30
 Identities = 67/133 (50%), Positives = 92/133 (69%), Gaps = 2/133 (1%)






 
Structural Features:
COG0071, IbpA, Molecular chaperone (small heat shock protein) 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 146 residues,  96.6% aligned
Score =  117 bits (295), Expect = 5e-28
Identities = 54/143 (37%), Positives = 75/143 (52%), Gaps = 5/143 (3%)

pfam00011, HSP20, Hsp20/alpha crystallin family.
CD-Length = 102 residues,  99.0% aligned
Score = 65.2 bits (159), Expect = 3e-12
Identities = 30/102 (29%), Positives = 51/102 (50%), Gaps = 4/102 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:9109




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:26251




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32395




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4554_cdd.html


    		




 
URL:

















    ReferenceID:33920




  
Author/Investigator(s):
        Kuczynska-Wisnik D, Kedzierska S, Matuszewska E, Lund P, Taylor A, Lipinska B, Laskowska E.





  
Title:
        The Escherichia coli small heat-shock proteins IbpA and IbpB prevent the aggregation of endogenous proteins denatured in vivo during extreme heat shock.




  
PubMed:
        12055295


MedLine:
        




    
Source:
Microbiology. 2002 Jun;148(Pt 6):1757-65.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12055295

















    ReferenceID:33921




  
Author/Investigator(s):
        Kitagawa M, Matsumura Y, Tsuchido T.





  
Title:
        Small heat shock proteins, IbpA and IbpB, are involved in resistances to heat and superoxide stresses in Escherichia coli.




  
PubMed:
        10713416


MedLine:
        




    
Source:
FEMS Microbiol Lett. 2000 Mar 15;184(2):165-71.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10713416

















    ReferenceID:33922




  
Author/Investigator(s):
        Thomas JG, Baneyx F.





  
Title:
        Roles of the Escherichia coli small heat shock proteins IbpA and IbpB in thermal stress management: comparison with ClpA, ClpB, and HtpG In vivo.




  
PubMed:
        9748451


MedLine:
        




    
Source:
J Bacteriol. 1998 Oct;180(19):5165-72.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9748451

















    ReferenceID:33923




  
Author/Investigator(s):
        Laskowska E, Wawrzynow A, Taylor A.





  
Title:
        IbpA and IbpB, the new heat-shock proteins, bind to endogenous Escherichia coli proteins aggregated intracellularly by heat shock.




  
PubMed:
        8818220


MedLine:
        




    
Source:
Biochimie. 1996;78(2):117-22.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8818220

















    ReferenceID:33924




  
Author/Investigator(s):
        Chuang SE, Burland V, Plunkett G 3rd, Daniels DL, Blattner FR.





  
Title:
        Sequence analysis of four new heat-shock genes constituting the hslTS/ibpAB and hslVU operons in Escherichia coli.




  
PubMed:
        8244018


MedLine:
        




    
Source:
Gene. 1993 Nov 30;134(1):1-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8244018

















    ReferenceID:33925




  
Author/Investigator(s):
        Allen SP, Polazzi JO, Gierse JK, Easton AM.





  
Title:
        Two novel heat shock genes encoding proteins produced in response to heterologous protein expression in Escherichia coli.




  
PubMed:
        1356969


MedLine:
        




    
Source:
J Bacteriol. 1992 Nov;174(21):6938-47.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1356969









           
Homologs By Global Alignment 

           

       



     
Gene ID:4554
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6918
                           PA3126_tr
                           translation of PA3126
                           149
                           99.99
                           99.99
                           0
                           756.0
     

     
     
184436
                           gb|AAG06514.1|AE004736_11
                           (AE004736) heat-shock protein IbpA [Pseudomonas aeruginosa PAO1]
                           149
                           99.99
                           99.99
                           0
                           756.0
     

     
     
184437
                           gb|AAR19314.1|
                           (AY426537) putative heat-shock protein [symbiont bacterium of Paederus fuscipes]
                           149
                           89.26
                           94.63
                           0
                           677.0
     

     
     
184438
                           gb|AAN67597.1|AE016389_5
                           (AE016781) heat-shock protein IbpA [Pseudomonas putida KT2440]
                           151
                           82.78
                           90.72
                           4
                           650.0
     

     
     
184440
                           gb|AAO55687.1|
                           (AE016863) heat shock protein, Hsp20 family [Pseudomonas syringae pv. tomato str. DC3000]
                           149
                           76.51
                           85.90
                           2
                           575.0
     

     






      

          
GC ORFID: 47556How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3126Glimmer Score: 

          
Start: 2894809Stop: 2894360

          
Length: 450


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA3126 translation of PA3126Homolog Bit Score 295.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene966 (ORF: BLASTX 217.0), t_PA3126 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCAACGCTTTTTCCCTCGCCCCGCTGTTCCGTCATTCCGTAGGCTTCGATCGCTTCAACGATCTGTTC 
GAGTCGGCCCTGCGCAATGAGGCCGGGAGTACCTACCCGCCCTACAACGTCGAAAAGCACGGTGACGACGAG 
TATCGCATCGTCATCGCCGCCGCCGGCTTCCAGGAAGAAGACCTGGACCTGCAGGTCGAGCGCGGCGTGCTG 
ACCGTCAGTGGCGGCAAGCGCGAGAAGTCCACGGACAACGTGACCTACCTGCACCAGGGCATCGCCCAGCGC 
GCCTTCAAGCTGTCGTTCCGCCTCGCCGACCATATCGAGGTCAAGGCGGCTTCGCTAGCCAACGGCCTGCTG 
AACATCGACCTGGTCCGGCTGGTTCCGGAAGAAGCCAAGCCGAAGCGTATCGCCATCAATGGCCAGCGCCCG 
GCACTGGACAACCAGTAA