ViewGene
Locus Name:
GeneID:
Location:

GeneID:4551
TrivialName: PA14_65000
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_65000
PAO1 Ortholog Locus:PA4922
Sequence Length:447
Protein Length:148
Start: 5793479
Stop: 5793033
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08307.1|AE004905_5 (AE004905) azurin precursor [Pseudomonas aeruginosa PAO1]
Identities = 147/148 (99%), Positives = 148/148 (100%)
Sequence:
ATGCTACGTAAACTCGCTGCGGTATCCCTGCTGTCCCTGCTCAGTGCGCCGCTGCTGGCTGCCGAGTGCTCG
GTGGACATCCAGGGTAACGACCAGATGCAGTTCAACACCAATGCCATCACCGTCGACAAGAGCTGCAAGCAG
TTCACCGTCAACCTGTCCCACCCCGGCAACCTGCCGAAGAACGTCATGGGCCACAACTGGGTACTGAGCACC
GCCGCCGACATGCAGGGCGTGGTCACCGACGGCATGGCTTCCGGCCTGGACAAGGATTACCTGAAGCCCGAC
GACAGCCGTGTCATCTCCCACACCAAGCTGATCGGCTCGGGCGAGAAGGACTCGGTGACCTTCGACGTCTCC
AAGCTGAAGGAAGGCGAGCAGTACATGTTCTTCTGCACCTTCCCGGGCCACTCCGCGCTGATGAAGGGCACG
CTGACCCTGAAGTGA
Translation:
MLRKLAAVSLLSLLSAPLLAAECSVDIQGNDQMQFNTNAITVDKSCKQFTVNLSHPGNLPKNVMGHNWVLST
AADMQGVVTDGMASGLDKDYLKPDDSRVISHTKLIGSGEKDSVTFDVSKLKEGEQYMFFCTFPGHSALMKGT
LTLK*
AnnotationID:4557GeneID:4551
AnnotatorUID: diggins
Modification Date/Time: 2005-03-13 11:11:04
Gene Name:azu
Confidence Code:1
GeneProduct:azurin precursor
Cell Localization:()
Synonyms:az1
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00127
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAP03090.1| (AY238602) azurin [Burkholderia cepacia]
          Length = 148

 Score =  263 bits (672), Expect = 2e-70
 Identities = 131/148 (88%), Positives = 135/148 (91%)

>emb|CAD24027.1| (AJ431208) azurin I [Achromobacter xylosoxidans]
          Length = 150

 Score =  218 bits (554), Expect = 1e-56
 Identities = 103/147 (70%), Positives = 120/147 (81%)

>emb|CAD24027.1| (AJ431208) azurin I [Achromobacter xylosoxidans]
          Length = 150

 Score =  218 bits (554), Expect = 1e-56
 Identities = 103/147 (70%), Positives = 120/147 (81%)

>gb|AAG27699.1|AF299296_2 (AF299296) azurin precursor [Pseudomonas putida]
          Length = 148

 Score =  207 bits (526), Expect = 2e-53
 Identities = 98/148 (66%), Positives = 118/148 (79%)






 
Structural Features:
pfam00127, Copper-bind, Copper binding proteins, plastocyanin/azurin family.
CD-Length = 99 residues, 100.0% aligned
Score = 97.3 bits (242), Expect = 6e-22
Identities = 44/128 (34%), Positives = 53/128 (41%), Gaps = 29/128 (22%)






   
Genomic Context:






   
Comment:


















    ReferenceID:9103




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:26245




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35134




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4551_cdd.html


    		




 
URL:

















    ReferenceID:35925




  
Author/Investigator(s):
        van de Kamp M, Hali FC, Rosato N, Agro AF, Canters GW.





  
Title:
        Purification and characterization of a non-reconstitutable azurin, obtained by heterologous expression of the Pseudomonas aeruginosa azu gene in Escherichia coli.




  
PubMed:
        2119806


MedLine:
        




    
Source:
Biochim Biophys Acta. 1990 Sep 19;1019(3):283-92.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2119806









           
Homologs By Global Alignment 

           

       



     
Gene ID:4551
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6915
                           PA4922_tr
                           translation of PA4922
                           148
                           99.32
                           99.99
                           0
                           759.0
     

     
     
184357
                           gb|AAG08307.1|AE004905_5
                           (AE004905) azurin precursor [Pseudomonas aeruginosa PAO1]
                           148
                           99.32
                           99.99
                           0
                           759.0
     

     
     
184358
                           emb|CAA30279.1|
                           (X07317) unnamed protein product [Pseudomonas aeruginosa]
                           148
                           99.32
                           99.99
                           0
                           759.0
     

     
     
184359
                           gb|AAA25730.1|
                           (M30389) azurin [Pseudomonas aeruginosa]
                           148
                           99.32
                           99.99
                           0
                           759.0
     

     
     
184360
                           gb|AAP03090.1|
                           (AY238602) azurin [Burkholderia cepacia]
                           153
                           86.92
                           91.50
                           10
                           676.0
     

     






      

          
GC ORFID: 47553How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4922Glimmer Score: 

          
Start: 5057160Stop: 5056714

          
Length: 447


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4922 translation of PA4922Homolog Bit Score 296.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3449 (ORF: BLASTX 227.0), t_PA4922 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCTACGTAAACTCGCTGCGGTATCCCTGCTGTCCCTGCTCAGTGCGCCGCTGCTGGCTGCCGAGTGCTCG 
GTGGACATCCAGGGTAACGACCAGATGCAGTTCAACACCAATGCCATCACCGTCGACAAGAGCTGCAAGCAG 
TTCACCGTCAACCTGTCCCACCCCGGCAACCTGCCGAAGAACGTCATGGGCCACAACTGGGTACTGAGCACC 
GCCGCCGACATGCAGGGCGTGGTCACCGACGGCATGGCTTCCGGCCTGGACAAGGATTACCTGAAGCCCGAC 
GACAGCCGTGTCATCTCCCACACCAAGCTGATCGGCTCGGGCGAGAAGGACTCGGTGACCTTCGACGTCTCC 
AAGCTGAAGGAAGGCGAGCAGTACATGTTCTTCTGCACCTTCCCGGGCCACTCCGCGCTGATGAAGGGCACG 
CTGACCCTGAAGTGA