ViewGene
Locus Name:
GeneID:
Location:

GeneID:4544
TrivialName: PA14_03030
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_03030
PAO1 Ortholog Locus:PA0245
Sequence Length:447
Protein Length:148
Start: 267579
Stop: 267133
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03634.1|AE004462_7 (AE004462) 3-dehydroquinate dehydratase [Pseudomonas aeruginosa PAO1]
Identities = 147/148 (99%), Positives = 147/148 (99%)
Sequence:
ATGACGCGCACGGTACTGGTCCTGAACGGCCCCAACCTGAATCTGCTCGGCATCCGCGAGCCACAGACCTAC
GGCCGGCGGACCCTGGGCGAAATCGCCGACGAATGCGCGGCCTTCGCCGAGACGCGCGGCTTCGCCGTGGAC
TTCCGCCAGACCAACCAGGAAGGCCAACTGCTCGACTGGATCCACCAGGCCCGCGGCCGCGTCGCCGGCATC
GTCATCAACCCCGCCGCCTGGACCCACACCTCGGTGGCGCTGCGCGACGCCCTGGCCGCGGTGGAACTGCCG
GTGGTCGAAGTGCACCTGTCCAACGTCCACCAGCGCGAAGCGTTCCGCCACCACTCCTACGTCTCGCCGGTG
GCGCTGGGGGTGATCTGCGGATTCGGCAGCCTCGGCTACCGCCTGGCCCTGGAACATTTCGCCGAGCGTTTC
GAGGCGGCCGCATGA
Translation:
MTRTVLVLNGPNLNLLGIREPQTYGRRTLGEIADECAAFAETRGFAVDFRQTNQEGQLLDWIHQARGRVAGI
VINPAAWTHTSVALRDALAAVELPVVEVHLSNVHQREAFRHHSYVSPVALGVICGFGSLGYRLALEHFAERF
EAAA*
AnnotationID:4550GeneID:4544
AnnotatorUID: diggins
Modification Date/Time: 2005-03-23 19:07:16
Gene Name:aroQ2
Confidence Code:2
GeneProduct:3-dehydroquinate dehydratase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:dehydroquinase
Functional Category Confidence Code:5
COGs:COG0757,pfam01220
Secondary Functional Category(ies):
EC Number:4.2.1.10
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN68019.1|AE016434_10 (AE016783) 3-dehydroquinate dehydratase, type II [Pseudomonas
           putida KT2440]
          Length = 149

 Score =  207 bits (527), Expect = 1e-53
 Identities = 98/148 (66%), Positives = 114/148 (77%)

>gb|AAL45325.1| (AE009381) 3-dehydroquinate dehydratase [Agrobacterium tumefaciens
           str. C58 (U. Washington)]
          Length = 147

 Score =  173 bits (438), Expect = 3e-43
 Identities = 82/142 (57%), Positives = 104/142 (73%)






 
Structural Features:
COG0757, AroQ, 3-dehydroquinate dehydratase II [Amino acid transport and metabolism]
CD-Length = 146 residues,  99.3% aligned
Score =  232 bits (592), Expect = 2e-62
Identities = 89/145 (61%), Positives = 103/145 (71%)

pfam01220, DHquinase_II, Dehydroquinase class II.
CD-Length = 144 residues,  98.6% aligned
Score =  192 bits (490), Expect = 1e-50
Identities = 80/142 (56%), Positives = 101/142 (71%)






   
Genomic Context:






   
Comment:


















    ReferenceID:9089




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:26231




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33604




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4544_cdd.html


    		




 
URL:

















    ReferenceID:35452




  
Author/Investigator(s):
        Bottomley JR, Clayton CL, Chalk PA, Kleanthous C.





  
Title:
        Cloning, sequencing, expression, purification and prelimina




  
PubMed:
        8912695


MedLine:
        




    
Source:
Biochem J. 1996 Oct 15;319 ( Pt 2):559-65.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8912695









           
Homologs By Global Alignment 

           

       



     
Gene ID:4544
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6906
                           PA0245_tr
                           translation of PA0245
                           148
                           99.32
                           99.32
                           0
                           760.0
     

     
     
184181
                           gb|AAG03634.1|AE004462_7
                           (AE004462) 3-dehydroquinate dehydratase [Pseudomonas aeruginosa PAO1]
                           148
                           99.32
                           99.32
                           0
                           760.0
     

     
     
184182
                           gb|AAN68019.1|AE016434_10
                           (AE016783) 3-dehydroquinate dehydratase, type II [Pseudomonas putida KT2440]
                           149
                           65.77
                           76.51
                           1
                           527.0
     

     
     
6907
                           PA4846_tr
                           translation of PA4846
                           149
                           58.38
                           69.79
                           3
                           414.0
     

     
     
184196
                           gb|AAB84085.1|
                           (AF010322) catabolic dehydroquinase [Pseudomonas aeruginosa]
                           149
                           58.38
                           69.79
                           3
                           414.0
     

     






      

          
GC ORFID: 47546How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0245Glimmer Score: 

          
Start: 6068920Stop: 6068474

          
Length: 447


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0245 translation of PA0245Homolog Bit Score 297.0

          
Other Homologs:  t_PA4846 (ORF: BLASTX 164.0), psyr_15may02_Scaffold4_revised_gene5768 (ORF: BLASTX 162.0), t_PA0245 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACGCGCACGGTACTGGTCCTGAACGGCCCCAACCTGAATCTGCTCGGCATCCGCGAGCCACAGACCTAC 
GGCCGGCGGACCCTGGGCGAAATCGCCGACGAATGCGCGGCCTTCGCCGAGACGCGCGGCTTCGCCGTGGAC 
TTCCGCCAGACCAACCAGGAAGGCCAACTGCTCGACTGGATCCACCAGGCCCGCGGCCGCGTCGCCGGCATC 
GTCATCAACCCCGCCGCCTGGACCCACACCTCGGTGGCGCTGCGCGACGCCCTGGCCGCGGTGGAACTGCCG 
GTGGTCGAAGTGCACCTGTCCAACGTCCACCAGCGCGAAGCGTTCCGCCACCACTCCTACGTCTCGCCGGTG 
GCGCTGGGGGTGATCTGCGGATTCGGCAGCCTCGGCTACCGCCTGGCCCTGGAACATTTCGCCGAGCGTTTC 
GAGGCGGCCGCATGA