ViewGene
Locus Name:
GeneID:
Location:

GeneID:454
TrivialName: PA14_29880
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_29880
PAO1 Ortholog Locus:PA2647
Sequence Length:1848
Protein Length:615
Start: 2589052
Stop: 2587205
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06035.1|AE004693_6 (AE004693) NADH dehydrogenase I chain L [Pseudomonas aeruginosa PAO1]
Identities = 612/615 (99%), Positives = 614/615 (99%)
Sequence:
ATGAACCTTCTACCTCTTACCTTCCTGTTCCCCCTGGTCGGCTTCCTGCTGCTGTCGTTCTCCCGCGGCAGG
TGGTCGGAAAACCTTTCGGCGCTGGTCGGCGTCGGCTCGGTCGGCCTCTCCGCCCTGAGCGCGGCCTGGGCC
ATCTTCAGCTTCCACAGCAGTCCGCCGGAAGGCGGCGCCTACAGCCTGGTGCTGTGGCAGTGGATGGCGGCC
GGCGACTTCAGCAGCAACTTCACCCTGTACCTGGACGGCCTCTCGGTAACCATGCTCGGCGTGGTTACCGGC
GTCGGCTTCCTGATCCACCTGTTCGCCTCCTGGTACATGCGTGGCGAAACCGGCTATTCGCGCTTCTTCGCC
TACACCAACCTGTTCATTGCCAGCATGCTGTTCCTGGTGCTGGGCGACAACCTGCTGTTCCTCTACTTCGGC
TGGGAAGGCGTGGGCCTGTGCAGCTACCTGCTGATCGGCTTCTACTACAGCAATCGCAACAACGGTAACGCG
GCGCTCAAGGCATTCATCGTCACCCGCGTCGGCGACGTGTTCATGGCTTTCGGCCTGTTCATCCTGTTCCAG
CAGTTCGGCACCCTGAACATCCAGGAGCTGCTGGTGCTGGCGCCGCAGAAGTTCCCGGAAGGCAACCTCTGG
CTGACCCTGGCGACCCTGGCCCTGCTCGGCGGCGCGGTCGGCAAGTCCGCCCAACTGCCGCTGCAGACCTGG
CTGGCCGACGCCATGGCCGGCCCGACCCCGGTCTCCGCGCTGATCCACGCCGCCACCATGGTGACCGCCGGC
GTCTACCTGATCGCCCGTTGCCACGGCCTGTTCACCCTGGCCCCGGACATCCTCGAACTGGTCGGCATCGTC
GGCGCGGTGACCCTGGTCCTGGCCGGCTTCGCCGCCCTGGTGCAGACCGATATCAAGCGCATCCTCGCCTAC
TCGACCATGAGCCAGATCGGCTACATGTTCCTCGCCCTCGGCGTCGGCGCCTGGGACGCGGCGATCTTCCAC
CTGATGACCCACGCCTTCTTCAAGGCCCTGCTGTTCCTCGCCTCGGGCGCGGTGATCATCGCCTGCCACCAC
GAGCAGAACATCTTCAAGATGGGCGGCTTGTGGAAGAAGCTGCCGCTGGCCTACGCCAGCTTCGTGGTCGGC
GGTGCGGCGCTGGCCGCCCTGCCCTTCCTCACCGCGGGCTTCTACTCCAAGGACGAGATCCTCTGGGAGGCC
TTCGCCAGCGGCCATCGCGAGTTGCTGATCGCCGGCCTGGTGGGTGCGTTCCTGACCTCGATCTACACCTTC
CGCCTGATCTTCGTCGCCTTCCACGGCGAGCCGAAGACCGAAGCCCACGCCGGCCACGGGATTTCCCACTGG
CTGCCGCTCAGCGTGCTGATCGTCCTGTCGACCTTCGTCGGCGCCCTGATCACCCCGCCGCTGGCCGGCGTC
CTGCCGGAAAGCGTCGGCCACGCCGGTGGCGAAGCCAAGCACAGCCTGGAACTGGCCTCGGGCGCCATCGCC
ATCGCCGGCATCCTGCTGGCCGGCCTGCTGTTCCTCGGCCAGCGCCGCTTCGTCAGCGCCCTGGCGAAGAGC
GCCCCGGGTCGCTTCTTCGGTACCTGGTGGTACCACGCCTGGGGCTTCGACTGGCTGTACGACAAGCTGTTC
GTGAAACCCTACCTGCTGCTCTGCCAACTGCTGGGGCGCGACCCCATCGACCGTACCCTGGGCGTCGTTCCG
TTCAGCGTGCGCGGCGGACACAACCTCCTCAGCCTCACCGAGAACGGCCGCCTGCGCTGGTACGCCGCTTCC
CTGGTGGGTGGCGCCGCCATCCTGCTCGGCGCGCTGCTGCTGGCCTAA
Translation:
MNLLPLTFLFPLVGFLLLSFSRGRWSENLSALVGVGSVGLSALSAAWAIFSFHSSPPEGGAYSLVLWQWMAA
GDFSSNFTLYLDGLSVTMLGVVTGVGFLIHLFASWYMRGETGYSRFFAYTNLFIASMLFLVLGDNLLFLYFG
WEGVGLCSYLLIGFYYSNRNNGNAALKAFIVTRVGDVFMAFGLFILFQQFGTLNIQELLVLAPQKFPEGNLW
LTLATLALLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARCHGLFTLAPDILELVGIV
GAVTLVLAGFAALVQTDIKRILAYSTMSQIGYMFLALGVGAWDAAIFHLMTHAFFKALLFLASGAVIIACHH
EQNIFKMGGLWKKLPLAYASFVVGGAALAALPFLTAGFYSKDEILWEAFASGHRELLIAGLVGAFLTSIYTF
RLIFVAFHGEPKTEAHAGHGISHWLPLSVLIVLSTFVGALITPPLAGVLPESVGHAGGEAKHSLELASGAIA
IAGILLAGLLFLGQRRFVSALAKSAPGRFFGTWWYHAWGFDWLYDKLFVKPYLLLCQLLGRDPIDRTLGVVP
FSVRGGHNLLSLTENGRLRWYAASLVGGAAILLGALLLA*
AnnotationID:463GeneID:454
AnnotatorUID: diggins
Modification Date/Time: 2005-04-29 11:11:49
Gene Name:nuoL
Confidence Code:2
GeneProduct:NADH dehydrogenase I chain L
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1009
Secondary Functional Category(ies):
EC Number:1.6.5.3
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69712.1|AE016607_6 (AE016789) NADH dehydrogenase I, L subunit [Pseudomonas putida
           KT2440]
          Length = 617

 Score = 1040 bits (2690), Expect = 0.0
 Identities = 510/615 (82%), Positives = 559/615 (90%)

>gb|AAO56853.1| (AE016868) NADH dehydrogenase I, L subunit [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 617

 Score = 1035 bits (2675), Expect = 0.0
 Identities = 504/614 (82%), Positives = 553/614 (90%)

>gb|AAN54084.1|AE015546_1 (AE015546) NADH dehydrogenase I, L subunit [Shewanella oneidensis
           MR-1]
          Length = 615

 Score =  895 bits (2312), Expect = 0.0
 Identities = 446/614 (72%), Positives = 515/614 (83%)






 
Structural Features:
COG1009, NuoL, NADH:ubiquinone oxidoreductase subunit 5 (chain L)/
Multisubunit Na+/H+ antiporter, MnhA subunit 
[Energy production and conversion / Inorganic ion transport and metabolism]
CD-Length = 606 residues,  99.8% aligned
Score =  445 bits (1146), Expect = 8e-126
Identities = 275/619 (44%), Positives = 356/619 (57%), Gaps = 26/619 (4%)








   
Genomic Context:






   
Comment:


















    ReferenceID:911




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18053




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33199




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:454_cdd.html


    		




 
URL:

















    ReferenceID:34582




  
Author/Investigator(s):
        Camacho Carvajal MM, Wijfjes AH, Mulders IH, Lugtenberg BJ, Bloemberg GV.





  
Title:
        Characterization of NADH dehydrogenases of Pseudomonas fluorescens WCS365 and their role in competitive root colonization.




  
PubMed:
        12118882


MedLine:
        




    
Source:
Mol Plant Microbe Interact. 2002 Jul;15(7):662-71.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12118882

















    ReferenceID:34592




  
Author/Investigator(s):
        Dupuis A, Peinnequin A, Chevallet M, Lunardi J, Darrouzet E, Pierrard B, Procaccio V, Issartel JP.





  
Title:
        Identification of five Rhodobacter capsulatus genes encoding the equivalent of ND subunits of the mitochondrial NADH-ubiquinone oxidoreductase.




  
PubMed:
        8566820


MedLine:
        




    
Source:
Gene. 1995 Dec 29;167(1-2):99-104.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8566820









           
Homologs By Global Alignment 

           

       



     
Gene ID:454
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
776
                           PA2647_tr
                           translation of PA2647
                           615
                           99.51
                           99.83
                           0
                           3164.0
     

     
     
33859
                           gb|AAG06035.1|AE004693_6
                           (AE004693) NADH dehydrogenase I chain L [Pseudomonas aeruginosa PAO1]
                           615
                           99.51
                           99.83
                           0
                           3164.0
     

     
     
33860
                           gb|AAN69712.1|AE016607_6
                           (AE016789) NADH dehydrogenase I, L subunit [Pseudomonas putida KT2440]
                           617
                           82.65
                           90.59
                           2
                           2690.0
     

     
     
33861
                           gb|AAL89574.1|
                           (AF281148) NADH dehydrogenase I subunit L [Pseudomonas fluorescens]
                           617
                           81.84
                           90.27
                           2
                           2685.0
     

     
     
33862
                           gb|AAO56853.1|
                           (AE016868) NADH dehydrogenase I, L subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           617
                           81.68
                           89.62
                           2
                           2675.0
     

     






      

          
GC ORFID: 43456How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2647Glimmer Score: 

          
Start: 2364874Stop: 2366721

          
Length: 1848


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA2647 translation of PA2647Homolog Bit Score 1223.0

          
Other Homologs:  psyr_15may02_Scaffold17_revised_gene2775 (ORF: BLASTX 1037.0), t_PA2647 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACCTTCTACCTCTTACCTTCCTGTTCCCCCTGGTCGGCTTCCTGCTGCTGTCGTTCTCCCGCGGCAGG 
TGGTCGGAAAACCTTTCGGCGCTGGTCGGCGTCGGCTCGGTCGGCCTCTCCGCCCTGAGCGCGGCCTGGGCC 
ATCTTCAGCTTCCACAGCAGTCCGCCGGAAGGCGGCGCCTACAGCCTGGTGCTGTGGCAGTGGATGGCGGCC 
GGCGACTTCAGCAGCAACTTCACCCTGTACCTGGACGGCCTCTCGGTAACCATGCTCGGCGTGGTTACCGGC 
GTCGGCTTCCTGATCCACCTGTTCGCCTCCTGGTACATGCGTGGCGAAACCGGCTATTCGCGCTTCTTCGCC 
TACACCAACCTGTTCATTGCCAGCATGCTGTTCCTGGTGCTGGGCGACAACCTGCTGTTCCTCTACTTCGGC 
TGGGAAGGCGTGGGCCTGTGCAGCTACCTGCTGATCGGCTTCTACTACAGCAATCGCAACAACGGTAACGCG 
GCGCTCAAGGCATTCATCGTCACCCGCGTCGGCGACGTGTTCATGGCTTTCGGCCTGTTCATCCTGTTCCAG 
CAGTTCGGCACCCTGAACATCCAGGAGCTGCTGGTGCTGGCGCCGCAGAAGTTCCCGGAAGGCAACCTCTGG 
CTGACCCTGGCGACCCTGGCCCTGCTCGGCGGCGCGGTCGGCAAGTCCGCCCAACTGCCGCTGCAGACCTGG 
CTGGCCGACGCCATGGCCGGCCCGACCCCGGTCTCCGCGCTGATCCACGCCGCCACCATGGTGACCGCCGGC 
GTCTACCTGATCGCCCGTTGCCACGGCCTGTTCACCCTGGCCCCGGACATCCTCGAACTGGTCGGCATCGTC 
GGCGCGGTGACCCTGGTCCTGGCCGGCTTCGCCGCCCTGGTGCAGACCGATATCAAGCGCATCCTCGCCTAC 
TCGACCATGAGCCAGATCGGCTACATGTTCCTCGCCCTCGGCGTCGGCGCCTGGGACGCGGCGATCTTCCAC 
CTGATGACCCACGCCTTCTTCAAGGCCCTGCTGTTCCTCGCCTCGGGCGCGGTGATCATCGCCTGCCACCAC 
GAGCAGAACATCTTCAAGATGGGCGGCTTGTGGAAGAAGCTGCCGCTGGCCTACGCCAGCTTCGTGGTCGGC 
GGTGCGGCGCTGGCCGCCCTGCCCTTCCTCACCGCGGGCTTCTACTCCAAGGACGAGATCCTCTGGGAGGCC 
TTCGCCAGCGGCCATCGCGAGTTGCTGATCGCCGGCCTGGTGGGTGCGTTCCTGACCTCGATCTACACCTTC 
CGCCTGATCTTCGTCGCCTTCCACGGCGAGCCGAAGACCGAAGCCCACGCCGGCCACGGGATTTCCCACTGG 
CTGCCGCTCAGCGTGCTGATCGTCCTGTCGACCTTCGTCGGCGCCCTGATCACCCCGCCGCTGGCCGGCGTC 
CTGCCGGAAAGCGTCGGCCACGCCGGTGGCGAAGCCAAGCACAGCCTGGAACTGGCCTCGGGCGCCATCGCC 
ATCGCCGGCATCCTGCTGGCCGGCCTGCTGTTCCTCGGCCAGCGCCGCTTCGTCAGCGCCCTGGCGAAGAGC 
GCCCCGGGTCGCTTCTTCGGTACCTGGTGGTACCACGCCTGGGGCTTCGACTGGCTGTACGACAAGCTGTTC 
GTGAAACCCTACCTGCTGCTCTGCCAACTGCTGGGGCGCGACCCCATCGACCGTACCCTGGGCGTCGTTCCG 
TTCAGCGTGCGCGGCGGACACAACCTCCTCAGCCTCACCGAGAACGGCCGCCTGCGCTGGTACGCCGCTTCC 
CTGGTGGGTGGCGCCGCCATCCTGCTCGGCGCGCTGCTGCTGGCCTAA