ViewGene
Locus Name:
GeneID:
Location:

GeneID:4524
TrivialName: PA14_57960
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_57960
PAO1 Ortholog Locus:PA4464
Sequence Length:465
Protein Length:154
Start: 5158354
Stop: 5158818
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07852.1|AE004860_8 (AE004860) nitrogen regulatory IIA protein [Pseudomonas aeruginosa PAO1]
Identities = 154/154 (100%), Positives = 154/154 (100%)
Sequence:
ATGATCCGACTTGAGCAAATCCTGACCCCCGGCCGTTCCCTCGTGAACGTGCCGGGCGGCAGCAAGAAACGC
GTACTCGAACAAATCGCCAACCTGGTAGCCAGGGAGCTGCCGGAGTTCGACGCCCAGACCATCTTCGAAAAC
CTGGTGGCCCGGGAAAAACTCGGCTCCACCGGCTTCGGCAATGGCATCGCCATTCCGCACTGCCGCCTGTCT
GGCTGCCAGAGCCCCATCAGCGCCGTGCTGCACCTGGATGCTCCCGTGGACTTCGACGCGCTGGACGGCGCG
CCGGTCGACCTGCTCTTCGTCTTGCTGGTACCGGAGGCCGCGACCGAAGAACATCTCGAACTGCTCCGGCAG
ATCGCCGCCATGCTCGACCGTGCCGACGTACGCGACCGCCTGCGCAGCGCTCCCACTGCCGAAGCGCTTTAT
CAGATCGTGGTAGACGTACAGAACGGGCAATGA
Translation:
MIRLEQILTPGRSLVNVPGGSKKRVLEQIANLVARELPEFDAQTIFENLVAREKLGSTGFGNGIAIPHCRLS
GCQSPISAVLHLDAPVDFDALDGAPVDLLFVLLVPEAATEEHLELLRQIAAMLDRADVRDRLRSAPTAEALY
QIVVDVQNGQ*
AnnotationID:4530GeneID:4524
AnnotatorUID: diggins
Modification Date/Time: 2005-05-03 07:07:30
Gene Name:ptsN
Confidence Code:2
GeneProduct:nitrogen regulatory IIA protein
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1762,cd00211
Secondary Functional Category(ies):
EC Number:2.7.1.69
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAD43114.1| (AJ496594) phosphotransferase enzyme IIA [Pseudomonas stutzeri]
          Length = 154

 Score =  250 bits (638), Expect = 2e-66
 Identities = 121/153 (79%), Positives = 141/153 (92%)


>gb|AAO57904.1| (AE016872) PTS IIA-like nitrogen-regulatory protein PtsN
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 154

 Score =  244 bits (624), Expect = 9e-65
 Identities = 119/153 (77%), Positives = 137/153 (89%)








 
Structural Features:
COG1762, PtsN, Phosphotransferase system mannitol /
fructose-specific IIA domain (Ntr-type) 
[Carbohydrate transport and metabolism / Signal transduction mechanisms]
CD-Length = 152 residues,  98.7% aligned
Score =  123 bits (311), Expect = 1e-29
Identities = 59/151 (39%), Positives = 89/151 (58%), Gaps = 3/151 (1%)

cd00211, PTS_IIA_fru, PTS_IIA, PTS system, fructose / mannitol specific IIA subunit.
CD-Length = 136 residues, 100.0% aligned
Score =  121 bits (304), Expect = 1e-28
Identities = 53/140 (37%), Positives = 81/140 (57%), Gaps = 4/140 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:9049




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:26191




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31504




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4524_cdd.html


    		




 
URL:

















    ReferenceID:32853




  
Author/Investigator(s):
        Rogers MJ, Ohgi T, Plumbridge J, Soll D.





  
Title:
        Nucleotide sequences of the Escherichia coli nagE and nagB genes: the structural genes for the N-acetylglucosamine transport protein of the bacterial phosphoenolpyruvate: sugar phosphotransferase system and for glucosamine-6-phosphate deaminase.




  
PubMed:
        3284790


MedLine:
        




    
Source:
Gene. 1988;62(2):197-207.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3284790

















    ReferenceID:32854




  
Author/Investigator(s):
        Jones-Mortimer MC, Kornberg HL.





  
Title:
        Amino-sugar transport systems of Escherichia coli K12




  
PubMed:
        6252281


MedLine:
        




    
Source:
J Gen Microbiol. 1980 Apr;117(2):369-76.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6252281

















    ReferenceID:32855




  
Author/Investigator(s):
        Cases I, Lopez JA, Albar JP, De Lorenzo V.





  
Title:
        Evidence of multiple regulatory functions for the PtsN (IIA(Ntr)) protein of Pseudomonas putida.




  
PubMed:
        11208802


MedLine:
        




    
Source:
J Bacteriol. 2001 Feb;183(3):1032-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11208802









           
Homologs By Global Alignment 

           

       



     
Gene ID:4524
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6879
                           PA4464_tr
                           translation of PA4464
                           154
                           99.99
                           99.99
                           0
                           768.0
     

     
     
183863
                           gb|AAG07852.1|AE004860_8
                           (AE004860) nitrogen regulatory IIA protein [Pseudomonas aeruginosa PAO1]
                           154
                           99.99
                           99.99
                           0
                           768.0
     

     
     
183864
                           gb|AAA19795.1|
                           (L26916) ORF2 [Pseudomonas aeruginosa]
                           154
                           99.35
                           99.35
                           0
                           762.0
     

     
     
183865
                           emb|CAD43114.1|
                           (AJ496594) phosphotransferase enzyme IIA [Pseudomonas stutzeri]
                           154
                           78.57
                           91.55
                           0
                           636.0
     

     
     
183866
                           gb|AAO57904.1|
                           (AE016872) PTS IIA-like nitrogen-regulatory protein PtsN [Pseudomonas syringae pv. tomato str. DC3000]
                           154
                           77.27
                           88.96
                           0
                           624.0
     

     






      

          
GC ORFID: 47526How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4464Glimmer Score: 

          
Start: 4422033Stop: 4422497

          
Length: 465


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4464 translation of PA4464Homolog Bit Score 300.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5549 (ORF: BLASTX 245.0), t_PA4464 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGATCCGACTTGAGCAAATCCTGACCCCCGGCCGTTCCCTCGTGAACGTGCCGGGCGGCAGCAAGAAACGC 
GTACTCGAACAAATCGCCAACCTGGTAGCCAGGGAGCTGCCGGAGTTCGACGCCCAGACCATCTTCGAAAAC 
CTGGTGGCCCGGGAAAAACTCGGCTCCACCGGCTTCGGCAATGGCATCGCCATTCCGCACTGCCGCCTGTCT 
GGCTGCCAGAGCCCCATCAGCGCCGTGCTGCACCTGGATGCTCCCGTGGACTTCGACGCGCTGGACGGCGCG 
CCGGTCGACCTGCTCTTCGTCTTGCTGGTACCGGAGGCCGCGACCGAAGAACATCTCGAACTGCTCCGGCAG 
ATCGCCGCCATGCTCGACCGTGCCGACGTACGCGACCGCCTGCGCAGCGCTCCCACTGCCGAAGCGCTTTAT 
CAGATCGTGGTAGACGTACAGAACGGGCAATGA