ViewGene
Locus Name:
GeneID:
Location:

GeneID:4520
TrivialName: PA14_70260
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_70260
PAO1 Ortholog Locus:PA5321
Sequence Length:456
Protein Length:151
Start: 6263598
Stop: 6264053
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08706.1|AE004944_10 (AE004944) deoxyuridine 5'-triphosphate nucleotidohydrolase [Pseudomonas aeruginosa PAO1]
Identities = 149/151 (98%), Positives = 150/151 (99%)
Sequence:
ATGCACTCTCTCCAAGCCAAGATCCTCGACCCCCGCCTGGGCTCCGACTTCCCGCTCCCGCAGTACGCCACG
CCGGGCTCGGCCGGCCTCGACCTGCGCGCCATGCTCAAGGAAGACACCGTCCTCGGCCCGGGCCAGACCCTG
CTGATTCCCACCGGCCTGTCCATCTACATCGCCGATCCCGGCCTCGCCGCCCTGGTTCTGCCGCGCTCCGGC
CTGGGCCACAAGCACGGCATCGTGCTGGGCAACCTGGTCGGCCTGATCGACTCGGACTACCAGGGCGAGCTG
ATGGTGTCCTGCTGGAACCGCGGCGAGTCGCCGTTCACCATCGCCGTCGGCGAGCGCATCGCGCAGTTGGTA
CTGGTGCCGGTGGTGCAGGCGCATTTCGAGCTGGTCGAGCAGTTCGACGAAAGCCAGCGCGGCGCCGGCGGC
TTCGGCCATTCCGGCAGCCACTGA
Translation:
MHSLQAKILDPRLGSDFPLPQYATPGSAGLDLRAMLKEDTVLGPGQTLLIPTGLSIYIADPGLAALVLPRSG
LGHKHGIVLGNLVGLIDSDYQGELMVSCWNRGESPFTIAVGERIAQLVLVPVVQAHFELVEQFDESQRGAGG
FGHSGSH*
AnnotationID:4526GeneID:4520
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 15:03:50
Gene Name:dut
Confidence Code:2
GeneProduct:deoxyuridine 5'-triphosphate nucleotidohydrolase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(18) Nucleotide biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00692
Secondary Functional Category(ies):
EC Number:3.6.1.23
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70851.1|AE016729_9 (AE016793) deoxyuridine 5-triphosphate nucleotidohydrolase
           [Pseudomonas putida KT2440]
          Length = 151

 Score =  285 bits (729), Expect = 6e-77
 Identities = 137/151 (90%), Positives = 146/151 (96%)

>gb|AAO53638.1| (AE016856) deoxyuridine 5'triphosphate nucleotidohydrolase
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 151

 Score =  277 bits (709), Expect = 1e-74
 Identities = 135/151 (89%), Positives = 144/151 (95%)

>gb|AAN57221.1|AE015858_2 (AE015858) deoxyuridine 5'-triphosphate nucleotidohydrolase
           [Shewanella oneidensis MR-1]
          Length = 152

 Score =  232 bits (591), Expect = 6e-61
 Identities = 104/147 (70%), Positives = 129/147 (87%)






 
Structural Features:
pfam00692, dUTPase, dUTPase. dUTPase hydrolyses dUTP to dUMP and pyrophosphate.
CD-Length = 129 residues,  98.4% aligned
Score =  125 bits (316), Expect = 2e-30
Identities = 64/133 (48%), Positives = 83/133 (62%), Gaps = 6/133 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:9041




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:26183




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34779




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4520_cdd.html


    		




 
URL:

















    ReferenceID:35702




  
Author/Investigator(s):
        Lundberg LG, Thoresson HO, Karlstrom OH, Nyman PO.





  
Title:
        Nucleotide sequence of the structural gene for dUTPase of Escherichia coli K-12.




  
PubMed:
        6139280


MedLine:
        




    
Source:
EMBO J. 1983;2(6):967-71.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6139280









           
Homologs By Global Alignment 

           

       



     
Gene ID:4520
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6875
                           PA5321_tr
                           translation of PA5321
                           151
                           98.67
                           99.33
                           0
                           771.0
     

     
     
183776
                           gb|AAG08706.1|AE004944_10
                           (AE004944) deoxyuridine 5'-triphosphate nucleotidohydrolase [Pseudomonas aeruginosa PAO1]
                           151
                           98.67
                           99.33
                           0
                           771.0
     

     
     
183777
                           gb|AAN70851.1|AE016729_9
                           (AE016793) deoxyuridine 5-triphosphate nucleotidohydrolase [Pseudomonas putida KT2440]
                           151
                           90.72
                           96.68
                           0
                           729.0
     

     
     
183778
                           gb|AAO53638.1|
                           (AE016856) deoxyuridine 5'triphosphate nucleotidohydrolase [Pseudomonas syringae pv. tomato str. DC3000]
                           151
                           89.40
                           95.36
                           0
                           709.0
     

     
     
183792
                           gb|AAO71269.1|
                           (AE016847) deoxyuridine 5'-triphosphate nucleotidohydrolase [Salmonella enterica subsp. enterica serovar Typhi Ty2]
                           151
                           71.52
                           80.13
                           0
                           563.0
     

     






      

          
GC ORFID: 47522How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5321Glimmer Score: 

          
Start: 5527287Stop: 5527742

          
Length: 456


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5321 translation of PA5321Homolog Bit Score 301.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3932 (ORF: BLASTX 278.0), t_PA5321 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCACTCTCTCCAAGCCAAGATCCTCGACCCCCGCCTGGGCTCCGACTTCCCGCTCCCGCAGTACGCCACG 
CCGGGCTCGGCCGGCCTCGACCTGCGCGCCATGCTCAAGGAAGACACCGTCCTCGGCCCGGGCCAGACCCTG 
CTGATTCCCACCGGCCTGTCCATCTACATCGCCGATCCCGGCCTCGCCGCCCTGGTTCTGCCGCGCTCCGGC 
CTGGGCCACAAGCACGGCATCGTGCTGGGCAACCTGGTCGGCCTGATCGACTCGGACTACCAGGGCGAGCTG 
ATGGTGTCCTGCTGGAACCGCGGCGAGTCGCCGTTCACCATCGCCGTCGGCGAGCGCATCGCGCAGTTGGTA 
CTGGTGCCGGTGGTGCAGGCGCATTTCGAGCTGGTCGAGCAGTTCGACGAAAGCCAGCGCGGCGCCGGCGGC 
TTCGGCCATTCCGGCAGCCACTGA