ViewGene
Locus Name:
GeneID:
Location:

GeneID:4517
TrivialName: PA14_62490
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_62490
PAO1 Ortholog Locus:PA4723
Sequence Length:447
Protein Length:148
Start: 5574317
Stop: 5574763
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08109.1|AE004886_1 (AE004886) suppressor protein DksA [Pseudomonas aeruginosa PAO1]
Identities = 148/148 (100%), Positives = 148/148 (100%)
Sequence:
ATGTCCACCAAAGCAAAACAACAGAGCAGCCAGCAGATGACCCGCGGCTTCGAACCCTATCAGGAAACCAAG
GGCGAGGAGTACATGAGCGAGCGCATGCGCGCGCACTTCACCGCGATCCTGAACAAATGGAAACAGGAGCTG
ATGGAAGAGGTCGACCGTACCGTGCATCACATGCAGGACGAAGCGGCCAACTTCCCCGATCCGGCCGACCGC
GCCAGCCAGGAAGAAGAATTCAGCCTGGAACTGCGCGCCCGCGACCGCGAGCGCAAGCTGATCAAGAAGATC
GACGAGACCCTGCAACTGATCGAAGACGAAGAGTACGGCTGGTGCGACTCCTGCGGCGTCGAGATTGGCATC
CGTCGCCTGGAGGCACGCCCCACCGCGACCCTCTGCATCGACTGCAAGACCCTCGCGGAAATCCGCGAGAAG
CAACTCGGCTCCTGA
Translation:
MSTKAKQQSSQQMTRGFEPYQETKGEEYMSERMRAHFTAILNKWKQELMEEVDRTVHHMQDEAANFPDPADR
ASQEEEFSLELRARDRERKLIKKIDETLQLIEDEEYGWCDSCGVEIGIRRLEARPTATLCIDCKTLAEIREK
QLGS*
AnnotationID:4523GeneID:4517
AnnotatorUID: diggins
Modification Date/Time: 2005-05-08 17:05:56
Gene Name:dksA
Confidence Code:1
GeneProduct:suppressor protein DksA
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(25) Transcriptional regulators
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1734,pfam01258
Secondary Functional Category(ies):signal transduction mechanism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO54503.1| (AE016859) dnaK suppressor protein [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 146

 Score =  247 bits (630), Expect = 2e-65
 Identities = 121/147 (82%), Positives = 132/147 (89%), Gaps = 2/147 (1%)

>gb|AAN70266.1|AE016666_10 (AE016791) C4-type zinc finger protein, DksA/TraR family
           [Pseudomonas putida KT2440]
          Length = 148

 Score =  219 bits (558), Expect = 4e-57
 Identities = 107/147 (72%), Positives = 123/147 (83%), Gaps = 1/147 (0%)

>gb|AAN78672.1|AE016755_172 (AE016755) DnaK suppressor protein [Escherichia coli CFT073]
          Length = 157

 Score =  211 bits (537), Expect = 1e-54
 Identities = 100/131 (76%), Positives = 114/131 (87%)








 
Structural Features:
COG1734, DksA, DnaK suppressor protein [Signal transduction mechanisms]
CD-Length = 120 residues, 100.0% aligned
Score =  119 bits (299), Expect = 1e-28
Identities = 66/120 (55%), Positives = 84/120 (70%)

pfam01258, zf-dskA_traR, Prokaryotic dksA/traR C4-type zinc finger.
CD-Length = 70 residues, 100.0% aligned
Score = 60.5 bits (146), Expect = 7e-11
Identities = 30/71 (42%), Positives = 43/71 (60%), Gaps = 1/71 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:9035




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:26177




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37625




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4517_cdd.html


    		




 
URL:

















    ReferenceID:37744




  
Author/Investigator(s):
        Jude F, Kohler T, Branny P, Perron K, Mayer MP, Comte R, van Delden C.





  
Title:
        Posttranscriptional control of quorum-sensing-dependent virulence genes by DksA in Pseudomonas aeruginosa.




  
PubMed:
        12775693


MedLine:
        




    
Source:
J Bacteriol. 2003 Jun;185(12):3558-66.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12775693

















    ReferenceID:37745




  
Author/Investigator(s):
        Branny P, Pearson JP, Pesci EC, Kohler T, Iglewski BH, Van Delden C.





  
Title:
        Inhibition of quorum sensing by a Pseudomonas aeruginosa dksA homologue.




  
PubMed:
        11160083


MedLine:
        




    
Source:
J Bacteriol. 2001 Mar;183(5):1531-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11160083









           
Homologs By Global Alignment 

           

       



     
Gene ID:4517
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6872
                           PA4723_tr
                           translation of PA4723
                           148
                           99.99
                           99.99
                           0
                           771.0
     

     
     
183714
                           gb|AAG08109.1|AE004886_1
                           (AE004886) suppressor protein DksA [Pseudomonas aeruginosa PAO1]
                           148
                           99.99
                           99.99
                           0
                           771.0
     

     
     
183715
                           gb|AAF85762.1|AF062653_1
                           (AF062653) multicopy mutation suppressor DksA [Pseudomonas aeruginosa]
                           148
                           99.99
                           99.99
                           0
                           771.0
     

     
     
183716
                           gb|AAO54503.1|
                           (AE016859) dnaK suppressor protein [Pseudomonas syringae pv. tomato str. DC3000]
                           148
                           81.75
                           89.18
                           2
                           632.0
     

     
     
183717
                           gb|AAN70266.1|AE016666_10
                           (AE016791) C4-type zinc finger protein, DksA/TraR family [Pseudomonas putida KT2440]
                           149
                           71.81
                           82.55
                           2
                           560.0
     

     






      

          
GC ORFID: 47519How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4723Glimmer Score: 

          
Start: 4837997Stop: 4838443

          
Length: 447


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4723 translation of PA4723Homolog Bit Score 301.0

          
Other Homologs:  psyr_15may02_Scaffold15_revised_gene2512 (ORF: BLASTX 246.0), t_PA4723 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCCACCAAAGCAAAACAACAGAGCAGCCAGCAGATGACCCGCGGCTTCGAACCCTATCAGGAAACCAAG 
GGCGAGGAGTACATGAGCGAGCGCATGCGCGCGCACTTCACCGCGATCCTGAACAAATGGAAACAGGAGCTG 
ATGGAAGAGGTCGACCGTACCGTGCATCACATGCAGGACGAAGCGGCCAACTTCCCCGATCCGGCCGACCGC 
GCCAGCCAGGAAGAAGAATTCAGCCTGGAACTGCGCGCCCGCGACCGCGAGCGCAAGCTGATCAAGAAGATC 
GACGAGACCCTGCAACTGATCGAAGACGAAGAGTACGGCTGGTGCGACTCCTGCGGCGTCGAGATTGGCATC 
CGTCGCCTGGAGGCACGCCCCACCGCGACCCTCTGCATCGACTGCAAGACCCTCGCGGAAATCCGCGAGAAG 
CAACTCGGCTCCTGA