ViewGene
Locus Name:
GeneID:
Location:

GeneID:4508
TrivialName: PA14_21310
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_21310
PAO1 Ortholog Locus:PA3302
Sequence Length:471
Protein Length:156
Start: 1846907
Stop: 1846437
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06690.1|AE004752_6 (AE004752) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 156/156 (100%), Positives = 156/156 (100%)
Sequence:
ATGAGCCAGGTCCAGAACATTCCCTACGCCGAGCTAGAGGTCGGGCAAAAGGCCGAGTACACCAGCAGCATC
GCCGAGCGCGACCTGCAGTTGTTCGCCGCAGTCTCCGGCGACCGCAACCCGGTGCACCTGGACGCCGCCTAC
GCGGCCACCACCCAGTTCAAGGAACGCATCGCCCACGGCATGCTCAGCGGCGCCCTGATCAGCGCGGCGATC
GCCACCGTGCTGCCCGGCCCAGGCACCATCTATCTCGGCCAGACCCTGCGCTTCACCCGTCCGGTCAAGCTC
GGCGACGACCTGAAGGTCGAACTGGAAGTCCTCGAGAAGCTGCCGAAGAACCGCGTGCGCATGGCCACGCGG
GTGTTCAACCAGGCAGGCAAGCAGGTGGTCGACGGCGAAGCGGAGATCATGGCGCCGGAAGAGAAGCTCAGC
GTCGAGCTGGCCGAACTGCCGCCGATCAGCATCGGCTGA
Translation:
MSQVQNIPYAELEVGQKAEYTSSIAERDLQLFAAVSGDRNPVHLDAAYAATTQFKERIAHGMLSGALISAAI
ATVLPGPGTIYLGQTLRFTRPVKLGDDLKVELEVLEKLPKNRVRMATRVFNQAGKQVVDGEAEIMAPEEKLS
VELAELPPISIG*
AnnotationID:4514GeneID:4508
AnnotatorUID: diggins
Modification Date/Time: 2005-09-11 16:04:49
Gene Name:phaJ1
Confidence Code:3
GeneProduct:(R)-specific enoyl-CoA hydratase
Cell Localization:()
Synonyms:maoC
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:putative acyl dehydratase
Functional Category Confidence Code:5
COGs:pfam01575,COG2030
Secondary Functional Category(ies):chaperones & heat shock proteins
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57556.1| (AE016870) MaoC-like domain protein [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 173

 Score =  214 bits (544), Expect = 2e-55
 Identities = 107/156 (68%), Positives = 125/156 (80%)

>gb|AAM97601.1|AF299293_1 (AF299293) (R)-specific enoyl-CoA hydratase PhaJ1 [Pseudomonas
           oleovorans]
          Length = 156

 Score =  203 bits (517), Expect = 2e-52
 Identities = 101/151 (66%), Positives = 120/151 (79%)

>gb|AAN70125.1|AE016652_6 (AE016791) enoyl-CoA hydratase, R-specific [Pseudomonas putida
           KT2440]
          Length = 156

 Score =  202 bits (515), Expect = 4e-52
 Identities = 101/151 (66%), Positives = 120/151 (79%)






 
Structural Features:
pfam01575, MaoC_dehydratas, MaoC like domain.
CD-Length = 123 residues,  92.7% aligned
Score = 96.9 bits (241), Expect = 1e-21
Identities = 38/114 (33%), Positives = 57/114 (50%), Gaps = 2/114 (1%)

COG2030, MaoC, Acyl dehydratase [Lipid metabolism]
CD-Length = 159 residues,  90.6% aligned
Score =  113 bits (284), Expect = 8e-27
Identities = 52/144 (36%), Positives = 75/144 (52%), Gaps = 8/144 (5%)







   
Genomic Context:






   
Comment:


















    ReferenceID:9017




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:26159




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32250




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4508_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:4508
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6863
                           PA3302_tr
                           translation of PA3302
                           156
                           99.99
                           99.99
                           0
                           771.0
     

     
     
183384
                           gb|AAG06690.1|AE004752_6
                           (AE004752) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
                           156
                           99.99
                           99.99
                           0
                           771.0
     

     
     
183385
                           dbj|BAA92740.1|
                           (AB040025) (R)-specific enoyl-CoA hydratase [Pseudomonas aeruginosa]
                           156
                           99.99
                           99.99
                           0
                           771.0
     

     
     
183389
                           gb|AAG16296.1|
                           (AY007576) (R)-specific enoyl-CoA hydratase [Pseudomonas putida]
                           157
                           64.96
                           77.70
                           2
                           513.0
     

     
     
183388
                           gb|AAN70125.1|AE016652_6
                           (AE016791) enoyl-CoA hydratase, R-specific [Pseudomonas putida KT2440]
                           157
                           64.96
                           77.70
                           2
                           513.0
     

     






      

          
GC ORFID: 47510How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3302Glimmer Score: 

          
Start: 3107024Stop: 3107494

          
Length: 471


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3302 translation of PA3302Homolog Bit Score 301.0

          
Other Homologs:  psyr_15may02_Scaffold5_revised_gene6066 (ORF: BLASTX 212.0), t_PA3302 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCCAGGTCCAGAACATTCCCTACGCCGAGCTAGAGGTCGGGCAAAAGGCCGAGTACACCAGCAGCATC 
GCCGAGCGCGACCTGCAGTTGTTCGCCGCAGTCTCCGGCGACCGCAACCCGGTGCACCTGGACGCCGCCTAC 
GCGGCCACCACCCAGTTCAAGGAACGCATCGCCCACGGCATGCTCAGCGGCGCCCTGATCAGCGCGGCGATC 
GCCACCGTGCTGCCCGGCCCAGGCACCATCTATCTCGGCCAGACCCTGCGCTTCACCCGTCCGGTCAAGCTC 
GGCGACGACCTGAAGGTCGAACTGGAAGTCCTCGAGAAGCTGCCGAAGAACCGCGTGCGCATGGCCACGCGG 
GTGTTCAACCAGGCAGGCAAGCAGGTGGTCGACGGCGAAGCGGAGATCATGGCGCCGGAAGAGAAGCTCAGC 
GTCGAGCTGGCCGAACTGCCGCCGATCAGCATCGGCTGA