ViewGene
Locus Name:
GeneID:
Location:

GeneID:45
TrivialName: PA14_18970
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_18970
PAO1 Ortholog Locus:PA3487
Sequence Length:3312
Protein Length:1103
Start: 1638973
Stop: 1635662
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06875.1|AE004769_8 (AE004769) phospholipase D [Pseudomonas aeruginosa PAO1]
Identities = 1098/1103 (99%), Positives = 1099/1103 (99%), Gaps = 4/1103 (0%)
Sequence:
ATGTTGCAGAAGAAGCCCTACAACGGCCTCCACGAAAAGGAACTCAACCAGATCAACCAGCAGGATGGCAGC
CCCTGCGTAGCCATTTCCGCACCGGGCTGTTTCATCAAGGGGTCCAACCTCTTTTCCGAAAAGCGGGCCGGT
AACCGGGTCCGCTTCTTCACCACCGGCCGGGACTACTTCAGCGACCTGGCCAGCGCCCTCGACAGCGCCCTC
GACAGCGCCAGTTCGAGCATCTTCATCACCGGCTGGCAGGTCAACTACGACGTCCTCCTCGACGGCCGCCGC
AGCCTCTGGCAGTGCCTGCGCCAGGCGCTGGAGCGCTCGCCCGCGCTGAAGGTCTACGTGATGCCCTGGCTC
AGCCCTTCCGGCAGCCTCGGCACCTATGACTTCGAAACCATGCTCGCGGTGTTCCAGCTCAACGCCGGACTC
GAGGGCGGCGCGCGCGCCTTCTGTACGCCGGCGATCCAGCAGAGCGACATGCAGGGACTGGGCGTCGCCTTC
TCCCACCACCAGAAATCCGTGGTGATCGACAACCGCATCGGCTATGTCGGCGGGATCGACCTTGCCTATGGC
CGTCGCGACGACAACGATTTCAGCCTCGACGCCTCCGGGCGCCGGGGCAACGACGCCTATAATCCCGGCCTA
CCGCATCTTGGCTGGATGGCGGAGGACGAACACGTCAGCTCCATGGGCCTGATGATGGCCACCCTGTTCGAC
CTTTCCAGGCCGCTCGCCAGCCTGACGCTGCATGCGCCCACCCTTCGCCTGTCGCCCTTCCCGCACATCGCC
GCCAGCGACGAACCGCTGCTGAGCATTCCCCTCGCACCCAGCCGCGCCCGCGCCCTCAACGGCGCCGCCTAC
CTTTCCGACCTGTTCCGCAGCCCGATGCTGCCCTCCCTGCAATGGCTCGGCCGCGCCTACAACAGCTCGAAA
GAAGGCCTGGACGAAGGCTTCGAACGGCTCGATGCGCTCAGGCGGCAGATGGTCGCCAGCAGCATCCGCGCC
ATTGCCAACCTGATCGCCGACAACCTCGACGCCCTGCCCATCGAGCCTGAACTCGAACGCCGCCTGCGCGCC
TGGCTGGAGGAACTGCGCACGGCCGCGCTCAACCTGCCGGAAGCCCTGCGGATCAAGAGCCTGCTCCTGATC
AACCAGTGGATGAGCGAAACCGAACTGGGCCAGGTCCTGACCCTGATCAGCGGCAAGGGCTTCGAAGATATC
CCGCAGAACCTGAGCGGCAAGGCCGGCGAGTTGGCCGGCTCGCTGTTCTGGACACTCCACAGGCTGTTGCAG
GCCCGCGCCGGAGGCCACCAGCAACCCTATCGCTACCTGGACGAAGCGCCTCAGCCATTGGCCTCGCCGGAC
AACGCCCGGCTGGCCGCCGACCAGCCGCGCATGCCCTGGCAGGATGTGCATTGCCGCATCGAAGGGCCGTCG
GTGTACGACCTGGCGCGCAACTTCATCGACCGCTGGAACGGCCAGCAGGCCTACCTGGCCAAGACGCCGGCG
CTACAGGATACGGCGCTGGTACGCAGCGCCCTGGAAGCCGTGATGAAGTGGCTGAACAGCCTGGCCGCCGCC
GCGGGATTGGAGAACTACCTGGACGAGAAGCGCAACCTCCGGCTCGAACTGGACCCACCCACGCCCTGCTGG
ATCAACGCGCCGGAACAGTTGCCGCAAGAGCCGGAGGTGCGCCGCGGCGGCATGACCGTACAGGTACTGCGC
AGCGCCGCGGCGCGCATGCTCGAACAGGAGCAGGCCGGCCGCCTCGGCGCGGGAGTGAACCTGCCCTTGCAG
GTCGGCGTCTCCACCGAAGGAGTCCAGAGCAACTGCAAGGACGCCATGCTCCTGGCGATTTCCGGGGCGCAG
CAGTTCATCTATATCGAGAACCAGTTCTTCCAGAGCGAGTTCGGCAAGGAAGGCGAAGTTTTCAAGGATCTG
CCCCTTTCAGGCCCCATGGCCAGTCTGCGCGACGTTGGCAGCCTGCGACGCGACTTCGTCGTCCGGATACGC
TTGGAGGAAGCCCTGGAGCAGCGCGACCTCTGGCTGCTGGACTGGGCCGAAGTGGAGAAGATCGCCCAGGAG
CCTGGCACCGAGGCCCGCCAGTTCCTCAAGTCGATGCTCGCCATGTGGGGCGTGAACGCCCAGGGCTGGCTC
ACCCACAAGCTCGGCGAGGCCCAGCACGGCCTGCTCAACGAGATCGGCGAAGCCCTGGCGCGGCGCATCGAA
CGGGCCATCCAGCGCGAGCACCCGTTCCACGTCTACCTGGTCCTGCCGGTGCACCCGGAGGGCGCGCTGAAC
GTGCCGAACATCATGCACCAGGTGCACCTGACCCAGCAGAGCCTGGTGTTCGGCGAACAGAGCCTGGTCAAG
CGCATTCAGCGGCAGATGGCGCTCAAGGCCCTGGAAGGCAAGTCCGACCCGGCCCAGGCCCGGGAAATCATC
GAGCGCAAGGACGCTCGCGGTCGCCCGGTCTACGAACAGCAGGACTGGAGCCGCTACCTGACCCTGCTCAAC
CTGCGCACCTGGGCGGTGCTCGGCGGGCGGGTGGTCACCGAACAGATCTACGTGCACAGCAAGCTGCTGATC
GCCGACGACCGGGTGGCCATCCTCGGCAGCGCCAATATCAACGACCGCAGCCTGCAAGGCGAGCGCGACTCC
GAGCTGGCGGTGATGGTGCGCGACAGCGAGCCGCTGACGGTCAGGCTGGACGGCAAGAACGACGCCATCGTC
GGCAAGGCCATCCACCAGTTGCGGGTGAATTTGTGGAAGAAGCATTTTGGCTTGAGCCAGGGGCCAGGCGGT
TTCGTCAAGCCGGCCAGCGAGTTGAGTGCTTATCTGTCCATCCCGGCGGCTCAAGAGGCCTGGGAAGCTATA
CAAACATTAGCCAAGGAGAATACTCGTGCCTATGAACGGACTTTCAATTTTATTCCCCAAAATATCAGCCAG
ACTCAACTGCAACTGACTCCAGAGCCGCCTAAAGGATTTGAGGACGGATTTCCCGCCTCCATATGGCCAACC
TGGGCATACAGAAAACCTGGCGAGTTAAGGGCGGGCGGACAACTGATGGAGCCGATGCCATATCAGGAAATC
TTCTGGCGCTCATCTAATCTCACTTCGGTGAAAACCTTCCCACCACCCAACGGGGTTTCCGGTTTCATCACG
GCTCTACCAACCAGTTGGACCCGTGGTGAGCGCAATGATTCTGGCTTGAATCTAAGCATCCTGGCACATCAG
GACAGCAGATCGCTTCCCACCCAGGTCGCCATGAATGGAGATAGCTCGGCACAAGGAAAGCATCGCACATGA
Translation:
MLQKKPYNGLHEKELNQINQQDGSPCVAISAPGCFIKGSNLFSEKRAGNRVRFFTTGRDYFSDLASALDSAL
DSASSSIFITGWQVNYDVLLDGRRSLWQCLRQALERSPALKVYVMPWLSPSGSLGTYDFETMLAVFQLNAGL
EGGARAFCTPAIQQSDMQGLGVAFSHHQKSVVIDNRIGYVGGIDLAYGRRDDNDFSLDASGRRGNDAYNPGL
PHLGWMAEDEHVSSMGLMMATLFDLSRPLASLTLHAPTLRLSPFPHIAASDEPLLSIPLAPSRARALNGAAY
LSDLFRSPMLPSLQWLGRAYNSSKEGLDEGFERLDALRRQMVASSIRAIANLIADNLDALPIEPELERRLRA
WLEELRTAALNLPEALRIKSLLLINQWMSETELGQVLTLISGKGFEDIPQNLSGKAGELAGSLFWTLHRLLQ
ARAGGHQQPYRYLDEAPQPLASPDNARLAADQPRMPWQDVHCRIEGPSVYDLARNFIDRWNGQQAYLAKTPA
LQDTALVRSALEAVMKWLNSLAAAAGLENYLDEKRNLRLELDPPTPCWINAPEQLPQEPEVRRGGMTVQVLR
SAAARMLEQEQAGRLGAGVNLPLQVGVSTEGVQSNCKDAMLLAISGAQQFIYIENQFFQSEFGKEGEVFKDL
PLSGPMASLRDVGSLRRDFVVRIRLEEALEQRDLWLLDWAEVEKIAQEPGTEARQFLKSMLAMWGVNAQGWL
THKLGEAQHGLLNEIGEALARRIERAIQREHPFHVYLVLPVHPEGALNVPNIMHQVHLTQQSLVFGEQSLVK
RIQRQMALKALEGKSDPAQAREIIERKDARGRPVYEQQDWSRYLTLLNLRTWAVLGGRVVTEQIYVHSKLLI
ADDRVAILGSANINDRSLQGERDSELAVMVRDSEPLTVRLDGKNDAIVGKAIHQLRVNLWKKHFGLSQGPGG
FVKPASELSAYLSIPAAQEAWEAIQTLAKENTRAYERTFNFIPQNISQTQLQLTPEPPKGFEDGFPASIWPT
WAYRKPGELRAGGQLMEPMPYQEIFWRSSNLTSVKTFPPPNGVSGFITALPTSWTRGERNDSGLNLSILAHQ
DSRSLPTQVAMNGDSSAQGKHRT*
AnnotationID:55GeneID:45
AnnotatorUID: diggins
Modification Date/Time: 2005-04-19 12:12:01
Gene Name:pldA
Confidence Code:1
GeneProduct:phospholipase D
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:cd00138,smart00155
Secondary Functional Category(ies):
EC Number:3.1.1.4
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAC48494.1| (AL603642) putative phospholipase protein [Sinorhizobium meliloti]
          Length = 590

 Score = 54.3 bits (129), Expect = 4e-06
 Identities = 46/155 (29%), Positives = 68/155 (43%), Gaps = 18/155 (11%)








 
Structural Features:
cd00138, PLDc, Phospholipase D. Active site motifs
CD-Length = 176 residues,  72.2% aligned
Score = 55.1 bits (132), Expect = 5e-08
Identities = 30/142 (21%), Positives = 49/142 (34%), Gaps = 16/142 (11%)

pfam00614, PLDc, Phospholipase D
CD-Length = 28 residues,  92.9% aligned
Score = 41.8 bits (99), Expect = 4e-04
Identities = 10/26 (38%), Positives = 15/26 (57%)






   
Genomic Context:






   
Comment:


















    ReferenceID:93




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17235




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32056




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:45_cdd.html


    		




 
URL:

















    ReferenceID:34105




  
Author/Investigator(s):
        Wilderman PJ, Vasil AI, Johnson Z, Vasil ML.





  
Title:
        Genetic and biochemical analyses of a eukaryotic-like phospholipase D of Pseudomonas aeruginosa suggest horizontal acquisition and a role for persistence in a chronic pulmonary infection model.




  
PubMed:
        11136451


MedLine:
        




    
Source:
Mol Microbiol. 2001 Jan;39(2):291-303.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11136451









           
Homologs By Global Alignment 

           

       



     
Gene ID:45
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
86
                           PA3487_tr
                           translation of PA3487
                           1103
                           99.54
                           99.63
                           4
                           5685.0
     

     
     
11666
                           gb|AAG06875.1|AE004769_8
                           (AE004769) phospholipase D [Pseudomonas aeruginosa PAO1]
                           1103
                           99.54
                           99.63
                           4
                           5685.0
     

     






      

          
GC ORFID: 43047How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3487Glimmer Score: 

          
Start: 3314961Stop: 3318272

          
Length: 3312


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3487 translation of PA3487Homolog Bit Score 2193.0

          
Other Homologs:  t_PA3487 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTTGCAGAAGAAGCCCTACAACGGCCTCCACGAAAAGGAACTCAACCAGATCAACCAGCAGGATGGCAGC 
CCCTGCGTAGCCATTTCCGCACCGGGCTGTTTCATCAAGGGGTCCAACCTCTTTTCCGAAAAGCGGGCCGGT 
AACCGGGTCCGCTTCTTCACCACCGGCCGGGACTACTTCAGCGACCTGGCCAGCGCCCTCGACAGCGCCCTC 
GACAGCGCCAGTTCGAGCATCTTCATCACCGGCTGGCAGGTCAACTACGACGTCCTCCTCGACGGCCGCCGC 
AGCCTCTGGCAGTGCCTGCGCCAGGCGCTGGAGCGCTCGCCCGCGCTGAAGGTCTACGTGATGCCCTGGCTC 
AGCCCTTCCGGCAGCCTCGGCACCTATGACTTCGAAACCATGCTCGCGGTGTTCCAGCTCAACGCCGGACTC 
GAGGGCGGCGCGCGCGCCTTCTGTACGCCGGCGATCCAGCAGAGCGACATGCAGGGACTGGGCGTCGCCTTC 
TCCCACCACCAGAAATCCGTGGTGATCGACAACCGCATCGGCTATGTCGGCGGGATCGACCTTGCCTATGGC 
CGTCGCGACGACAACGATTTCAGCCTCGACGCCTCCGGGCGCCGGGGCAACGACGCCTATAATCCCGGCCTA 
CCGCATCTTGGCTGGATGGCGGAGGACGAACACGTCAGCTCCATGGGCCTGATGATGGCCACCCTGTTCGAC 
CTTTCCAGGCCGCTCGCCAGCCTGACGCTGCATGCGCCCACCCTTCGCCTGTCGCCCTTCCCGCACATCGCC 
GCCAGCGACGAACCGCTGCTGAGCATTCCCCTCGCACCCAGCCGCGCCCGCGCCCTCAACGGCGCCGCCTAC 
CTTTCCGACCTGTTCCGCAGCCCGATGCTGCCCTCCCTGCAATGGCTCGGCCGCGCCTACAACAGCTCGAAA 
GAAGGCCTGGACGAAGGCTTCGAACGGCTCGATGCGCTCAGGCGGCAGATGGTCGCCAGCAGCATCCGCGCC 
ATTGCCAACCTGATCGCCGACAACCTCGACGCCCTGCCCATCGAGCCTGAACTCGAACGCCGCCTGCGCGCC 
TGGCTGGAGGAACTGCGCACGGCCGCGCTCAACCTGCCGGAAGCCCTGCGGATCAAGAGCCTGCTCCTGATC 
AACCAGTGGATGAGCGAAACCGAACTGGGCCAGGTCCTGACCCTGATCAGCGGCAAGGGCTTCGAAGATATC 
CCGCAGAACCTGAGCGGCAAGGCCGGCGAGTTGGCCGGCTCGCTGTTCTGGACACTCCACAGGCTGTTGCAG 
GCCCGCGCCGGAGGCCACCAGCAACCCTATCGCTACCTGGACGAAGCGCCTCAGCCATTGGCCTCGCCGGAC 
AACGCCCGGCTGGCCGCCGACCAGCCGCGCATGCCCTGGCAGGATGTGCATTGCCGCATCGAAGGGCCGTCG 
GTGTACGACCTGGCGCGCAACTTCATCGACCGCTGGAACGGCCAGCAGGCCTACCTGGCCAAGACGCCGGCG 
CTACAGGATACGGCGCTGGTACGCAGCGCCCTGGAAGCCGTGATGAAGTGGCTGAACAGCCTGGCCGCCGCC 
GCGGGATTGGAGAACTACCTGGACGAGAAGCGCAACCTCCGGCTCGAACTGGACCCACCCACGCCCTGCTGG 
ATCAACGCGCCGGAACAGTTGCCGCAAGAGCCGGAGGTGCGCCGCGGCGGCATGACCGTACAGGTACTGCGC 
AGCGCCGCGGCGCGCATGCTCGAACAGGAGCAGGCCGGCCGCCTCGGCGCGGGAGTGAACCTGCCCTTGCAG 
GTCGGCGTCTCCACCGAAGGAGTCCAGAGCAACTGCAAGGACGCCATGCTCCTGGCGATTTCCGGGGCGCAG 
CAGTTCATCTATATCGAGAACCAGTTCTTCCAGAGCGAGTTCGGCAAGGAAGGCGAAGTTTTCAAGGATCTG 
CCCCTTTCAGGCCCCATGGCCAGTCTGCGCGACGTTGGCAGCCTGCGACGCGACTTCGTCGTCCGGATACGC 
TTGGAGGAAGCCCTGGAGCAGCGCGACCTCTGGCTGCTGGACTGGGCCGAAGTGGAGAAGATCGCCCAGGAG 
CCTGGCACCGAGGCCCGCCAGTTCCTCAAGTCGATGCTCGCCATGTGGGGCGTGAACGCCCAGGGCTGGCTC 
ACCCACAAGCTCGGCGAGGCCCAGCACGGCCTGCTCAACGAGATCGGCGAAGCCCTGGCGCGGCGCATCGAA 
CGGGCCATCCAGCGCGAGCACCCGTTCCACGTCTACCTGGTCCTGCCGGTGCACCCGGAGGGCGCGCTGAAC 
GTGCCGAACATCATGCACCAGGTGCACCTGACCCAGCAGAGCCTGGTGTTCGGCGAACAGAGCCTGGTCAAG 
CGCATTCAGCGGCAGATGGCGCTCAAGGCCCTGGAAGGCAAGTCCGACCCGGCCCAGGCCCGGGAAATCATC 
GAGCGCAAGGACGCTCGCGGTCGCCCGGTCTACGAACAGCAGGACTGGAGCCGCTACCTGACCCTGCTCAAC 
CTGCGCACCTGGGCGGTGCTCGGCGGGCGGGTGGTCACCGAACAGATCTACGTGCACAGCAAGCTGCTGATC 
GCCGACGACCGGGTGGCCATCCTCGGCAGCGCCAATATCAACGACCGCAGCCTGCAAGGCGAGCGCGACTCC 
GAGCTGGCGGTGATGGTGCGCGACAGCGAGCCGCTGACGGTCAGGCTGGACGGCAAGAACGACGCCATCGTC 
GGCAAGGCCATCCACCAGTTGCGGGTGAATTTGTGGAAGAAGCATTTTGGCTTGAGCCAGGGGCCAGGCGGT 
TTCGTCAAGCCGGCCAGCGAGTTGAGTGCTTATCTGTCCATCCCGGCGGCTCAAGAGGCCTGGGAAGCTATA 
CAAACATTAGCCAAGGAGAATACTCGTGCCTATGAACGGACTTTCAATTTTATTCCCCAAAATATCAGCCAG 
ACTCAACTGCAACTGACTCCAGAGCCGCCTAAAGGATTTGAGGACGGATTTCCCGCCTCCATATGGCCAACC 
TGGGCATACAGAAAACCTGGCGAGTTAAGGGCGGGCGGACAACTGATGGAGCCGATGCCATATCAGGAAATC 
TTCTGGCGCTCATCTAATCTCACTTCGGTGAAAACCTTCCCACCACCCAACGGGGTTTCCGGTTTCATCACG 
GCTCTACCAACCAGTTGGACCCGTGGTGAGCGCAATGATTCTGGCTTGAATCTAAGCATCCTGGCACATCAG 
GACAGCAGATCGCTTCCCACCCAGGTCGCCATGAATGGAGATAGCTCGGCACAAGGAAAGCATCGCACATGA