ViewGene
Locus Name:
GeneID:
Location:

GeneID:4498
TrivialName: PA14_24020
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_24020
PAO1 Ortholog Locus:PA3101
Sequence Length:447
Protein Length:148
Start: 2087598
Stop: 2088044
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06489.1|AE004734_7 (AE004734) general secretion pathway protein G [Pseudomonas aeruginosa PAO1]
Identities = 147/148 (99%), Positives = 148/148 (100%)
Sequence:
GTGAATCAGAGCCGCCCATTGCAGCGTCGCCAACAGTCGGGCTTCACCCTGATCGAAATCATGGTCGTGGTG
GTCATCCTCGGCATCCTCGCCGCCCTGGTGGTGCCGCAGGTGATGAGCCGTCCCGACCAGGCCAAGGTCACC
GTGGCCAAGGGCGACATCAAGGCCATCGCTGCCGCGCTGGACATGTACAAGCTGGACAACTTCGCCTATCCG
AGCACCCAGCAGGGCCTCGAGGCGCTGGTGAAGAAGCCCACCGGCAACCCGCAGCCGAAGAACTGGAACAAG
GACGGCTACCTGAAGAAGCTGCCGGTCGATCCGTGGGGCAATCCCTACCAGTACCTGGCGCCGGGCACCAAG
GGCCCGTTCGACCTGTATTCGCTGGGCGCCGACGGCAAGGAAGGCGGCAGCGACAACGACGCCGAAATCGGC
AACTGGGACAACTGA
Translation:
MNQSRPLQRRQQSGFTLIEIMVVVVILGILAALVVPQVMSRPDQAKVTVAKGDIKAIAAALDMYKLDNFAYP
STQQGLEALVKKPTGNPQPKNWNKDGYLKKLPVDPWGNPYQYLAPGTKGPFDLYSLGADGKEGGSDNDAEIG
NWDN*
AnnotationID:4504GeneID:4498
AnnotatorUID: diggins
Modification Date/Time: 2005-04-13 06:06:06
Gene Name:xcpT
Confidence Code:1
GeneProduct: general secretion pathway protein G
Cell Localization:()
Synonyms:pddA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(19) Protein secretion/export apparatus
Alternate Gene Product Name: PilD-dependent protein PddA
Functional Category Confidence Code:5
COGs:COG2165
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAD16815.1| (AL646073) PUTATIVE GENERAL SECRETION PATHWAY G TRANSMEMBRANE
           PROTEIN [Ralstonia solanacearum]
          Length = 156

 Score =  199 bits (506), Expect = 4e-51
 Identities = 93/146 (63%), Positives = 114/146 (78%)








 
Structural Features:
COG2165, PulG, Type II secretory pathway, pseudopilin PulG 
[Cell motility and secretion / Intracellular trafficking and secretion]
CD-Length = 149 residues, 100.0% aligned
Score = 97.4 bits (241), Expect = 6e-22
Identities = 65/149 (43%), Positives = 87/149 (58%), Gaps = 8/149 (5%)






   
Genomic Context:






   
Comment:


















    ReferenceID:15102




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:26139




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32423




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4498_cdd.html


    		




 
URL:

















    ReferenceID:33461




  
Author/Investigator(s):
        Durand E, Bernadac A, Ball G, Lazdunski A, Sturgis JN, Filloux A.





  
Title:
        Type II protein secretion in Pseudomonas aeruginosa: the pseudopilus is a multifibrillar and adhesive structure.




  
PubMed:
        12700254


MedLine:
        




    
Source:
J Bacteriol. 2003 May;185(9):2749-58.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12700254

















    ReferenceID:33462




  
Author/Investigator(s):
        Koster M, Bitter W, Tommassen J.





  
Title:
        Protein secretion mechanisms in Gram-negative bacteria.




  
PubMed:
        11111906


MedLine:
        




    
Source:
Int J Med Microbiol. 2000 Oct;290(4-5):325-31.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11111906

















    ReferenceID:33463




  
Author/Investigator(s):
        Kagami Y, Ratliff M, Surber M, Martinez A, Nunn DN.





  
Title:
        Type II protein secretion by Pseudomonas aeruginosa: genetic suppression of a conditional mutation in the pilin-like component XcpT by the cytoplasmic component XcpR.




  
PubMed:
        9466269


MedLine:
        




    
Source:
Mol Microbiol. 1998 Jan;27(1):221-33.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9466269

















    ReferenceID:33464




  
Author/Investigator(s):
        Lu HM, Motley ST, Lory S.





  
Title:
        Interactions of the components of the general secretion pathway: role of Pseudomonas aeruginosa type IV pilin subunits in complex formation and extracellular protein secretion.




  
PubMed:
        9282737


MedLine:
        




    
Source:
Mol Microbiol. 1997 Jul;25(2):247-59.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9282737









           
Homologs By Global Alignment 

           

       



     
Gene ID:4498
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6846
                           PA3101_tr
                           translation of PA3101
                           148
                           99.32
                           99.99
                           0
                           776.0
     

     
     
183083
                           gb|AAG06489.1|AE004734_7
                           (AE004734) general secretion pathway protein G [Pseudomonas aeruginosa PAO1]
                           148
                           98.64
                           99.99
                           0
                           773.0
     

     
     
183084
                           emb|CAA44535.1|
                           (X62666) secretion protein XcpT [Pseudomonas aeruginosa]
                           148
                           94.59
                           95.94
                           6
                           743.0
     

     
     
183085
                           gb|AAA25946.1|
                           (M80792) PilD-dependent protein [Pseudomonas aeruginosa]
                           148
                           93.91
                           95.94
                           6
                           740.0
     

     
     
183086
                           gb|AAC83358.1|
                           (AF092918) outer membrane secretion protein T [Pseudomonas alcaligenes]
                           148
                           75.00
                           80.40
                           8
                           580.0
     

     






      

          
GC ORFID: 47500How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3101Glimmer Score: 

          
Start: 2866333Stop: 2865887

          
Length: 447


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3101 translation of PA3101Homolog Bit Score 303.0

          
Other Homologs:  t_PA0681 (ORF: BLASTX 160.0), t_PA3101 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGAATCAGAGCCGCCCATTGCAGCGTCGCCAACAGTCGGGCTTCACCCTGATCGAAATCATGGTCGTGGTG 
GTCATCCTCGGCATCCTCGCCGCCCTGGTGGTGCCGCAGGTGATGAGCCGTCCCGACCAGGCCAAGGTCACC 
GTGGCCAAGGGCGACATCAAGGCCATCGCTGCCGCGCTGGACATGTACAAGCTGGACAACTTCGCCTATCCG 
AGCACCCAGCAGGGCCTCGAGGCGCTGGTGAAGAAGCCCACCGGCAACCCGCAGCCGAAGAACTGGAACAAG 
GACGGCTACCTGAAGAAGCTGCCGGTCGATCCGTGGGGCAATCCCTACCAGTACCTGGCGCCGGGCACCAAG 
GGCCCGTTCGACCTGTATTCGCTGGGCGCCGACGGCAAGGAAGGCGGCAGCGACAACGACGCCGAAATCGGC 
AACTGGGACAACTGA