ViewGene

Locus Name:
GeneID:
Location:

GeneID:4482
TrivialName: PA14_45790
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_45790
PAO1 Ortholog Locus:PA1444
Sequence Length:474
Protein Length:157
Start: 4070678
Stop: 4070205
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04833.1|AE004574_4 (AE004574) flagellar motor switch protein FliN [Pseudomonas aeruginosa PAO1]
Identities = 157/157 (100%), Positives = 157/157 (100%)
Sequence:
ATGGCAGACGAAGAAAAAGTGACCACCGAGGAACAGGCACTGGCCGACGAATGGGCGGCGGCGCTGGCCGAG
GCGGGCGACGCCAGCCAGGACGACATCGATGCGCTGATGGCCCAGGGCGGCGCGACCCCCGTGGCGGAGCCG
AGCACCCCGCGGGCGCCGATGGAAGAGTTCGGCGCTTCGCCGAAGGCGCCGACCATTTCCGGCCTGGAGGGG
CCCAACCTGGATGTGATCCTGGACATCCCGGTGACCATCTCCATGGAAGTCGGGCATACCGACATCAGCATC
CGCAACCTGCTGCAGCTGAACCAGGGGTCGGTGATCGAACTCGATCGCCTGGCCGGCGAGCCGCTGGACGTA
CTGGTCAACGGCACCCTGATCGCCCACGGCGAGGTGGTGGTGGTGAACGAGAAGTTCGGCATCCGCCTGACC
GACGTGATCAGTCCCAGCGAACGCATCAAGAAGCTGCGCTGA
Translation:
MADEEKVTTEEQALADEWAAALAEAGDASQDDIDALMAQGGATPVAEPSTPRAPMEEFGASPKAPTISGLEG
PNLDVILDIPVTISMEVGHTDISIRNLLQLNQGSVIELDRLAGEPLDVLVNGTLIAHGEVVVVNEKFGIRLT
DVISPSERIKKLR*
AnnotationID:4488GeneID:4482
AnnotatorUID: diggins
Modification Date/Time: 2005-02-24 09:09:10
Gene Name:fliN
Confidence Code:1
GeneProduct:flagellar motor switch protein FliN
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(16) Motility & Attachment
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1886,pfam01052
Secondary Functional Category(ies):chemotaxis
EC Number:
Status:ACTIVE
Pathway:
Homology:
>gb|AAN69935.1|AE016632_6 (AE016790) flagellar motor switch protein FliN [Pseudomonas putida
           KT2440]
          Length = 161

 Score =  218 bits (554), Expect = 1e-56
 Identities = 121/158 (76%), Positives = 131/158 (82%), Gaps = 4/158 (2%)

>gb|AAO55488.1| (AE016862) flagellar motor switch protein FliN [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 152

 Score =  216 bits (550), Expect = 4e-56
 Identities = 122/158 (77%), Positives = 131/158 (82%), Gaps = 7/158 (4%)

>gb|AAF95270.1| (AE004285) flagellar motor switch protein FliN [Vibrio cholerae]
          Length = 138

 Score =  166 bits (420), Expect = 4e-41
 Identities = 93/149 (62%), Positives = 111/149 (74%), Gaps = 16/149 (10%)

Structural Features:
COG1886, FliN, Flagellar motor switch/type III secretory pathway protein 
[Cell motility and secretion / Intracellular trafficking and secretion]
CD-Length = 136 residues, 100.0% aligned
Score =  102 bits (255), Expect = 2e-23
Identities = 50/145 (34%), Positives = 80/145 (55%), Gaps = 9/145 (6%)

pfam01052, SpoA, Surface presentation of antigens (SPOA) protein.
CD-Length = 77 residues, 100.0% aligned
Score = 85.6 bits (212), Expect = 3e-18
Identities = 37/77 (48%), Positives = 57/77 (74%)
Genomic Context:
Comment:
ReferenceID:8965
Author/Investigator(s): GenNotator
Title:
PubMed:
MedLine:
Source:
Reference Type:BLASTP
Data:
URL:
ReferenceID:26107
Author/Investigator(s): GenNotator
Title: CDD Search
PubMed:
MedLine:
Source:
Reference Type:RPSBLAST
Data:
URL:
ReferenceID:37386
Author/Investigator(s):
Title: CDD Search
PubMed:
MedLine:
Source:
Reference Type:Misc (text/plain)
Data:4488_cdd.html
URL:
ReferenceID:37984
Author/Investigator(s): Simpson DA, Ramphal R, Lory S.
Title: Characterization of Pseudomonas aeruginosa fliO, a gene involved in flagellar biosynthesis and adherence.
PubMed: 7622217
MedLine:
Source:
Infect Immun. 1995 Aug;63(8):2950-7.
Reference Type:Journal
Data:
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7622217
ReferenceID:37985
Author/Investigator(s): Malakooti J, Komeda Y, Matsumura P.
Title: DNA sequence analysis, gene product identification, and localization of flagellar motor components of Escherichia coli.
PubMed: 2651416
MedLine:
Source:
J Bacteriol. 1989 May;171(5):2728-34.
Reference Type:Journal
Data:
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2651416
Homologs By Global Alignment
Gene ID:4482

Identity:

0% 10% 20% 30% 40% 50% 60% 70% 80% 90% 100%
HomologID Accession Description Length PctIdentity PctSimilarity Gaps Score
6826 PA1444_tr translation of PA1444 157 99.99 99.99 0 780.0
182647 gb|AAG04833.1|AE004574_4 (AE004574) flagellar motor switch protein FliN [Pseudomonas aeruginosa PAO1] 157 99.99 99.99 0 780.0
182648 gb|AAA79754.1| (L39832) flagellar protein [Pseudomonas aeruginosa] 157 99.36 99.36 0 772.0
182651 gb|AAO55488.1| (AE016862) flagellar motor switch protein FliN [Pseudomonas syringae pv. tomato str. DC3000] 158 77.84 83.54 7 558.0
182650 gb|AAN69935.1|AE016632_6 (AE016790) flagellar motor switch protein FliN [Pseudomonas putida KT2440] 165 74.54 80.00 12 563.0
GC ORFID: 47484How Found: BLASTX
GC_TrimmedSeqID: 69Blast Result ID
Subject Sequence Name: t_PA1444Glimmer Score:
Start: 883231Stop: 883704
Length: 474
Start Codon: ATGTruncated Start:
Stop Codon: TGATruncated Stop:
Homolog: t_PA1444 translation of PA1444Homolog Bit Score 305.0
Other Homologs: psyr_15may02_Scaffold8_revised_gene7929 (ORF: BLASTX 217.0), t_PA1444 (ORF: GLIMMER)
GC ORF Sequence
ATGGCAGACGAAGAAAAAGTGACCACCGAGGAACAGGCACTGGCCGACGAATGGGCGGCGGCGCTGGCCGAG
GCGGGCGACGCCAGCCAGGACGACATCGATGCGCTGATGGCCCAGGGCGGCGCGACCCCCGTGGCGGAGCCG
AGCACCCCGCGGGCGCCGATGGAAGAGTTCGGCGCTTCGCCGAAGGCGCCGACCATTTCCGGCCTGGAGGGG
CCCAACCTGGATGTGATCCTGGACATCCCGGTGACCATCTCCATGGAAGTCGGGCATACCGACATCAGCATC
CGCAACCTGCTGCAGCTGAACCAGGGGTCGGTGATCGAACTCGATCGCCTGGCCGGCGAGCCGCTGGACGTA
CTGGTCAACGGCACCCTGATCGCCCACGGCGAGGTGGTGGTGGTGAACGAGAAGTTCGGCATCCGCCTGACC
GACGTGATCAGTCCCAGCGAACGCATCAAGAAGCTGCGCTGA