ViewGene
Locus Name:
GeneID:
Location:

GeneID:4476
TrivialName: PA14_62150
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_62150
PAO1 Ortholog Locus:PA4695
Sequence Length:492
Protein Length:163
Start: 5545937
Stop: 5545446
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08081.1|AE004883_5 (AE004883) acetolactate synthase isozyme III small subunit [Pseudomonas aeruginosa PAO1]
Identities = 163/163 (100%), Positives = 163/163 (100%)
Sequence:
ATGCGACACATCATTTCCCTGCTGCTGGAAAACGAGCCAGGCGCATTGTCCCGCGTGGTCGGCCTGTTCTCC
CAACGCAACTACAACATCGAAAGCCTGACCGTGGCGCCGACCGAGGACCCGACCCTGTCGCGTCTGACGCTG
ACCACCGTGGGGCACGATGAGGTGATCGAGCAGATCACCAAGAACCTCAACAAGCTGATCGAAGTGGTCAAG
CTGGTGGACCTGTCGGAAAGCGCCCATATCGAGCGCGAGCTGATGCTGGTGAAGGTCAAGGCCACGGGCGCC
CAGCGCGCCGAGGTCAAGCGCACCACCGATATCTTCCGTGGGCAGATCGTCGACGTCACCAGTAGCGTCTAT
ACCGTGCAACTGGCGGGTACCAGCGACAAACTGGACAGCTTCATCCAGGCCATCGGCACTGCGTCGATCCTG
GAAGTGGTCCGCAGTGGCGTCACTGGCATCGCCCGTGGCGACAAGACTCTGAGCATCTGA
Translation:
MRHIISLLLENEPGALSRVVGLFSQRNYNIESLTVAPTEDPTLSRLTLTTVGHDEVIEQITKNLNKLIEVVK
LVDLSESAHIERELMLVKVKATGAQRAEVKRTTDIFRGQIVDVTSSVYTVQLAGTSDKLDSFIQAIGTASIL
EVVRSGVTGIARGDKTLSI*
AnnotationID:4482GeneID:4476
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 20:08:48
Gene Name:ilvH
Confidence Code:2
GeneProduct:acetolactate synthase isozyme III small subunit
Cell Localization:()
Synonyms:ilvN
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:acetolactate synthase, small subunit
Functional Category Confidence Code:5
COGs:COG0440
Secondary Functional Category(ies):amino acid biosynthesis and metabolism
EC Number:4.1.3.18
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70252.1|AE016665_5 (AE016791) acetolactate synthase, small subunit [Pseudomonas putida
           KT2440]
          Length = 163

 Score =  300 bits (768), Expect = 2e-81
 Identities = 157/163 (96%), Positives = 161/163 (98%)

>gb|AAO54516.1| (AE016859) acetolactate synthase, small subunit [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 163

 Score =  299 bits (766), Expect = 4e-81
 Identities = 157/163 (96%), Positives = 161/163 (98%)

>emb|CAE35860.1| (BX640448) acetolactate synthase small subunit [Bordetella
           bronchiseptica]
          Length = 163

 Score =  224 bits (571), Expect = 2e-58
 Identities = 108/163 (66%), Positives = 139/163 (85%)






 
Structural Features:
COG0440, IlvH, Acetolactate synthase, small (regulatory) subunit 
[Amino acid transport and metabolism]
CD-Length = 163 residues,  98.8% aligned
Score =  187 bits (477), Expect = 5e-49
Identities = 85/161 (52%), Positives = 120/161 (74%)






   
Genomic Context:






   
Comment:


















    ReferenceID:8953




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:26095




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37606




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4476_cdd.html


    		




 
URL:

















    ReferenceID:37752




  
Author/Investigator(s):
        Squires CH, De Felice M, Devereux J, Calvo JM.





  
Title:
        Molecular structure of ilvIH and its evolutionary relationship to ilvG in Escherichia coli K12.




  
PubMed:
        6308579


MedLine:
        




    
Source:
Nucleic Acids Res. 1983 Aug 11;11(15):5299-313.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=6308579

















    ReferenceID:37755




  
Author/Investigator(s):
        Thao ML, Baumann P.





  
Title:
        Sequence analysis of a DNA fragment from buchnera aphidicola (Aphid endosymbiont) containing the genes dapD-htrA-ilvI-ilvH-ftsL-ftsI-murE




  
PubMed:
        0009688822


MedLine:
        




    
Source:
Curr Microbiol. 1998 Sep;37(3):214-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9688822









           
Homologs By Global Alignment 

           

       



     
Gene ID:4476
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6821
                           PA4695_tr
                           translation of PA4695
                           163
                           99.99
                           99.99
                           0
                           785.0
     

     
     
182436
                           gb|AAG08081.1|AE004883_5
                           (AE004883) acetolactate synthase isozyme III small subunit [Pseudomonas aeruginosa PAO1]
                           163
                           99.99
                           99.99
                           0
                           785.0
     

     
     
182437
                           gb|AAN70252.1|AE016665_5
                           (AE016791) acetolactate synthase, small subunit [Pseudomonas putida KT2440]
                           163
                           96.31
                           98.77
                           0
                           768.0
     

     
     
182438
                           gb|AAO54516.1|
                           (AE016859) acetolactate synthase, small subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           163
                           96.31
                           98.77
                           0
                           766.0
     

     
     
182445
                           dbj|BAC58616.1|
                           (AP005074) acetolactate synthase III, small subunit [Vibrio parahaemolyticus]
                           164
                           70.12
                           83.53
                           1
                           560.0
     

     






      

          
GC ORFID: 47478How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4695Glimmer Score: 

          
Start: 4809617Stop: 4809126

          
Length: 492


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4695 translation of PA4695Homolog Bit Score 306.0

          
Other Homologs:  psyr_15may02_Scaffold19_revised_gene2874 (ORF: BLASTX 299.0), t_PA4695 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGACACATCATTTCCCTGCTGCTGGAAAACGAGCCAGGCGCATTGTCCCGCGTGGTCGGCCTGTTCTCC 
CAACGCAACTACAACATCGAAAGCCTGACCGTGGCGCCGACCGAGGACCCGACCCTGTCGCGTCTGACGCTG 
ACCACCGTGGGGCACGATGAGGTGATCGAGCAGATCACCAAGAACCTCAACAAGCTGATCGAAGTGGTCAAG 
CTGGTGGACCTGTCGGAAAGCGCCCATATCGAGCGCGAGCTGATGCTGGTGAAGGTCAAGGCCACGGGCGCC 
CAGCGCGCCGAGGTCAAGCGCACCACCGATATCTTCCGTGGGCAGATCGTCGACGTCACCAGTAGCGTCTAT 
ACCGTGCAACTGGCGGGTACCAGCGACAAACTGGACAGCTTCATCCAGGCCATCGGCACTGCGTCGATCCTG 
GAAGTGGTCCGCAGTGGCGTCACTGGCATCGCCCGTGGCGACAAGACTCTGAGCATCTGA