ViewGene
Locus Name:
GeneID:
Location:

GeneID:4463
TrivialName: PA14_48460
AnnotatorUID: nikki
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_48460
PAO1 Ortholog Locus:
Sequence Length:1113
Protein Length:370
Start: 4309009
Stop: 4307897
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAN67808.1|AE016412_1 (AE016782) periplasmic polyamine-binding protein, putative [Pseudomonas putida KT2440]
Identities = 164/360 (45%), Positives = 222/360 (61%), Gaps = 4/360 (1%) Coding sequence may overlap with coding sequence of GeneID 5639.
Sequence:
ATGAAACGTTCCGCGCGACCGCTGCTCGCCGCGCTGCTCCTGGGCCTGGGCGTGGCCCAGGCGGAAGAGAAA
GTGCTGCGCCTGTATAACTGGGCCGACTACTTCGCGCCCGATACCCTCGCCGCGTTCACCCGGGAAACCGGC
ATCCGCGTGGTCTATGACGTGATGGACAGCAGCGAGACCCTCGAGGCCAAGTTGATGTCGGGGAACAGCGGC
TACGACCTTATCTTTCCCGGCGATACCGTGGCCGAGCGGCTGATGCGCGCCGGCGCCCTGCAGAAGCTCGAC
CCCCGGCGCCTGGAGCATCTCGACGAGATCGAGCCGGGCCTGCGCGAACTGCAAGCCCGCTACGCGCGTTCG
CGCGACGCCGCGGTGCCCTACACCTGGGGCACCATCGGCCTGACCTTCAACCGCGAGCAGATCGAACGGCGC
CTGGCCGACGCTCCGCTGGACAGCCTCGACCTGCTGTTCAAGCCGGAATTGGCGGCGAGATTCGCCGACTGC
GGGATTTCCCTGATCGACTCGCCGGACGAAGTGCTGGCGCTGGCCCTGCATTACCTGGGGCGCGATCCGCGC
AGCGGCAAGCCGCAGGACCTGGCCGCCGCGCTGGCCTTGCTGCAGCGCATCAAGCCCTACATTCGCAAGTTC
CAGTCGCAGCCGGTGGCGCAACTGGTGAACGAGGAGATCTGCCTGTCGCTGGGCTACAGCGGCGATGCGATC
CAGGCCCAGCGCGCCGCCGACGAAGCCGGCAAGGCACTGCGCTTCGTCTACCGGGTGCCGCGCGAAGGCACG
TCTCTGTGGATGGACAGCATGGCGATTCCGGTCGACGCGCAGCACCCGGAATATGCCTATGCGTTGATCAAC
TTCATCATGCGCCCGCAGAACATGGCCGCGATCAGCAGCGCTACCGGCTACCCGACCTCCAGCGCCGGGGCG
CGGGCCCTGGTATCCGCCGACATGCGCGACAACCCGGACATCTATCCGGACGCGGCGACCTACGCCCGGCTG
TTTCCCGGCCAGGACATCCCGCAGCGCGACATGCGGGCGCGGATGCGCGCCTGGACTACCTTCAAGACCACC
CGTTCCGATCCCGTTCCCGAGGACCCGAAATGA
Translation:
MKRSARPLLAALLLGLGVAQAEEKVLRLYNWADYFAPDTLAAFTRETGIRVVYDVMDSSETLEAKLMSGNSG
YDLIFPGDTVAERLMRAGALQKLDPRRLEHLDEIEPGLRELQARYARSRDAAVPYTWGTIGLTFNREQIERR
LADAPLDSLDLLFKPELAARFADCGISLIDSPDEVLALALHYLGRDPRSGKPQDLAAALALLQRIKPYIRKF
QSQPVAQLVNEEICLSLGYSGDAIQAQRAADEAGKALRFVYRVPREGTSLWMDSMAIPVDAQHPEYAYALIN
FIMRPQNMAAISSATGYPTSSAGARALVSADMRDNPDIYPDAATYARLFPGQDIPQRDMRARMRAWTTFKTT
RSDPVPEDPK*
AnnotationID:4469GeneID:4463
AnnotatorUID: danlee
Modification Date/Time: 2005-10-08 16:04:29
Gene Name:
Confidence Code:3
GeneProduct:probable polyamine binding/transport protein
Cell Localization:(8) Periplasmic
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0687
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
gi|48729338|ref|ZP_00263089.1|  COG0687: Spermidine/putrescine-binding periplasmic protein 
[Pseudomonas fluorescens PfO-1]
     Length=367
     Score =  518 bits (1333),  Expect = 2e-145
     Identities = 255/363 (70%), Positives = 298/363 (82%), Gaps = 4/363 (1%)

gi|24983731|gb|AAN67808.1|  periplasmic polyamine-binding protein, putative [Pseudomonas putida KT2440]
     Length=362
     Score =  311 bits (798),  Expect = 2e-83
     Identities = 164/360 (45%), Positives = 222/360 (61%), Gaps = 4/360 (1%)

gi|11350806|pir||E83607  polyamine transport protein PA0300 [imported] - Pseudomonas aeruginosa (strain PAO1)
     Length=367
     Score =  307 bits (787),  Expect = 3e-82
     Identities = 157/364 (43%), Positives = 227/364 (62%), Gaps = 5/364 (1%)






 
Structural Features:
COG0687, PotD, Spermidine/putrescine-binding periplasmic protein [Amino acid transport and metabolism].
             CD-Length = 363 residues,  92.3% aligned
             Score =  256 bits (654), Expect = 4e-69






   
Genomic Context:






   
Comment:


















    ReferenceID:8927




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:26069




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:4463
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
182197
                           gb|AAN67808.1|AE016412_1
                           (AE016782) periplasmic polyamine-binding protein, putative [Pseudomonas putida KT2440]
                           385
                           43.63
                           59.48
                           38
                           812.0
     

     
     
182200
                           gb|AAN69316.1|AE016568_11
                           (AE016788) periplasmic polyamine-binding protein, putative [Pseudomonas putida KT2440]
                           388
                           42.52
                           58.76
                           39
                           784.0
     

     
     
182199
                           gb|AAO58733.1|
                           (AE016875) putrescine ABC transporter, periplasmic putrescine-binding protein [Pseudomonas syringae pv. tomato str. DC3000]
                           374
                           41.97
                           61.76
                           13
                           780.0
     

     
     
6804
                           PA0300_tr
                           translation of PA0300
                           376
                           41.75
                           60.63
                           15
                           795.0
     

     
     
182198
                           gb|AAG03689.1|AE004468_1
                           (AE004468) polyamine transport protein [Pseudomonas aeruginosa PAO1]
                           376
                           41.75
                           60.63
                           15
                           795.0
     

     






      

          
GC ORFID: 47465How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0300Glimmer Score: 

          
Start: 644899Stop: 646011

          
Length: 1113


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0300 translation of PA0300Homolog Bit Score 307.0

          
Other Homologs:  t_PA0301 (ORF: BLASTX 300.0), psyr_15may02_Scaffold6_revised_gene6511 (ORF: BLASTX 300.0), t_PA1410 (ORF: BLASTX 293.0), t_PA2711 (ORF: BLASTX 284.0), t_PA2592 (ORF: BLASTX 267.0), t_PA0300 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAACGTTCCGCGCGACCGCTGCTCGCCGCGCTGCTCCTGGGCCTGGGCGTGGCCCAGGCGGAAGAGAAA 
GTGCTGCGCCTGTATAACTGGGCCGACTACTTCGCGCCCGATACCCTCGCCGCGTTCACCCGGGAAACCGGC 
ATCCGCGTGGTCTATGACGTGATGGACAGCAGCGAGACCCTCGAGGCCAAGTTGATGTCGGGGAACAGCGGC 
TACGACCTTATCTTTCCCGGCGATACCGTGGCCGAGCGGCTGATGCGCGCCGGCGCCCTGCAGAAGCTCGAC 
CCCCGGCGCCTGGAGCATCTCGACGAGATCGAGCCGGGCCTGCGCGAACTGCAAGCCCGCTACGCGCGTTCG 
CGCGACGCCGCGGTGCCCTACACCTGGGGCACCATCGGCCTGACCTTCAACCGCGAGCAGATCGAACGGCGC 
CTGGCCGACGCTCCGCTGGACAGCCTCGACCTGCTGTTCAAGCCGGAATTGGCGGCGAGATTCGCCGACTGC 
GGGATTTCCCTGATCGACTCGCCGGACGAAGTGCTGGCGCTGGCCCTGCATTACCTGGGGCGCGATCCGCGC 
AGCGGCAAGCCGCAGGACCTGGCCGCCGCGCTGGCCTTGCTGCAGCGCATCAAGCCCTACATTCGCAAGTTC 
CAGTCGCAGCCGGTGGCGCAACTGGTGAACGAGGAGATCTGCCTGTCGCTGGGCTACAGCGGCGATGCGATC 
CAGGCCCAGCGCGCCGCCGACGAAGCCGGCAAGGCACTGCGCTTCGTCTACCGGGTGCCGCGCGAAGGCACG 
TCTCTGTGGATGGACAGCATGGCGATTCCGGTCGACGCGCAGCACCCGGAATATGCCTATGCGTTGATCAAC 
TTCATCATGCGCCCGCAGAACATGGCCGCGATCAGCAGCGCTACCGGCTACCCGACCTCCAGCGCCGGGGCG 
CGGGCCCTGGTATCCGCCGACATGCGCGACAACCCGGACATCTATCCGGACGCGGCGACCTACGCCCGGCTG 
TTTCCCGGCCAGGACATCCCGCAGCGCGACATGCGGGCGCGGATGCGCGCCTGGACTACCTTCAAGACCACC 
CGTTCCGATCCCGTTCCCGAGGACCCGAAATGA