ViewGene
Locus Name:
GeneID:
Location:

GeneID:446
TrivialName: PA14_62970
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_62970
PAO1 Ortholog Locus:PA4761
Sequence Length:1914
Protein Length:637
Start: 5621040
Stop: 5619127
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08147.1|AE004889_10 (AE004889) DnaK protein [Pseudomonas aeruginosa PAO1]
Identities = 637/637 (100%), Positives = 637/637 (100%)
Sequence:
ATGGGCAAAATCATTGGCATCGACCTGGGTACCACCAACTCCTGCGTGGCTATCCTGGAGAACGGTAACGTC
AAGGTCATCGAGAACGCCGAGGGCGCGCGTACCACCCCCTCGATCATCGCCTACACCAACGATGGCGAAACC
CTGGTGGGCCAGCCGGCCAAGCGCCAGGCGGTCACCAACCCGCAGAACACCCTGTATGCGGTGAAGCGCCTG
ATCGGCCGTCGCTTCGAAGAGAACGTGGTACAGAAAGACATCCAGATGGTGCCGTACAGCATCGTCAAGGCC
GACAACGGCGACGCCTGGGTGGAAGTGAAGGGCCAGAAGATGGCGCCTCCGCAGATTTCCGCCGAAGTGCTG
AAGAAAATGAAGAAGACCGCCGAAGACTACCTCGGCGAGCCGGTCACCGAAGCGGTGATCACCGTTCCGGCC
TACTTCAACGACAGCCAGCGCCAGGCCACCAAGGACGCCGGCCGCATCGCCGGCCTCGACGTCAAGCGGATC
ATCAACGAGCCGACCGCGGCGGCGCTGGCCTACGGCCTGGACAAGGCCAAGGGCGACCACACCGTGATCGTC
TATGACCTGGGCGGCGGTACCTTCGACGTGTCGGTGATCGAGATCGCCGAAGTCGACGGTGAGCACCAGTTC
GAAGTGCTGGCCACCAACGGCGACACCTTCCTCGGCGGCGAAGACTTCGACATCCGCCTGATCGACTACCTC
GTCGACGAGTTCAAGAAGGAAAGCGGCATCAACCTGAAGGGCGACCCGCTGGCCATGCAGCGTCTGAAGGAA
GCGGCCGAGAAAGCCAAGATCGAGCTGTCCTCGACCCAGCAGACCGACGTCAACCTGCCGTACGTGACTGCC
GACGCGAGCGGTCCGAAGCACCTGAACGTCAAGGTTTCCCGCGCCAAGCTGGAGTCCCTGGTCGAAGACCTG
GTGCAGCGCACCATCGAGCCGTGCCGCACCGCGCTGAAGGATGCCGGCCTGGATGTTTCCGATATCCATGAA
GTGATCCTGGTCGGCGGCCAGACCCGCATGCCGCTGGTACAGAAGACCGTTGCCGAGTTCTTCGGCAAGGAA
GCGCGCAAGGACGTCAACCCGGACGAAGCCGTGGCTGTCGGTGCCGCCATCCAGGGCGCGGTCCTGGCCGGC
GACGTGAAGGACGTGCTGCTGCTCGACGTGACCCCGCTGACCCTCGGCATCGAGACCTTGGGCGGCGTGATG
ACCGGTCTGATCGAGAAGAACACCACCATCCCGACCAAGAAGTCGCAGGTGTTCTCCACCGCCGACGACAAC
CAGGGCGCGGTGACCATCCACGTGCTGCAGGGCGAGCGCAAGCAGGCCGCGCAGAACAAGTCGCTGGGCAAG
TTCGACCTGGCCGACATTCCGCCGGCTCCGCGCGGCGTGCCGCAGATCGAGGTGACCTTCGATATCGATGCC
AACGGCATCCTGCACGTCTCGGCGAAGGACAAGGCCACTGGCAAGCAGCAGTCCATCGTGATCAAGGCATCC
TCCGGCCTGTCCGAGGATGAGATCCAGCAGATGGTCCGCGATGCCGAGGCGAACGCCGAGGAGGACCGCAAG
TTCGAGGAACTGGCTGCCGCTCGCAACCAGGGCGACGCGCTGGTCCACGCGACCCGCAAGATGATCACCGAG
GCGGGCGACAAGGCCACCGCCGAGGACAAGGCGACCATCGAGAAGGCGCTGGGCGAGCTGGAAGCGGCGGTG
AAGGGCGACGACAAGGCCGAGATCGAGGCCAAGATGAACGCTCTGTCCCAGGCTTCCACCCCGCTGGCGCAG
AAGATGTACGCCGAACAGGCCCAGCAGGGCGAAGACGCTCCCCAGGGCGAGCAGGCGAAAGCCGCTGACGAC
GTGGTGGACGCCGAGTTCGAAGAGGTCAAGGACAACAAGTAA
Translation:
MGKIIGIDLGTTNSCVAILENGNVKVIENAEGARTTPSIIAYTNDGETLVGQPAKRQAVTNPQNTLYAVKRL
IGRRFEENVVQKDIQMVPYSIVKADNGDAWVEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPA
YFNDSQRQATKDAGRIAGLDVKRIINEPTAAALAYGLDKAKGDHTVIVYDLGGGTFDVSVIEIAEVDGEHQF
EVLATNGDTFLGGEDFDIRLIDYLVDEFKKESGINLKGDPLAMQRLKEAAEKAKIELSSTQQTDVNLPYVTA
DASGPKHLNVKVSRAKLESLVEDLVQRTIEPCRTALKDAGLDVSDIHEVILVGGQTRMPLVQKTVAEFFGKE
ARKDVNPDEAVAVGAAIQGAVLAGDVKDVLLLDVTPLTLGIETLGGVMTGLIEKNTTIPTKKSQVFSTADDN
QGAVTIHVLQGERKQAAQNKSLGKFDLADIPPAPRGVPQIEVTFDIDANGILHVSAKDKATGKQQSIVIKAS
SGLSEDEIQQMVRDAEANAEEDRKFEELAAARNQGDALVHATRKMITEAGDKATAEDKATIEKALGELEAAV
KGDDKAEIEAKMNALSQASTPLAQKMYAEQAQQGEDAPQGEQAKAADDVVDAEFEEVKDNK*
AnnotationID:455GeneID:446
AnnotatorUID: diggins
Modification Date/Time: 2005-05-23 14:02:25
Gene Name:dnaK
Confidence Code:2
GeneProduct:putative heat shock protein
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(9) Chaperones & heat shock proteins
Alternate Gene Product Name:putative molecular chaperone
Functional Category Confidence Code:5
COGs:pfam00012,COG0443
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:






 
Structural Features:
pfam00012, HSP70, Hsp70 protein.
CD-Length = 600 residues, 100.0% aligned
Score =  831 bits (2149), Expect = 0.0 
Identities = 384/607 (63%), Positives = 466/607 (76%), Gaps = 13/607 (2%)

COG0443, DnaK, Molecular chaperone 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 579 residues,  99.5% aligned
Score =  670 bits (1731), Expect = 0.0
Identities = 371/606 (61%), Positives = 455/606 (75%), Gaps = 31/606 (5%)






   
Genomic Context:






   
Comment:


















    ReferenceID:895




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18037




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37650




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:446_cdd.html


    		




 
URL:

















    ReferenceID:38162




  
Author/Investigator(s):
        Farinha MA, Mockett R, Went CJ, Jardine S, Naczynski LM, Kropinski AM.





  
Title:
        Physical mapping of several heat-shock genes in Pseudomonas aeruginosa and the cloning of the mopA (GroEL) gene.




  
PubMed:
        8857035


MedLine:
        




    
Source:
Can J Microbiol. 1996 Apr;42(4):326-34.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8857035&query_hl=23

















    ReferenceID:38163




  
Author/Investigator(s):
        Wetzstein M, Dedio J, Schumann W.





  
Title:
        Complete nucleotide sequence of the Bacillus subtilis dnaK gene.




  
PubMed:
        2110662


MedLine:
        




    
Source:
Nucleic Acids Res. 1990 Apr 25;18(8):2172.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2110662

















    ReferenceID:38164




  
Author/Investigator(s):
        Miyazaki T, Tanaka S, Fujita H, Itikawa H.





  
Title:
        DNA sequence analysis of the dnaK gene of Escherichia coli B and of two dnaK genes carrying the temperature-sensitive mutations dnaK7(Ts) and dnaK756(Ts).




  
PubMed:
        1592823


MedLine:
        




    
Source:
J Bacteriol. 1992 Jun;174(11):3715-22.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1592823









           
Homologs By Global Alignment 

           

       



     
Gene ID:446
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
763
                           PA4761_tr
                           translation of PA4761
                           637
                           99.99
                           99.99
                           0
                           3180.0
     

     
     
33334
                           gb|AAG08147.1|AE004889_10
                           (AE004889) DnaK protein [Pseudomonas aeruginosa PAO1]
                           637
                           99.99
                           99.99
                           0
                           3180.0
     

     
     
33335
                           gb|AAO57953.1|
                           (AE016872) dnaK protein [Pseudomonas syringae pv. tomato str. DC3000]
                           639
                           87.48
                           93.27
                           3
                           2816.0
     

     
     
33336
                           gb|AAN70299.1|AE016670_9
                           (AE016791) dnaK protein [Pseudomonas putida KT2440]
                           641
                           86.73
                           92.97
                           4
                           2799.0
     

     
     
33337
                           gb|AAD31868.1|AF135163_1
                           (AF135163) DnaK protein [Pseudomonas syringae pv. glycinea] [Pseudomonas savastanoi pv. glycinea]
                           640
                           84.68
                           90.31
                           5
                           2692.0
     

     






      

          
GC ORFID: 43448How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4761Glimmer Score: 

          
Start: 4884720Stop: 4882807

          
Length: 1914


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA4761 translation of PA4761Homolog Bit Score 1229.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5477 (ORF: BLASTX 714.0), t_PA3810 (ORF: BLASTX 425.0), psyr_15may02_Scaffold3_revised_gene4521 (ORF: BLASTX 425.0), t_PA4761 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGGCAAAATCATTGGCATCGACCTGGGTACCACCAACTCCTGCGTGGCTATCCTGGAGAACGGTAACGTC 
AAGGTCATCGAGAACGCCGAGGGCGCGCGTACCACCCCCTCGATCATCGCCTACACCAACGATGGCGAAACC 
CTGGTGGGCCAGCCGGCCAAGCGCCAGGCGGTCACCAACCCGCAGAACACCCTGTATGCGGTGAAGCGCCTG 
ATCGGCCGTCGCTTCGAAGAGAACGTGGTACAGAAAGACATCCAGATGGTGCCGTACAGCATCGTCAAGGCC 
GACAACGGCGACGCCTGGGTGGAAGTGAAGGGCCAGAAGATGGCGCCTCCGCAGATTTCCGCCGAAGTGCTG 
AAGAAAATGAAGAAGACCGCCGAAGACTACCTCGGCGAGCCGGTCACCGAAGCGGTGATCACCGTTCCGGCC 
TACTTCAACGACAGCCAGCGCCAGGCCACCAAGGACGCCGGCCGCATCGCCGGCCTCGACGTCAAGCGGATC 
ATCAACGAGCCGACCGCGGCGGCGCTGGCCTACGGCCTGGACAAGGCCAAGGGCGACCACACCGTGATCGTC 
TATGACCTGGGCGGCGGTACCTTCGACGTGTCGGTGATCGAGATCGCCGAAGTCGACGGTGAGCACCAGTTC 
GAAGTGCTGGCCACCAACGGCGACACCTTCCTCGGCGGCGAAGACTTCGACATCCGCCTGATCGACTACCTC 
GTCGACGAGTTCAAGAAGGAAAGCGGCATCAACCTGAAGGGCGACCCGCTGGCCATGCAGCGTCTGAAGGAA 
GCGGCCGAGAAAGCCAAGATCGAGCTGTCCTCGACCCAGCAGACCGACGTCAACCTGCCGTACGTGACTGCC 
GACGCGAGCGGTCCGAAGCACCTGAACGTCAAGGTTTCCCGCGCCAAGCTGGAGTCCCTGGTCGAAGACCTG 
GTGCAGCGCACCATCGAGCCGTGCCGCACCGCGCTGAAGGATGCCGGCCTGGATGTTTCCGATATCCATGAA 
GTGATCCTGGTCGGCGGCCAGACCCGCATGCCGCTGGTACAGAAGACCGTTGCCGAGTTCTTCGGCAAGGAA 
GCGCGCAAGGACGTCAACCCGGACGAAGCCGTGGCTGTCGGTGCCGCCATCCAGGGCGCGGTCCTGGCCGGC 
GACGTGAAGGACGTGCTGCTGCTCGACGTGACCCCGCTGACCCTCGGCATCGAGACCTTGGGCGGCGTGATG 
ACCGGTCTGATCGAGAAGAACACCACCATCCCGACCAAGAAGTCGCAGGTGTTCTCCACCGCCGACGACAAC 
CAGGGCGCGGTGACCATCCACGTGCTGCAGGGCGAGCGCAAGCAGGCCGCGCAGAACAAGTCGCTGGGCAAG 
TTCGACCTGGCCGACATTCCGCCGGCTCCGCGCGGCGTGCCGCAGATCGAGGTGACCTTCGATATCGATGCC 
AACGGCATCCTGCACGTCTCGGCGAAGGACAAGGCCACTGGCAAGCAGCAGTCCATCGTGATCAAGGCATCC 
TCCGGCCTGTCCGAGGATGAGATCCAGCAGATGGTCCGCGATGCCGAGGCGAACGCCGAGGAGGACCGCAAG 
TTCGAGGAACTGGCTGCCGCTCGCAACCAGGGCGACGCGCTGGTCCACGCGACCCGCAAGATGATCACCGAG 
GCGGGCGACAAGGCCACCGCCGAGGACAAGGCGACCATCGAGAAGGCGCTGGGCGAGCTGGAAGCGGCGGTG 
AAGGGCGACGACAAGGCCGAGATCGAGGCCAAGATGAACGCTCTGTCCCAGGCTTCCACCCCGCTGGCGCAG 
AAGATGTACGCCGAACAGGCCCAGCAGGGCGAAGACGCTCCCCAGGGCGAGCAGGCGAAAGCCGCTGACGAC 
GTGGTGGACGCCGAGTTCGAAGAGGTCAAGGACAACAAGTAA