ViewGene
Locus Name:
GeneID:
Location:

GeneID:4456
TrivialName: PA14_28450
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_28450
PAO1 Ortholog Locus:PA2755
Sequence Length:471
Protein Length:156
Start: 2457319
Stop: 2456849
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06143.1|AE004703_9 (AE004703) ecotin precursor [Pseudomonas aeruginosa PAO1]
Identities = 154/156 (98%), Positives = 154/156 (98%)
Sequence:
ATGAAAGCACTACTGATCGCCGCCGGCGTTGCCGCTCTTTCCAGCACTGCCATGGCCGCCAAACTGGATGAA
AAGGTTCCCTACCCGAAGGCAGATGCCGGCTTCACCCGCCAGGTCATCCACCTGCCGAAACAGGACACGGAA
GACGCATTCAAGGTCGAGATCATCGCCGGCAAGACCCTCGAGGCCGACTGCAACCAGCAGCGCCTGGGCGGC
GAGCTGGAAGAACACACCCTGGAAGGCTGGGGCTACTCCTACTACCGCCTGGACAAGGTCAGCGGGCCGATG
AGCACGATGATGGCCTGCCCCGGCCAGAAGAAGGAGCAGCGCTTCATCCCGGTGGTCGGCGAAGGCTTCCTG
CTGCGCTACAACAGCAAGCTGCCGATCGTGGTCTATGCGCCGAAGGACGTGGAGGTCCGCTATCGGATCTGG
TCAGCGTCCGAAAAGGTCGAGAAAGCGGTCAGCGAATAA
Translation:
MKALLIAAGVAALSSTAMAAKLDEKVPYPKADAGFTRQVIHLPKQDTEDAFKVEIIAGKTLEADCNQQRLGG
ELEEHTLEGWGYSYYRLDKVSGPMSTMMACPGQKKEQRFIPVVGEGFLLRYNSKLPIVVYAPKDVEVRYRIW
SASEKVEKAVSE*
AnnotationID:4462GeneID:4456
AnnotatorUID: diggins
Modification Date/Time: 2005-04-05 13:01:48
Gene Name:eco
Confidence Code:2
GeneProduct:ecotin precursor
Cell Localization:()
Synonyms:ecotin
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:eti
Functional Category Confidence Code:5
COGs:cd00242
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN68680.1|AE016497_5 (AE016785) ecotin [Pseudomonas putida KT2440]
          Length = 159

 Score =  220 bits (561), Expect = 2e-57
 Identities = 104/152 (68%), Positives = 126/152 (82%), Gaps = 1/152 (0%)

>gb|AAN81204.1|AE016763_163 (AE016763) Ecotin precursor [Escherichia coli CFT073]
          Length = 169

 Score =  173 bits (439), Expect = 3e-43
 Identities = 88/160 (55%), Positives = 114/160 (71%), Gaps = 6/160 (3%)

>gb|AAG57344.1|AE005453_1 (AE005453) ecotin, a serine protease inhibitor [Escherichia coli
           O157:H7 EDL933]
          Length = 162

 Score =  172 bits (437), Expect = 5e-43
 Identities = 88/160 (55%), Positives = 114/160 (71%), Gaps = 6/160 (3%)






 
Structural Features:
cd00242, Ecotin, Protease Inhibitor Ecotin
CD-Length = 136 residues, 100.0% aligned
Score =  222 bits (566), Expect = 2e-59
Identities = 85/136 (62%), Positives = 99/136 (72%)






   
Genomic Context:






   
Comment:


















    ReferenceID:8913




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:26055




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36300




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4456_cdd.html


    		




 
URL:

















    ReferenceID:36393




  
Author/Investigator(s):
        Lee HR, Seo JH, Kim OM, Lee CS, Suh SW, Hong YM, Tanaka K, Ichihara A, Ha DB, Chung CH.





  
Title:
        Molecular cloning of the ecotin gene in Escherichia coli.




  
PubMed:
        1879537


MedLine:
        




    
Source:
FEBS Lett. 1991 Aug 5;287(1-2):53-6.

Erratum in:
    * FEBS Lett 1992 Jan 20;296(2):235.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1879537

















    ReferenceID:36394




  
Author/Investigator(s):
        McGrath ME, Hines WM, Sakanari JA, Fletterick RJ, Craik CS.





  
Title:
        The sequence and reactive site of ecotin. A general inhibitor of pancreatic serine proteases from Escherichia coli.




  
PubMed:
        2007606


MedLine:
        




    
Source:
J Biol Chem. 1991 Apr 5;266(10):6620-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2007606

















    ReferenceID:36395




  
Author/Investigator(s):
        Erpel T, Hwang P, Craik CS, Fletterick RJ, McGrath ME.





  
Title:
        Physical map location of the new Escherichia coli gene eco, encoding the serine protease inhibitor ecotin.




  
PubMed:
        1537814


MedLine:
        




    
Source:
J Bacteriol. 1992 Mar;174(5):1704.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1537814









           
Homologs By Global Alignment 

           

       



     
Gene ID:4456
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6799
                           PA2755_tr
                           translation of PA2755
                           156
                           98.71
                           98.71
                           0
                           788.0
     

     
     
182095
                           gb|AAG06143.1|AE004703_9
                           (AE004703) ecotin precursor [Pseudomonas aeruginosa PAO1]
                           156
                           98.71
                           98.71
                           0
                           788.0
     

     
     
182096
                           gb|AAN68680.1|AE016497_5
                           (AE016785) ecotin [Pseudomonas putida KT2440]
                           161
                           66.45
                           79.50
                           7
                           563.0
     

     
     
182104
                           dbj|BAB36521.1|
                           (AP002560) ecotin, a serine protease inhibitor [Escherichia coli O157:H7]
                           162
                           54.32
                           70.37
                           6
                           442.0
     

     
     
182103
                           gb|AAG57344.1|AE005453_1
                           (AE005453) ecotin, a serine protease inhibitor [Escherichia coli O157:H7 EDL933]
                           162
                           54.32
                           70.37
                           6
                           442.0
     

     






      

          
GC ORFID: 47458How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2755Glimmer Score: 

          
Start: 2496607Stop: 2497077

          
Length: 471


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA2755 translation of PA2755Homolog Bit Score 308.0

          
Other Homologs:  t_PA2755 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAAGCACTACTGATCGCCGCCGGCGTTGCCGCTCTTTCCAGCACTGCCATGGCCGCCAAACTGGATGAA 
AAGGTTCCCTACCCGAAGGCAGATGCCGGCTTCACCCGCCAGGTCATCCACCTGCCGAAACAGGACACGGAA 
GACGCATTCAAGGTCGAGATCATCGCCGGCAAGACCCTCGAGGCCGACTGCAACCAGCAGCGCCTGGGCGGC 
GAGCTGGAAGAACACACCCTGGAAGGCTGGGGCTACTCCTACTACCGCCTGGACAAGGTCAGCGGGCCGATG 
AGCACGATGATGGCCTGCCCCGGCCAGAAGAAGGAGCAGCGCTTCATCCCGGTGGTCGGCGAAGGCTTCCTG 
CTGCGCTACAACAGCAAGCTGCCGATCGTGGTCTATGCGCCGAAGGACGTGGAGGTCCGCTATCGGATCTGG 
TCAGCGTCCGAAAAGGTCGAGAAAGCGGTCAGCGAATAA