ViewGene
Locus Name:
GeneID:
Location:

GeneID:4442
TrivialName: PA14_02230
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_02230
PAO1 Ortholog Locus:PA0177
Sequence Length:486
Protein Length:161
Start: 201020
Stop: 200535
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03567.1|AE004455_8 (AE004455) probable purine-binding chemotaxis protein [Pseudomonas aeruginosa PAO1]
Identities = 161/161 (100%), Positives = 161/161 (100%)
Sequence:
ATGAACGGCACTGAGGCAAGCCAGGCGAACGGCCCGGCGCAGGAATACCTGACCTTCACCCTGGGCCGCGAG
GAGTACGCCATCGACATCCTCCGCGTGCAGGAGATCCGCGGCTACGACCAGGTCACCGCGATCGCCAACGCG
CCGCCCTTCATCAAGGGCGTGATCAACCTGCGCGGGGCGATCGTGCCGATCGTCGACCTGCGCATCAAGTTC
CACCTCGCCGAGGTCACCTACGACCCGTTCACCGTGGTGATCATCCTCAACATCGGCCGGCGCATCGTCGGC
GTGGTGGTGGACTCGGTATCCGACGTGATCGCCCTGGGCGGCGACGCGATCAGGCCGCCGCCGGAGTTCGGC
GCCAGCTTCGACACCGAATACCTGCTCGGCCTGGCCACCGCGGGCGAGCGCATGTTGATCCTGGTGGACATC
GAGAGACTGATGACCAGCCGCGAAATGGCGCTGGTCGACGAAGCCGCGGCCTGA
Translation:
MNGTEASQANGPAQEYLTFTLGREEYAIDILRVQEIRGYDQVTAIANAPPFIKGVINLRGAIVPIVDLRIKF
HLAEVTYDPFTVVIILNIGRRIVGVVVDSVSDVIALGGDAIRPPPEFGASFDTEYLLGLATAGERMLILVDI
ERLMTSREMALVDEAAA*
AnnotationID:4448GeneID:4442
AnnotatorUID: diggins
Modification Date/Time: 2005-05-08 15:03:45
Gene Name:cheW
Confidence Code:2
GeneProduct:putative purine-binding chemotaxis protein
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(10) Chemotaxis
Alternate Gene Product Name:putative positive regulator of CheA protein activity
Functional Category Confidence Code:5
COGs:COG0835,cd00732,pfam01584
Secondary Functional Category(ies):two-component regulatory system
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAK13043.1| (AY026063) chemotaxis protein CheW [Burkholderia mallei]
          Length = 175

 Score =  204 bits (518), Expect = 2e-52
 Identities = 104/157 (66%), Positives = 119/157 (75%)

>emb|CAE33041.1| (BX640444) chemotaxis protein CheW [Bordetella bronchiseptica]
          Length = 166

 Score =  201 bits (512), Expect = 1e-51
 Identities = 106/160 (66%), Positives = 123/160 (76%), Gaps = 2/160 (1%)

>gb|AAP17319.1| (AE016984) positive regulator of CheA protein activity [Shigella
           flexneri 2a str. 2457T]
          Length = 167

 Score =  199 bits (506), Expect = 5e-51
 Identities = 102/156 (65%), Positives = 123/156 (78%), Gaps = 2/156 (1%)








 
Structural Features:
COG0835, CheW, Chemotaxis signal transduction protein 
[Cell motility and secretion / Signal transduction mechanisms]
CD-Length = 165 residues,  95.2% aligned
Score =  148 bits (375), Expect = 3e-37
Identities = 65/157 (41%), Positives = 95/157 (60%)

cd00732, CheW, CheW, a small regulator protein, unique to the chemotaxis signalling 
in prokaryotes and archea.
CD-Length = 140 residues,  99.3% aligned
Score =  143 bits (363), Expect = 7e-36
Identities = 58/139 (41%), Positives = 92/139 (66%)

pfam01584, CheW, CheW-like domain. CheW proteins are part of the 
chemotaxis signaling mechanism in bacteria. 
CD-Length = 141 residues,  99.3% aligned
Score =  128 bits (323), Expect = 3e-31
Identities = 59/140 (42%), Positives = 92/140 (65%), Gaps = 1/140 (0%)







   
Genomic Context:






   
Comment:


















    ReferenceID:8885




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:26028




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33549




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4442_cdd.html


    		




 
URL:

















    ReferenceID:35487




  
Author/Investigator(s):
        Kato J, Nakamura T, Kuroda A, Ohtake H.





  
Title:
        Cloning and characterization of chemotaxis genes in Pseudomonas aeruginosa.




  
PubMed:
        10052136


MedLine:
        




    
Source:
Biosci Biotechnol Biochem. 1999 Jan;63(1):155-61.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10052136









           
Homologs By Global Alignment 

           

       



     
Gene ID:4442
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6785
                           PA0177_tr
                           translation of PA0177
                           161
                           99.99
                           99.99
                           0
                           797.0
     

     
     
181688
                           gb|AAG03567.1|AE004455_8
                           (AE004455) probable purine-binding chemotaxis protein [Pseudomonas aeruginosa PAO1]
                           161
                           99.99
                           99.99
                           0
                           797.0
     

     
     
181689
                           gb|AAQ61102.1|
                           (AE016922) purine-binding chemotaxis protein [Chromobacterium violaceum ATCC 12472]
                           166
                           65.06
                           76.50
                           5
                           528.0
     

     
     
181694
                           emb|CAE41329.1|
                           (BX640414) chemotaxis protein CheW [Bordetella pertussis] [Bordetella pertussis Tohama I]
                           166
                           63.85
                           74.09
                           5
                           509.0
     

     
     
181692
                           emb|CAE33041.1|
                           (BX640444) chemotaxis protein CheW [Bordetella bronchiseptica] [Bordetella bronchiseptica RB50]
                           166
                           63.85
                           74.09
                           5
                           509.0
     

     






      

          
GC ORFID: 47444How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0177Glimmer Score: 

          
Start: 6002359Stop: 6001874

          
Length: 486


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0177 translation of PA0177Homolog Bit Score 311.0

          
Other Homologs:  t_PA0177 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACGGCACTGAGGCAAGCCAGGCGAACGGCCCGGCGCAGGAATACCTGACCTTCACCCTGGGCCGCGAG 
GAGTACGCCATCGACATCCTCCGCGTGCAGGAGATCCGCGGCTACGACCAGGTCACCGCGATCGCCAACGCG 
CCGCCCTTCATCAAGGGCGTGATCAACCTGCGCGGGGCGATCGTGCCGATCGTCGACCTGCGCATCAAGTTC 
CACCTCGCCGAGGTCACCTACGACCCGTTCACCGTGGTGATCATCCTCAACATCGGCCGGCGCATCGTCGGC 
GTGGTGGTGGACTCGGTATCCGACGTGATCGCCCTGGGCGGCGACGCGATCAGGCCGCCGCCGGAGTTCGGC 
GCCAGCTTCGACACCGAATACCTGCTCGGCCTGGCCACCGCGGGCGAGCGCATGTTGATCCTGGTGGACATC 
GAGAGACTGATGACCAGCCGCGAAATGGCGCTGGTCGACGAAGCCGCGGCCTGA