ViewGene
Locus Name:
GeneID:
Location:

GeneID:4435
TrivialName: PA14_45500
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_45500
PAO1 Ortholog Locus:PA1464
Sequence Length:480
Protein Length:159
Start: 4050127
Stop: 4049648
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04853.1|AE004576_2 (AE004576) probable purine-binding chemotaxis protein [Pseudomonas aeruginosa PAO1]
Identities = 159/159 (100%), Positives = 159/159 (100%)
Sequence:
ATGAGCAAAGCCACCGCGCAAAGCGCCGAAGATCCGATCCTGCAGTGGGTCACCTTCCGCCTGGACAACGAG
AGCTACGGCATCAACGTGATGCAGGTCCAGGAGGTGCTGCGCTACACCGAGATCGCCCCGGTGCCGGGCGCG
CCGAGCTACGTGCTGGGCATCATCAACCTGCGCGGCAACGTGGTCACGGTGATCGACACCCGGCAGCGCTTC
GGCCTGGACCCTGCGCCGGTCAGCGACAACACGCGGATCGTCATCATCGAGGCGGACAAGCAGGTGGTCGGC
ATCCTGGTCGACAGCGTCGCCGAGGTGGTCTACCTGAAGCAGTCGGAAATCGAGACCGCGCCGAACGTCGGT
AACGAAGAATCGGCCAAGTTCATCCAGGGCGTCTGCAACAAGAACGGCGAGCTGCTGATCCTGGTCGAGCTG
GACAAGATGATGACCGAGGAAGAATGGTCGGAGCTGGGCAGCATCTGA
Translation:
MSKATAQSAEDPILQWVTFRLDNESYGINVMQVQEVLRYTEIAPVPGAPSYVLGIINLRGNVVTVIDTRQRF
GLDPAPVSDNTRIVIIEADKQVVGILVDSVAEVVYLKQSEIETAPNVGNEESAKFIQGVCNKNGELLILVEL
DKMMTEEEWSELGSI*
AnnotationID:4441GeneID:4435
AnnotatorUID: diggins
Modification Date/Time: 2005-02-24 13:01:22
Gene Name:
Confidence Code:3
GeneProduct:putative purine-binding chemotaxis protein CheW
Cell Localization:()
Synonyms:cheW
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(10) Chemotaxis
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:cd00732,COG0835,pfam01584
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO55506.1| (AE016862) chemotaxis protein CheW [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 159

 Score =  289 bits (740), Expect = 4e-78
 Identities = 146/159 (91%), Positives = 154/159 (96%)

>gb|AAN69911.1|AE016630_2 (AE016790) purine-binding chemotaxis protein CheW [Pseudomonas
           putida KT2440]
          Length = 159

 Score =  289 bits (740), Expect = 4e-78
 Identities = 147/159 (92%), Positives = 153/159 (96%)

>gb|AAN56201.1|AE015758_5 (AE015758) purine-binding chemotaxis protein CheW [Shewanella
           oneidensis MR-1]
          Length = 164

 Score =  245 bits (626), Expect = 6e-65
 Identities = 121/156 (77%), Positives = 140/156 (89%)






 
Structural Features:
cd00732, CheW, CheW, a small regulator protein, unique to the 
chemotaxis signalling in prokaryotes and archea.
CD-Length = 140 residues, 100.0% aligned
Score =  163 bits (414), Expect = 1e-41
Identities = 76/140 (54%), Positives = 103/140 (73%)

COG0835, CheW, Chemotaxis signal transduction protein 
[Cell motility and secretion / Signal transduction mechanisms]
CD-Length = 165 residues,  95.8% aligned
Score =  153 bits (387), Expect = 1e-38
Identities = 57/158 (36%), Positives = 105/158 (66%)

pfam01584, CheW, CheW-like domain.
CD-Length = 141 residues, 100.0% aligned
Score =  141 bits (356), Expect = 5e-35
Identities = 63/141 (44%), Positives = 96/141 (68%), Gaps = 1/141 (0%)









   
Genomic Context:






   
Comment:


















    ReferenceID:8871




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:26014




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37377




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4435_cdd.html


    		




 
URL:

















    ReferenceID:37961




  
Author/Investigator(s):
        Kato J, Nakamura T, Kuroda A, Ohtake H.





  
Title:
        Cloning and characterization of chemotaxis genes in Pseudomonas aeruginosa.




  
PubMed:
        10052136


MedLine:
        




    
Source:
Biosci Biotechnol Biochem. 1999 Jan;63(1):155-61.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10052136









           
Homologs By Global Alignment 

           

       



     
Gene ID:4435
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6776
                           PA1464_tr
                           translation of PA1464
                           159
                           99.99
                           99.99
                           0
                           796.0
     

     
     
181397
                           gb|AAG04853.1|AE004576_2
                           (AE004576) probable purine-binding chemotaxis protein [Pseudomonas aeruginosa PAO1]
                           159
                           99.99
                           99.99
                           0
                           796.0
     

     
     
181398
                           dbj|BAA33555.1|
                           (AB012767) CheW [Pseudomonas aeruginosa]
                           159
                           99.99
                           99.99
                           0
                           796.0
     

     
     
181400
                           gb|AAN69911.1|AE016630_2
                           (AE016790) purine-binding chemotaxis protein CheW [Pseudomonas putida KT2440]
                           159
                           92.45
                           96.22
                           0
                           740.0
     

     
     
181401
                           gb|AAC08070.1|
                           (AF031898) CheW [Pseudomonas putida]
                           159
                           92.45
                           96.22
                           0
                           740.0
     

     






      

          
GC ORFID: 47437How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1464Glimmer Score: 

          
Start: 903782Stop: 904261

          
Length: 480


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1464 translation of PA1464Homolog Bit Score 311.0

          
Other Homologs:  psyr_15may02_Scaffold8_revised_gene7948 (ORF: BLASTX 288.0), t_PA1464 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCAAAGCCACCGCGCAAAGCGCCGAAGATCCGATCCTGCAGTGGGTCACCTTCCGCCTGGACAACGAG 
AGCTACGGCATCAACGTGATGCAGGTCCAGGAGGTGCTGCGCTACACCGAGATCGCCCCGGTGCCGGGCGCG 
CCGAGCTACGTGCTGGGCATCATCAACCTGCGCGGCAACGTGGTCACGGTGATCGACACCCGGCAGCGCTTC 
GGCCTGGACCCTGCGCCGGTCAGCGACAACACGCGGATCGTCATCATCGAGGCGGACAAGCAGGTGGTCGGC 
ATCCTGGTCGACAGCGTCGCCGAGGTGGTCTACCTGAAGCAGTCGGAAATCGAGACCGCGCCGAACGTCGGT 
AACGAAGAATCGGCCAAGTTCATCCAGGGCGTCTGCAACAAGAACGGCGAGCTGCTGATCCTGGTCGAGCTG 
GACAAGATGATGACCGAGGAAGAATGGTCGGAGCTGGGCAGCATCTGA