ViewGene
Locus Name:
GeneID:
Location:

GeneID:442
TrivialName: PA14_37790
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_37790
PAO1 Ortholog Locus:PA2065
Sequence Length:1821
Protein Length:606
Start: 3369223
Stop: 3371043
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05453.1|AE004633_4 (AE004633) copper resistance protein A precursor [Pseudomonas aeruginosa PAO1]
Identities = 604/632 (95%), Positives = 605/632 (95%), Gaps = 26/632 (4%)
Sequence:
ATGCACAGAACTTCGCGACGCACCTTCGTCAAAGGCCTCGCCGCCACCGGTCTGCTCGGCGGACTGGGTCTC
TGGCGCGCCCCGGCCTGGGCCCTGGCCGGGCCGGGCCAGCAGAACCTGCTCGCCGGCGACAGTTTCGACCTG
TTCATCGGCGAGACCCCGGTGAACCTCAGCGGCTCGCCGGCCACCGCCATGACCATCAACGGTTCGCTGCCC
GGCCCGACCCTGCGCTGGCGCGAAGGCGACAACGTGACCCTGCGGGTGCGCAACCGCCTCGCCGAGGACACC
TCGATCCACTGGCACGGCATCATCCTGCCGGCCAACATGGATGGCGTGCCGGGCCTCAGCTTCGAAGGCATC
GCTCCCGGCGGTCTCTACGAATACCGCTTCAAGGTCCGGCAGAACGGCACCTACTGGTACCACAGCCACTCG
GGGCTACAGGAGCAGGCCGGGGTCTACGGCGCCCTGGTGATCGACGCCCGCGAGCCGGAACCGTTCAGCTAC
GACCGCGACTACGTGGTGCTGCTCAGCGACTGGTCGGACGAGAAGCCGCAGCGCATCCTCGCCAAGCTGAAG
AAGCAGTCCGACTACTACAACTTCCACAAGCGCACCGTCGGCGACTTCATCGACGACGTCAGCGCCAACGGC
TGGGCCGCGACCCTGGCCGACCGCAAGATGTGGGCCGAGATGAAGATGAGTCCCACCGATCTCGCCGACGTC
AGCGGCTACACCTATACCTACCTGCTCAACGGACAGCCGCCGGACGGCAACTGGACCGGCCTGTTCCGCCCC
GGCGAAAAGCTCCGCCTGCGCTTCGTCAACGCCTCGGCGATGAGCTATTTCGACGTCCGCATTCCCGGCCTG
AAGATGACCGTGGTGGCCGCCGACGGACAGCACGTCGAGCCGGTCAGCGTCGACGAGTTGCGCATCGCCGTG
GCCGAGACCTACGACGTGATCGTCGAGCCGGGCGGCGAGCGCGCCTATACCCTCTTCGCCCAGTCCATGGAC
CGCAGCGGCTACGCCCGCGGCACCCTGGCGCTGGCCGAAGGGCTTAGCGCGCCGGTGCCGACGCCCGATCCG
CGCCCGCTGATCGGCATGGACGACATGGGCATGGGCGGCATGGACCACGGCGCCATGGGGCATGGCGCGGCG
ACGAGGCCCGCCAGCGAGATGGACCATTCGAAGATGTCGGGCATGGACATGAACGGCATGGACCACTCGAAG
ATGGCCGGCATGGACATGAGCGGCATGGACCATTCCAGGATGGGCATGGGCGCCATGCCGATGCAGTCCCAT
CCGGCTTCGGAGGACGGCAACCCGCTGGTCGACATGCAGACCATGACGCCGACACCGAAGCTCGCCGACCCC
GGCCTCGGCCTGCGCGACAACGGGCGGCGGGTGCTGACCTACGCCGACCTGCGCAGCCGCTTCGCCGACCCG
GACGGCCGCGAGCCGGGGCGCACCATCGAGCTGCACCTGACCGGGCACATGGAGAAGTTCGCCTGGTCCTTC
GACGGCATCAAGTTCTCCGACGCCGAGCCGCTGCGCCTGACCTACGGCGAGCGCCTGCGGATCGTCCTGGTC
AACGACACCATGATGACCCACCCGATCCACCTGCACGGCATGTGGAGCGACCTGGAGGACGAGCAAGGCAAC
TTCCTGGTGCGCAAGCACACCATCGACATGCCGCCGGGCACCCGCCGCAGCTACCGGGTCACCGCCGACGCC
CTGGGGCGCTGGGCCTACCACTGCCACCTGCTGTTCCACATGGAGATGGGCATGTTCCGCGAAGTGCGGGTA
GACGAAGGAGACAACGCATGA
Translation:
MHRTSRRTFVKGLAATGLLGGLGLWRAPAWALAGPGQQNLLAGDSFDLFIGETPVNLSGSPATAMTINGSLP
GPTLRWREGDNVTLRVRNRLAEDTSIHWHGIILPANMDGVPGLSFEGIAPGGLYEYRFKVRQNGTYWYHSHS
GLQEQAGVYGALVIDAREPEPFSYDRDYVVLLSDWSDEKPQRILAKLKKQSDYYNFHKRTVGDFIDDVSANG
WAATLADRKMWAEMKMSPTDLADVSGYTYTYLLNGQPPDGNWTGLFRPGEKLRLRFVNASAMSYFDVRIPGL
KMTVVAADGQHVEPVSVDELRIAVAETYDVIVEPGGERAYTLFAQSMDRSGYARGTLALAEGLSAPVPTPDP
RPLIGMDDMGMGGMDHGAMGHGAATRPASEMDHSKMSGMDMNGMDHSKMAGMDMSGMDHSRMGMGAMPMQSH
PASEDGNPLVDMQTMTPTPKLADPGLGLRDNGRRVLTYADLRSRFADPDGREPGRTIELHLTGHMEKFAWSF
DGIKFSDAEPLRLTYGERLRIVLVNDTMMTHPIHLHGMWSDLEDEQGNFLVRKHTIDMPPGTRRSYRVTADA
LGRWAYHCHLLFHMEMGMFREVRVDEGDNA*
AnnotationID:451GeneID:442
AnnotatorUID: danlee
Modification Date/Time: 2006-03-21 18:06:21
Gene Name:pcoA
Confidence Code:2
GeneProduct: copper resistance protein A precursor
Cell Localization:()
Synonyms:copA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(1) Adaptation, protection
Alternate Gene Product Name:copper-binding protein
Functional Category Confidence Code:5
COGs:COG2132,pfam00394
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70945.1|AE016738_7 (AE016794) copper resistance protein A [Pseudomonas putida KT2440]
          Length = 669

 Score =  999 bits (2584), Expect = 0.0
 Identities = 490/666 (73%), Positives = 543/666 (81%), Gaps = 66/666 (9%)

>gb|AAN52531.1|AF455752_2 (AF455752) potential multicopper oxidase [Klebsiella pneumoniae]
          Length = 605

 Score =  901 bits (2328), Expect = 0.0
 Identities = 445/619 (71%), Positives = 498/619 (80%), Gaps = 36/619 (5%)

>gb|AAM39893.1| (AE012155) copper resistance protein A precursor [Xanthomonas
           campestris pv. campestris str. ATCC 33913]
          Length = 602

 Score =  757 bits (1954), Expect = 0.0
 Identities = 374/599 (62%), Positives = 442/599 (73%), Gaps = 11/599 (1%)








 
Structural Features:
COG2132, SufI, Putative multicopper oxidases 
[Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 451 residues, only  77.6% aligned
Score =  229 bits (585), Expect = 7e-61
Identities = 120/407 (29%), Positives = 160/407 (39%), Gaps = 63/407 (15%)

pfam00394, Cu-oxidase, Multicopper oxidase. 
CD-Length = 155 residues,  93.5% aligned
Score = 87.3 bits (216), Expect = 5e-18
Identities = 40/155 (25%), Positives = 57/155 (36%), Gaps = 23/155 (14%)






   
Genomic Context:






   
Comment:
Similar to PA2065 (Identities = 604/632, 95%).  
Has an internal deletion of 26 amino acids as compared to PA2065.


















    ReferenceID:887




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18029




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32636




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:442_cdd.html


    		




 
URL:

















    ReferenceID:32637




  
Author/Investigator(s):
        Huston WM, Jennings MP, McEwan AG.





  
Title:
        The multicopper oxidase of Pseudomonas aeruginosa is a ferroxidase with a central role in iron acquisition.




  
PubMed:
        12354238


MedLine:
        22242525




    
Source:
Mol Microbiol. 2002 Sep;45(6):1741-50.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=2224

















    ReferenceID:34411




  
Author/Investigator(s):
        Cha JS, Cooksey DA.





  
Title:
        Copper resistance in Pseudomonas syringae mediated by periplasmic and outer membrane proteins.




  
PubMed:
        1924351


MedLine:
        




    
Source:
Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):8915-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1924351

















    ReferenceID:34412




  
Author/Investigator(s):
        Francki KT, Chang BJ, Mee BJ, Collignon PJ, Susai V, Keese PK.





  
Title:
        Identification of genes associated with copper tolerance in an adhesion-defective mutant of Aeromonas veronii biovar sobria.




  
PubMed:
        11024350


MedLine:
        




    
Source:
FEMS Immunol Med Microbiol. 2000 Oct;29(2):115-21.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11024350

















    ReferenceID:34413




  
Author/Investigator(s):
        Silver S, Ji G.





  
Title:
        Newer systems for bacterial resistances to toxic heavy metals.




  
PubMed:
        7843081


MedLine:
        




    
Source:
Environ Health Perspect. 1994 Sep;102 Suppl 3:107-13.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7843081









           
Homologs By Global Alignment 

           

       



     
Gene ID:442
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
33293
                           gb|AAN52530.1|AF455751_1
                           (AF455751) multicopper oxidase [Pseudomonas aeruginosa]
                           606
                           96.86
                           97.02
                           13
                           3132.0
     

     
     
758
                           PA2065_tr
                           translation of PA2065
                           632
                           95.56
                           95.72
                           26
                           3215.0
     

     
     
33292
                           gb|AAG05453.1|AE004633_4
                           (AE004633) copper resistance protein A precursor [Pseudomonas aeruginosa PAO1]
                           632
                           95.56
                           95.72
                           26
                           3215.0
     

     
     
33296
                           gb|AAA25806.1|
                           (M19930) copA peptide [Pseudomonas syringae]
                           625
                           73.92
                           82.08
                           35
                           2450.0
     

     
     
33297
                           gb|AAO57380.1|
                           (AE016870) copper resistance protein A [Pseudomonas syringae pv. tomato str. DC3000]
                           608
                           73.68
                           83.88
                           21
                           2420.0
     

     






      

          
GC ORFID: 43444How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2065Glimmer Score: 

          
Start: 1584690Stop: 1582870

          
Length: 1821


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2065 translation of PA2065Homolog Bit Score 1237.0

          
Other Homologs:  psyr_15may02_Scaffold10_revised_gene1697 (ORF: BLASTX 938.0), psyr_15may02_Scaffold10_revised_gene1538 (ORF: BLASTX 934.0), t_PA2065 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCACAGAACTTCGCGACGCACCTTCGTCAAAGGCCTCGCCGCCACCGGTCTGCTCGGCGGACTGGGTCTC 
TGGCGCGCCCCGGCCTGGGCCCTGGCCGGGCCGGGCCAGCAGAACCTGCTCGCCGGCGACAGTTTCGACCTG 
TTCATCGGCGAGACCCCGGTGAACCTCAGCGGCTCGCCGGCCACCGCCATGACCATCAACGGTTCGCTGCCC 
GGCCCGACCCTGCGCTGGCGCGAAGGCGACAACGTGACCCTGCGGGTGCGCAACCGCCTCGCCGAGGACACC 
TCGATCCACTGGCACGGCATCATCCTGCCGGCCAACATGGATGGCGTGCCGGGCCTCAGCTTCGAAGGCATC 
GCTCCCGGCGGTCTCTACGAATACCGCTTCAAGGTCCGGCAGAACGGCACCTACTGGTACCACAGCCACTCG 
GGGCTACAGGAGCAGGCCGGGGTCTACGGCGCCCTGGTGATCGACGCCCGCGAGCCGGAACCGTTCAGCTAC 
GACCGCGACTACGTGGTGCTGCTCAGCGACTGGTCGGACGAGAAGCCGCAGCGCATCCTCGCCAAGCTGAAG 
AAGCAGTCCGACTACTACAACTTCCACAAGCGCACCGTCGGCGACTTCATCGACGACGTCAGCGCCAACGGC 
TGGGCCGCGACCCTGGCCGACCGCAAGATGTGGGCCGAGATGAAGATGAGTCCCACCGATCTCGCCGACGTC 
AGCGGCTACACCTATACCTACCTGCTCAACGGACAGCCGCCGGACGGCAACTGGACCGGCCTGTTCCGCCCC 
GGCGAAAAGCTCCGCCTGCGCTTCGTCAACGCCTCGGCGATGAGCTATTTCGACGTCCGCATTCCCGGCCTG 
AAGATGACCGTGGTGGCCGCCGACGGACAGCACGTCGAGCCGGTCAGCGTCGACGAGTTGCGCATCGCCGTG 
GCCGAGACCTACGACGTGATCGTCGAGCCGGGCGGCGAGCGCGCCTATACCCTCTTCGCCCAGTCCATGGAC 
CGCAGCGGCTACGCCCGCGGCACCCTGGCGCTGGCCGAAGGGCTTAGCGCGCCGGTGCCGACGCCCGATCCG 
CGCCCGCTGATCGGCATGGACGACATGGGCATGGGCGGCATGGACCACGGCGCCATGGGGCATGGCGCGGCG 
ACGAGGCCCGCCAGCGAGATGGACCATTCGAAGATGTCGGGCATGGACATGAACGGCATGGACCACTCGAAG 
ATGGCCGGCATGGACATGAGCGGCATGGACCATTCCAGGATGGGCATGGGCGCCATGCCGATGCAGTCCCAT 
CCGGCTTCGGAGGACGGCAACCCGCTGGTCGACATGCAGACCATGACGCCGACACCGAAGCTCGCCGACCCC 
GGCCTCGGCCTGCGCGACAACGGGCGGCGGGTGCTGACCTACGCCGACCTGCGCAGCCGCTTCGCCGACCCG 
GACGGCCGCGAGCCGGGGCGCACCATCGAGCTGCACCTGACCGGGCACATGGAGAAGTTCGCCTGGTCCTTC 
GACGGCATCAAGTTCTCCGACGCCGAGCCGCTGCGCCTGACCTACGGCGAGCGCCTGCGGATCGTCCTGGTC 
AACGACACCATGATGACCCACCCGATCCACCTGCACGGCATGTGGAGCGACCTGGAGGACGAGCAAGGCAAC 
TTCCTGGTGCGCAAGCACACCATCGACATGCCGCCGGGCACCCGCCGCAGCTACCGGGTCACCGCCGACGCC 
CTGGGGCGCTGGGCCTACCACTGCCACCTGCTGTTCCACATGGAGATGGGCATGTTCCGCGAAGTGCGGGTA 
GACGAAGGAGACAACGCATGA