ViewGene
Locus Name:
GeneID:
Location:

GeneID:4407
TrivialName: PA14_29900
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_29900
PAO1 Ortholog Locus:PA2645
Sequence Length:501
Protein Length:166
Start: 2589903
Stop: 2589403
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06033.1|AE004693_4 (AE004693) NADH dehydrogenase I chain J [Pseudomonas aeruginosa PAO1]
Identities = 166/166 (100%), Positives = 166/166 (100%)
Sequence:
GTGGAATTCGCTTTCTATTTCGCTGCCGGCGTCGCCGTACTGGCCACGCTGCGGGTCATCACCAACAGCAAC
CCGGTGCATGCCCTGCTCTACCTGATCATTTCCCTGCTGGCCATCTCGATGACCTTCTTCAGCCTCGGCGCA
CCCTTCGCCGGCGCCCTGGAGATCATCGTCTACGCTGGCGCCATCATGGTTCTGTTCGTCTTCGTGGTGATG
ATGCTCAACCTCGGCCCGGCCGTGGTCGAGCAGGAGCGCAAGTGGCTCACGCCCGGCATCTGGGTCGGGCCT
TCGGCCCTGGCCCTGGTCCTGCTGGTGGAACTGCTGGTGGTCCTCGCCCGCACCCCGAGCGGTGCCGGCATC
GGCCATACCACGGTAGACGCCAAGGCGGTCGGCATCAGCCTCTACGGCCCGTACCTGCTGGTGGTCGAACTG
GCCTCCATGCTGTTGCTCGCGGCCCTGGTCGCTGCCTATCACCTGGGTCGTCAAGACGCCAAACAATAA
Translation:
MEFAFYFAAGVAVLATLRVITNSNPVHALLYLIISLLAISMTFFSLGAPFAGALEIIVYAGAIMVLFVFVVM
MLNLGPAVVEQERKWLTPGIWVGPSALALVLLVELLVVLARTPSGAGIGHTTVDAKAVGISLYGPYLLVVEL
ASMLLLAALVAAYHLGRQDAKQ*
AnnotationID:4413GeneID:4407
AnnotatorUID: diggins
Modification Date/Time: 2005-04-29 11:11:37
Gene Name:nuoJ
Confidence Code:2
GeneProduct:NADH dehydrogenase I chain J
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0839
Secondary Functional Category(ies):
EC Number:1.6.5.3
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO56851.1| (AE016868) NADH dehydrogenase I, J subunit [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 169

 Score =  263 bits (672), Expect = 3e-70
 Identities = 130/166 (78%), Positives = 148/166 (89%)

>gb|AAN69710.1|AE016607_4 (AE016789) NADH dehydrogenase I, J subunit [Pseudomonas putida
           KT2440]
          Length = 166

 Score =  247 bits (630), Expect = 2e-65
 Identities = 125/166 (75%), Positives = 142/166 (85%)

>gb|AAN81275.1|AE016763_234 (AE016763) NADH dehydrogenase I chain J [Escherichia coli CFT073]
          Length = 184

 Score =  213 bits (541), Expect = 5e-55
 Identities = 109/163 (66%), Positives = 134/163 (82%), Gaps = 1/163 (0%)








 
Structural Features:
COG0839, NuoJ, NADH:ubiquinone oxidoreductase subunit 6 (chain J) 
[Energy production and conversion]
CD-Length = 166 residues,  97.6% aligned
Score = 95.3 bits (237), Expect = 3e-21
Identities = 67/164 (40%), Positives = 97/164 (59%), Gaps = 3/164 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:15080




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:25958




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33218




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4407_cdd.html


    		




 
URL:

















    ReferenceID:34580




  
Author/Investigator(s):
        Camacho Carvajal MM, Wijfjes AH, Mulders IH, Lugtenberg BJ, Bloemberg GV.





  
Title:
        Characterization of NADH dehydrogenases of Pseudomonas fluorescens WCS365 and their role in competitive root colonization.




  
PubMed:
        12118882


MedLine:
        




    
Source:
Mol Plant Microbe Interact. 2002 Jul;15(7):662-71.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12118882

















    ReferenceID:34590




  
Author/Investigator(s):
        Dupuis A, Peinnequin A, Chevallet M, Lunardi J, Darrouzet E, Pierrard B, Procaccio V, Issartel JP.





  
Title:
        Identification of five Rhodobacter capsulatus genes encoding the equivalent of ND subunits of the mitochondrial NADH-ubiquinone oxidoreductase.




  
PubMed:
        8566820


MedLine:
        




    
Source:
Gene. 1995 Dec 29;167(1-2):99-104.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8566820









           
Homologs By Global Alignment 

           

       



     
Gene ID:4407
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6744
                           PA2645_tr
                           translation of PA2645
                           166
                           99.99
                           99.99
                           0
                           809.0
     

     
     
180872
                           gb|AAG06033.1|AE004693_4
                           (AE004693) NADH dehydrogenase I chain J [Pseudomonas aeruginosa PAO1]
                           166
                           99.39
                           99.99
                           0
                           806.0
     

     
     
180874
                           gb|AAL89572.1|
                           (AF281148) NADH dehydrogenase I subunit J [Pseudomonas fluorescens]
                           166
                           77.10
                           88.55
                           0
                           655.0
     

     
     
180873
                           gb|AAO56851.1|
                           (AE016868) NADH dehydrogenase I, J subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           169
                           76.33
                           87.57
                           3
                           668.0
     

     
     
180875
                           gb|AAN69710.1|AE016607_4
                           (AE016789) NADH dehydrogenase I, J subunit [Pseudomonas putida KT2440]
                           166
                           74.69
                           85.54
                           0
                           626.0
     

     






      

          
GC ORFID: 47409How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2645Glimmer Score: 

          
Start: 2364023Stop: 2364523

          
Length: 501


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA2645 translation of PA2645Homolog Bit Score 316.0

          
Other Homologs:  psyr_15may02_Scaffold17_revised_gene2773 (ORF: BLASTX 263.0), t_PA2645 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGGAATTCGCTTTCTATTTCGCTGCCGGCGTCGCCGTACTGGCCACGCTGCGGGTCATCACCAACAGCAAC 
CCGGTGCATGCCCTGCTCTACCTGATCATTTCCCTGCTGGCCATCTCGATGACCTTCTTCAGCCTCGGCGCA 
CCCTTCGCCGGCGCCCTGGAGATCATCGTCTACGCTGGCGCCATCATGGTTCTGTTCGTCTTCGTGGTGATG 
ATGCTCAACCTCGGCCCGGCCGTGGTCGAGCAGGAGCGCAAGTGGCTCACGCCCGGCATCTGGGTCGGGCCT 
TCGGCCCTGGCCCTGGTCCTGCTGGTGGAACTGCTGGTGGTCCTCGCCCGCACCCCGAGCGGTGCCGGCATC 
GGCCATACCACGGTAGACGCCAAGGCGGTCGGCATCAGCCTCTACGGCCCGTACCTGCTGGTGGTCGAACTG 
GCCTCCATGCTGTTGCTCGCGGCCCTGGTCGCTGCCTATCACCTGGGTCGTCAAGACGCCAAACAATAA