ViewGene
Locus Name:
GeneID:
Location:

GeneID:4404
TrivialName: PA14_45330
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_45330
PAO1 Ortholog Locus:PA1479
Sequence Length:489
Protein Length:162
Start: 4040216
Stop: 4039728
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04868.1|AE004577_3 (AE004577) cytochrome C-type biogenesis protein CcmE [Pseudomonas aeruginosa PAO1]
Identities = 161/162 (99%), Positives = 161/162 (99%)
Sequence:
GTGAATCCGGTCCGCAAGAAACGTCTGATCATCGTCCTCGCCATCGTGGTGGGCGTCGGCGCCGCGGTGGGC
CTGGCGCTATCGGCCctGcaacAGAACATCAACCTGTTCTACACCCCGACCCAGATCGCCAACGGCGAAGCG
CCGACCGACACCCGCATACGCGCCGGCGGCCTGGTGGAAAAGGGTTCTCTGCAACGCAGCGAGGATTCGCTG
AATGTGCGTTTCGTGGTCACCGACGGCGCCAAGGAAGTGACCATCGCCTACCATGGCATCCTTCCCGACCTG
TTCCGCGAGGGGCAGGGCATCGTCGCCCTCGGCAAGCTGGGCGGCGACGGCGTGCTGGTGGCCGATGAAGTG
CTGGCCAAGCACGACGAGAACTACATGCCGCCGGAAGTCACCAAGGCGCTCAAGGACAGCGGCCAACTGAAG
CACTACGAGAACGGCAAGGCCGCCGGCGAGACCTCGTACAACCAGGAGGGCAAGTAG
Translation:
MNPVRKKRLIIVLAIVVGVGAAVGLALSALQQNINLFYTPTQIANGEAPTDTRIRAGGLVEKGSLQRSEDSL
NVRFVVTDGAKEVTIAYHGILPDLFREGQGIVALGKLGGDGVLVADEVLAKHDENYMPPEVTKALKDSGQLK
HYENGKAAGETSYNQEGK*
AnnotationID:4410GeneID:4404
AnnotatorUID: diggins
Modification Date/Time: 2005-02-24 17:05:28
Gene Name:ccmE
Confidence Code:2
GeneProduct:cytochrome C-type biogenesis protein CcmE
Cell Localization:()
Synonyms:cycJ
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG2332,pfam03100
Secondary Functional Category(ies):posttranslational modification, protein turnover, chaperones
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69902.1|AE016629_4 (AE016790) cytochrome c-type biogenesis protein CcmE [Pseudomonas
           putida KT2440]
          Length = 151

 Score =  233 bits (593), Expect = 4e-61
 Identities = 120/155 (77%), Positives = 131/155 (84%), Gaps = 6/155 (3%)

>gb|AAC44225.1| (U44827) CycJ [Pseudomonas fluorescens]
          Length = 214

 Score =  227 bits (579), Expect = 2e-59
 Identities = 114/142 (80%), Positives = 128/142 (90%)

>gb|AAN81188.1|AE016763_147 (AE016763) Cytochrome c-type biogenesis protein ccmE [Escherichia
           coli CFT073]
          Length = 159

 Score =  136 bits (343), Expect = 4e-32
 Identities = 72/144 (50%), Positives = 93/144 (64%), Gaps = 6/144 (4%)






 
Structural Features:
COG2332, CcmE, Cytochrome c-type biogenesis protein CcmE 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 153 residues, 100.0% aligned
Score =  200 bits (509), Expect = 8e-53
Identities = 92/157 (58%), Positives = 111/157 (70%), Gaps = 4/157 (2%)

pfam03100, CcmE, CcmE. 
CD-Length = 131 residues, 100.0% aligned
Score =  176 bits (447), Expect = 1e-45
Identities = 80/132 (60%), Positives = 97/132 (73%), Gaps = 2/132 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:15076




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:25952




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37374




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4404_cdd.html


    		




 
URL:

















    ReferenceID:37951




  
Author/Investigator(s):
        Tanapongpipat S, Reid E, Cole JA, Crooke H.





  
Title:
        Transcriptional control and essential roles of the Escherichia coli ccm gene products in formate-dependent nitrite reduction and cytochrome c synthesis.




  
PubMed:
        9716493


MedLine:
        




    
Source:
Biochem J. 1998 Sep 1;334 ( Pt 2):355-65.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9716493

















    ReferenceID:37955




  
Author/Investigator(s):
        Thony-Meyer L, Fischer F, Kunzler P, Ritz D, Hennecke H.





  
Title:
        Escherichia coli genes required for cytochrome c maturation.




  
PubMed:
        7635817


MedLine:
        




    
Source:
J Bacteriol. 1995 Aug;177(15):4321-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7635817









           
Homologs By Global Alignment 

           

       



     
Gene ID:4404
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6741
                           PA1479_tr
                           translation of PA1479
                           162
                           99.38
                           99.38
                           0
                           810.0
     

     
     
180812
                           gb|AAG04868.1|AE004577_3
                           (AE004577) cytochrome C-type biogenesis protein CcmE [Pseudomonas aeruginosa PAO1]
                           162
                           98.76
                           99.38
                           0
                           807.0
     

     
     
180813
                           emb|CAA88017.1|
                           (Z47979) inner membrane or periplasmic protein [Pseudomonas fluorescens]
                           162
                           73.45
                           82.09
                           11
                           583.0
     

     
     
180814
                           gb|AAN69902.1|AE016629_4
                           (AE016790) cytochrome c-type biogenesis protein CcmE [Pseudomonas putida KT2440]
                           162
                           73.45
                           80.86
                           11
                           590.0
     

     
     
180816
                           gb|AAO57102.1|
                           (AE016869) cytochrome c-type biogenesis protein CcmE [Pseudomonas syringae pv. tomato str. DC3000]
                           162
                           68.51
                           80.24
                           5
                           551.0
     

     






      

          
GC ORFID: 47406How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1479Glimmer Score: 

          
Start: 913693Stop: 914181

          
Length: 489


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA1479 translation of PA1479Homolog Bit Score 316.0

          
Other Homologs:  psyr_15may02_Scaffold13_revised_gene2229 (ORF: BLASTX 229.0), t_PA1479 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGAATCCGGTCCGCAAGAAACGTCTGATCATCGTCCTCGCCATCGTGGTGGGCGTCGGCGCCGCGGTGGGC 
CTGGCGCTATCGGCCctGcaacAGAACATCAACCTGTTCTACACCCCGACCCAGATCGCCAACGGCGAAGCG 
CCGACCGACACCCGCATACGCGCCGGCGGCCTGGTGGAAAAGGGTTCTCTGCAACGCAGCGAGGATTCGCTG 
AATGTGCGTTTCGTGGTCACCGACGGCGCCAAGGAAGTGACCATCGCCTACCATGGCATCCTTCCCGACCTG 
TTCCGCGAGGGGCAGGGCATCGTCGCCCTCGGCAAGCTGGGCGGCGACGGCGTGCTGGTGGCCGATGAAGTG 
CTGGCCAAGCACGACGAGAACTACATGCCGCCGGAAGTCACCAAGGCGCTCAAGGACAGCGGCCAACTGAAG 
CACTACGAGAACGGCAAGGCCGCCGGCGAGACCTCGTACAACCAGGAGGGCAAGTAG