ViewGene
Locus Name:
GeneID:
Location:

GeneID:44
TrivialName: PA14_36730
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_36730
PAO1 Ortholog Locus:PA2152
Sequence Length:3303
Protein Length:1100
Start: 3275283
Stop: 3271981
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05540.1|AE004642_7 (AE004642) probable trehalose synthase [Pseudomonas aeruginosa PAO1]
Identities = 1096/1100 (99%), Positives = 1097/1100 (99%)
Sequence:
ATGGCCAGACGCGAGAAACCGGTCTTTCTCAACGACCCGCAATGGTACAAGGACGCGGTGATCTACCAGGTC
CACGTGAAATCCTTCTACGACGCCAACAACGATGGCATCGGCGACTTCGCCGGGCTGATCGAGAAGCTCGAC
TACATCGCCGACCTCGGCGTGAACACTCTCTGGCTGCTGCCGTTCTACCCGTCGCCACGCCGCGACGACGGC
TACGACATCGCCCAGTACCGTGGCGTGCACAGCGACTATGGCAGCCTCGCCGACGCCCGCCGGTTCATCGCC
GAGGCCCATCGACGCGGTCTGCGGGTGATTACCGAGCTGGTGATCAACCACACCTCCGACCAGCATCCCTGG
TTCATCCGCGCCCGCCACGCGAAGAAGGGGTCGCGCGCCCGCGACTACTACGTCTGGTCGGACAGCGACGAG
AAATACCAGGGTACGCGGATCATCTTCATCGACACCGAGCAGTCCAACTGGACCTGGGACCCGGTAGCCCAA
CAGTACTACTGGCACCGCTTCTATTCCCACCAGCCGGACCTGAACTTCGACAACCCGCAGGTCCTGCGCGAG
GTCCTCGGGGTGATGCGCTACTGGCTGGACATGGGCGTCGACGGCCTGCGCCTGGACGCGATTCCCTACCTG
ATCGAACGCGACGGCACCAGCAGCGAGAACCTGCCGGAGACCCACCAGGTGCTCAAGCGCATCCGCGCCGAG
CTGGACGCGCACTATCCCGACCGCATGCTGCTGGCCGAGGCCAACCAGTGGCCGGAAGACACCCGGCCGTAC
TTCGGCGGCGAGGATGGCGGCGAGGGCGACGAATGCCACATGGCCTTCCACTTCCCGCTGATGCCGCGCATG
TACATGGCCATCGCCCAGGAGGATCGCTATCCGATCACCGACATCCTGCGCCAGACCCCGGACATCCCGGCC
AATTGCCAATGGGCGATCTTCCTGCGCAACCACGACGAGCTGACCCTGGAGATGGTCACCGACGACGAGCGC
GACTATCTCTGGAACCACTATGCCGCCGACCGCCGCGCGCGCCTCAACCTGGGCATCCGCCGGCGCCTGGCG
CCGCTGGTGGAGCGTGACCGGCGGCGCATCGAGCTGCTGCACAGCCTGCTGTTGTCGATGCCGGGCACGCCG
ACCCTGTACTACGGCGACGAGATCGGCATGGGCGACAACATCTACCTCGGCGACCGCGACGGCGTGCGGACC
CCGATGCAGTGGTCGGTGGACCGCAACGGCGGCTTCTCCCGCGCCGACCCGGCGAAGCTGGTGCTGCCGCCG
ATCCTCGACCCGCTGTACGGCTACCAGACGATCAACGTCGAGGCGCAGGCGCGCGACCCGCATTCGCTGCTC
AACTGGATGCGCCGCCTGCTCGCCGTGCGCAGCCAGCAGAAGGCCTTCGGCCGCGGCAGCCTGAAGATGCTC
GCGCCGAGCAACCGACGGATCCTCGCCTACCTGCGCGAATACGCCGAGGGCGAACGGCAGGACAGCATCCTC
TGCGTGGCCAACCTGTCGCGCGCGGCCCAGGCGGTGGAACTGGACCTCGCCAGCCATGCCGGCAAGGTGCCG
GTGGAGATGATCGGCGGCATGTCGTTCCCGCCGATCGGCGAGCTGACCTACCTGCTGACCCTGCCGCCCTAC
GGCTTCTACTGGTTCTACCTGGCCGACGCGACGCAGATGCCGAGCTGGCACGTGGCCGCCGACGAGCGCCTG
CCGGAACTGCCGACCCTGGTGGTCAAGCAGCGCCTCGGCGAGCTGCTGCAGGGCGCATCGCGGAACATCCTG
GAAGGCGAGACACTGCCGGCCTACCTGCCGAAGCGCCGCTGGTTCGCCGGCGAGAAAGGCCAGCCACGGCTC
TGCTACATCGTTCCGCTGGACGAGGCGGAGCCGCGCTGTGCGCTGTGCGAAGTGGAGATCGACGGGCTGCGC
TACCAGTTGCCGCTGGGCTTCCTCGACGCCGACCAGCGCGGCGACAGCCTGCCTCAGTTGCTGGCCCTGGCG
CGCCTGCGGCGCGGGCGCAAGGTCGGCCTGCTGACCGACGCCGCCAGCCTGCCGCTGTTCGCCCGCAAGGTC
CTCGCGCAACTCCGCGCCGAGGCGGTGATCGCCCACGGCGACGGCGAGATCCAGTTCATCCCGGCGGCGGGA
CTGGCGGAACTGGACGACATCGCCGACGAAGACGTCCTGCCGTTCTCGGTGGAACAGTCCAACAGCTCGATC
CGCTTCGGCGAGCGCATGGTCCTCAAGGTGCTGCGCAAGATCCTTCCGGGGCTGCACCCGGAAATCGAGGTC
GGCGGCTACCTGACCCGCCACGGCTACCCCGGCATCGCCCCGTTGCTCGGCGAGGTACGGCGGGTCGGCGCG
GATGGCGAGCCGCACACGCTGATGATCCTGCAGGGCTACCTGAACAACCAGGGCGACGCCTGGAACTGGACC
CTGGACAACCTGGAGCGAGCCGTGCGCGACGAGATATCCGCGGCCAGCGAGGCGCTCGAAGGGCAATACGAC
AGCCTCGCCGAACTGCGCGGCTTCGCCGCCAACCTCGGCGCCCGGCTCGGCGAGATGCACAGCGTACTGGCC
GGCGAGTCGGACGATCCGGCCTTCGGCAGCCGCGAGAGCGACGAAGCCAGCGTCCAGGCCTGGGCGCTGCGC
ATCGCCGAACAGTTGCGCGAAGCCGGCAAACGCCTGAGCGAGCCGCCACGGCCGTTGCAGGGCGAGGCGGCG
GAACAGGCGCGGCGCCTGCTGGAACGCCTGCCGGCGCTGCTCGAACGCCTGCCGCTGCTGGCCCGGCAAGCA
GCCGGCGGGCTGCTGATCAGGGTTCACGGCGACCTGCACCTGGGCCAGGTGCTGATGGTCCAGGGCGACGCA
CGCTTCATCGACTTCGAGGGCGAGCCGGCGCGCAGCCTGGAAGAGCGCCGCCAGCGCCACAGTCCGATGAAG
GACGTCGGCGGCATGCTGCGCTCGTTCGACTACGCGGCGGCGATGGTCCTGCGCAACGCGCAGAGCACCGAT
AGCTCGGAACAGGCCGACAGCGCCCGGCGCAAGGTCGCCATGCGCTATCGCAGCGAAGCCCGCGACGCCTTC
CTGGTCGGCTACCGCGCCGCCGCAGCGGGCCTCGCGCACGCCTGGCACGGCCGCGAAGGCGAGGGCGCGGCG
CTGGTCCTGGCCTGCCTGGAAAAGGCCGCCTACGAACTGCTCTACGAAGCCGACTACCGCCCCGACTGGCTG
GAAGTGCCTCTCGCCGGTCTGGCCGAACTCACCGAACACCTCCTGAAGGGCAAGAACCGATGA
Translation:
MARREKPVFLNDPQWYKDAVIYQVHVKSFYDANNDGIGDFAGLIEKLDYIADLGVNTLWLLPFYPSPRRDDG
YDIAQYRGVHSDYGSLADARRFIAEAHRRGLRVITELVINHTSDQHPWFIRARHAKKGSRARDYYVWSDSDE
KYQGTRIIFIDTEQSNWTWDPVAQQYYWHRFYSHQPDLNFDNPQVLREVLGVMRYWLDMGVDGLRLDAIPYL
IERDGTSSENLPETHQVLKRIRAELDAHYPDRMLLAEANQWPEDTRPYFGGEDGGEGDECHMAFHFPLMPRM
YMAIAQEDRYPITDILRQTPDIPANCQWAIFLRNHDELTLEMVTDDERDYLWNHYAADRRARLNLGIRRRLA
PLVERDRRRIELLHSLLLSMPGTPTLYYGDEIGMGDNIYLGDRDGVRTPMQWSVDRNGGFSRADPAKLVLPP
ILDPLYGYQTINVEAQARDPHSLLNWMRRLLAVRSQQKAFGRGSLKMLAPSNRRILAYLREYAEGERQDSIL
CVANLSRAAQAVELDLASHAGKVPVEMIGGMSFPPIGELTYLLTLPPYGFYWFYLADATQMPSWHVAADERL
PELPTLVVKQRLGELLQGASRNILEGETLPAYLPKRRWFAGEKGQPRLCYIVPLDEAEPRCALCEVEIDGLR
YQLPLGFLDADQRGDSLPQLLALARLRRGRKVGLLTDAASLPLFARKVLAQLRAEAVIAHGDGEIQFIPAAG
LAELDDIADEDVLPFSVEQSNSSIRFGERMVLKVLRKILPGLHPEIEVGGYLTRHGYPGIAPLLGEVRRVGA
DGEPHTLMILQGYLNNQGDAWNWTLDNLERAVRDEISAASEALEGQYDSLAELRGFAANLGARLGEMHSVLA
GESDDPAFGSRESDEASVQAWALRIAEQLREAGKRLSEPPRPLQGEAAEQARRLLERLPALLERLPLLARQA
AGGLLIRVHGDLHLGQVLMVQGDARFIDFEGEPARSLEERRQRHSPMKDVGGMLRSFDYAAAMVLRNAQSTD
SSEQADSARRKVAMRYRSEARDAFLVGYRAAAAGLAHAWHGREGEGAALVLACLEKAAYELLYEADYRPDWL
EVPLAGLAELTEHLLKGKNR*
AnnotationID:54GeneID:44
AnnotatorUID: diggins
Modification Date/Time: 2005-03-03 12:12:15
Gene Name:
Confidence Code:3
GeneProduct:putative trehalose synthase
Cell Localization:()
Synonyms:PA2152
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:putative glycosidase
Functional Category Confidence Code:5
COGs:COG3281,COG0366,smart00642,pfam00128
Secondary Functional Category(ies):putative enzyme
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO56261.1| (AE016865) alpha-amylase family protein [Pseudomonas syringae pv.
            tomato str. DC3000]
          Length = 1108

 Score = 1483 bits (3839), Expect = 0.0
 Identities = 730/1102 (66%), Positives = 877/1102 (79%), Gaps = 11/1102 (0%)

>gb|AAN69649.1|AE016599_4 (AE016789) trehalose synthase, putative [Pseudomonas putida KT2440]
          Length = 1106

 Score = 1481 bits (3834), Expect = 0.0
 Identities = 732/1098 (66%), Positives = 865/1098 (78%), Gaps = 14/1098 (1%)

>gb|AAM39453.1| (AE012109) trehalose synthase [Xanthomonas campestris pv. campestris
            str. ATCC 33913]
          Length = 1116

 Score = 1207 bits (3124), Expect = 0.0
 Identities = 627/1119 (56%), Positives = 777/1119 (69%), Gaps = 38/1119 (3%)






 
Structural Features:
COG3281, Ble, Uncharacterized protein, probably involved in trehalose biosynthesis 
[Carbohydrate transport and metabolism]
CD-Length = 438 residues,  99.1% aligned
Score =  493 bits (1269), Expect = 6e-140
Identities = 207/477 (43%), Positives = 252/477 (52%), Gaps = 43/477 (9%)

COG0366, AmyA, Glycosidases [Carbohydrate transport and metabolism]
CD-Length = 505 residues, 100.0% aligned
Score =  352 bits (903), Expect = 2e-97
Identities = 188/544 (34%), Positives = 258/544 (47%), Gaps = 70/544 (12%)

smart00642, Aamy, Alpha-amylase domain
CD-Length = 367 residues,  93.7% aligned
Score =  272 bits (696), Expect = 2e-73
Identities = 131/396 (33%), Positives = 189/396 (47%), Gaps = 58/396 (14%)

pfam00128, Alpha-amylase, Alpha amylase, catalytic domain.
CD-Length = 357 residues,  98.6% aligned
Score =  211 bits (537), Expect = 5e-55
Identities = 120/411 (29%), Positives = 177/411 (43%), Gaps = 67/411 (16%)








   
Genomic Context:






   
Comment:


















    ReferenceID:91




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17233




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32576




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:44_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:44
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
85
                           PA2152_tr
                           translation of PA2152
                           1100
                           99.63
                           99.72
                           0
                           5759.0
     

     
     
11633
                           gb|AAG05540.1|AE004642_7
                           (AE004642) probable trehalose synthase [Pseudomonas aeruginosa PAO1]
                           1100
                           99.63
                           99.72
                           0
                           5759.0
     

     
     
11635
                           gb|AAN69649.1|AE016599_4
                           (AE016789) trehalose synthase, putative [Pseudomonas putida KT2440]
                           1120
                           65.80
                           77.85
                           34
                           3847.0
     

     
     
11634
                           gb|AAO56261.1|
                           (AE016865) alpha-amylase family protein [Pseudomonas syringae pv. tomato str. DC3000]
                           1115
                           65.56
                           78.74
                           22
                           3844.0
     

     
     
11639
                           emb|CAE33355.1|
                           (BX640445) alpha-glucosidase [Bordetella bronchiseptica] [Bordetella bronchiseptica RB50]
                           1142
                           56.91
                           67.25
                           71
                           3156.0
     

     






      

          
GC ORFID: 43046How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2152Glimmer Score: 

          
Start: 1678631Stop: 1681933

          
Length: 3303


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2152 translation of PA2152Homolog Bit Score 2222.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1410 (ORF: BLASTX 1482.0), t_PA2152 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCCAGACGCGAGAAACCGGTCTTTCTCAACGACCCGCAATGGTACAAGGACGCGGTGATCTACCAGGTC 
CACGTGAAATCCTTCTACGACGCCAACAACGATGGCATCGGCGACTTCGCCGGGCTGATCGAGAAGCTCGAC 
TACATCGCCGACCTCGGCGTGAACACTCTCTGGCTGCTGCCGTTCTACCCGTCGCCACGCCGCGACGACGGC 
TACGACATCGCCCAGTACCGTGGCGTGCACAGCGACTATGGCAGCCTCGCCGACGCCCGCCGGTTCATCGCC 
GAGGCCCATCGACGCGGTCTGCGGGTGATTACCGAGCTGGTGATCAACCACACCTCCGACCAGCATCCCTGG 
TTCATCCGCGCCCGCCACGCGAAGAAGGGGTCGCGCGCCCGCGACTACTACGTCTGGTCGGACAGCGACGAG 
AAATACCAGGGTACGCGGATCATCTTCATCGACACCGAGCAGTCCAACTGGACCTGGGACCCGGTAGCCCAA 
CAGTACTACTGGCACCGCTTCTATTCCCACCAGCCGGACCTGAACTTCGACAACCCGCAGGTCCTGCGCGAG 
GTCCTCGGGGTGATGCGCTACTGGCTGGACATGGGCGTCGACGGCCTGCGCCTGGACGCGATTCCCTACCTG 
ATCGAACGCGACGGCACCAGCAGCGAGAACCTGCCGGAGACCCACCAGGTGCTCAAGCGCATCCGCGCCGAG 
CTGGACGCGCACTATCCCGACCGCATGCTGCTGGCCGAGGCCAACCAGTGGCCGGAAGACACCCGGCCGTAC 
TTCGGCGGCGAGGATGGCGGCGAGGGCGACGAATGCCACATGGCCTTCCACTTCCCGCTGATGCCGCGCATG 
TACATGGCCATCGCCCAGGAGGATCGCTATCCGATCACCGACATCCTGCGCCAGACCCCGGACATCCCGGCC 
AATTGCCAATGGGCGATCTTCCTGCGCAACCACGACGAGCTGACCCTGGAGATGGTCACCGACGACGAGCGC 
GACTATCTCTGGAACCACTATGCCGCCGACCGCCGCGCGCGCCTCAACCTGGGCATCCGCCGGCGCCTGGCG 
CCGCTGGTGGAGCGTGACCGGCGGCGCATCGAGCTGCTGCACAGCCTGCTGTTGTCGATGCCGGGCACGCCG 
ACCCTGTACTACGGCGACGAGATCGGCATGGGCGACAACATCTACCTCGGCGACCGCGACGGCGTGCGGACC 
CCGATGCAGTGGTCGGTGGACCGCAACGGCGGCTTCTCCCGCGCCGACCCGGCGAAGCTGGTGCTGCCGCCG 
ATCCTCGACCCGCTGTACGGCTACCAGACGATCAACGTCGAGGCGCAGGCGCGCGACCCGCATTCGCTGCTC 
AACTGGATGCGCCGCCTGCTCGCCGTGCGCAGCCAGCAGAAGGCCTTCGGCCGCGGCAGCCTGAAGATGCTC 
GCGCCGAGCAACCGACGGATCCTCGCCTACCTGCGCGAATACGCCGAGGGCGAACGGCAGGACAGCATCCTC 
TGCGTGGCCAACCTGTCGCGCGCGGCCCAGGCGGTGGAACTGGACCTCGCCAGCCATGCCGGCAAGGTGCCG 
GTGGAGATGATCGGCGGCATGTCGTTCCCGCCGATCGGCGAGCTGACCTACCTGCTGACCCTGCCGCCCTAC 
GGCTTCTACTGGTTCTACCTGGCCGACGCGACGCAGATGCCGAGCTGGCACGTGGCCGCCGACGAGCGCCTG 
CCGGAACTGCCGACCCTGGTGGTCAAGCAGCGCCTCGGCGAGCTGCTGCAGGGCGCATCGCGGAACATCCTG 
GAAGGCGAGACACTGCCGGCCTACCTGCCGAAGCGCCGCTGGTTCGCCGGCGAGAAAGGCCAGCCACGGCTC 
TGCTACATCGTTCCGCTGGACGAGGCGGAGCCGCGCTGTGCGCTGTGCGAAGTGGAGATCGACGGGCTGCGC 
TACCAGTTGCCGCTGGGCTTCCTCGACGCCGACCAGCGCGGCGACAGCCTGCCTCAGTTGCTGGCCCTGGCG 
CGCCTGCGGCGCGGGCGCAAGGTCGGCCTGCTGACCGACGCCGCCAGCCTGCCGCTGTTCGCCCGCAAGGTC 
CTCGCGCAACTCCGCGCCGAGGCGGTGATCGCCCACGGCGACGGCGAGATCCAGTTCATCCCGGCGGCGGGA 
CTGGCGGAACTGGACGACATCGCCGACGAAGACGTCCTGCCGTTCTCGGTGGAACAGTCCAACAGCTCGATC 
CGCTTCGGCGAGCGCATGGTCCTCAAGGTGCTGCGCAAGATCCTTCCGGGGCTGCACCCGGAAATCGAGGTC 
GGCGGCTACCTGACCCGCCACGGCTACCCCGGCATCGCCCCGTTGCTCGGCGAGGTACGGCGGGTCGGCGCG 
GATGGCGAGCCGCACACGCTGATGATCCTGCAGGGCTACCTGAACAACCAGGGCGACGCCTGGAACTGGACC 
CTGGACAACCTGGAGCGAGCCGTGCGCGACGAGATATCCGCGGCCAGCGAGGCGCTCGAAGGGCAATACGAC 
AGCCTCGCCGAACTGCGCGGCTTCGCCGCCAACCTCGGCGCCCGGCTCGGCGAGATGCACAGCGTACTGGCC 
GGCGAGTCGGACGATCCGGCCTTCGGCAGCCGCGAGAGCGACGAAGCCAGCGTCCAGGCCTGGGCGCTGCGC 
ATCGCCGAACAGTTGCGCGAAGCCGGCAAACGCCTGAGCGAGCCGCCACGGCCGTTGCAGGGCGAGGCGGCG 
GAACAGGCGCGGCGCCTGCTGGAACGCCTGCCGGCGCTGCTCGAACGCCTGCCGCTGCTGGCCCGGCAAGCA 
GCCGGCGGGCTGCTGATCAGGGTTCACGGCGACCTGCACCTGGGCCAGGTGCTGATGGTCCAGGGCGACGCA 
CGCTTCATCGACTTCGAGGGCGAGCCGGCGCGCAGCCTGGAAGAGCGCCGCCAGCGCCACAGTCCGATGAAG 
GACGTCGGCGGCATGCTGCGCTCGTTCGACTACGCGGCGGCGATGGTCCTGCGCAACGCGCAGAGCACCGAT 
AGCTCGGAACAGGCCGACAGCGCCCGGCGCAAGGTCGCCATGCGCTATCGCAGCGAAGCCCGCGACGCCTTC 
CTGGTCGGCTACCGCGCCGCCGCAGCGGGCCTCGCGCACGCCTGGCACGGCCGCGAAGGCGAGGGCGCGGCG 
CTGGTCCTGGCCTGCCTGGAAAAGGCCGCCTACGAACTGCTCTACGAAGCCGACTACCGCCCCGACTGGCTG 
GAAGTGCCTCTCGCCGGTCTGGCCGAACTCACCGAACACCTCCTGAAGGGCAAGAACCGATGA