ViewGene
Locus Name:
GeneID:
Location:

GeneID:4374
TrivialName: PA14_62570
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_62570
PAO1 Ortholog Locus:PA4728
Sequence Length:489
Protein Length:162
Start: 5582656
Stop: 5583144
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08114.1|AE004886_6 (AE004886) 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase [Pseudomonas aeruginosa PAO1]
Identities = 160/162 (98%), Positives = 161/162 (99%)
Sequence:
ATGATCCAGCGCGTCTACGTGGCGCTGGGCAGCAACCTGGCCGAACCGCGCGAGCAGATCCAGGCTGCCCTC
GATGCCTTCGAGCGGCTTCCCGAGACCCGCCTGGTCGCCGTCTCGCCGCTCTATGTCAGCGACCCGCTCGGC
CCCGCCGACCAGCCGCGCTTCGTCAACGGCGTGGCGGCCCTCGACACCACCCTGGCGCCGCTCGACCTGCTC
GACGCCCTGCAGGCCATCGAACTGGAACAGGGGCGCGTCCGCGACCTGCGCTGGGGTCCGCGCACGCTCGAC
CTGGACATCCTGCTGTTCGGCGAACAATTGCTCGACCTGCCGCGCCTGAAGGTGCCGCACTACCACATGCAG
GCGCGCGCTTTCGTGCTCTATCCCCTCGCCGATCTCGCCCCCGACCTGCGCCTGCCCGATGGCCGCCACCTG
CCGGAGCTGCTCGCGGCCTGTCCGTTCGAGGGCATCGAACGCCTTCCGGGCGCCTGA
Translation:
MIQRVYVALGSNLAEPREQIQAALDAFERLPETRLVAVSPLYVSDPLGPADQPRFVNGVAALDTTLAPLDLL
DALQAIELEQGRVRDLRWGPRTLDLDILLFGEQLLDLPRLKVPHYHMQARAFVLYPLADLAPDLRLPDGRHL
PELLAACPFEGIERLPGA*
AnnotationID:4380GeneID:4374
AnnotatorUID: diggins
Modification Date/Time: 2005-04-27 06:06:08
Gene Name:folK
Confidence Code:2
GeneProduct:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase
Functional Category Confidence Code:5
COGs:COG0801,cd00483,pfam01288
Secondary Functional Category(ies):coenzyme metabolism
EC Number:2.7.6.3
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO54496.1| (AE016859) 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
           pyrophosphokinase [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 168

 Score =  224 bits (571), Expect = 2e-58
 Identities = 112/160 (70%), Positives = 131/160 (81%), Gaps = 1/160 (0%)

>gb|AAO60159.1| (AY210414) 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase;
           FolK [Pseudomonas fluorescens]
          Length = 154

 Score =  212 bits (540), Expect = 6e-55
 Identities = 110/153 (71%), Positives = 126/153 (82%), Gaps = 2/153 (1%)

>gb|AAN78669.1|AE016755_169 (AE016755) 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
           pyrophosphokinase [Escherichia coli CFT073]
          Length = 159

 Score =  167 bits (424), Expect = 2e-41
 Identities = 84/154 (54%), Positives = 111/154 (72%), Gaps = 1/154 (0%)








 
Structural Features:
COG0801, FolK, 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase 
[Coenzyme metabolism]
CD-Length = 160 residues,  98.8% aligned
Score =  187 bits (476), Expect = 6e-49
Identities = 87/158 (55%), Positives = 114/158 (72%)

cd00483, HPPK, 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase (HPPK).
CD-Length = 128 residues, 100.0% aligned
Score =  169 bits (430), Expect = 1e-43

pfam01288, HPPK, 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase (HPPK).
CD-Length = 128 residues, 100.0% aligned
Score =  150 bits (380), Expect = 8e-38
Identities = 63/128 (49%), Positives = 90/128 (70%)








   
Genomic Context:






   
Comment:


















    ReferenceID:8749




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:25892




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37641




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4374_cdd.html


    		




 
URL:

















    ReferenceID:37740




  
Author/Investigator(s):
        Talarico TL, Ray PH, Dev IK, Merrill BM, Dallas WS.





  
Title:
        Cloning, sequence analysis, and overexpression of Escherichia coli folK, the gene coding for 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase.




  
PubMed:
        1325970


MedLine:
        




    
Source:
J Bacteriol. 1992 Sep;174(18):5971-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1325970









           
Homologs By Global Alignment 

           

       



     
Gene ID:4374
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6709
                           PA4728_tr
                           translation of PA4728
                           162
                           98.76
                           99.38
                           0
                           823.0
     

     
     
180187
                           gb|AAG08114.1|AE004886_6
                           (AE004886) 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase [Pseudomonas aeruginosa PAO1]
                           162
                           98.76
                           99.38
                           0
                           823.0
     

     
     
180189
                           gb|AAO60159.1|
                           (AY210414) 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; FolK [Pseudomonas fluorescens]
                           163
                           67.48
                           77.30
                           10
                           540.0
     

     
     
180190
                           gb|AAN70271.1|AE016667_4
                           (AE016791) 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase [Pseudomonas putida KT2440]
                           163
                           67.48
                           75.46
                           5
                           531.0
     

     
     
180188
                           gb|AAO54496.1|
                           (AE016859) 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase [Pseudomonas syringae pv. tomato str. DC3000]
                           169
                           66.27
                           77.51
                           8
                           571.0
     

     






      

          
GC ORFID: 47376How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4728Glimmer Score: 

          
Start: 4846336Stop: 4846824

          
Length: 489


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4728 translation of PA4728Homolog Bit Score 321.0

          
Other Homologs:  psyr_15may02_Scaffold15_revised_gene2519 (ORF: BLASTX 217.0), t_PA4728 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGATCCAGCGCGTCTACGTGGCGCTGGGCAGCAACCTGGCCGAACCGCGCGAGCAGATCCAGGCTGCCCTC 
GATGCCTTCGAGCGGCTTCCCGAGACCCGCCTGGTCGCCGTCTCGCCGCTCTATGTCAGCGACCCGCTCGGC 
CCCGCCGACCAGCCGCGCTTCGTCAACGGCGTGGCGGCCCTCGACACCACCCTGGCGCCGCTCGACCTGCTC 
GACGCCCTGCAGGCCATCGAACTGGAACAGGGGCGCGTCCGCGACCTGCGCTGGGGTCCGCGCACGCTCGAC 
CTGGACATCCTGCTGTTCGGCGAACAATTGCTCGACCTGCCGCGCCTGAAGGTGCCGCACTACCACATGCAG 
GCGCGCGCTTTCGTGCTCTATCCCCTCGCCGATCTCGCCCCCGACCTGCGCCTGCCCGATGGCCGCCACCTG 
CCGGAGCTGCTCGCGGCCTGTCCGTTCGAGGGCATCGAACGCCTTCCGGGCGCCTGA