ViewGene
Locus Name:
GeneID:
Location:

GeneID:434
TrivialName: PA14_10290
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_10290
PAO1 Ortholog Locus:PA4147
Sequence Length:1878
Protein Length:625
Start: 886477
Stop: 888354
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07534.1|AE004831_6 (AE004831) transcriptional regulator AcoR [Pseudomonas aeruginosa PAO1]
Identities = 623/625 (99%), Positives = 623/625 (99%)
Sequence:
ATGCTTTCCGCACACTCGAAGGACCATGTGGACTGTGTCAGCCGTGTCCTGAGGAACGCCGCGCGCCTGCCG
CAGGCGCCGGTGCCGACGCCGATCCTCGACTCCTGGCGCCGCTCCATGGAGCAGCACCGTCTCGACCCGGGG
TCCCTGCAAGGGCCGCGGATTCTCTCGCAGACCCTCCTCGACGAATGTCGCGAGCGTTCCGAACTGTTCCTG
CGCATCGCCAGCGAAGAGGTGGCGCGACTGCATGGACGGGTGCGCGATGCCGACTACTGCGTACTGCTCACC
GATGCCCAGGGGCGGACCATCGACTACCGTGTCGAGTCGACCATCCGCAACGACTGCCGCAAGGCCGGGCTG
TACCTCGGCACCTGCTGGTCGGAAGGCGAGGAGGGCACCTGCGGGGTGGCCGCCGTACTCACCGACAGGACT
GCGGTGACGGTGCACAAGCGCGATCACTTCCGTGCCGCCTTCATCGACCTGACCTGTTCCGCGGCGCCGGTC
TTCGATCCGCAGGGGGAGTTGCTCGGTGTGCTCGACGTTTCCGCCGTGCAATCGCCGGACGACCGCCGTAGC
CAGCATCTGATCCGGCAGATGGTGGCGCAGAGCGCGCGGGAGATCGAGAACGCCTTCTTCATGCACAGCGCC
CAGGGCCATTGGGTGCTGCGCGCGCACCGTGCGCCGGGCTACGTCGACAGCCAGCCGGACTACCTGTTCGCC
TGGGACGCCGATGGGCGCCTGCTGGCGTTGAACCCGGCGGCGCGGCACTACCTGCGGCAGCGCCTGGGGCAG
GTGCCGAGCCACATCGGCGAGGTGTTCGACCTCGACGCCCTGCACGCCGCCGCTGACCAGGCGCCGTGCCTG
CTGCGCGGCCAGGCCGGCGAGCTGCACGTGCGTCTCAGCGCGCCGCGGGCCAAGGCGCGGCGGCTGGAGCGC
GAGGTGCCCGACGACGCTGCGCTCGACCCGCGCATCGCCGAAAGCCTGCGCCTGGCGGTGCGGGTCAAGGAC
CGCAACCTGCCGGTGCTGATCCAGGGCGAGACCGGCGCCGGCAAGGAGGTTTTCGCCCGCCAGTTGCACCAG
GCCAGCGCCCGTCGCGACAAACCCTTCGTCGCGCTCAACTGCGCGGCGATTCCCGAAAGCCTGATCGAGAGC
GAGCTGTTCGGCTACGTTGGCGGAGCCTTTACCGGCGCGGCGGCGAAAGGGATGCGCGGCCTGCTGCAGCAG
GCCGATGGCGGTACCCTGTTCCTCGACGAGATCGGTGACATGCCGCTGGGCCTGCAGACCCGCCTGCTGCGC
GTGCTGGCCGAGGGCGAGGTGGCGCCGCTGGGGGCTGCGCGCCGGCAGGCTGTGGATATCCAGGTGATCTGC
GCCACCCACCGCGACCTGGCGGCGCTGGTGGCGGCCGGCGGCTTCCGCGAGGATCTCTACTTCCGCCTCGGC
GGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGAC
GAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGcAGCCcctgaATgcctgctCGGCTATCGC
tggccGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGcggcggcGCCACCCTG
CaACTCGCCGACCTTCCGGCAGAGCTGcgTGGCGaAGGCCGGACACCGGCCAGTGCGTGCGAGAGCGGCGGC
GGTCCGGAGCGCGACGCCCTGCTCGACGCCCTCGTGCGGCATCGCTGGAAACCCATGGCCGCGGCGCGGGAA
CTGGGTATCTCGCGAGCGACCCTGTATCGGCGGGTGCGCCGGCATGGCATCCGCATGCCGGGCCAGGCGGAC
GACTGA
Translation:
MLSAHSKDHVDCVSRVLRNAARLPQAPVPTPILDSWRRSMEQHRLDPGSLQGPRILSQTLLDECRERSELFL
RIASEEVARLHGRVRDADYCVLLTDAQGRTIDYRVESTIRNDCRKAGLYLGTCWSEGEEGTCGVAAVLTDRT
AVTVHKRDHFRAAFIDLTCSAAPVFDPQGELLGVLDVSAVQSPDDRRSQHLIRQMVAQSAREIENAFFMHSA
QGHWVLRAHRAPGYVDSQPDYLFAWDADGRLLALNPAARHYLRQRLGQVPSHIGEVFDLDALHAAADQAPCL
LRGQAGELHVRLSAPRAKARRLEREVPDDAALDPRIAESLRLAVRVKDRNLPVLIQGETGAGKEVFARQLHQ
ASARRDKPFVALNCAAIPESLIESELFGYVGGAFTGAAAKGMRGLLQQADGGTLFLDEIGDMPLGLQTRLLR
VLAEGEVAPLGAARRQAVDIQVICATHRDLAALVAAGGFREDLYFRLGGARFELPPLRERSDRLALIRRILD
EETAHCGVRIELGEAAPECLLGYRWPGNVRQLRHVLRYACALCGGATLQLADLPAELRGEGRTPASACESGG
GPERDALLDALVRHRWKPMAAARELGISRATLYRRVRRHGIRMPGQADD*
AnnotationID:443GeneID:434
AnnotatorUID: diggins
Modification Date/Time: 2005-03-30 15:03:10
Gene Name:acoR
Confidence Code:2
GeneProduct:transcriptional regulator AcoR
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(25) Transcriptional regulators
Alternate Gene Product Name:acetoin catabolism regulatory protein
Functional Category Confidence Code:5
COGs:COG3284,COG1221,pfam00158
Secondary Functional Category(ies):secondary metabolites, biosynthesis, transport, and catabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66184.1|AE016246_1 (AE016776) acetoin catabolism regulatory protein [Pseudomonas
           putida KT2440]
          Length = 617

 Score =  890 bits (2299), Expect = 0.0
 Identities = 460/621 (74%), Positives = 510/621 (82%), Gaps = 9/621 (1%)

>emb|CAD16838.1| (AL646073) PROBABLE TRANSCRIPTION REGULATOR PROTEIN [Ralstonia
           solanacearum]
          Length = 637

 Score =  467 bits (1202), Expect = e-131
 Identities = 279/608 (45%), Positives = 363/608 (59%), Gaps = 25/608 (4%)

>gb|AAM35543.1| (AE011694) transcriptional regulator acoR [Xanthomonas axonopodis
           pv. citri str. 306]
          Length = 661

 Score =  419 bits (1077), Expect = e-116
 Identities = 255/623 (40%), Positives = 346/623 (55%), Gaps = 34/623 (5%)






 
Structural Features:
COG3284, AcoR, Transcriptional activator of acetoin/glycerol metabolism 
[Secondary metabolites biosynthesis, transport, and catabolism / Transcription]
CD-Length = 606 residues, 100.0% aligned
Score =  692 bits (1788), Expect = 0.0
Identities = 314/622 (50%), Positives = 378/622 (60%), Gaps = 20/622 (3%)

COG1221, PspF, Transcriptional regulators containing an AAA-type 
ATPase domain and a DNA-binding domain 
[Transcription / Signal transduction mechanisms]
CD-Length = 403 residues,  87.1% aligned
Score =  239 bits (610), Expect = 1e-63
Identities = 143/400 (35%), Positives = 186/400 (46%), Gaps = 34/400 (8%)

pfam00158, Sigma54_activat, Sigma-54 interaction domain.
CD-Length = 222 residues,  98.2% aligned
Score =  247 bits (633), Expect = 2e-66
Identities = 114/219 (52%), Positives = 142/219 (64%), Gaps = 3/219 (1%)







   
Genomic Context:






   
Comment:


















    ReferenceID:871




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18013




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36880




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:434_cdd.html


    		




 
URL:

















    ReferenceID:36979




  
Author/Investigator(s):
        Kruger N, Steinbuchel A.





  
Title:
        Identification of acoR, a regulatory gene for the expression of genes essential for acetoin catabolism in Alcaligenes eutrophus H16.




  
PubMed:
        1378052


MedLine:
        




    
Source:
J Bacteriol. 1992 Jul;174(13):4391-400.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1378052









           
Homologs By Global Alignment 

           

       



     
Gene ID:434
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
726
                           PA4147_tr
                           translation of PA4147
                           625
                           99.68
                           99.68
                           0
                           3214.0
     

     
     
32590
                           gb|AAG07534.1|AE004831_6
                           (AE004831) transcriptional regulator AcoR [Pseudomonas aeruginosa PAO1]
                           625
                           99.68
                           99.68
                           0
                           3214.0
     

     
     
32591
                           emb|CAA72019.1|
                           (Y11136) acoR [Pseudomonas putida]
                           625
                           73.76
                           81.12
                           8
                           2305.0
     

     
     
32592
                           gb|AAN66184.1|AE016246_1
                           (AE016776) acetoin catabolism regulatory protein [Pseudomonas putida KT2440]
                           626
                           73.48
                           81.46
                           10
                           2305.0
     

     
     
727
                           PA4021_tr
                           translation of PA4021
                           658
                           46.20
                           58.51
                           48
                           1254.0
     

     






      

          
GC ORFID: 43436How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4147Glimmer Score: 

          
Start: 4067460Stop: 4065583

          
Length: 1878


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4147 translation of PA4147Homolog Bit Score 1242.0

          
Other Homologs:  t_PA4021 (ORF: BLASTX 474.0), t_PA2665 (ORF: BLASTX 235.0), t_PA1663 (ORF: BLASTX 234.0), t_PA1945 (ORF: BLASTX 227.0), t_PA4726 (ORF: BLASTX 221.0), psyr_15may02_Scaffold15_revised_gene2516 (ORF: BLASTX 218.0), psyr_15may02_Scaffold8_revised_gene7916 (ORF: BLASTX 216.0), psyr_15may02_Scaffold2_revised_gene4053 (ORF: BLASTX 215.0), t_PA2449 (ORF: BLASTX 213.0), t_PA2005 (ORF: BLASTX 211.0), t_PA1196 (ORF: BLASTX 202.0), t_PA5166 (ORF: BLASTX 193.0), t_PA1335 (ORF: BLASTX 193.0), psyr_15may02_Scaffold5_revised_gene6135 (ORF: BLASTX 187.0), t_PA5511 (ORF: BLASTX 186.0), psyr_15may02_Scaffold3_revised_gene4481 (ORF: BLASTX 177.0), t_PA4147 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCTTTCCGCACACTCGAAGGACCATGTGGACTGTGTCAGCCGTGTCCTGAGGAACGCCGCGCGCCTGCCG 
CAGGCGCCGGTGCCGACGCCGATCCTCGACTCCTGGCGCCGCTCCATGGAGCAGCACCGTCTCGACCCGGGG 
TCCCTGCAAGGGCCGCGGATTCTCTCGCAGACCCTCCTCGACGAATGTCGCGAGCGTTCCGAACTGTTCCTG 
CGCATCGCCAGCGAAGAGGTGGCGCGACTGCATGGACGGGTGCGCGATGCCGACTACTGCGTACTGCTCACC 
GATGCCCAGGGGCGGACCATCGACTACCGTGTCGAGTCGACCATCCGCAACGACTGCCGCAAGGCCGGGCTG 
TACCTCGGCACCTGCTGGTCGGAAGGCGAGGAGGGCACCTGCGGGGTGGCCGCCGTACTCACCGACAGGACT 
GCGGTGACGGTGCACAAGCGCGATCACTTCCGTGCCGCCTTCATCGACCTGACCTGTTCCGCGGCGCCGGTC 
TTCGATCCGCAGGGGGAGTTGCTCGGTGTGCTCGACGTTTCCGCCGTGCAATCGCCGGACGACCGCCGTAGC 
CAGCATCTGATCCGGCAGATGGTGGCGCAGAGCGCGCGGGAGATCGAGAACGCCTTCTTCATGCACAGCGCC 
CAGGGCCATTGGGTGCTGCGCGCGCACCGTGCGCCGGGCTACGTCGACAGCCAGCCGGACTACCTGTTCGCC 
TGGGACGCCGATGGGCGCCTGCTGGCGTTGAACCCGGCGGCGCGGCACTACCTGCGGCAGCGCCTGGGGCAG 
GTGCCGAGCCACATCGGCGAGGTGTTCGACCTCGACGCCCTGCACGCCGCCGCTGACCAGGCGCCGTGCCTG 
CTGCGCGGCCAGGCCGGCGAGCTGCACGTGCGTCTCAGCGCGCCGCGGGCCAAGGCGCGGCGGCTGGAGCGC 
GAGGTGCCCGACGACGCTGCGCTCGACCCGCGCATCGCCGAAAGCCTGCGCCTGGCGGTGCGGGTCAAGGAC 
CGCAACCTGCCGGTGCTGATCCAGGGCGAGACCGGCGCCGGCAAGGAGGTTTTCGCCCGCCAGTTGCACCAG 
GCCAGCGCCCGTCGCGACAAACCCTTCGTCGCGCTCAACTGCGCGGCGATTCCCGAAAGCCTGATCGAGAGC 
GAGCTGTTCGGCTACGTTGGCGGAGCCTTTACCGGCGCGGCGGCGAAAGGGATGCGCGGCCTGCTGCAGCAG 
GCCGATGGCGGTACCCTGTTCCTCGACGAGATCGGTGACATGCCGCTGGGCCTGCAGACCCGCCTGCTGCGC 
GTGCTGGCCGAGGGCGAGGTGGCGCCGCTGGGGGCTGCGCGCCGGCAGGCTGTGGATATCCAGGTGATCTGC 
GCCACCCACCGCGACCTGGCGGCGCTGGTGGCGGCCGGCGGCTTCCGCGAGGATCTCTACTTCCGCCTCGGC 
GGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGAC 
GAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGcAGCCcctgaATgcctgctCGGCTATCGC 
tggccGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGcggcggcGCCACCCTG 
CaACTCGCCGACCTTCCGGCAGAGCTGcgTGGCGaAGGCCGGACACCGGCCAGTGCGTGCGAGAGCGGCGGC 
GGTCCGGAGCGCGACGCCCTGCTCGACGCCCTCGTGCGGCATCGCTGGAAACCCATGGCCGCGGCGCGGGAA 
CTGGGTATCTCGCGAGCGACCCTGTATCGGCGGGTGCGCCGGCATGGCATCCGCATGCCGGGCCAGGCGGAC 
GACTGA