ViewGene
Locus Name:
GeneID:
Location:

GeneID:433
TrivialName: PA14_02250
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_02250
PAO1 Ortholog Locus:PA0178
Sequence Length:1920
Protein Length:639
Start: 202926
Stop: 201007
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03568.1|AE004455_9 (AE004455) probable two-component sensor [Pseudomonas aeruginosa PAO1]
Identities = 636/639 (99%), Positives = 637/639 (99%)
Sequence:
ATGCCCCAGGATATGAGCCAGTTTCTCCAGGTGTTCTTCGAGGAAACCGAGGAACACCTGGCCACCCTCGAA
CTCCTCCTGATCGGTCTCGACCTGGACCGGCCGGACAGCGAGACGCTGCACGGCATCTTCCGCGCCGCGCAT
TCGATCAAGGGTTCCAGCGGAATGTTCGGTTTCGACGACATCACCGCCGTCACCCACGAACTGGAGACCCTG
CTCGACCGCATCCGCTGCGGCCAGATGCACCTGCGGCCGGACATGATCAGCTCCTTCCTCGAAGCCCGCGAC
GTGCTGCAGCGCCTGCTCGACGCCCATCGCAGCGGCCGTCCCGACCCCGGGGTGCCGTTGCTGGAGACGGTC
GAGCGGCTGCGCGGCTGGCTGCGTGCGCCGGAGCAGGAAGCGGCGGAGGAAGGCTTCGGCCTGTTCGACGAC
GCGCCCGCCAGGGAAACCGCGGACGACGATGCCTTCGGCTTCTTCGACGAAGGCCCGGGCGCTCCGGAACGA
GCGGCCGCGCCGGAAGCGTTCGGGCTCTTCGACGAGGCGCCCGGCGCACCGGCCGCGAGCGAAGCGTTCGGC
CTCTTCGATGACGCGCCGGGCTCCCCGCCCACGGAGGAACGCGCCTTCGGCCTGTTCGACGGCGCCCCCGGC
AGCCCCGCCGCGCCCAGCGCGCCCGCGCAAGCCGTCGCCGCGCCGGCCAGGGGCGCAGTCGCGCCGGTGCGT
GGCGACGGCGAGTCCGGCTCGATCCGGGTCAGCGTGGAAAAGATCGACAGCCTGATCAACCTGGTCGGCGAA
CTGGTGATCACCCAGGCCATGCTCGGCCAGCTCGGCGAGCAGCTCGACCCGAGCCGCCACGAGCGCCTGCAG
CACGCCCTGGCCCAGCTCGAACACAACACCCGCGACCTGCAGGAATCGGTGATGTCGATCCGCATGCTGCCG
ATCAACTTCATCTTCAGCCGCTTCCCCAGGCTGGTGCGCGACACCGCCACGCGCCTGGGCAAGCAGGTCGAG
CTGCACCTGCACGGCGAGCACACCGAACTGGACAAGAGCGTGATCGAGAAACTCAGCGATCCGCTCACCCAC
ATCGTCCGCAACAGCATCGACCACGGCATCGAAACGCCCGCCGAACGCCTCGCCGCCGGCAAGCCGGCGAGC
GGCACGGTGAAGCTCGCCGCCAGCCACCAGGGCGGCAGCGTGGTGGTGGAGGTTTCCGACGACGGTCGCGGC
CTGTCGCGTCCGCGCATCCTCGCCAAGGCCCGCGAGCGCAACCTGCCGGTGCATGACGGGATGAGCGACGCC
GAGGTCTGGCAACTGGTGTTCATGCCCGGCTTCTCCACCGCCGAGACGGTCACCGAGCTGTCCGGACGCGGC
GTCGGCATGGACGTGGTGAAGCGCAACATCGGTGCCATGGGCGGGCGCATCGACATCGACTCGGCGCCCGGC
ATGGGCACTCGCATCGGCATCCGCCTGCCGCTGACCCTGGCGATCCTCGACGGCCTGATCGTCGCCGTCGAA
GCGGTCAACTACGTGATTCCGCTGACCTACATCGTCGAGTCGCTGCAAGCGCGCAGCGACGACGTGCGCGGC
CTCGGCGGCGAAGACAACGCGATGATCCGCGTACGCGGCGAGTACCTGCCGCTGTTCAGCCTGCACGAGCTG
TTGCGCATCGGCGGCGAAGCGCCGGCGCCGGAGCAGGGCATCGTGGTGATCCTCGAGTCCGAGGGGCGCAGC
TTCGCCCTGCAGGTGGACGAACTGGTCGGCCAGCAGCAGGTGGTGATCAAGAGCCTGGAACAGAATTTCCGC
CGCGTGGAGGGTATCGCCGGCGCCACCATCATGGGCGACGGCAGCGTCGCCCTGATTCTCGACGTCGATGCG
CTGCCACGCCTGGCGGCGCGGGAGGACACCGCCGATGAACGGCACTGA
Translation:
MPQDMSQFLQVFFEETEEHLATLELLLIGLDLDRPDSETLHGIFRAAHSIKGSSGMFGFDDITAVTHELETL
LDRIRCGQMHLRPDMISSFLEARDVLQRLLDAHRSGRPDPGVPLLETVERLRGWLRAPEQEAAEEGFGLFDD
APARETADDDAFGFFDEGPGAPERAAAPEAFGLFDEAPGAPAASEAFGLFDDAPGSPPTEERAFGLFDGAPG
SPAAPSAPAQAVAAPARGAVAPVRGDGESGSIRVSVEKIDSLINLVGELVITQAMLGQLGEQLDPSRHERLQ
HALAQLEHNTRDLQESVMSIRMLPINFIFSRFPRLVRDTATRLGKQVELHLHGEHTELDKSVIEKLSDPLTH
IVRNSIDHGIETPAERLAAGKPASGTVKLAASHQGGSVVVEVSDDGRGLSRPRILAKARERNLPVHDGMSDA
EVWQLVFMPGFSTAETVTELSGRGVGMDVVKRNIGAMGGRIDIDSAPGMGTRIGIRLPLTLAILDGLIVAVE
AVNYVIPLTYIVESLQARSDDVRGLGGEDNAMIRVRGEYLPLFSLHELLRIGGEAPAPEQGIVVILESEGRS
FALQVDELVGQQQVVIKSLEQNFRRVEGIAGATIMGDGSVALILDVDALPRLAAREDTADERH*
AnnotationID:442GeneID:433
AnnotatorUID: diggins
Modification Date/Time: 2005-05-08 15:03:44
Gene Name:cheA
Confidence Code:2
GeneProduct:putative two-component sensor
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(28) Two-component regulatory systems
Alternate Gene Product Name:putative chemotaxis protein CheA
Functional Category Confidence Code:5
COGs:cd00731
Secondary Functional Category(ies):signal transduction mechanism
EC Number:2.7.3.-
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAE36774.1| (BX640427) chemotaxis protein CheA [Bordetella parapertussis]
          Length = 685

 Score =  560 bits (1442), Expect = e-158
 Identities = 330/679 (48%), Positives = 420/679 (61%), Gaps = 68/679 (10%)

>gb|AAL20837.1| (AE008785) sensory histitine protein kinase [Salmonella typhimurium
           LT2]
          Length = 671

 Score =  531 bits (1367), Expect = e-150
 Identities = 319/668 (47%), Positives = 410/668 (61%), Gaps = 48/668 (7%)








 
Structural Features:
cd00731, CheA_reg, CheA regulatory domain
CD-Length = 132 residues, 100.0% aligned
Score =  142 bits (361), Expect = 1e-34
Identities = 63/133 (47%), Positives = 89/133 (66%), Gaps = 1/133 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:869




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18011




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33548




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:433_cdd.html


    		




 
URL:

















    ReferenceID:35488




  
Author/Investigator(s):
        Kato J, Nakamura T, Kuroda A, Ohtake H.





  
Title:
        Cloning and characterization of chemotaxis genes in Pseudomonas aeruginosa.




  
PubMed:
        10052136


MedLine:
        




    
Source:
Biosci Biotechnol Biochem. 1999 Jan;63(1):155-61.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10052136









           
Homologs By Global Alignment 

           

       



     
Gene ID:433
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
725
                           PA0178_tr
                           translation of PA0178
                           639
                           99.53
                           99.68
                           0
                           3220.0
     

     
     
32526
                           gb|AAG03568.1|AE004455_9
                           (AE004455) probable two-component sensor [Pseudomonas aeruginosa PAO1]
                           639
                           99.53
                           99.68
                           0
                           3220.0
     

     
     
32530
                           emb|CAE41328.1|
                           (BX640414) chemotaxis protein CheA [Bordetella pertussis] [Bordetella pertussis Tohama I]
                           700
                           48.28
                           60.85
                           76
                           1471.0
     

     
     
32528
                           emb|CAE36774.1|
                           (BX640427) chemotaxis protein CheA [Bordetella parapertussis]
                           704
                           48.15
                           60.79
                           84
                           1487.0
     

     
     
32529
                           emb|CAE33040.1|
                           (BX640444) chemotaxis protein CheA [Bordetella bronchiseptica] [Bordetella bronchiseptica RB50]
                           706
                           48.01
                           60.62
                           86
                           1482.0
     

     






      

          
GC ORFID: 43435How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0178Glimmer Score: 

          
Start: 6004265Stop: 6002346

          
Length: 1920


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0178 translation of PA0178Homolog Bit Score 1244.0

          
Other Homologs:  t_PA0178 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCCCAGGATATGAGCCAGTTTCTCCAGGTGTTCTTCGAGGAAACCGAGGAACACCTGGCCACCCTCGAA 
CTCCTCCTGATCGGTCTCGACCTGGACCGGCCGGACAGCGAGACGCTGCACGGCATCTTCCGCGCCGCGCAT 
TCGATCAAGGGTTCCAGCGGAATGTTCGGTTTCGACGACATCACCGCCGTCACCCACGAACTGGAGACCCTG 
CTCGACCGCATCCGCTGCGGCCAGATGCACCTGCGGCCGGACATGATCAGCTCCTTCCTCGAAGCCCGCGAC 
GTGCTGCAGCGCCTGCTCGACGCCCATCGCAGCGGCCGTCCCGACCCCGGGGTGCCGTTGCTGGAGACGGTC 
GAGCGGCTGCGCGGCTGGCTGCGTGCGCCGGAGCAGGAAGCGGCGGAGGAAGGCTTCGGCCTGTTCGACGAC 
GCGCCCGCCAGGGAAACCGCGGACGACGATGCCTTCGGCTTCTTCGACGAAGGCCCGGGCGCTCCGGAACGA 
GCGGCCGCGCCGGAAGCGTTCGGGCTCTTCGACGAGGCGCCCGGCGCACCGGCCGCGAGCGAAGCGTTCGGC 
CTCTTCGATGACGCGCCGGGCTCCCCGCCCACGGAGGAACGCGCCTTCGGCCTGTTCGACGGCGCCCCCGGC 
AGCCCCGCCGCGCCCAGCGCGCCCGCGCAAGCCGTCGCCGCGCCGGCCAGGGGCGCAGTCGCGCCGGTGCGT 
GGCGACGGCGAGTCCGGCTCGATCCGGGTCAGCGTGGAAAAGATCGACAGCCTGATCAACCTGGTCGGCGAA 
CTGGTGATCACCCAGGCCATGCTCGGCCAGCTCGGCGAGCAGCTCGACCCGAGCCGCCACGAGCGCCTGCAG 
CACGCCCTGGCCCAGCTCGAACACAACACCCGCGACCTGCAGGAATCGGTGATGTCGATCCGCATGCTGCCG 
ATCAACTTCATCTTCAGCCGCTTCCCCAGGCTGGTGCGCGACACCGCCACGCGCCTGGGCAAGCAGGTCGAG 
CTGCACCTGCACGGCGAGCACACCGAACTGGACAAGAGCGTGATCGAGAAACTCAGCGATCCGCTCACCCAC 
ATCGTCCGCAACAGCATCGACCACGGCATCGAAACGCCCGCCGAACGCCTCGCCGCCGGCAAGCCGGCGAGC 
GGCACGGTGAAGCTCGCCGCCAGCCACCAGGGCGGCAGCGTGGTGGTGGAGGTTTCCGACGACGGTCGCGGC 
CTGTCGCGTCCGCGCATCCTCGCCAAGGCCCGCGAGCGCAACCTGCCGGTGCATGACGGGATGAGCGACGCC 
GAGGTCTGGCAACTGGTGTTCATGCCCGGCTTCTCCACCGCCGAGACGGTCACCGAGCTGTCCGGACGCGGC 
GTCGGCATGGACGTGGTGAAGCGCAACATCGGTGCCATGGGCGGGCGCATCGACATCGACTCGGCGCCCGGC 
ATGGGCACTCGCATCGGCATCCGCCTGCCGCTGACCCTGGCGATCCTCGACGGCCTGATCGTCGCCGTCGAA 
GCGGTCAACTACGTGATTCCGCTGACCTACATCGTCGAGTCGCTGCAAGCGCGCAGCGACGACGTGCGCGGC 
CTCGGCGGCGAAGACAACGCGATGATCCGCGTACGCGGCGAGTACCTGCCGCTGTTCAGCCTGCACGAGCTG 
TTGCGCATCGGCGGCGAAGCGCCGGCGCCGGAGCAGGGCATCGTGGTGATCCTCGAGTCCGAGGGGCGCAGC 
TTCGCCCTGCAGGTGGACGAACTGGTCGGCCAGCAGCAGGTGGTGATCAAGAGCCTGGAACAGAATTTCCGC 
CGCGTGGAGGGTATCGCCGGCGCCACCATCATGGGCGACGGCAGCGTCGCCCTGATTCTCGACGTCGATGCG 
CTGCCACGCCTGGCGGCGCGGGAGGACACCGCCGATGAACGGCACTGA