ViewGene
Locus Name:
GeneID:
Location:

GeneID:4315
TrivialName: PA14_42720
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_42720
PAO1 Ortholog Locus:
Sequence Length:678
Protein Length:225
Start: 3800420
Stop: 3799743
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05074.1|AE004595_13 (AE004595) enolase-phosphatase E-1 [Pseudomonas aeruginosa PAO1]
Identities = 215/218 (98%), Positives = 215/218 (98%)
Sequence:
ATGACCATCAAAGCCATCCTCACCGACATCGAAGGCACCACCAGCGCGGTCAGCTTCGTTTTCGACGTGCTG
TTTCCCTATGCCGCCCGGCACCTGCCGGACTTCGTCCGCGAACACGCCGGGGAGCCCGAGGTAGCCGCGCAA
CTGGCGGCGGTGCGTGCCGAGAGCGGTGAGGCGGACGCCGATGTCGAGCGGGCGATCGCGATCCTCCTGCAA
TGGATCGCCGAGGACCGCAAGGCGACGCCGCTGAAGGCCCTGCAAGGCATGGTCTGGGCCCAGGGCTACCGC
GACGGTCAATTGAAGGGCCACGTCTACCCGGACGCGGTGCAGGCGCTGCGCGAGTGGAAGGCCCGTGGGCTG
GACCTGTACGTCTATTCCTCCGGCTCGATCCAGGCGCAGAAGCTGATCTTCGGCTGTTCCGAGGCCGGCGAC
CTGGGCCCGCTGTTTTCCGGCTACTTCGACACTACCAGCGGGCCGAAGCGGGAAAGCGCCTCCTATGCGCGG
ATCGCCGGGGCCATCGGCCTGCCGGCCGCGGAAATCCTTTTCCTTTCCGACGTGGTGCAGGAGCTGGATGCC
GCGCGCGACGCGGGCATGCGCACGCTCGGCCTGGCCCGCGAAGGCGGCAGCCTGGACGGCCATCCGACGGTG
GCCAGCTTCGCCGACATCGTCGTGGAGTGA
Translation:
MTIKAILTDIEGTTSAVSFVFDVLFPYAARHLPDFVREHAGEPEVAAQLAAVRAESGEADADVERAIAILLQ
WIAEDRKATPLKALQGMVWAQGYRDGQLKGHVYPDAVQALREWKARGLDLYVYSSGSIQAQKLIFGCSEAGD
LGPLFSGYFDTTSGPKRESASYARIAGAIGLPAAEILFLSDVVQELDAARDAGMRTLGLAREGGSLDGHPTV
ASFADIVVE*
AnnotationID:4321GeneID:4315
AnnotatorUID: danlee
Modification Date/Time: 2006-02-06 17:05:04
Gene Name:masA
Confidence Code:2
GeneProduct:enolase-phosphatase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG4229
Secondary Functional Category(ies):energy production and conversion
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO55565.1| (AE016863) HAD-superfamily hydrolase [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 227

 Score =  330 bits (847), Expect = 3e-90
 Identities = 168/219 (76%), Positives = 183/219 (83%)

>gb|AAM41107.1| (AE012284) enolase-phosphatase [Xanthomonas campestris pv.
           campestris str. ATCC 33913]
          Length = 232

 Score =  234 bits (597), Expect = 3e-61
 Identities = 128/219 (58%), Positives = 149/219 (68%), Gaps = 5/219 (2%)

>gb|AAM36700.1| (AE011817) enolase-phosphatase [Xanthomonas axonopodis pv. citri
           str. 306]
          Length = 232

 Score =  227 bits (579), Expect = 4e-59
 Identities = 123/219 (56%), Positives = 147/219 (67%), Gaps = 5/219 (2%)








 
Structural Features:
COG4229, COG4229, Predicted enolase-phosphatase 
[Energy production and conversion]
CD-Length = 229 residues, 100.0% aligned
Score =  331 bits (851), Expect = 3e-92
Identities = 135/230 (58%), Positives = 162/230 (70%), Gaps = 6/230 (2%)






   
Genomic Context:






   
Comment:
215/218 (98%) identical to PAO1 masA (PA1685) in first 218 aa of each protein.
Polymorphism causing frame-shift in PA14 confirmed.


















    ReferenceID:8631




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:25774




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37218




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4315_cdd.html


    		




 
URL:

















    ReferenceID:37873




  
Author/Investigator(s):
        Balakrishnan R, Frohlich M, Rahaim PT, Backman K, Yocum RR.





  
Title:
        Appendix. Cloning and sequence of the gene encoding enzyme E-1 from the methionine salvage pathway of Klebsiella oxytoca.




  
PubMed:
        8227040


MedLine:
        




    
Source:
J Biol Chem. 1993 Nov 25;268(33):24792-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=94043338&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:4315
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6635
                           PA1685_tr
                           translation of PA1685
                           249
                           86.34
                           86.34
                           24
                           1074.0
     

     
     
178942
                           gb|AAG05074.1|AE004595_13
                           (AE004595) enolase-phosphatase E-1 [Pseudomonas aeruginosa PAO1]
                           249
                           86.34
                           86.34
                           24
                           1074.0
     

     
     
178943
                           gb|AAO55565.1|
                           (AE016863) HAD-superfamily hydrolase [Pseudomonas syringae pv. tomato str. DC3000]
                           229
                           73.36
                           81.22
                           6
                           842.0
     

     
     
178944
                           gb|AAM41107.1|
                           (AE012284) enolase-phosphatase [Xanthomonas campestris pv. campestris str. ATCC 33913]
                           234
                           55.55
                           64.52
                           11
                           598.0
     

     
     
178945
                           gb|AAM36700.1|
                           (AE011817) enolase-phosphatase [Xanthomonas axonopodis pv. citri str. 306]
                           238
                           53.78
                           64.28
                           19
                           585.0
     

     






      

          
GC ORFID: 47317How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: psyr_15may02_Scaffold1_revised_gene859Glimmer Score: 

          
Start: 1153489Stop: 1154166

          
Length: 678


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: psyr_15may02_Scaffold1_revised_gene859 related to: gi|15596882|ref|NP_250376.1| (NC_002516) enolase-phosphatase E-1 [Pseudomonas aeruginosa] pir||F83436 enolase-phosphatase E-1 PA1685 [imported] - Pseudomonas aeruginosa (strain PAO1) gb|AAG05074.1|AE00459Homolog Bit Score 329.0

          
Other Homologs:  t_PA1685 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCATCAAAGCCATCCTCACCGACATCGAAGGCACCACCAGCGCGGTCAGCTTCGTTTTCGACGTGCTG 
TTTCCCTATGCCGCCCGGCACCTGCCGGACTTCGTCCGCGAACACGCCGGGGAGCCCGAGGTAGCCGCGCAA 
CTGGCGGCGGTGCGTGCCGAGAGCGGTGAGGCGGACGCCGATGTCGAGCGGGCGATCGCGATCCTCCTGCAA 
TGGATCGCCGAGGACCGCAAGGCGACGCCGCTGAAGGCCCTGCAAGGCATGGTCTGGGCCCAGGGCTACCGC 
GACGGTCAATTGAAGGGCCACGTCTACCCGGACGCGGTGCAGGCGCTGCGCGAGTGGAAGGCCCGTGGGCTG 
GACCTGTACGTCTATTCCTCCGGCTCGATCCAGGCGCAGAAGCTGATCTTCGGCTGTTCCGAGGCCGGCGAC 
CTGGGCCCGCTGTTTTCCGGCTACTTCGACACTACCAGCGGGCCGAAGCGGGAAAGCGCCTCCTATGCGCGG 
ATCGCCGGGGCCATCGGCCTGCCGGCCGCGGAAATCCTTTTCCTTTCCGACGTGGTGCAGGAGCTGGATGCC 
GCGCGCGACGCGGGCATGCGCACGCTCGGCCTGGCCCGCGAAGGCGGCAGCCTGGACGGCCATCCGACGGTG 
GCCAGCTTCGCCGACATCGTCGTGGAGTGA