ViewGene
Locus Name:
GeneID:
Location:

GeneID:430
TrivialName: PA14_57710
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_57710
PAO1 Ortholog Locus:PA4442
Sequence Length:1902
Protein Length:633
Start: 5140186
Stop: 5138285
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07830.1|AE004858_8 (AE004858) ATP sulfurylase GTP-binding subunit/APS kinase [Pseudomonas aeruginosa PAO1]
Identities = 633/633 (100%), Positives = 633/633 (100%)
Sequence:
ATGTCTCATCAATCCGATCTGATCAGCGAGGACATCCTCGCCTACCTGGGGCAGCACGAACGCAAGGAACTG
CTGCGCTTCCTTACCTGCGGCAACGTCGATGACGGCAAGAGCACCCTGATCGGGCGCCTGCTGCATGACTCC
AAGATGATCTACGAGGATCACCTCGAGGCCATCACCCGCGACTCGAAGAAAGTCGGCACCACGGGCGACGAC
GTCGACCTGGCGCTGCTGGTCGACGGCCTGCAGGCCGAGCGCGAGCAGGGCATCACCATCGACGTGGCGTAC
CGCTACTTCAGCACCGCCAAGCGCAAGTTCATCATCGCCGACACGCCCGGCCATGAGCAGTACACCCGCAAC
ATGGCCACCGGTGCCTCGACCTGCGACCTGGCGATCATCCTGATCGATGCCCGCTACGGCGTGCAGACCCAG
ACCCGCCGGCACAGTTTCATCGCGTCCCTGCTGGGCATCCGGCATATCGTGGTCGCCATCAACAAGATGGAC
CTGAAGGACTTCGACCAGGGTGTCTTCGAACAGATCAAGGCCGACTACCTGGCCTTTGCCGAGAAGATCGGT
CTGAAGACCAGTTCGCTGCACTTCGTGCCGATGTCGGCGCTCAAGGGCGACAACGTGGTCAACAAGTCCGAG
CGTTCGCCCTGGTACGCCGGCCAGTCGCTGATGGAAATCCTCGAAACCGTCGAGATCGCCGCCGACCGCAAC
CTCGATGACATGCGCTTCCCGGTGCAGTACGTGAACCGGCCGAACCTGAACTTCCGCGGTTTCGCCGGCACC
CTGGCCAGCGGCGTGGTGCGCAAGGGCGACGAGGTGGTGGCCTTGCCGTCGGGCAAGGGCAGCAAGGTGAAA
TCCATCGTCACCTTCGAGGGCGAACTGGAGCAGGCCGGTCCCGGCCAGGCCGTCACCCTGACCCTGGAAGAC
GAGATCGACGTTTCCCGCGGCGACATGCTGGTGCATGCCGACAATCGCCCGCTGGTCACCGACGGCTTCGAT
GCCATGCTGGTGTGGATGGCCGAGGAGCCGATGCTTCCGGGCAAGAAGTACGATATCAAGCGCGCTACCAGC
TATGTGCCGGGTTCGATTCCGAGCATCGTCCACAAGGTGGACGTGAATACCCTGGAGCGGACCCCGGGCAGC
GAGCTGAAGCTGAACGAGATCGCCAGGGTCAAGGTCAGCCTGGACGCGCCCATCGCGCTCGATGGCTATGAG
CAGAACCGCACCACCGGCGCCTTCATCGTCATCGATCGCCTGACCAACGGCACCGTTGGCGCCGGCATGATC
GTCTCCGCTCCGCCGGCGGCCCATGGCAGCAGCGCGCATCACGGTTCGAATGCCCATGTGACGCGCGAGGAG
CGTGCCGGCCGCTTCGGCCAGCAGCCGGCGACCGTACTGTTCAGCGGCCTCTCCGGCGCCGGCAAGAGCACC
CTGGCCTACGCCGTGGAGCGCAAGCTGTTCGATATGGGCCGCGCGGTCTACGTGCTGGACGGGCAGAACCTG
CGCCACGACCTGAACAAGGGGTTGCCGCAGGACCGCGCCGGGCGCACCGAGAACTGGCTGCGTACCGCCCAT
GTCGCCAAGCAGTTCAACGAGGCCGGGTTGATCAGCCTCTGCGCCTTCGTCGCGCCGAGCGCGGAAGGGCGC
GAGCAGGCCAGGGCGCTGATCGGCGCCGAGCGCCTGATCACCGTCTATGTGCAGGCGTCGCCGCAGGTCTGC
CGCGAACGCGATCCCCAGGGGCTGTATGCGGCGGGCGAGGACAATATCCCCGGCGAGTCCTTCCCCTACGAC
GTGCCGCTGGATGCCGACCTGGTGATCGATACCCAGGCGCTGAGCGTCGAGGACGGCGTGAAGCAGGTGCTC
GACCTGTTGCGCGAACGCCAGGCGATCTGA
Translation:
MSHQSDLISEDILAYLGQHERKELLRFLTCGNVDDGKSTLIGRLLHDSKMIYEDHLEAITRDSKKVGTTGDD
VDLALLVDGLQAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRNMATGASTCDLAIILIDARYGVQTQ
TRRHSFIASLLGIRHIVVAINKMDLKDFDQGVFEQIKADYLAFAEKIGLKTSSLHFVPMSALKGDNVVNKSE
RSPWYAGQSLMEILETVEIAADRNLDDMRFPVQYVNRPNLNFRGFAGTLASGVVRKGDEVVALPSGKGSKVK
SIVTFEGELEQAGPGQAVTLTLEDEIDVSRGDMLVHADNRPLVTDGFDAMLVWMAEEPMLPGKKYDIKRATS
YVPGSIPSIVHKVDVNTLERTPGSELKLNEIARVKVSLDAPIALDGYEQNRTTGAFIVIDRLTNGTVGAGMI
VSAPPAAHGSSAHHGSNAHVTREERAGRFGQQPATVLFSGLSGAGKSTLAYAVERKLFDMGRAVYVLDGQNL
RHDLNKGLPQDRAGRTENWLRTAHVAKQFNEAGLISLCAFVAPSAEGREQARALIGAERLITVYVQASPQVC
RERDPQGLYAAGEDNIPGESFPYDVPLDADLVIDTQALSVEDGVKQVLDLLRERQAI*
AnnotationID:439GeneID:430
AnnotatorUID: diggins
Modification Date/Time: 2005-05-02 21:09:33
Gene Name:cysN
Confidence Code:1
GeneProduct:ATP sulfurylase GTP-binding subunit/APS kinase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG2895,COG5256,COG0529
Secondary Functional Category(ies):
EC Number:2.7.7.4
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66928.1|AE016321_4 (AE016778) sulfate adenylyltransferase, subunit 1/adenylylsulfate
           kinase [Pseudomonas putida KT2440]
          Length = 633

 Score = 1097 bits (2836), Expect = 0.0
 Identities = 548/633 (86%), Positives = 586/633 (92%)

>gb|AAO57881.1| (AE016872) sulfate adenylate transferase, subunit 1/adenylylsulfate
           kinase [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 632

 Score = 1091 bits (2822), Expect = 0.0
 Identities = 552/633 (87%), Positives = 582/633 (91%), Gaps = 1/633 (0%)






 
Structural Features:
COG2895, CysN, GTPases - Sulfate adenylate transferase subunit 1 
[Inorganic ion transport and metabolism]
CD-Length = 431 residues, 100.0% aligned
Score =  697 bits (1800), Expect = 0.0
Identities = 291/433 (67%), Positives = 342/433 (78%), Gaps = 2/433 (0%)

COG5256, TEF1, Translation elongation factor EF-1alpha (GTPase) 
[Translation, ribosomal structure and biogenesis]
CD-Length = 428 residues,  98.6% aligned
Score =  297 bits (762), Expect = 5e-81
Identities = 146/427 (34%), Positives = 231/427 (54%), Gaps = 18/427 (4%)

COG0529, CysC, Adenylylsulfate kinase and related kinases 
[Inorganic ion transport and metabolism]
CD-Length = 197 residues,  99.0% aligned
Score =  255 bits (653), Expect = 2e-68
Identities = 91/195 (46%), Positives = 119/195 (61%), Gaps = 4/195 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:863




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18005




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31481




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:430_cdd.html


    		




 
URL:

















    ReferenceID:32827




  
Author/Investigator(s):
        Kertesz MA, Schmidt-Larbig K, Wuest T.





  
Title:
        A novel reduced flavin mononucleotide-dependent methanesulfonate sulfonatase encoded by the sulfur-regulated msu operon of Pseudomonas aeruginosa.




  
PubMed:
        10049377


MedLine:
        




    
Source:
J Bacteriol. 1999 Mar;181(5):1464-73.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10049377

















    ReferenceID:32828




  
Author/Investigator(s):
        Hummerjohann J, Kuttel E, Quadroni M, Ragaller J, Leisinger T, Kertesz MA.





  
Title:
        Regulation of the sulfate starvation response in Pseudomonas aeruginosa: role of cysteine biosynthetic intermediates.




  
PubMed:
        9611812


MedLine:
        




    
Source:
Microbiology. 1998 May;144 ( Pt 5):1375-86.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9611812









           
Homologs By Global Alignment 

           

       



     
Gene ID:430
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
722
                           PA4442_tr
                           translation of PA4442
                           633
                           99.99
                           99.99
                           0
                           3222.0
     

     
     
32317
                           gb|AAG07830.1|AE004858_8
                           (AE004858) ATP sulfurylase GTP-binding subunit/APS kinase [Pseudomonas aeruginosa PAO1]
                           633
                           99.99
                           99.99
                           0
                           3222.0
     

     
     
32318
                           gb|AAC46387.1|
                           (AF035608) ATP sulfurylase GTP-binding subunit/APS kinase [Pseudomonas aeruginosa]
                           633
                           99.99
                           99.99
                           0
                           3222.0
     

     
     
32320
                           gb|AAO57881.1|
                           (AE016872) sulfate adenylate transferase, subunit 1/adenylylsulfate kinase [Pseudomonas syringae pv. tomato str. DC3000]
                           633
                           87.20
                           91.94
                           1
                           2824.0
     

     
     
32319
                           gb|AAN66928.1|AE016321_4
                           (AE016778) sulfate adenylyltransferase, subunit 1/adenylylsulfate kinase [Pseudomonas putida KT2440]
                           633
                           86.57
                           92.57
                           0
                           2836.0
     

     






      

          
GC ORFID: 43432How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4442Glimmer Score: 

          
Start: 4403865Stop: 4401964

          
Length: 1902


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4442 translation of PA4442Homolog Bit Score 1245.0

          
Other Homologs:  psyr_15may02_Scaffold12_revised_gene2025 (ORF: BLASTX 1085.0), t_PA4442 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCTCATCAATCCGATCTGATCAGCGAGGACATCCTCGCCTACCTGGGGCAGCACGAACGCAAGGAACTG 
CTGCGCTTCCTTACCTGCGGCAACGTCGATGACGGCAAGAGCACCCTGATCGGGCGCCTGCTGCATGACTCC 
AAGATGATCTACGAGGATCACCTCGAGGCCATCACCCGCGACTCGAAGAAAGTCGGCACCACGGGCGACGAC 
GTCGACCTGGCGCTGCTGGTCGACGGCCTGCAGGCCGAGCGCGAGCAGGGCATCACCATCGACGTGGCGTAC 
CGCTACTTCAGCACCGCCAAGCGCAAGTTCATCATCGCCGACACGCCCGGCCATGAGCAGTACACCCGCAAC 
ATGGCCACCGGTGCCTCGACCTGCGACCTGGCGATCATCCTGATCGATGCCCGCTACGGCGTGCAGACCCAG 
ACCCGCCGGCACAGTTTCATCGCGTCCCTGCTGGGCATCCGGCATATCGTGGTCGCCATCAACAAGATGGAC 
CTGAAGGACTTCGACCAGGGTGTCTTCGAACAGATCAAGGCCGACTACCTGGCCTTTGCCGAGAAGATCGGT 
CTGAAGACCAGTTCGCTGCACTTCGTGCCGATGTCGGCGCTCAAGGGCGACAACGTGGTCAACAAGTCCGAG 
CGTTCGCCCTGGTACGCCGGCCAGTCGCTGATGGAAATCCTCGAAACCGTCGAGATCGCCGCCGACCGCAAC 
CTCGATGACATGCGCTTCCCGGTGCAGTACGTGAACCGGCCGAACCTGAACTTCCGCGGTTTCGCCGGCACC 
CTGGCCAGCGGCGTGGTGCGCAAGGGCGACGAGGTGGTGGCCTTGCCGTCGGGCAAGGGCAGCAAGGTGAAA 
TCCATCGTCACCTTCGAGGGCGAACTGGAGCAGGCCGGTCCCGGCCAGGCCGTCACCCTGACCCTGGAAGAC 
GAGATCGACGTTTCCCGCGGCGACATGCTGGTGCATGCCGACAATCGCCCGCTGGTCACCGACGGCTTCGAT 
GCCATGCTGGTGTGGATGGCCGAGGAGCCGATGCTTCCGGGCAAGAAGTACGATATCAAGCGCGCTACCAGC 
TATGTGCCGGGTTCGATTCCGAGCATCGTCCACAAGGTGGACGTGAATACCCTGGAGCGGACCCCGGGCAGC 
GAGCTGAAGCTGAACGAGATCGCCAGGGTCAAGGTCAGCCTGGACGCGCCCATCGCGCTCGATGGCTATGAG 
CAGAACCGCACCACCGGCGCCTTCATCGTCATCGATCGCCTGACCAACGGCACCGTTGGCGCCGGCATGATC 
GTCTCCGCTCCGCCGGCGGCCCATGGCAGCAGCGCGCATCACGGTTCGAATGCCCATGTGACGCGCGAGGAG 
CGTGCCGGCCGCTTCGGCCAGCAGCCGGCGACCGTACTGTTCAGCGGCCTCTCCGGCGCCGGCAAGAGCACC 
CTGGCCTACGCCGTGGAGCGCAAGCTGTTCGATATGGGCCGCGCGGTCTACGTGCTGGACGGGCAGAACCTG 
CGCCACGACCTGAACAAGGGGTTGCCGCAGGACCGCGCCGGGCGCACCGAGAACTGGCTGCGTACCGCCCAT 
GTCGCCAAGCAGTTCAACGAGGCCGGGTTGATCAGCCTCTGCGCCTTCGTCGCGCCGAGCGCGGAAGGGCGC 
GAGCAGGCCAGGGCGCTGATCGGCGCCGAGCGCCTGATCACCGTCTATGTGCAGGCGTCGCCGCAGGTCTGC 
CGCGAACGCGATCCCCAGGGGCTGTATGCGGCGGGCGAGGACAATATCCCCGGCGAGTCCTTCCCCTACGAC 
GTGCCGCTGGATGCCGACCTGGTGATCGATACCCAGGCGCTGAGCGTCGAGGACGGCGTGAAGCAGGTGCTC 
GACCTGTTGCGCGAACGCCAGGCGATCTGA