ViewGene
Locus Name:
GeneID:
Location:

GeneID:4291
TrivialName: PA14_10990
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_10990
PAO1 Ortholog Locus:PA4092
Sequence Length:513
Protein Length:170
Start: 951014
Stop: 950502
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07479.1|AE004825_8 (AE004825) 4-hydroxyphenylacetate 3-monooxygenase small chain [Pseudomonas aeruginosa PAO1]
Identities = 167/170 (98%), Positives = 167/170 (98%)
Sequence:
ATGTCCCAGCTCGAACCCAGGCAGCAAGCCTTCCGCAACGCCATGGCGCATCTTTCGGCGGCGGTCAACGTG
ATCACCAGCAACGGCCCGGCCGGACGCTGCGGGATCACCGCCACCGCGGTCTGCTCGGTCACCGACAGCCCG
CCGACGCTGATGCTCTGCATCAACCGCAACAGCGAGATGAACACGGTGTTCAAGGCCAACGGCCGGCTCTGC
GTGAACGTCCTCAGCGGCGAACACGAAGAGGTGGCCCGCCACTTCGCCGGCATGACCGAGGTCCCGATGGAA
CGCCGCTTCGCCCTCCACGACTGGCGCGAGGGCCTCGCCGGGTTGCCGGTGCTGCACGGCGCCCTGGCCAAC
CTGCAGGGACGCATCGCCGAGGTCCAGGAGATCGGCACCCATTCGGTGCTGTTGCTGGAGCTGGAGGACATC
CAGGTCCTCGAACAGGGCGACGGCCTGGTCTACTTCAGCCGCAGCTTCCATCGCCTGCAGCGAGCCTGCCGA
GCCGCCTGA
Translation:
MSQLEPRQQAFRNAMAHLSAAVNVITSNGPAGRCGITATAVCSVTDSPPTLMLCINRNSEMNTVFKANGRLC
VNVLSGEHEEVARHFAGMTEVPMERRFALHDWREGLAGLPVLHGALANLQGRIAEVQEIGTHSVLLLELEDI
QVLEQGDGLVYFSRSFHRLQRACRAA*
AnnotationID:4297GeneID:4291
AnnotatorUID: diggins
Modification Date/Time: 2005-03-30 10:10:06
Gene Name:hpaC
Confidence Code:2
GeneProduct:4-hydroxyphenylacetate 3-monooxygenase small chain
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:component C of the 4HPA-hydroxylase
Functional Category Confidence Code:5
COGs:pfam01613,COG1853
Secondary Functional Category(ies):
EC Number:1.6.8.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO17198.1| (AF346500) HpaC [Photorhabdus luminescens]
          Length = 172

 Score =  205 bits (521), Expect = 1e-52
 Identities = 101/170 (59%), Positives = 128/170 (75%)

>emb|CAA82322.2| (Z29081) flavin:NADH oxidoreductase component of the 4-HPA
           3-monooxygenase [Escherichia coli]
          Length = 170

 Score =  199 bits (505), Expect = 8e-51
 Identities = 98/168 (58%), Positives = 128/168 (76%)

>gb|AAP19569.1| (AE016993) component C of the 4HPA-hydroxylase [Shigella flexneri
           2a str. 2457T]
          Length = 170

 Score =  197 bits (500), Expect = 3e-50
 Identities = 97/168 (57%), Positives = 127/168 (75%)






 
Structural Features:
pfam01613, Flavin_Reduct, Flavin reductase like domain.
CD-Length = 149 residues,  98.0% aligned
Score =  153 bits (388), Expect = 1e-38
Identities = 55/147 (37%), Positives = 81/147 (55%), Gaps = 1/147 (0%)

COG1853, Conserved protein/domain typically associated with flavoprotein oxygenases, 
DIM6/NTAB family
CD-Length = 176 residues,  90.3% aligned
Score =  142 bits (360), Expect = 2e-35
Identities = 56/161 (34%), Positives = 89/161 (55%), Gaps = 5/161 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:8583




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:25726




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36840




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4291_cdd.html


    		




 
URL:

















    ReferenceID:36947




  
Author/Investigator(s):
        Prieto MA, Diaz E, Garcia JL.





  
Title:
        Molecular characterization of the 4-hydroxyphenylacetate catabolic pathway of Escherichia coli W: engineering a mobile aromatic degradative cluster.




  
PubMed:
        8550403


MedLine:
        




    
Source:
J Bacteriol. 1996 Jan;178(1):111-20.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8550403

















    ReferenceID:36948




  
Author/Investigator(s):
        Prieto MA, Garcia JL.





  
Title:
        Molecular characterization of 4-hydroxyphenylacetate 3-hydroxylase of Escherichia coli. A two-protein component enzyme.




  
PubMed:
        8077235


MedLine:
        




    
Source:
J Biol Chem. 1994 Sep 9;269(36):22823-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=94357932&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:4291
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6603
                           PA4092_tr
                           translation of PA4092
                           170
                           98.23
                           98.23
                           0
                           850.0
     

     
     
178347
                           gb|AAG07479.1|AE004825_8
                           (AE004825) 4-hydroxyphenylacetate 3-monooxygenase small chain [Pseudomonas aeruginosa PAO1]
                           170
                           98.23
                           98.23
                           0
                           850.0
     

     
     
178348
                           gb|AAO17198.1|
                           (AF346500) HpaC [Photorhabdus luminescens]
                           172
                           58.72
                           74.41
                           2
                           521.0
     

     
     
178350
                           emb|CAA82322.2|
                           (Z29081) flavin:NADH oxidoreductase component of the 4-HPA 3-monooxygenase [Escherichia coli]
                           171
                           57.30
                           74.85
                           2
                           504.0
     

     
     
178351
                           emb|CAA86049.2|
                           (Z37980) component C of the 4HPA-hydroxylase [Escherichia coli]
                           171
                           57.30
                           74.85
                           2
                           504.0
     

     






      

          
GC ORFID: 47293How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4092Glimmer Score: 

          
Start: 4002923Stop: 4003435

          
Length: 513


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4092 translation of PA4092Homolog Bit Score 332.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene5055 (ORF: BLASTX 150.0), t_PA4092 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCCCAGCTCGAACCCAGGCAGCAAGCCTTCCGCAACGCCATGGCGCATCTTTCGGCGGCGGTCAACGTG 
ATCACCAGCAACGGCCCGGCCGGACGCTGCGGGATCACCGCCACCGCGGTCTGCTCGGTCACCGACAGCCCG 
CCGACGCTGATGCTCTGCATCAACCGCAACAGCGAGATGAACACGGTGTTCAAGGCCAACGGCCGGCTCTGC 
GTGAACGTCCTCAGCGGCGAACACGAAGAGGTGGCCCGCCACTTCGCCGGCATGACCGAGGTCCCGATGGAA 
CGCCGCTTCGCCCTCCACGACTGGCGCGAGGGCCTCGCCGGGTTGCCGGTGCTGCACGGCGCCCTGGCCAAC 
CTGCAGGGACGCATCGCCGAGGTCCAGGAGATCGGCACCCATTCGGTGCTGTTGCTGGAGCTGGAGGACATC 
CAGGTCCTCGAACAGGGCGACGGCCTGGTCTACTTCAGCCGCAGCTTCCATCGCCTGCAGCGAGCCTGCCGA 
GCCGCCTGA