ViewGene
Locus Name:
GeneID:
Location:

GeneID:4277
TrivialName: PA14_04580
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_04580
PAO1 Ortholog Locus:PA0350
Sequence Length:507
Protein Length:168
Start: 407469
Stop: 407975
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03739.1|AE004473_1 (AE004473) dihydrofolate reductase [Pseudomonas aeruginosa PAO1]
Identities = 168/168 (100%), Positives = 168/168 (100%)
Sequence:
ATGGCACGACCGCTGGCGATGATCGCCGCCCTTGGCGAGAACCGCGCGATCGGCATCGACAACCGCCTGCCC
TGGCGCCTGCCGGCCGACCTCAAGCATTTCAAGGCGATGACCCTCGGCAAGCCGGTGATCATGGGGCGCAAG
ACCTGGGACTCGCTGGGTCGGCCGCTGCCGGGCCGGCTCAACCTGGTGGTCAGCCGCCAGGCCGGCCTGGCA
CTGGAGGGTGCGGAAGTGTTCGCCAGCCTGGACGCCGCGCTGGCGCGCGCCGAGGCGTGGGCGCAGGCGGAG
GACGCCGACGAACTGATGCTGATCGGCGGCGCCCAGCTCTATGCCGAGGCCTTGCCGCGCGCCGCGCGCCTC
TACCTGACCCGGGTGGGCCTGGCGCCGGAAGGCGACGCCTTTTTCCCCGAGATCGATGGCGCGGCCTGGCGC
CTGGCGTCGAGCATCGAACACGCCGCGGCTGACGATGCGCCTGCCTACGCCTTCGAGGTATGGGAGCGCCGC
TGA
Translation:
MARPLAMIAALGENRAIGIDNRLPWRLPADLKHFKAMTLGKPVIMGRKTWDSLGRPLPGRLNLVVSRQAGLA
LEGAEVFASLDAALARAEAWAQAEDADELMLIGGAQLYAEALPRAARLYLTRVGLAPEGDAFFPEIDGAAWR
LASSIEHAAADDAPAYAFEVWERR*
AnnotationID:4283GeneID:4277
AnnotatorUID: diggins
Modification Date/Time: 2005-03-24 18:06:12
Gene Name:folA
Confidence Code:2
GeneProduct:dihydrofolate reductase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:cd00209,pfam00186,COG0262
Secondary Functional Category(ies):biosynthesis of cofactors, prosthetic groups and carriers
EC Number:1.5.1.3
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO53981.1| (AE016857) dihydrofolate reductase [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 170

 Score =  251 bits (642), Expect = 1e-66
 Identities = 120/164 (73%), Positives = 137/164 (83%)

>gb|AAN70697.1|AE016713_3 (AE016793) dihydrofolate reductase [Pseudomonas putida KT2440]
          Length = 171

 Score =  224 bits (570), Expect = 2e-58
 Identities = 112/166 (67%), Positives = 129/166 (77%), Gaps = 3/166 (1%)

>gb|AAL51790.1| (AE009503) DIHYDROFOLATE REDUCTASE [Brucella melitensis 16M]
          Length = 172

 Score =  170 bits (430), Expect = 4e-42
 Identities = 89/170 (52%), Positives = 116/170 (68%), Gaps = 2/170 (1%)






 
Structural Features:
cd00209, DHFR, Dihydrofolate reductase (DHFR).
CD-Length = 158 residues, 100.0% aligned
Score =  205 bits (523), Expect = 2e-54

pfam00186, DiHfolate_red, Dihydrofolate reductase.
CD-Length = 173 residues, 100.0% aligned
Score =  176 bits (448), Expect = 9e-46
Identities = 84/174 (48%), Positives = 104/174 (59%), Gaps = 11/174 (6%)

COG0262, FolA, Dihydrofolate reductase [Coenzyme metabolism]
CD-Length = 167 residues,  99.4% aligned
Score =  155 bits (392), Expect = 3e-39
Identities = 82/171 (47%), Positives = 101/171 (59%), Gaps = 12/171 (7%)






   
Genomic Context:






   
Comment:


















    ReferenceID:8555




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:25697




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33691




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4277_cdd.html


    		




 
URL:

















    ReferenceID:35410




  
Author/Investigator(s):
        Azakami H, Sugino H, Murooka Y.





  
Title:
        Cloning and nucleotide sequence of a negative regulator gene for Klebsiella aerogenes arylsulfatase synthesis and identification of the gene as folA.




  
PubMed:
        1551851


MedLine:
        




    
Source:
J Bacteriol. 1992 Apr;174(7):2344-51.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1551851

















    ReferenceID:35411




  
Author/Investigator(s):
        Zywno-van Ginkel S, Dooley TP, Suling WJ, Barrow WW.





  
Title:
        Identification and cloning of the Mycobacterium avium folA gene, required for dihydrofolate reductase activity.




  
PubMed:
        9368362


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1997 Nov 1;156(1):69-78.

Erratum in:
    * FEMS Microbiol Lett 1998 Jan 15;158(2):279.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9368362









           
Homologs By Global Alignment 

           

       



     
Gene ID:4277
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6589
                           PA0350_tr
                           translation of PA0350
                           168
                           99.99
                           99.99
                           0
                           858.0
     

     
     
177993
                           gb|AAG03739.1|AE004473_1
                           (AE004473) dihydrofolate reductase [Pseudomonas aeruginosa PAO1]
                           168
                           99.99
                           99.99
                           0
                           858.0
     

     
     
177994
                           gb|AAO53981.1|
                           (AE016857) dihydrofolate reductase [Pseudomonas syringae pv. tomato str. DC3000]
                           170
                           70.58
                           80.58
                           2
                           634.0
     

     
     
177995
                           gb|AAN70697.1|AE016713_3
                           (AE016793) dihydrofolate reductase [Pseudomonas putida KT2440]
                           172
                           65.11
                           75.58
                           5
                           570.0
     

     
     
177996
                           gb|AAL51790.1|
                           (AE009503) DIHYDROFOLATE REDUCTASE [Brucella melitensis 16M]
                           173
                           52.02
                           67.05
                           6
                           435.0
     

     






      

          
GC ORFID: 47279How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0350Glimmer Score: 

          
Start: 6208811Stop: 6209317

          
Length: 507


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0350 translation of PA0350Homolog Bit Score 335.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6810 (ORF: BLASTX 253.0), t_PA0350 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCACGACCGCTGGCGATGATCGCCGCCCTTGGCGAGAACCGCGCGATCGGCATCGACAACCGCCTGCCC 
TGGCGCCTGCCGGCCGACCTCAAGCATTTCAAGGCGATGACCCTCGGCAAGCCGGTGATCATGGGGCGCAAG 
ACCTGGGACTCGCTGGGTCGGCCGCTGCCGGGCCGGCTCAACCTGGTGGTCAGCCGCCAGGCCGGCCTGGCA 
CTGGAGGGTGCGGAAGTGTTCGCCAGCCTGGACGCCGCGCTGGCGCGCGCCGAGGCGTGGGCGCAGGCGGAG 
GACGCCGACGAACTGATGCTGATCGGCGGCGCCCAGCTCTATGCCGAGGCCTTGCCGCGCGCCGCGCGCCTC 
TACCTGACCCGGGTGGGCCTGGCGCCGGAAGGCGACGCCTTTTTCCCCGAGATCGATGGCGCGGCCTGGCGC 
CTGGCGTCGAGCATCGAACACGCCGCGGCTGACGATGCGCCTGCCTACGCCTTCGAGGTATGGGAGCGCCGC 
TGA