ViewGene
Locus Name:
GeneID:
Location:

GeneID:425
TrivialName: PA14_47800
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_47800
PAO1 Ortholog Locus:PA1271
Sequence Length:1851
Protein Length:616
Start: 4252792
Stop: 4250942
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04660.1|AE004557_1 (AE004557) probable tonB-dependent receptor [Pseudomonas aeruginosa PAO1]
Identities = 613/616 (99%), Positives = 615/616 (99%)
Sequence:
ATGAACAGAGTTTTCCTGACCCCGGCAGCCGTGGCGCTGTGCGGCGCGTCTTCCCTGAGCCTGGCCGAACCG
GTCAGCCTTGTCGACCAGGTGGTGACCGCCACCCGTACCGCCCAGACCGCTTCCCAGAGCCTGGCGGCGGTG
AGCGTCATCGACCGCGAGGATATCGAGCGCAGCCAGGCGCGCAGCGTGCCGGAGCTGTTGCGCCAGGTACCC
GGCGTGTCGCTGGCGAACAACGGCGGTTTCGGCAAGAACACCACGCTGTTCCTGCGCGGCACCGAGTCCGAC
CATGTGCTGGTGCTGATCGACGGCATCAAGGTCGGCTCGGCCAGCGCCGGCCTCACCGCGTTCCAGGACTTG
CCGGTGGAGCTGATCGAGCGCATCGAGGTGGTCCGCGGGCCGCGTTCCAGCCTGTACGGCTCGGAGGCCATC
GGCGGGGTGATCCAGATCTTCACCCGGCGCGGCGACGGCCAGGGCGCCAAGCCGTTCTTCTCCGCCGGTTAC
GGCACCCATCAGACCCTGGAGGGCAGCGCCGGGGTCAGCGGCGGCGCTGGCAACGGCTGGTACAGCCTCGGG
GTGAGCAGTTTCGATACGGCGGGGATCAACACCAAGCGCGCCGGTACTGCGGGCTATGAGCCAGACCGAGAC
GGCTACCGCAACCTGTCCGGCAACCTGCGCGGCGGCTATCGCTTCGACAATGGCCTGGAACTCGACGGCACC
CTGCTCAGGGCCAAGTCGCACAACGACTATGACCAGGTCTTCGGCAACTCCGGTTTCAATGCCAACGCCGAC
GGCGAGCAGAACCTGGTCGGCGGCCGTGCCCGCTTCACTCCGTTCGATCCCTGGCTGGTGACCCTCCAGGCC
GGGCGCAGCGAGGACAAGGCCGATGCCTATCAGGATGGCCGTTTCTACTCGCGCTTCGATACCCGTCGCGAC
AGCCTGTCCTGGCAGAACGACCTGACCCTGGCCGAAGGCCATGTGCTGACCCTCGGCTACGACTGGCAGAAG
GACGAGATCAGCAGCAGCGAAGCCTTCAGCGTCGACTCGCGGCTGAACAAAGGCTGGTTCGCCCAGTACCTC
GGCCGGTACGGTCGCCAGGATTGGCAACTGAGCCTGCGCCGCGACGACAACCAGCAGTTCGGCGTGCACGAT
ACCGGCAGCGCCGCCTGGGGCTACGCGCTGAGCGACGCGCTGCGCTTCACCGTCAGCTACGGCACGGCGTTC
AAGGCGCCGACCTTCAACGAACTCTACTACCCCGACTACGGCAATCCCGACCTGGACGCCGAGACTTCGCGC
AGCCTGGAAGTCGGGCTGAGCGGTACGCATGGCTGGGGGCACTGGGCGGTGAATGCCTTCCGTACCAACGTC
GACGACCTGATCGGCAACGATCCGCGTCCGGCGCCGGGGCGCCCCTGGGGGCAGCCGAACAACATCGACGAA
GCGCGCATCCGTGGCGTCGAACTGGTCCTCGGCAGCCAGTGGCTGGGCTGGGACTGGAACGCCAACGCAACC
TTCCTCGACCCGCAAAACCGTTCCGGCGGCGTCAACGACGGCAACGAGCTGCCGCGCCGGGCGCGGCGGATG
TTCAACCTGGAACTGGACCGGCGCTTCGAGCGTCTTTCGCTGGGCGCCAGCGTGCACGCCGAAGGCCGACGC
TATGATGACCCGGCCAACAAGGTGCGCCTGGGCGGCTACGCCACCCTCGACCTGCGCAGCGAGTACCGGCTG
AACGACGAATGGCGCCTGCAGGGCCGGATCGCCAACCTGTTCGGTGCCGACTACGAAACCGCGTATGGCTAC
AACCAGCCTGGCCAGGCGGTCTACCTCAGCGTGCGCTACCAGGCCCTGTGA
Translation:
MNRVFLTPAAVALCGASSLSLAEPVSLVDQVVTATRTAQTASQSLAAVSVIDREDIERSQARSVPELLRQVP
GVSLANNGGFGKNTTLFLRGTESDHVLVLIDGIKVGSASAGLTAFQDLPVELIERIEVVRGPRSSLYGSEAI
GGVIQIFTRRGDGQGAKPFFSAGYGTHQTLEGSAGVSGGAGNGWYSLGVSSFDTAGINTKRAGTAGYEPDRD
GYRNLSGNLRGGYRFDNGLELDGTLLRAKSHNDYDQVFGNSGFNANADGEQNLVGGRARFTPFDPWLVTLQA
GRSEDKADAYQDGRFYSRFDTRRDSLSWQNDLTLAEGHVLTLGYDWQKDEISSSEAFSVDSRLNKGWFAQYL
GRYGRQDWQLSLRRDDNQQFGVHDTGSAAWGYALSDALRFTVSYGTAFKAPTFNELYYPDYGNPDLDAETSR
SLEVGLSGTHGWGHWAVNAFRTNVDDLIGNDPRPAPGRPWGQPNNIDEARIRGVELVLGSQWLGWDWNANAT
FLDPQNRSGGVNDGNELPRRARRMFNLELDRRFERLSLGASVHAEGRRYDDPANKVRLGGYATLDLRSEYRL
NDEWRLQGRIANLFGADYETAYGYNQPGQAVYLSVRYQAL*
AnnotationID:434GeneID:425
AnnotatorUID: diggins
Modification Date/Time: 2005-02-22 17:05:56
Gene Name:
Confidence Code:3
GeneProduct:putative tonB-dependent receptor
Cell Localization:()
Synonyms:btuB
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:putative outer membrane receptor for transport of vitamin B
Functional Category Confidence Code:5
COGs:COG4206,COG4771,cd01347
Secondary Functional Category(ies):membrane protein
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAD84547.1| (BX321858) TonB-dependent receptor protein [Nitrosomonas europaea
           ATCC 19718]
          Length = 612

 Score =  602 bits (1553), Expect = e-171
 Identities = 303/604 (50%), Positives = 417/604 (69%), Gaps = 17/604 (2%)

>gb|AAM42338.1| (AE012421) outer membrane receptor for transport of vitamin B
           [Xanthomonas campestris pv. campestris str. ATCC 33913]
          Length = 626

 Score =  576 bits (1484), Expect = e-163
 Identities = 314/612 (51%), Positives = 401/612 (65%), Gaps = 16/612 (2%)








 
Structural Features:
COG4206, BtuB, Outer membrane cobalamin receptor protein 
[Coenzyme metabolism]
CD-Length = 608 residues, 100.0% aligned
Score =  703 bits (1815), Expect = 0.0
Identities = 301/624 (48%), Positives = 353/624 (56%), Gaps = 25/624 (4%)

COG4771, FepA, Outer membrane receptor for ferrienterochelin and colicins 
[Inorganic ion transport and metabolism]
CD-Length = 699 residues,  98.7% aligned
Score =  239 bits (611), Expect = 7e-64
Identities = 181/701 (25%), Positives = 281/701 (40%), Gaps = 100/701 (14%)

cd01347, ligand_gated_channel, TonB dependent/Ligand-Gated channel
CD-Length = 672 residues,  99.7% aligned
Score =  215 bits (548), Expect = 1e-56






   
Genomic Context:






   
Comment:


















    ReferenceID:853




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17995




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37524




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:425_cdd.html


    		




 
URL:

















    ReferenceID:38036




  
Author/Investigator(s):
        Heller K, Kadner RJ.





  
Title:
        Nucleotide sequence of the gene for the vitamin B12 receptor protein in the outer membrane of Escherichia coli.




  
PubMed:
        3882670


MedLine:
        




    
Source:
J Bacteriol. 1985 Mar;161(3):904-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3882670









           
Homologs By Global Alignment 

           

       



     
Gene ID:425
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
715
                           PA1271_tr
                           translation of PA1271
                           616
                           99.51
                           99.83
                           0
                           3235.0
     

     
     
32230
                           gb|AAG04660.1|AE004557_1
                           (AE004557) probable tonB-dependent receptor [Pseudomonas aeruginosa PAO1]
                           616
                           99.51
                           99.83
                           0
                           3235.0
     

     
     
32232
                           gb|AAM42338.1|
                           (AE012421) outer membrane receptor for transport of vitamin B [Xanthomonas campestris pv. campestris str. ATCC 33913]
                           636
                           50.47
                           65.09
                           30
                           1504.0
     

     
     
32231
                           emb|CAD84547.1|
                           (BX321858) TonB-dependent receptor protein [Nitrosomonas europaea ATCC 19718]
                           624
                           48.87
                           67.46
                           20
                           1562.0
     

     
     
32233
                           gb|AAM38038.1|
                           (AE011964) outer membrane receptor for transport of vitamin B [Xanthomonas axonopodis pv. citri str. 306]
                           633
                           48.81
                           63.19
                           24
                           1468.0
     

     






      

          
GC ORFID: 43427How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1271Glimmer Score: 

          
Start: 701117Stop: 702967

          
Length: 1851


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1271 translation of PA1271Homolog Bit Score 1250.0

          
Other Homologs:  t_PA1271 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACAGAGTTTTCCTGACCCCGGCAGCCGTGGCGCTGTGCGGCGCGTCTTCCCTGAGCCTGGCCGAACCG 
GTCAGCCTTGTCGACCAGGTGGTGACCGCCACCCGTACCGCCCAGACCGCTTCCCAGAGCCTGGCGGCGGTG 
AGCGTCATCGACCGCGAGGATATCGAGCGCAGCCAGGCGCGCAGCGTGCCGGAGCTGTTGCGCCAGGTACCC 
GGCGTGTCGCTGGCGAACAACGGCGGTTTCGGCAAGAACACCACGCTGTTCCTGCGCGGCACCGAGTCCGAC 
CATGTGCTGGTGCTGATCGACGGCATCAAGGTCGGCTCGGCCAGCGCCGGCCTCACCGCGTTCCAGGACTTG 
CCGGTGGAGCTGATCGAGCGCATCGAGGTGGTCCGCGGGCCGCGTTCCAGCCTGTACGGCTCGGAGGCCATC 
GGCGGGGTGATCCAGATCTTCACCCGGCGCGGCGACGGCCAGGGCGCCAAGCCGTTCTTCTCCGCCGGTTAC 
GGCACCCATCAGACCCTGGAGGGCAGCGCCGGGGTCAGCGGCGGCGCTGGCAACGGCTGGTACAGCCTCGGG 
GTGAGCAGTTTCGATACGGCGGGGATCAACACCAAGCGCGCCGGTACTGCGGGCTATGAGCCAGACCGAGAC 
GGCTACCGCAACCTGTCCGGCAACCTGCGCGGCGGCTATCGCTTCGACAATGGCCTGGAACTCGACGGCACC 
CTGCTCAGGGCCAAGTCGCACAACGACTATGACCAGGTCTTCGGCAACTCCGGTTTCAATGCCAACGCCGAC 
GGCGAGCAGAACCTGGTCGGCGGCCGTGCCCGCTTCACTCCGTTCGATCCCTGGCTGGTGACCCTCCAGGCC 
GGGCGCAGCGAGGACAAGGCCGATGCCTATCAGGATGGCCGTTTCTACTCGCGCTTCGATACCCGTCGCGAC 
AGCCTGTCCTGGCAGAACGACCTGACCCTGGCCGAAGGCCATGTGCTGACCCTCGGCTACGACTGGCAGAAG 
GACGAGATCAGCAGCAGCGAAGCCTTCAGCGTCGACTCGCGGCTGAACAAAGGCTGGTTCGCCCAGTACCTC 
GGCCGGTACGGTCGCCAGGATTGGCAACTGAGCCTGCGCCGCGACGACAACCAGCAGTTCGGCGTGCACGAT 
ACCGGCAGCGCCGCCTGGGGCTACGCGCTGAGCGACGCGCTGCGCTTCACCGTCAGCTACGGCACGGCGTTC 
AAGGCGCCGACCTTCAACGAACTCTACTACCCCGACTACGGCAATCCCGACCTGGACGCCGAGACTTCGCGC 
AGCCTGGAAGTCGGGCTGAGCGGTACGCATGGCTGGGGGCACTGGGCGGTGAATGCCTTCCGTACCAACGTC 
GACGACCTGATCGGCAACGATCCGCGTCCGGCGCCGGGGCGCCCCTGGGGGCAGCCGAACAACATCGACGAA 
GCGCGCATCCGTGGCGTCGAACTGGTCCTCGGCAGCCAGTGGCTGGGCTGGGACTGGAACGCCAACGCAACC 
TTCCTCGACCCGCAAAACCGTTCCGGCGGCGTCAACGACGGCAACGAGCTGCCGCGCCGGGCGCGGCGGATG 
TTCAACCTGGAACTGGACCGGCGCTTCGAGCGTCTTTCGCTGGGCGCCAGCGTGCACGCCGAAGGCCGACGC 
TATGATGACCCGGCCAACAAGGTGCGCCTGGGCGGCTACGCCACCCTCGACCTGCGCAGCGAGTACCGGCTG 
AACGACGAATGGCGCCTGCAGGGCCGGATCGCCAACCTGTTCGGTGCCGACTACGAAACCGCGTATGGCTAC 
AACCAGCCTGGCCAGGCGGTCTACCTCAGCGTGCGCTACCAGGCCCTGTGA