ViewGene
Locus Name:
GeneID:
Location:

GeneID:4246
TrivialName: PA14_29980
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_29980
PAO1 Ortholog Locus:PA2640
Sequence Length:501
Protein Length:166
Start: 2596162
Stop: 2595662
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06028.1|AE004692_7 (AE004692) NADH dehydrogenase I chain E [Pseudomonas aeruginosa PAO1]
Identities = 166/166 (100%), Positives = 166/166 (100%)
Sequence:
ATGAGCCAGAGCAACCTGATTCAGACCGACCGTTTCGTCCTCAGCGAAACCGAGCGCTCGTCCATCGAGCAT
GAAATGCATCACTACGAGGACCCGCGCGCGGCGTCCATCGAAGCCCTGAAGATCGTCCAGAAGCAGCGCGGC
TGGGTGCCGGACGGCGCCATCCCGGCGATCGGCGAGGTGCTGGGGATCCCGGCCAGCGACGTCGAAGGCGTC
GCCACCTTCTATAGTCAGATATTCCGCCAGCCGGTGGGCCGCCACATCATTCGCGTCTGCGACAGCATGGTC
TGCTACATCGGCGGCCACGAGTCGGTGGTCGGCGAGATCCAGAAGCAACTCGGCATCGGTCTCGGCCAGACT
ACCGCCGACGGACGCTTCACCCTGCTCCCGGTCTGCTGCCTGGGCAACTGCGACAAGGCCCCGGCGCTGATG
ATCGACGACGACACCCATGGCGACGTGCGTCCCGACGGTGTCGCCAAACTGCTGGAGGCCTACGTATGA
Translation:
MSQSNLIQTDRFVLSETERSSIEHEMHHYEDPRAASIEALKIVQKQRGWVPDGAIPAIGEVLGIPASDVEGV
ATFYSQIFRQPVGRHIIRVCDSMVCYIGGHESVVGEIQKQLGIGLGQTTADGRFTLLPVCCLGNCDKAPALM
IDDDTHGDVRPDGVAKLLEAYV*
AnnotationID:4252GeneID:4246
AnnotatorUID: diggins
Modification Date/Time: 2005-04-29 11:11:23
Gene Name:nuoE
Confidence Code:2
GeneProduct:NADH dehydrogenase I chain E
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1905,pfam01257
Secondary Functional Category(ies):
EC Number:1.6.5.3
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69705.1|AE016606_8 (AE016789) NADH dehydrogenase I, E subunit [Pseudomonas putida
           KT2440]
          Length = 165

 Score =  306 bits (785), Expect = 2e-83
 Identities = 149/163 (91%), Positives = 155/163 (95%)

>gb|AAO56846.1| (AE016868) NADH dehydrogenase I, E subunit [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 165

 Score =  291 bits (746), Expect = 8e-79
 Identities = 140/163 (85%), Positives = 154/163 (94%)

>gb|AAG57414.1|AE005460_8 (AE005460) NADH dehydrogenase I chain E [Escherichia coli O157:H7
           EDL933]
          Length = 166

 Score =  246 bits (629), Expect = 3e-65
 Identities = 115/165 (69%), Positives = 136/165 (82%)






 
Structural Features:
COG1905, NuoE, NADH:ubiquinone oxidoreductase 24 kD subunit 
[Energy production and conversion]
CD-Length = 160 residues,  96.2% aligned
Score =  215 bits (548), Expect = 3e-57
Identities = 73/154 (47%), Positives = 101/154 (65%)

pfam01257, Complex1_24kDa, Respiratory-chain NADH dehydrogenase 24 Kd subunit.
CD-Length = 157 residues,  99.4% aligned
Score =  200 bits (511), Expect = 5e-53
Identities = 68/156 (43%), Positives = 93/156 (59%), Gaps = 2/156 (1%)







   
Genomic Context:






   
Comment:


















    ReferenceID:8493




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:25635




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33223




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4246_cdd.html


    		




 
URL:

















    ReferenceID:34575




  
Author/Investigator(s):
        Camacho Carvajal MM, Wijfjes AH, Mulders IH, Lugtenberg BJ, Bloemberg GV.





  
Title:
        Characterization of NADH dehydrogenases of Pseudomonas fluorescens WCS365 and their role in competitive root colonization.




  
PubMed:
        12118882


MedLine:
        




    
Source:
Mol Plant Microbe Interact. 2002 Jul;15(7):662-71.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12118882

















    ReferenceID:34587




  
Author/Investigator(s):
        Herter SM, Schiltz E, Drews G.





  
Title:
        Protein and gene structure of the NADH-binding fragment of Rhodobacter capsulatus NADH:ubiquinone oxidoreductase.




  
PubMed:
        9219542


MedLine:
        




    
Source:
Eur J Biochem. 1997 Jun 15;246(3):800-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9219542









           
Homologs By Global Alignment 

           

       



     
Gene ID:4246
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6542
                           PA2640_tr
                           translation of PA2640
                           166
                           99.99
                           99.99
                           0
                           869.0
     

     
     
177411
                           gb|AAG06028.1|AE004692_7
                           (AE004692) NADH dehydrogenase I chain E [Pseudomonas aeruginosa PAO1]
                           166
                           99.99
                           99.99
                           0
                           869.0
     

     
     
177412
                           gb|AAN69705.1|AE016606_8
                           (AE016789) NADH dehydrogenase I, E subunit [Pseudomonas putida KT2440]
                           166
                           89.75
                           93.37
                           1
                           784.0
     

     
     
177413
                           gb|AAF97801.1|AF281148_5
                           (AF281148) NADH dehydrogenase I subunit E [Pseudomonas fluorescens]
                           166
                           86.74
                           92.16
                           0
                           760.0
     

     
     
177414
                           gb|AAO56846.1|
                           (AE016868) NADH dehydrogenase I, E subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           166
                           84.33
                           92.77
                           1
                           745.0
     

     






      

          
GC ORFID: 47248How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2640Glimmer Score: 

          
Start: 2357764Stop: 2358264

          
Length: 501


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2640 translation of PA2640Homolog Bit Score 339.0

          
Other Homologs:  psyr_15may02_Scaffold17_revised_gene2767 (ORF: BLASTX 295.0), t_PA2640 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCCAGAGCAACCTGATTCAGACCGACCGTTTCGTCCTCAGCGAAACCGAGCGCTCGTCCATCGAGCAT 
GAAATGCATCACTACGAGGACCCGCGCGCGGCGTCCATCGAAGCCCTGAAGATCGTCCAGAAGCAGCGCGGC 
TGGGTGCCGGACGGCGCCATCCCGGCGATCGGCGAGGTGCTGGGGATCCCGGCCAGCGACGTCGAAGGCGTC 
GCCACCTTCTATAGTCAGATATTCCGCCAGCCGGTGGGCCGCCACATCATTCGCGTCTGCGACAGCATGGTC 
TGCTACATCGGCGGCCACGAGTCGGTGGTCGGCGAGATCCAGAAGCAACTCGGCATCGGTCTCGGCCAGACT 
ACCGCCGACGGACGCTTCACCCTGCTCCCGGTCTGCTGCCTGGGCAACTGCGACAAGGCCCCGGCGCTGATG 
ATCGACGACGACACCCATGGCGACGTGCGTCCCGACGGTGTCGCCAAACTGCTGGAGGCCTACGTATGA