ViewGene
Locus Name:
GeneID:
Location:

GeneID:4241
TrivialName: PA14_14770
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_14770
PAO1 Ortholog Locus:PA3811
Sequence Length:522
Protein Length:173
Start: 1256352
Stop: 1256873
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07198.1|AE004799_4 (AE004799) heat shock protein HscB [Pseudomonas aeruginosa PAO1]
Identities = 173/173 (100%), Positives = 173/173 (100%)
Sequence:
GTGGGTAAACCCTGTCATTTCGCCCAGTTCGACCTGCAACCGGCTTTCCTGGTCGATCTCGACGAGCTCGGC
CAGCGCTATCGCGAGTTGGTGCGCAGCGTCCATCCCGACCGTTTCGCCGACGCTCCCGAGCGCGAGCAGCGT
CTCGCCCTGGAACGCGCGGCGCAACTGAACGAGGCCTACCAGACCCTGAAGAGCGCTCCGCGCCGGGCCCTG
TACCTGTTGACCCTGAGTGGTCACGAGTTGCCCTTGGAGGCTACGGTGCAGGATCCGGAGTTTCTCCTGCAG
CAGATGCAGCTGCGCGAGGAACTGGAGGAGTTGCAGGACAGCGCCGACCTGGCCGGCGTGGCGACCTTCAAG
CGTCGGCTCAAGGCCGCCCAGGCCGAGCTGGAGCGCGAGTTCGCCGCGTGCTGGGACGATGCGCAGCGCCGC
GAAGAGGCCGAACGCCTGGTCCGTCGCATGCAGTTCCTCGACAAGCTCGCGCAGGAAGTGCGCCAGCTGGAA
GAGCGACTCGACGATTAA
Translation:
MGKPCHFAQFDLQPAFLVDLDELGQRYRELVRSVHPDRFADAPEREQRLALERAAQLNEAYQTLKSAPRRAL
YLLTLSGHELPLEATVQDPEFLLQQMQLREELEELQDSADLAGVATFKRRLKAAQAELEREFAACWDDAQRR
EEAERLVRRMQFLDKLAQEVRQLEERLDD*
AnnotationID:4247GeneID:4241
AnnotatorUID: diggins
Modification Date/Time: 2005-04-24 12:12:18
Gene Name:hscB
Confidence Code:2
GeneProduct:heat shock protein HscB
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(9) Chaperones & heat shock proteins
Alternate Gene Product Name:co-chaperone Hsc20
Functional Category Confidence Code:5
COGs:COG1076,KOG3192
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO54947.1| (AE016860) co-chaperone Hsc20 [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 173

 Score =  271 bits (694), Expect = 1e-72
 Identities = 132/173 (76%), Positives = 152/173 (87%)

>gb|AAC24479.1| (AF010139) heat-shock-cognate protein; HscB [Azotobacter
           vinelandii]
          Length = 171

 Score =  241 bits (615), Expect = 1e-63
 Identities = 125/168 (74%), Positives = 136/168 (80%)








 
Structural Features:
COG1076, DjlA, DnaJ-domain-containing proteins 1 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 174 residues,  98.3% aligned
Score = 84.6 bits (209), Expect = 1e-17
Identities = 64/172 (37%), Positives = 101/172 (58%), Gaps = 4/172 (2%)

KOG3192, Mitochondrial J-type chaperone 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 168 residues,  91.7% aligned
Score = 71.6 bits (175), Expect = 9e-14
Identities = 48/159 (30%), Positives = 85/159 (53%), Gaps = 6/159 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:15052




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:25625




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31745




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4241_cdd.html


    		




 
URL:

















    ReferenceID:34298




  
Author/Investigator(s):
        Campos-Garcia J, G Ordonez L, Soberon-Chavez G.





  
Title:
        The Pseudomonas aeruginosa hscA gene encodes Hsc66, a DnaK homologue.




  
PubMed:
        10846221


MedLine:
        




    
Source:
Microbiology. 2000 Jun;146 ( Pt 6):1429-35.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10846221

















    ReferenceID:34299




  
Author/Investigator(s):
        Vickery LE, Silberg JJ, Ta DT.





  
Title:
        Hsc66 and Hsc20, a new heat shock cognate molecular chaperone system from Escherichia coli.




  
PubMed:
        9144776


MedLine:
        




    
Source:
Protein Sci. 1997 May;6(5):1047-56.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9144776

















    ReferenceID:34300




  
Author/Investigator(s):
        Takahashi Y, Nakamura M.





  
Title:
        Functional assignment of the ORF2-iscS-iscU-iscA-hscB-hscA-fdx-ORF3 gene cluster involved in the assembly of Fe-S clusters in Escherichia coli.




  
PubMed:
        10544286


MedLine:
        




    
Source:
J Biochem (Tokyo). 1999 Nov;126(5):917-26.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10544286









           
Homologs By Global Alignment 

           

       



     
Gene ID:4241
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6538
                           PA3811_tr
                           translation of PA3811
                           173
                           99.99
                           99.99
                           0
                           872.0
     

     
     
177371
                           gb|AAG07198.1|AE004799_4
                           (AE004799) heat shock protein HscB [Pseudomonas aeruginosa PAO1]
                           173
                           99.42
                           99.99
                           0
                           869.0
     

     
     
177372
                           gb|AAC79496.2|
                           (AF096864) heat shock protein [Pseudomonas aeruginosa]
                           173
                           79.76
                           87.28
                           0
                           703.0
     

     
     
177373
                           gb|AAO54947.1|
                           (AE016860) co-chaperone Hsc20 [Pseudomonas syringae pv. tomato str. DC3000]
                           173
                           75.72
                           87.86
                           0
                           690.0
     

     
     
177374
                           gb|AAN66470.1|AE016276_5
                           (AE016777) co-chaperone Hsc20 [Pseudomonas putida KT2440]
                           173
                           73.41
                           83.81
                           0
                           663.0
     

     






      

          
GC ORFID: 47243How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3811Glimmer Score: 

          
Start: 3697586Stop: 3697065

          
Length: 522


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA3811 translation of PA3811Homolog Bit Score 340.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4520 (ORF: BLASTX 271.0), t_PA3811 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGGGTAAACCCTGTCATTTCGCCCAGTTCGACCTGCAACCGGCTTTCCTGGTCGATCTCGACGAGCTCGGC 
CAGCGCTATCGCGAGTTGGTGCGCAGCGTCCATCCCGACCGTTTCGCCGACGCTCCCGAGCGCGAGCAGCGT 
CTCGCCCTGGAACGCGCGGCGCAACTGAACGAGGCCTACCAGACCCTGAAGAGCGCTCCGCGCCGGGCCCTG 
TACCTGTTGACCCTGAGTGGTCACGAGTTGCCCTTGGAGGCTACGGTGCAGGATCCGGAGTTTCTCCTGCAG 
CAGATGCAGCTGCGCGAGGAACTGGAGGAGTTGCAGGACAGCGCCGACCTGGCCGGCGTGGCGACCTTCAAG 
CGTCGGCTCAAGGCCGCCCAGGCCGAGCTGGAGCGCGAGTTCGCCGCGTGCTGGGACGATGCGCAGCGCCGC 
GAAGAGGCCGAACGCCTGGTCCGTCGCATGCAGTTCCTCGACAAGCTCGCGCAGGAAGTGCGCCAGCTGGAA 
GAGCGACTCGACGATTAA